hotstar taq plus pcr kit  (Qiagen)


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    Structured Review

    Qiagen hotstar taq plus pcr kit
    Hotstar Taq Plus Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstar taq plus pcr kit/product/Qiagen
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hotstar taq plus pcr kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: HCV Genome-Wide Genetic Analyses in Context of Disease Progression and Hepatocellular Carcinoma
    Article Snippet: Clonal sequencing in the E2 gene Twelve clones encompassing the amino-terminal region of the E2 glycoprotein (aa 384–476 in the HCV polyprotein) that included the hypervariable region 1 (aa 384–410) from each of six HCC and six cirrhotic control patients were cloned for quasispecies analyses. .. HCV sequences were amplified by nested PCR from the cDNAs under high-fidelity conditions employing the Hotstart HiFidelity Polymerase kit (Qiagen).

    Article Title: Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis
    Article Snippet: Polymerase chain reactions (PCRs) were performed with PrimeStar HS DNA Polymerase (TaKaRa) or HotStar HiFidelity Polymerase (Qiagen) under standard conditions. .. Synthetic oligonucleotide synthesis and DNA sequencing for cloning experiments were performed by Genomed S.A., Warsaw.

    Amplification:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: .. The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen). ..

    Article Title: Generation and validation of homozygous fluorescent knock-in cells using CRISPR/Cas9 genome editing
    Article Snippet: .. Oligos for amplification of target sequence and sequencing (see ) HotStar HighFidelity PCR kit (QIAGEN, cat. no. 202605) MinElute Gel Extraction Kit (QIAGEN, cat. no. 28604) .. Cas9-HRP antibody (abcam, cat. no. ab202580) GFP antibody (Roche, cat. no. 11814460001) RFP antibody [3F5] (ChromoTek, cat. no. 3f5) Non-Fat Dry Milk powder DTT (Merck, cat. D0632) cOmplete EDTA-free Protease Inhibitor Tablets (Roche, cat. no.11873580001) PhosStop Tablets (Roche, cat.no.

    Article Title: Intradermal Delivery of Synthetic mRNA Using Hollow Microneedles for Efficient and Rapid Production of Exogenous Proteins in Skin
    Article Snippet: .. Briefly, the plasmid insert was amplified by PCR using the HotStar HiFidelity polymerase kit (QIAGEN, Hilden, Germany) and 0.7 μM of each forward (5′-TTGGACCCTCGTACAGAAGCTAATACG-3′) and reverse primer (5′-T120 -CTTCCTACTCAGGCTTTATTCAAAGACCA-3′). .. Primers were purchased from ELLA Biotech (Martinsried, Germany) in high-performance liquid chromatography (HPLC) grade.

    Article Title: Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing
    Article Snippet: Paragraph title: 16S amplicon pyrosequencing ... Master mixes for PCR reactions contained the Qiagen Hotstar Hi-Fidelity Polymerase Kit (Qiagen, Valencia CA) with a forward primer composed of the Roche Titanium Fusion Primer A (5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’), a 10bp Multiplex Identifier (MID) sequence (Roche, Indianapolis, IN), unique to each sample, and the universal bacterial primer 8F (5'-AGAGTTTGATCCTGGCTCAG-3') ( ).

    Article Title: Temporal changes in the bacterial community of animal feces and their correlation with stable fly oviposition, larval development, and adult fitness
    Article Snippet: The bacterial tag-encoded FLX-Titanium 16S rDNA amplicon parallel pyrosequencing and post sequencing processing were carried out at the Medical Biofilm Research Institute (Lubbock, TX, USA) as described by and . .. A single step reaction with 30 cycles was used and 0.5 U of HotStar HiFidelity Polymerase (Qiagen Inc., Valencia, CA, USA) was added to each reaction.

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: .. Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer. .. PCR conditions were the following: 15 min pre-incubation step at 94°C, 35 cycles of denaturation at 94°C for 30 sec, annealing for 45 sec at the respective AT for each primer pair and extension at 72°C for 45 sec, followed by a final extension step at 72°C for 10 min.

    Article Title: Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5
    Article Snippet: .. CD44s ORF was amplified from LN229 cDNA using HotStar HiFidelity Polymerase Kit (Qiagen Inc., Hilden, Germany). .. CD44v3-10 ORF and CD44s ORF were subcloned into pCAG-cRAP-MAP vector possessing C-terminal RAP/MAP tag and pCAG-ssPA16 vector possessing signal sequence and N-terminal PA16 tag (GLEGGVAMPGAEDDVV), respectively.

    Article Title: HCV Genome-Wide Genetic Analyses in Context of Disease Progression and Hepatocellular Carcinoma
    Article Snippet: .. HCV sequences were amplified by nested PCR from the cDNAs under high-fidelity conditions employing the Hotstart HiFidelity Polymerase kit (Qiagen). ..

    Article Title: DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region
    Article Snippet: .. NGS Methods Barcode region amplifications were conducted using Hotstar Hifidelity polymerase (Qiagen) as per manufacturer’s instructions, and barcode primers with NGS priming site attachments as follows: ITS1 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGKARAAGTCGTAACAAGG-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGTTCAAAGAYTCGATGRTTC-3′ ITS2 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCTTCTCCAGACTACAAT-3′ After initial barcode region amplification, PCR products were purified (AMPure, Agencourt) and pooled, and a library constructed using these based on the Illumina 16S Metagenomic Sequencing Library Preparation. .. Index PCR with Nextera XT Index Kit v2 SetC (Illumina, San Diego, CA, USA) was conducted using KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) as per manufacturer’s instructions.

    Synthesized:

    Article Title: HCV Genome-Wide Genetic Analyses in Context of Disease Progression and Hepatocellular Carcinoma
    Article Snippet: HCV RNAs were isolated and cDNA was synthesized as was done for the direct sequencing. .. HCV sequences were amplified by nested PCR from the cDNAs under high-fidelity conditions employing the Hotstart HiFidelity Polymerase kit (Qiagen).

    Construct:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: Bacterial expression plasmid pET28a-nSREBP-1a (1–403aa), driven by T7 promoter, was constructed by inserting the coding sequence of amino acids (aa) 1–403 of rat SREBP-1a into pET28a vector on the basis of pSREBP-1a (NCBI Reference Sequence: ). .. The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen).

    Article Title: DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region
    Article Snippet: .. NGS Methods Barcode region amplifications were conducted using Hotstar Hifidelity polymerase (Qiagen) as per manufacturer’s instructions, and barcode primers with NGS priming site attachments as follows: ITS1 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGKARAAGTCGTAACAAGG-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGTTCAAAGAYTCGATGRTTC-3′ ITS2 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCTTCTCCAGACTACAAT-3′ After initial barcode region amplification, PCR products were purified (AMPure, Agencourt) and pooled, and a library constructed using these based on the Illumina 16S Metagenomic Sequencing Library Preparation. .. Index PCR with Nextera XT Index Kit v2 SetC (Illumina, San Diego, CA, USA) was conducted using KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) as per manufacturer’s instructions.

    Expressing:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: Paragraph title: 1.1. Bacterial expression plasmid construction ... The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen).

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: Paragraph title: Analysis of allelic expression ... Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer.

    Transformation Assay:

    Article Title: The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target) The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target
    Article Snippet: PCRs were performed using HotStar HiFidelity polymerase kit (Qiagen) in a Perkin‐ MJ Research PTC‐200 Peltier Thermal Cycler. .. E. coli was transformed by heat shock (Froger and Hall, ).

    Derivative Assay:

    Article Title: LIV-1 ZIP Ectodomain Shedding in Prion-Infected Mice Resembles Cellular Response to Transition Metal Starvation
    Article Snippet: ZIP10-HA_d300 (deleted from residue 31 to residue 300), ZIP10-HA_d389 (deleted from residue 36 to residue 389), ZIP10-HA_N341A and ZIP10-HA-(H711A/H715A) were derived from the ZIP10-HA plasmid. .. The HotStar HiFidelity polymerase (Qiagen, Valencia, CA, USA) was used for site-directed mutagenesis.

    High Performance Liquid Chromatography:

    Article Title: Intradermal Delivery of Synthetic mRNA Using Hollow Microneedles for Efficient and Rapid Production of Exogenous Proteins in Skin
    Article Snippet: Briefly, the plasmid insert was amplified by PCR using the HotStar HiFidelity polymerase kit (QIAGEN, Hilden, Germany) and 0.7 μM of each forward (5′-TTGGACCCTCGTACAGAAGCTAATACG-3′) and reverse primer (5′-T120 -CTTCCTACTCAGGCTTTATTCAAAGACCA-3′). .. Primers were purchased from ELLA Biotech (Martinsried, Germany) in high-performance liquid chromatography (HPLC) grade.

    Transfection:

    Article Title: Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5
    Article Snippet: CD44s ORF was amplified from LN229 cDNA using HotStar HiFidelity Polymerase Kit (Qiagen Inc., Hilden, Germany). .. These plasmids were named as pCAG-CD44v3-10 and pCAG-ssPA16-CD44s, respectively, and were transfected into CHO-K1 cells using a Neon transfection system (Thermo Fisher Scientific, Inc., Waltham, MA).

    Activation Assay:

    Article Title: Intradermal Delivery of Synthetic mRNA Using Hollow Microneedles for Efficient and Rapid Production of Exogenous Proteins in Skin
    Article Snippet: Briefly, the plasmid insert was amplified by PCR using the HotStar HiFidelity polymerase kit (QIAGEN, Hilden, Germany) and 0.7 μM of each forward (5′-TTGGACCCTCGTACAGAAGCTAATACG-3′) and reverse primer (5′-T120 -CTTCCTACTCAGGCTTTATTCAAAGACCA-3′). .. PCR was run using the following cycling protocol: initial activation step at 94°C for 3 min, followed by 25 cycles of denaturation at 94°C for 45 s, annealing at 55°C for 1 min, extension at 72°C for 1 min, and final extension at 72°C for 5 min. During the PCR, a poly T-tail of 120 thymidines (T) was added to the insert.

    DNA Sequencing:

    Article Title: Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis
    Article Snippet: Polymerase chain reactions (PCRs) were performed with PrimeStar HS DNA Polymerase (TaKaRa) or HotStar HiFidelity Polymerase (Qiagen) under standard conditions. .. Synthetic oligonucleotide synthesis and DNA sequencing for cloning experiments were performed by Genomed S.A., Warsaw.

    Sequencing:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: .. The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen). ..

    Article Title: Generation and validation of homozygous fluorescent knock-in cells using CRISPR/Cas9 genome editing
    Article Snippet: .. Oligos for amplification of target sequence and sequencing (see ) HotStar HighFidelity PCR kit (QIAGEN, cat. no. 202605) MinElute Gel Extraction Kit (QIAGEN, cat. no. 28604) .. Cas9-HRP antibody (abcam, cat. no. ab202580) GFP antibody (Roche, cat. no. 11814460001) RFP antibody [3F5] (ChromoTek, cat. no. 3f5) Non-Fat Dry Milk powder DTT (Merck, cat. D0632) cOmplete EDTA-free Protease Inhibitor Tablets (Roche, cat. no.11873580001) PhosStop Tablets (Roche, cat.no.

    Article Title: Intradermal Delivery of Synthetic mRNA Using Hollow Microneedles for Efficient and Rapid Production of Exogenous Proteins in Skin
    Article Snippet: The plasmid pEX-A2 containing the secretable hGLuc coding sequence was produced by Eurofins Genomics (Ebersberg, Germany). .. Briefly, the plasmid insert was amplified by PCR using the HotStar HiFidelity polymerase kit (QIAGEN, Hilden, Germany) and 0.7 μM of each forward (5′-TTGGACCCTCGTACAGAAGCTAATACG-3′) and reverse primer (5′-T120 -CTTCCTACTCAGGCTTTATTCAAAGACCA-3′).

    Article Title: Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing
    Article Snippet: .. Master mixes for PCR reactions contained the Qiagen Hotstar Hi-Fidelity Polymerase Kit (Qiagen, Valencia CA) with a forward primer composed of the Roche Titanium Fusion Primer A (5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’), a 10bp Multiplex Identifier (MID) sequence (Roche, Indianapolis, IN), unique to each sample, and the universal bacterial primer 8F (5'-AGAGTTTGATCCTGGCTCAG-3') ( ). .. The reverse primer was composed of the Roche Titanium Primer B (5’-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG -3’), the identical 10bp MID sequence as the forward primer, and the reverse bacterial primer 338R (5’-GCTGCCTCCCGTAGGAGT-3’) ( ).

    Article Title: Temporal changes in the bacterial community of animal feces and their correlation with stable fly oviposition, larval development, and adult fitness
    Article Snippet: The bacterial tag-encoded FLX-Titanium 16S rDNA amplicon parallel pyrosequencing and post sequencing processing were carried out at the Medical Biofilm Research Institute (Lubbock, TX, USA) as described by and . .. A single step reaction with 30 cycles was used and 0.5 U of HotStar HiFidelity Polymerase (Qiagen Inc., Valencia, CA, USA) was added to each reaction.

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: Analysis of allelic expression Expression from each X chromosome was verified using polymorphisms within the coding region and/or the UTRs of Huwe1 , Arhgef6 and Nsbp1 by direct sequencing and, whenever possible, by single-base primer-extension (SNaPshot). .. Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer.

    Article Title: Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5
    Article Snippet: CD44s ORF was amplified from LN229 cDNA using HotStar HiFidelity Polymerase Kit (Qiagen Inc., Hilden, Germany). .. CD44v3-10 ORF and CD44s ORF were subcloned into pCAG-cRAP-MAP vector possessing C-terminal RAP/MAP tag and pCAG-ssPA16 vector possessing signal sequence and N-terminal PA16 tag (GLEGGVAMPGAEDDVV), respectively.

    Article Title: HCV Genome-Wide Genetic Analyses in Context of Disease Progression and Hepatocellular Carcinoma
    Article Snippet: Paragraph title: Clonal sequencing in the E2 gene ... HCV sequences were amplified by nested PCR from the cDNAs under high-fidelity conditions employing the Hotstart HiFidelity Polymerase kit (Qiagen).

    Article Title: DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region
    Article Snippet: .. NGS Methods Barcode region amplifications were conducted using Hotstar Hifidelity polymerase (Qiagen) as per manufacturer’s instructions, and barcode primers with NGS priming site attachments as follows: ITS1 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGKARAAGTCGTAACAAGG-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGTTCAAAGAYTCGATGRTTC-3′ ITS2 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCTTCTCCAGACTACAAT-3′ After initial barcode region amplification, PCR products were purified (AMPure, Agencourt) and pooled, and a library constructed using these based on the Illumina 16S Metagenomic Sequencing Library Preparation. .. Index PCR with Nextera XT Index Kit v2 SetC (Illumina, San Diego, CA, USA) was conducted using KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) as per manufacturer’s instructions.

    Binding Assay:

    Article Title: DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region
    Article Snippet: NGS Methods Barcode region amplifications were conducted using Hotstar Hifidelity polymerase (Qiagen) as per manufacturer’s instructions, and barcode primers with NGS priming site attachments as follows: ITS1 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGKARAAGTCGTAACAAGG-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGTTCAAAGAYTCGATGRTTC-3′ ITS2 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCTTCTCCAGACTACAAT-3′ After initial barcode region amplification, PCR products were purified (AMPure, Agencourt) and pooled, and a library constructed using these based on the Illumina 16S Metagenomic Sequencing Library Preparation. .. The PCR assays were cleaned using AMPure beads following the published protocol, with the following modifications: 1:1 PCR product: AMPure bead initial binding; elution in 10 mM Tris pH 8.0.

    Molecular Cloning:

    Article Title: LIV-1 ZIP Ectodomain Shedding in Prion-Infected Mice Resembles Cellular Response to Transition Metal Starvation
    Article Snippet: Paragraph title: Molecular cloning ... The HotStar HiFidelity polymerase (Qiagen, Valencia, CA, USA) was used for site-directed mutagenesis.

    Mutagenesis:

    Article Title: LIV-1 ZIP Ectodomain Shedding in Prion-Infected Mice Resembles Cellular Response to Transition Metal Starvation
    Article Snippet: .. The HotStar HiFidelity polymerase (Qiagen, Valencia, CA, USA) was used for site-directed mutagenesis. .. A KLH-conjugated peptide derived from mouse ZIP10 (CNHDHSEQYEHNR) was synthesized and injected into Charles River Laboratories SPF rabbits (Division of Comparative Medicine, University of Toronto).

    Isolation:

    Article Title: HCV Genome-Wide Genetic Analyses in Context of Disease Progression and Hepatocellular Carcinoma
    Article Snippet: HCV RNAs were isolated and cDNA was synthesized as was done for the direct sequencing. .. HCV sequences were amplified by nested PCR from the cDNAs under high-fidelity conditions employing the Hotstart HiFidelity Polymerase kit (Qiagen).

    Article Title: Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis
    Article Snippet: Chromosomal DNAs of C. jejuni 81–176 and L. lactis used for PCR reactions were isolated using a commercial kit and protocol (A & A Biotechnology, Poland). .. Polymerase chain reactions (PCRs) were performed with PrimeStar HS DNA Polymerase (TaKaRa) or HotStar HiFidelity Polymerase (Qiagen) under standard conditions.

    Size-exclusion Chromatography:

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer. .. PCR conditions were the following: 15 min pre-incubation step at 94°C, 35 cycles of denaturation at 94°C for 30 sec, annealing for 45 sec at the respective AT for each primer pair and extension at 72°C for 45 sec, followed by a final extension step at 72°C for 10 min.

    Purification:

    Article Title: Intradermal Delivery of Synthetic mRNA Using Hollow Microneedles for Efficient and Rapid Production of Exogenous Proteins in Skin
    Article Snippet: Briefly, the plasmid insert was amplified by PCR using the HotStar HiFidelity polymerase kit (QIAGEN, Hilden, Germany) and 0.7 μM of each forward (5′-TTGGACCCTCGTACAGAAGCTAATACG-3′) and reverse primer (5′-T120 -CTTCCTACTCAGGCTTTATTCAAAGACCA-3′). .. The PCR product was purified using the MinElute PCR purification kit (QIAGEN) according manufacturer’s instructions.

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer. .. The PCR products were then purified with ExoSAP-IT (Usb) and single-base primer-extension reactions were carried out with SNaPshot Multiplex Kit (Applied Biosystems) in a 5 μl volume comprising 0.2 μM of the extension primer and up to 2 μl of the purified PCR product (depending on the expression level of each gene in the different tissues tested), for 25 cycles.

    Article Title: The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target) The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target
    Article Snippet: PCRs were performed using HotStar HiFidelity polymerase kit (Qiagen) in a Perkin‐ MJ Research PTC‐200 Peltier Thermal Cycler. .. PCR products and restriction digested DNA were purified using the PCR Mini Elute kit (Qiagen).

    Article Title: DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region
    Article Snippet: .. NGS Methods Barcode region amplifications were conducted using Hotstar Hifidelity polymerase (Qiagen) as per manufacturer’s instructions, and barcode primers with NGS priming site attachments as follows: ITS1 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGKARAAGTCGTAACAAGG-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGTTCAAAGAYTCGATGRTTC-3′ ITS2 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCTTCTCCAGACTACAAT-3′ After initial barcode region amplification, PCR products were purified (AMPure, Agencourt) and pooled, and a library constructed using these based on the Illumina 16S Metagenomic Sequencing Library Preparation. .. Index PCR with Nextera XT Index Kit v2 SetC (Illumina, San Diego, CA, USA) was conducted using KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) as per manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: .. The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen). ..

    Article Title: Generation and validation of homozygous fluorescent knock-in cells using CRISPR/Cas9 genome editing
    Article Snippet: .. Oligos for amplification of target sequence and sequencing (see ) HotStar HighFidelity PCR kit (QIAGEN, cat. no. 202605) MinElute Gel Extraction Kit (QIAGEN, cat. no. 28604) .. Cas9-HRP antibody (abcam, cat. no. ab202580) GFP antibody (Roche, cat. no. 11814460001) RFP antibody [3F5] (ChromoTek, cat. no. 3f5) Non-Fat Dry Milk powder DTT (Merck, cat. D0632) cOmplete EDTA-free Protease Inhibitor Tablets (Roche, cat. no.11873580001) PhosStop Tablets (Roche, cat.no.

    Article Title: Intradermal Delivery of Synthetic mRNA Using Hollow Microneedles for Efficient and Rapid Production of Exogenous Proteins in Skin
    Article Snippet: .. Briefly, the plasmid insert was amplified by PCR using the HotStar HiFidelity polymerase kit (QIAGEN, Hilden, Germany) and 0.7 μM of each forward (5′-TTGGACCCTCGTACAGAAGCTAATACG-3′) and reverse primer (5′-T120 -CTTCCTACTCAGGCTTTATTCAAAGACCA-3′). .. Primers were purchased from ELLA Biotech (Martinsried, Germany) in high-performance liquid chromatography (HPLC) grade.

    Article Title: Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing
    Article Snippet: .. Master mixes for PCR reactions contained the Qiagen Hotstar Hi-Fidelity Polymerase Kit (Qiagen, Valencia CA) with a forward primer composed of the Roche Titanium Fusion Primer A (5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’), a 10bp Multiplex Identifier (MID) sequence (Roche, Indianapolis, IN), unique to each sample, and the universal bacterial primer 8F (5'-AGAGTTTGATCCTGGCTCAG-3') ( ). .. The reverse primer was composed of the Roche Titanium Primer B (5’-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG -3’), the identical 10bp MID sequence as the forward primer, and the reverse bacterial primer 338R (5’-GCTGCCTCCCGTAGGAGT-3’) ( ).

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer. .. PCR conditions were the following: 15 min pre-incubation step at 94°C, 35 cycles of denaturation at 94°C for 30 sec, annealing for 45 sec at the respective AT for each primer pair and extension at 72°C for 45 sec, followed by a final extension step at 72°C for 10 min.

    Article Title: The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target) The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target
    Article Snippet: PCRs were performed using HotStar HiFidelity polymerase kit (Qiagen) in a Perkin‐ MJ Research PTC‐200 Peltier Thermal Cycler. .. PCR products and restriction digested DNA were purified using the PCR Mini Elute kit (Qiagen).

    Article Title: DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region
    Article Snippet: .. NGS Methods Barcode region amplifications were conducted using Hotstar Hifidelity polymerase (Qiagen) as per manufacturer’s instructions, and barcode primers with NGS priming site attachments as follows: ITS1 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGKARAAGTCGTAACAAGG-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGTTCAAAGAYTCGATGRTTC-3′ ITS2 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCTTCTCCAGACTACAAT-3′ After initial barcode region amplification, PCR products were purified (AMPure, Agencourt) and pooled, and a library constructed using these based on the Illumina 16S Metagenomic Sequencing Library Preparation. .. Index PCR with Nextera XT Index Kit v2 SetC (Illumina, San Diego, CA, USA) was conducted using KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) as per manufacturer’s instructions.

    Article Title: Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis
    Article Snippet: Chromosomal DNAs of C. jejuni 81–176 and L. lactis used for PCR reactions were isolated using a commercial kit and protocol (A & A Biotechnology, Poland). .. Polymerase chain reactions (PCRs) were performed with PrimeStar HS DNA Polymerase (TaKaRa) or HotStar HiFidelity Polymerase (Qiagen) under standard conditions.

    Staining:

    Article Title: The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target) The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target
    Article Snippet: DNA samples were analysed by agarose gel electrophoresis and visualised by staining with SYBR Safe (Invitrogen). .. PCRs were performed using HotStar HiFidelity polymerase kit (Qiagen) in a Perkin‐ MJ Research PTC‐200 Peltier Thermal Cycler.

    Nested PCR:

    Article Title: HCV Genome-Wide Genetic Analyses in Context of Disease Progression and Hepatocellular Carcinoma
    Article Snippet: .. HCV sequences were amplified by nested PCR from the cDNAs under high-fidelity conditions employing the Hotstart HiFidelity Polymerase kit (Qiagen). ..

    Chromatin Immunoprecipitation:

    Article Title: DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region
    Article Snippet: NGS Methods Barcode region amplifications were conducted using Hotstar Hifidelity polymerase (Qiagen) as per manufacturer’s instructions, and barcode primers with NGS priming site attachments as follows: ITS1 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGKARAAGTCGTAACAAGG-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGTTCAAAGAYTCGATGRTTC-3′ ITS2 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCTTCTCCAGACTACAAT-3′ After initial barcode region amplification, PCR products were purified (AMPure, Agencourt) and pooled, and a library constructed using these based on the Illumina 16S Metagenomic Sequencing Library Preparation. .. The resulting libraries were quality controlled using SpectraMax Quickdrop, Qubit HS dsDNA kit and were then run on Bioanalyzer with a HS DNA chip, after being diluted to approximately 1 ng/µl.

    Plasmid Preparation:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: Paragraph title: 1.1. Bacterial expression plasmid construction ... The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen).

    Article Title: LIV-1 ZIP Ectodomain Shedding in Prion-Infected Mice Resembles Cellular Response to Transition Metal Starvation
    Article Snippet: ZIP10-HA_d300 (deleted from residue 31 to residue 300), ZIP10-HA_d389 (deleted from residue 36 to residue 389), ZIP10-HA_N341A and ZIP10-HA-(H711A/H715A) were derived from the ZIP10-HA plasmid. .. The HotStar HiFidelity polymerase (Qiagen, Valencia, CA, USA) was used for site-directed mutagenesis.

    Article Title: Intradermal Delivery of Synthetic mRNA Using Hollow Microneedles for Efficient and Rapid Production of Exogenous Proteins in Skin
    Article Snippet: .. Briefly, the plasmid insert was amplified by PCR using the HotStar HiFidelity polymerase kit (QIAGEN, Hilden, Germany) and 0.7 μM of each forward (5′-TTGGACCCTCGTACAGAAGCTAATACG-3′) and reverse primer (5′-T120 -CTTCCTACTCAGGCTTTATTCAAAGACCA-3′). .. Primers were purchased from ELLA Biotech (Martinsried, Germany) in high-performance liquid chromatography (HPLC) grade.

    Article Title: The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target) The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target
    Article Snippet: Genomic DNA was extracted from N. meningitidis using the Gentra Puregene Yeast/Bact Kit (Qiagen) and plasmid DNA was extracted from E. coli using the QiaPrep Spin kit (Qiagen). .. PCRs were performed using HotStar HiFidelity polymerase kit (Qiagen) in a Perkin‐ MJ Research PTC‐200 Peltier Thermal Cycler.

    Article Title: Detection of high CD44 expression in oral cancers using the novel monoclonal antibody, C44Mab-5
    Article Snippet: CD44s ORF was amplified from LN229 cDNA using HotStar HiFidelity Polymerase Kit (Qiagen Inc., Hilden, Germany). .. CD44v3-10 ORF and CD44s ORF were subcloned into pCAG-cRAP-MAP vector possessing C-terminal RAP/MAP tag and pCAG-ssPA16 vector possessing signal sequence and N-terminal PA16 tag (GLEGGVAMPGAEDDVV), respectively.

    Software:

    Article Title: Temporal changes in the bacterial community of animal feces and their correlation with stable fly oviposition, larval development, and adult fitness
    Article Snippet: A single step reaction with 30 cycles was used and 0.5 U of HotStar HiFidelity Polymerase (Qiagen Inc., Valencia, CA, USA) was added to each reaction. .. Sequence collections were depleted of chimeras using B2C2 software ( ).

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer. .. SNaPshot reactions were cleaned up with Shrimp Alkaline Phosphatase (Usb) and the analysis of fluorescent products was performed in an ABI 3100 sequencer using the GeneMapper 4.0 Analysis Software.

    Multiplex Assay:

    Article Title: Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing
    Article Snippet: .. Master mixes for PCR reactions contained the Qiagen Hotstar Hi-Fidelity Polymerase Kit (Qiagen, Valencia CA) with a forward primer composed of the Roche Titanium Fusion Primer A (5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’), a 10bp Multiplex Identifier (MID) sequence (Roche, Indianapolis, IN), unique to each sample, and the universal bacterial primer 8F (5'-AGAGTTTGATCCTGGCTCAG-3') ( ). .. The reverse primer was composed of the Roche Titanium Primer B (5’-CCTATCCCCTGTGTGCCTTGGCAGTCTCAG -3’), the identical 10bp MID sequence as the forward primer, and the reverse bacterial primer 338R (5’-GCTGCCTCCCGTAGGAGT-3’) ( ).

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer. .. The PCR products were then purified with ExoSAP-IT (Usb) and single-base primer-extension reactions were carried out with SNaPshot Multiplex Kit (Applied Biosystems) in a 5 μl volume comprising 0.2 μM of the extension primer and up to 2 μl of the purified PCR product (depending on the expression level of each gene in the different tissues tested), for 25 cycles.

    Agarose Gel Electrophoresis:

    Article Title: The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target) The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target
    Article Snippet: DNA samples were analysed by agarose gel electrophoresis and visualised by staining with SYBR Safe (Invitrogen). .. PCRs were performed using HotStar HiFidelity polymerase kit (Qiagen) in a Perkin‐ MJ Research PTC‐200 Peltier Thermal Cycler.

    Next-Generation Sequencing:

    Article Title: DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region
    Article Snippet: .. NGS Methods Barcode region amplifications were conducted using Hotstar Hifidelity polymerase (Qiagen) as per manufacturer’s instructions, and barcode primers with NGS priming site attachments as follows: ITS1 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGAAGKARAAGTCGTAACAAGG-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCGTTCAAAGAYTCGATGRTTC-3′ ITS2 region: Forward: 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATGCGATACTTGGTGTGAAT-3′ Reverse: 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACGCTTCTCCAGACTACAAT-3′ After initial barcode region amplification, PCR products were purified (AMPure, Agencourt) and pooled, and a library constructed using these based on the Illumina 16S Metagenomic Sequencing Library Preparation. .. Index PCR with Nextera XT Index Kit v2 SetC (Illumina, San Diego, CA, USA) was conducted using KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) as per manufacturer’s instructions.

    Produced:

    Article Title: Intradermal Delivery of Synthetic mRNA Using Hollow Microneedles for Efficient and Rapid Production of Exogenous Proteins in Skin
    Article Snippet: The plasmid pEX-A2 containing the secretable hGLuc coding sequence was produced by Eurofins Genomics (Ebersberg, Germany). .. Briefly, the plasmid insert was amplified by PCR using the HotStar HiFidelity polymerase kit (QIAGEN, Hilden, Germany) and 0.7 μM of each forward (5′-TTGGACCCTCGTACAGAAGCTAATACG-3′) and reverse primer (5′-T120 -CTTCCTACTCAGGCTTTATTCAAAGACCA-3′).

    Oligonucleotide Synthesis:

    Article Title: Cell Wall Anchoring of the Campylobacter Antigens to Lactococcus lactis
    Article Snippet: Polymerase chain reactions (PCRs) were performed with PrimeStar HS DNA Polymerase (TaKaRa) or HotStar HiFidelity Polymerase (Qiagen) under standard conditions. .. Synthetic oligonucleotide synthesis and DNA sequencing for cloning experiments were performed by Genomed S.A., Warsaw.

    Gel Extraction:

    Article Title: Generation and validation of homozygous fluorescent knock-in cells using CRISPR/Cas9 genome editing
    Article Snippet: .. Oligos for amplification of target sequence and sequencing (see ) HotStar HighFidelity PCR kit (QIAGEN, cat. no. 202605) MinElute Gel Extraction Kit (QIAGEN, cat. no. 28604) .. Cas9-HRP antibody (abcam, cat. no. ab202580) GFP antibody (Roche, cat. no. 11814460001) RFP antibody [3F5] (ChromoTek, cat. no. 3f5) Non-Fat Dry Milk powder DTT (Merck, cat. D0632) cOmplete EDTA-free Protease Inhibitor Tablets (Roche, cat. no.11873580001) PhosStop Tablets (Roche, cat.no.

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