Structured Review

TaKaRa hotstar taq polymerase
Hotstar Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hotstar taq polymerase/product/TaKaRa
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
hotstar taq polymerase - by Bioz Stars, 2020-04
92/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets
Article Snippet: .. 2.8 Quantification mRNA by Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis Real-time PCR was performed using SYBR Green PCR Mix (Takara, Dalian, China), containing MgCl2 , dNTP, and Hotstar Taq polymerase (Takara, Dalian, China). .. Two microliters cDNA template was added to a total volume of 25 μL containing 12.5 μL SYBR Green mix, and 1 μM each of forward and reverse primers.

Amplification:

Article Title: Variability of Creatine Metabolism Genes in Children with Autism Spectrum Disorder
Article Snippet: .. SLC6A8 was amplified using Herculase II Fusion Taq polymerase, Hotstar Taq polymerase or TaKaRa La Taq polymerase (Takara Bio Inc., Shiga, Japan). ..

Article Title: Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets
Article Snippet: 2.8 Quantification mRNA by Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis Real-time PCR was performed using SYBR Green PCR Mix (Takara, Dalian, China), containing MgCl2 , dNTP, and Hotstar Taq polymerase (Takara, Dalian, China). .. Primers for HATs and 18S were design with Primer 3 ( http://frodo.wi.mit.edu/primer3/primer3_code.html ) based on porcine sequence to produce an amplification product that spanned at least two exons ( ).

Fluorescence:

Article Title: Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets
Article Snippet: 2.8 Quantification mRNA by Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis Real-time PCR was performed using SYBR Green PCR Mix (Takara, Dalian, China), containing MgCl2 , dNTP, and Hotstar Taq polymerase (Takara, Dalian, China). .. We used the following protocol: 1) denaturation program (15 min at 95°C); 2) amplification and quantification program, repeated 45 cycles (15 s at 95°C, 15 s at 58°C, 15 s at 72°C); 3) melting curve program (60 to 99°C with heating rate of 0.1°C/s and fluorescence measurement).

Polymerase Chain Reaction:

Article Title: ARHI is a novel epigenetic silenced tumor suppressor in sporadic pheochromocytoma
Article Snippet: .. For each reaction, 30 ng of tumor DNA was PCR-amplified for 35 cycles using HotStar Taq Polymerase (Takara). .. Sequences were analyzed by alignment to the UCSC sequence using the DNAMAN6.0 tool (LynnonBiosoft).

Article Title: Variability of Creatine Metabolism Genes in Children with Autism Spectrum Disorder
Article Snippet: In some cases, a second nested amplification was carried out on the first PCR product; some of these primers had a GC clamp at their 5′ end. .. SLC6A8 was amplified using Herculase II Fusion Taq polymerase, Hotstar Taq polymerase or TaKaRa La Taq polymerase (Takara Bio Inc., Shiga, Japan).

Article Title: Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets
Article Snippet: .. 2.8 Quantification mRNA by Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis Real-time PCR was performed using SYBR Green PCR Mix (Takara, Dalian, China), containing MgCl2 , dNTP, and Hotstar Taq polymerase (Takara, Dalian, China). .. Two microliters cDNA template was added to a total volume of 25 μL containing 12.5 μL SYBR Green mix, and 1 μM each of forward and reverse primers.

Mutagenesis:

Article Title: ARHI is a novel epigenetic silenced tumor suppressor in sporadic pheochromocytoma
Article Snippet: Paragraph title: Mutation analysis ... For each reaction, 30 ng of tumor DNA was PCR-amplified for 35 cycles using HotStar Taq Polymerase (Takara).

Next-Generation Sequencing:

Article Title: ARHI is a novel epigenetic silenced tumor suppressor in sporadic pheochromocytoma
Article Snippet: For each reaction, 30 ng of tumor DNA was PCR-amplified for 35 cycles using HotStar Taq Polymerase (Takara). .. Somatic mutations in NF1, HIF2A, RET, VHL and SDHB were detected simultaneously using next generation sequencing (BGI, Beijing).

SYBR Green Assay:

Article Title: Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets
Article Snippet: .. 2.8 Quantification mRNA by Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis Real-time PCR was performed using SYBR Green PCR Mix (Takara, Dalian, China), containing MgCl2 , dNTP, and Hotstar Taq polymerase (Takara, Dalian, China). .. Two microliters cDNA template was added to a total volume of 25 μL containing 12.5 μL SYBR Green mix, and 1 μM each of forward and reverse primers.

Sequencing:

Article Title: ARHI is a novel epigenetic silenced tumor suppressor in sporadic pheochromocytoma
Article Snippet: Mutation analysis We detected germline mutations of RET, VHL, SDHx, FH, MAX and TMEM127 with direct Sanger sequencing, as described previously [ - ] or with newly designed primers ( ). .. For each reaction, 30 ng of tumor DNA was PCR-amplified for 35 cycles using HotStar Taq Polymerase (Takara).

Article Title: Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets
Article Snippet: 2.8 Quantification mRNA by Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis Real-time PCR was performed using SYBR Green PCR Mix (Takara, Dalian, China), containing MgCl2 , dNTP, and Hotstar Taq polymerase (Takara, Dalian, China). .. Primers for HATs and 18S were design with Primer 3 ( http://frodo.wi.mit.edu/primer3/primer3_code.html ) based on porcine sequence to produce an amplification product that spanned at least two exons ( ).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets
Article Snippet: .. 2.8 Quantification mRNA by Real-time reverse transcription polymerase chain reaction (RT-PCR) analysis Real-time PCR was performed using SYBR Green PCR Mix (Takara, Dalian, China), containing MgCl2 , dNTP, and Hotstar Taq polymerase (Takara, Dalian, China). .. Two microliters cDNA template was added to a total volume of 25 μL containing 12.5 μL SYBR Green mix, and 1 μM each of forward and reverse primers.

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    TaKaRa hotstart taq
    Hotstart Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstart taq/product/TaKaRa
    Average 90 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    hotstart taq - by Bioz Stars, 2020-04
    90/100 stars
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    99
    TaKaRa taq polymerase
    Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq polymerase/product/TaKaRa
    Average 99 stars, based on 1159 article reviews
    Price from $9.99 to $1999.99
    taq polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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