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hpa017384  (Novus Biologicals)


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    Novus Biologicals hpa017384
    Hpa017384, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpa017384/product/Novus Biologicals
    Average 93 stars, based on 7 article reviews
    hpa017384 - by Bioz Stars, 2026-06
    93/100 stars

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    Image Search Results


    a) Representative lamin B1 and PML confocal immunofluorescence images from human cell lines. The arrows indicate areas of PML accumulation at NE areas devoid of lamina. Scale bar: 10 μm. b) Different condensate types observed upon overexpression of EGFP-PMLII in HeLa cells including widefield immunofluorescent images of lamin B1. Scale bar: 10 μm. c) Quantification of condensate types observed for PMLI or PMLII overexpression in HeLa cells. N=3, for EGFP-PMLI and EGFP-PMLII and N=2 for FLAG-PMLI and FLAG-PMLII. Mean +/-sd is indicated. d) Representative, lamin B1 and sp100 widefield immunofluorescence images of HeLa cells overexpressing EGFP-PMLI or EGFP-PMLII. Scale bar: 10 μm. e) Quantification of relative lamin B1 intensity and sp100 intensity at puncta or patches in HeLa cells overexpressing EGFP-PMLI (N=2 (n=18, 32 cells)) and EGFP-PMLII (puncta; N=3 (n=21,14,13 cells), patches; N=3(n= 11,17,17 cells)). Mean +/-sd are indicated, independent experiments are indicated by the different symbols. P-value was calculated using a one-way ANOVA test followed by Tukey’s post hoc test (**P<0.01, ***P< 0.001). f) Representative, lamin B1 confocal immunofluorescence images of HeLa cells co-overexpressing EGFP-PMLI and FLAG-PMLII. Scale bar: 10 μm. g) Quantification of PMLI/PMLII intensities per condensate type. n=112, 3,6,40, 39 for types 1-5 respectively. Data are presented from three independent experiments indicated by the triangels, squares and cirkels. Median is indicated.

    Journal: bioRxiv

    Article Title: Patching up the nucleus: a novel role for PMLII in nuclear envelope rupture repair

    doi: 10.1101/2025.01.24.634656

    Figure Lengend Snippet: a) Representative lamin B1 and PML confocal immunofluorescence images from human cell lines. The arrows indicate areas of PML accumulation at NE areas devoid of lamina. Scale bar: 10 μm. b) Different condensate types observed upon overexpression of EGFP-PMLII in HeLa cells including widefield immunofluorescent images of lamin B1. Scale bar: 10 μm. c) Quantification of condensate types observed for PMLI or PMLII overexpression in HeLa cells. N=3, for EGFP-PMLI and EGFP-PMLII and N=2 for FLAG-PMLI and FLAG-PMLII. Mean +/-sd is indicated. d) Representative, lamin B1 and sp100 widefield immunofluorescence images of HeLa cells overexpressing EGFP-PMLI or EGFP-PMLII. Scale bar: 10 μm. e) Quantification of relative lamin B1 intensity and sp100 intensity at puncta or patches in HeLa cells overexpressing EGFP-PMLI (N=2 (n=18, 32 cells)) and EGFP-PMLII (puncta; N=3 (n=21,14,13 cells), patches; N=3(n= 11,17,17 cells)). Mean +/-sd are indicated, independent experiments are indicated by the different symbols. P-value was calculated using a one-way ANOVA test followed by Tukey’s post hoc test (**P<0.01, ***P< 0.001). f) Representative, lamin B1 confocal immunofluorescence images of HeLa cells co-overexpressing EGFP-PMLI and FLAG-PMLII. Scale bar: 10 μm. g) Quantification of PMLI/PMLII intensities per condensate type. n=112, 3,6,40, 39 for types 1-5 respectively. Data are presented from three independent experiments indicated by the triangels, squares and cirkels. Median is indicated.

    Article Snippet: The following primary antibodies were used: mouse anti-lamin A/C (Santa Cruz, sc-376248, 1/500), mouse anti-lamin B1 (Santa Cruz, sc-365214, 1/500), mouse anti-FLAG (Sigma Aldrich, F1804, 1/1000), rabbit anti-PML (Abcam, ab179466, 1/500) and rabbit anti-sp100 (Atlas antibodies, HPA016707, 1/500).

    Techniques: Immunofluorescence, Over Expression