hotstar taq plus master mix kit  (Qiagen)


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    Name:
    HotStarTaq Plus Master Mix Kit
    Description:
    For fast and highly specific amplification in all applications Kit contents Qiagen HotStarTaq Plus Master Mix Kit 250 x 20μL rxns 5U L Genomic DNA and cDNA Sample 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate PCR Amplification Reaction Type Ideal for PCR RT PCR Includes 3 x 0 85mL HotStarTaq Plus Master Mix Contains 250U of HotStarTaq Plus DNA Polymerase PCR Buffer with 3mM MgCl2 and 400μM of Each dNT 1 x 0 55mL CoralLoad Concentrate 2 x 1 9mL RNase free Water Benefits Fast 5 minute enzyme activation time Fewer pipetting steps reduces the risk of contamination High PCR specificity without the need for optimization Optional ready to load buffer additive for easier handling
    Catalog Number:
    203643
    Price:
    194
    Category:
    HotStarTaq Plus Master Mix Kit
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    Structured Review

    Qiagen hotstar taq plus master mix kit
    HotStarTaq Plus Master Mix Kit
    For fast and highly specific amplification in all applications Kit contents Qiagen HotStarTaq Plus Master Mix Kit 250 x 20μL rxns 5U L Genomic DNA and cDNA Sample 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate PCR Amplification Reaction Type Ideal for PCR RT PCR Includes 3 x 0 85mL HotStarTaq Plus Master Mix Contains 250U of HotStarTaq Plus DNA Polymerase PCR Buffer with 3mM MgCl2 and 400μM of Each dNT 1 x 0 55mL CoralLoad Concentrate 2 x 1 9mL RNase free Water Benefits Fast 5 minute enzyme activation time Fewer pipetting steps reduces the risk of contamination High PCR specificity without the need for optimization Optional ready to load buffer additive for easier handling
    https://www.bioz.com/result/hotstar taq plus master mix kit/product/Qiagen
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    hotstar taq plus master mix kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: Study on the association of helicobacter species with viral hepatitis-induced hepatocellular carcinoma
    Article Snippet: The integrity of the extracted DNA was confirmed by performing a “universal bacterial PCR” (as used in our diagnostic routine laboratory) using the forward primer “PF1” (5'-AGA GTT TGA TCC TGG CTC AG-3') and the reverse primer “UniNadR” (5'-GGA CTA CCA GGG TAT CTA ATC CTG TT-3'). .. PCRs were performed using the “HotStarTaq Plus Mastermix kit” from Qiagen in a final volume of 25 ∝l, final concentration of primers was 1 ∝M.

    Clone Assay:

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: Paragraph title: 2.5. Isolation and cloning of single cell immunoglobulin V(D)J rearrangements from vaccine-induced plasmablasts ... The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Centrifugation:

    Article Title: Culex quinquefasciatus larval microbiomes vary with instar and exposure to common wastewater contaminants
    Article Snippet: In addition to mosquitoes samples of water and water plus diet were also extracted using identical protocols, with the additional step of centrifugation at 2900 rpm in an IEC HN-SII tabletop centrifuge for 1 h to create a pellet. .. The procedure used the forward primer 27Fmod (GRGTTTGATCMTGGCTCAG) and the reverse primer 519Rmodbio (GTNTTACNGCGGCKGCTG) in a single-step 30 cycle PCR using HotStarTaq Plus Master Mix (Qiagen, Valencia, CA).

    Amplification:

    Article Title: Human mining activity across the ages determines the genetic structure of modern brown trout (Salmo trutta L.) populations
    Article Snippet: .. PCR amplification was carried out using a BIO-RAD MyCycler Thermal Cycler in 10 μ L reaction volumes containing 1 μ L of extracted DNA (c. 30 ng DNA), 3 μ L RNase-free water, 5 μ L of QIAGEN HotStarTaq Plus Master Mix, and 1 μ L of primer mixture, in a total of 8 microsatellite multiplexes ( ). .. PCR conditions were as follows: an initial denaturing step at 95°C for 5 min was followed by 20 cycles of touchdown PCR consisting of 30 s at 94°C, a 30 s annealing step starting at 60°C or 55°C and decreasing by 0.5°C each cycle until a touchdown temperature of 50°C or 45°C (dependent on multiplex; ) was achieved, and an elongation step of 72°C for 30 s, followed by 15 cycles comprising 94°C for 30 s, 50°C or 45°C for 30 s, and 72°C for 30 s. This was followed by a final 10 min extension step at 72°C.

    Article Title: Culex quinquefasciatus larval microbiomes vary with instar and exposure to common wastewater contaminants
    Article Snippet: Roche 454 bacteria barcoded amplicon pyrosequencing was performed by a commercial sequencing facility (Molecular Research LP MR DNA, Shallowater, TX). .. The procedure used the forward primer 27Fmod (GRGTTTGATCMTGGCTCAG) and the reverse primer 519Rmodbio (GTNTTACNGCGGCKGCTG) in a single-step 30 cycle PCR using HotStarTaq Plus Master Mix (Qiagen, Valencia, CA).

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: Paragraph title: 3. PCR Amplification ... Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR.

    Article Title: Diversity of Meq gene from clinical Marek’s disease virus infection in Saudi Arabia
    Article Snippet: Detection of the Meq oncoprotein gene The extracted DNAs were screened for presence of MDV using HotStartTaq® Plus Master Mix Kit (QIAGEN, USA). .. 2 µl sample of each purified genomic DNAs was amplified in 20 µl of the final volume of a 2X HotStartTaq Plus Master Mix containing 1.5 mM MgCl2 , 200 µM of each dNTP, 1 unit HotStartTaq Plus DNA polymerase, and 10 µM of each forward (F:GCACTCTAGAGTGTA AAGAGATGTC TCAG) and reverse (R:TAACTCG AGGAGAAGAAACATGG GGCATAG) primers [ ].

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: Using a multiplex nested PCR, either IgG, IgA or IgM heavy chain and kappa or lambda immunoglobulin (Ig) light chain fragments were amplified from the cDNA using primers recently described ( ). .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Article Title: The Composition of Midgut Bacteria in Aedes aegypti (Diptera: Culicidae) That Are Naturally Susceptible or Refractory to Dengue Viruses
    Article Snippet: Paragraph title: Amplicon Library Preparation and Sequencing ... The PCR was performed using the HotStartTaq Plus Master Mix Kit (Qiagen, Hilden, Germany) under the following conditions: 94°C for 3 min, 28 cycles of 94°C for 30 s, 53°C for 40 s, and 72°C for 1 min, after which a final elongation step at 72°C for 5 min was performed.

    Synthesized:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: Extracted RNA was used to generate cDNA and subsequently for conventional RT-PCR and qRT-PCR. cDNA was synthesized from 300 ng of purified total RNA, 160 ng random primers, 10 ng oligo(dT)12–18 , 0.5 mM dNTPs, 10 mM DTT, 2 U RNase inhibitor, and 400 U Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). .. PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA).

    Quantitative RT-PCR:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: Extracted RNA was used to generate cDNA and subsequently for conventional RT-PCR and qRT-PCR. cDNA was synthesized from 300 ng of purified total RNA, 160 ng random primers, 10 ng oligo(dT)12–18 , 0.5 mM dNTPs, 10 mM DTT, 2 U RNase inhibitor, and 400 U Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). .. PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA).

    Electrophoresis:

    Article Title: Macacine Herpesvirus 1 in Long-Tailed Macaques, Malaysia, 2009–2011
    Article Snippet: Briefly, MaHV1 primers (B virus 1, 5′-ACCTCACGTACGACTCCGACT-3′; and B virus 2, 5′-CTGCAGGACCGAGTAGAGGAT-3′; 2.5 μmol/L) were each added to the extraction product and HotStarTaq Plus Master Mix (QIAGEN, Hilden, Germany). .. The products (10 μL) were then analyzed by electrophoresis on 1% agarose gels.

    Incubation:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA). .. Hot start reactions were initiated by a 5-min incubation at 95°C and amplifications were performed utilizing 35 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 1 min. Transcript expression was detected using 5 μl of PCR product on a 2% agarose gel.

    Expressing:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: Conventional PCR was used to validate PCR primers and screen transcript expression of various genes. .. PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA).

    Touchdown PCR:

    Article Title: Human mining activity across the ages determines the genetic structure of modern brown trout (Salmo trutta L.) populations
    Article Snippet: PCR amplification was carried out using a BIO-RAD MyCycler Thermal Cycler in 10 μ L reaction volumes containing 1 μ L of extracted DNA (c. 30 ng DNA), 3 μ L RNase-free water, 5 μ L of QIAGEN HotStarTaq Plus Master Mix, and 1 μ L of primer mixture, in a total of 8 microsatellite multiplexes ( ). .. PCR conditions were as follows: an initial denaturing step at 95°C for 5 min was followed by 20 cycles of touchdown PCR consisting of 30 s at 94°C, a 30 s annealing step starting at 60°C or 55°C and decreasing by 0.5°C each cycle until a touchdown temperature of 50°C or 45°C (dependent on multiplex; ) was achieved, and an elongation step of 72°C for 30 s, followed by 15 cycles comprising 94°C for 30 s, 50°C or 45°C for 30 s, and 72°C for 30 s. This was followed by a final 10 min extension step at 72°C.

    Modification:

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR. .. Top DNA polymerase, used in the AccuPower PCR PreMix, was a modified form of a recombinant DNA polymerase, originally isolated from Thermus thermophilus .

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: .. The AmpliTaq Gold DNA Polymerase in the AmpliTaq Gold 360 Master Mix, and the HotStarTaq Plus DNA Polymerase in the HotStarTaq Plus Master Mix (Qiagen) were recombinant forms modified from the Thermus aquaticus (Taq ) DNA polymerase. .. Aliquots of the UCNPs solutions were added to the PCR mixtures to achieve appropriate concentrations.

    Transformation Assay:

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: The entire antibody cloning process was divided in various sequential steps: cDNA production, nested PCR, sequencing of nested PCR products (search for cloning primers), cloning PCR, plasmid construction, bacteria transformation, plasmid sequencing (search for open reading frames (ORFs)), 293A cell transfection and mAb purification as previously described ( ; ; ) with some modifications. .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Transfection:

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: The entire antibody cloning process was divided in various sequential steps: cDNA production, nested PCR, sequencing of nested PCR products (search for cloning primers), cloning PCR, plasmid construction, bacteria transformation, plasmid sequencing (search for open reading frames (ORFs)), 293A cell transfection and mAb purification as previously described ( ; ; ) with some modifications. .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Concentration Assay:

    Article Title: Study on the association of helicobacter species with viral hepatitis-induced hepatocellular carcinoma
    Article Snippet: .. PCRs were performed using the “HotStarTaq Plus Mastermix kit” from Qiagen in a final volume of 25 ∝l, final concentration of primers was 1 ∝M. .. PCR was performed with a GeneAmp PCR System 9700 Thermocycler (Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: Paragraph title: Whole-tissue and single-cell RT-PCR ... PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA).

    other:

    Article Title: Fate Tracing of neurogenin2-Expressing Cells in the Mouse Retina Using CreER™: LacZ
    Article Snippet: HotStartTaq Plus Master Mix Kit (1000) (Qiagen) (see ).

    Sequencing:

    Article Title: Culex quinquefasciatus larval microbiomes vary with instar and exposure to common wastewater contaminants
    Article Snippet: Roche 454 bacteria barcoded amplicon pyrosequencing was performed by a commercial sequencing facility (Molecular Research LP MR DNA, Shallowater, TX). .. The procedure used the forward primer 27Fmod (GRGTTTGATCMTGGCTCAG) and the reverse primer 519Rmodbio (GTNTTACNGCGGCKGCTG) in a single-step 30 cycle PCR using HotStarTaq Plus Master Mix (Qiagen, Valencia, CA).

    Article Title: Feline panleukopenia virus in cerebral neurons of young and adult cats
    Article Snippet: Paragraph title: Next-generation sequencing, confirmation PCRs and sequence analysis ... PCRs were performed using HotStarTaq Plus Master Mix (Qiagen, Hilden, Germany) and conditions were as follows: a denaturation step at 95 °C for 5 min, followed by 45 cycles of 95 °C for 20 s, 55 °C for 45 s, 72 °C for 2 min, and final elongation 5 min at 72 °C.

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: The entire antibody cloning process was divided in various sequential steps: cDNA production, nested PCR, sequencing of nested PCR products (search for cloning primers), cloning PCR, plasmid construction, bacteria transformation, plasmid sequencing (search for open reading frames (ORFs)), 293A cell transfection and mAb purification as previously described ( ; ; ) with some modifications. .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Article Title: The Composition of Midgut Bacteria in Aedes aegypti (Diptera: Culicidae) That Are Naturally Susceptible or Refractory to Dengue Viruses
    Article Snippet: Paragraph title: Amplicon Library Preparation and Sequencing ... The PCR was performed using the HotStartTaq Plus Master Mix Kit (Qiagen, Hilden, Germany) under the following conditions: 94°C for 3 min, 28 cycles of 94°C for 30 s, 53°C for 40 s, and 72°C for 1 min, after which a final elongation step at 72°C for 5 min was performed.

    Recombinant:

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR. .. Top DNA polymerase, used in the AccuPower PCR PreMix, was a modified form of a recombinant DNA polymerase, originally isolated from Thermus thermophilus .

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: .. The AmpliTaq Gold DNA Polymerase in the AmpliTaq Gold 360 Master Mix, and the HotStarTaq Plus DNA Polymerase in the HotStarTaq Plus Master Mix (Qiagen) were recombinant forms modified from the Thermus aquaticus (Taq ) DNA polymerase. .. Aliquots of the UCNPs solutions were added to the PCR mixtures to achieve appropriate concentrations.

    DNA Extraction:

    Article Title: Human mining activity across the ages determines the genetic structure of modern brown trout (Salmo trutta L.) populations
    Article Snippet: Paragraph title: DNA extraction, PCR, and genotyping ... PCR amplification was carried out using a BIO-RAD MyCycler Thermal Cycler in 10 μ L reaction volumes containing 1 μ L of extracted DNA (c. 30 ng DNA), 3 μ L RNase-free water, 5 μ L of QIAGEN HotStarTaq Plus Master Mix, and 1 μ L of primer mixture, in a total of 8 microsatellite multiplexes ( ).

    Article Title: Study on the association of helicobacter species with viral hepatitis-induced hepatocellular carcinoma
    Article Snippet: Paragraph title: DNA extraction and PCR assays. ... PCRs were performed using the “HotStarTaq Plus Mastermix kit” from Qiagen in a final volume of 25 ∝l, final concentration of primers was 1 ∝M.

    Mutagenesis:

    Article Title: Feline panleukopenia virus in cerebral neurons of young and adult cats
    Article Snippet: A confirmation PCR was used to verify a point mutation in NS1 coding sequence. .. PCRs were performed using HotStarTaq Plus Master Mix (Qiagen, Hilden, Germany) and conditions were as follows: a denaturation step at 95 °C for 5 min, followed by 45 cycles of 95 °C for 20 s, 55 °C for 45 s, 72 °C for 2 min, and final elongation 5 min at 72 °C.

    Isolation:

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR. .. Top DNA polymerase, used in the AccuPower PCR PreMix, was a modified form of a recombinant DNA polymerase, originally isolated from Thermus thermophilus .

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: Paragraph title: 2.5. Isolation and cloning of single cell immunoglobulin V(D)J rearrangements from vaccine-induced plasmablasts ... The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Purification:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: Extracted RNA was used to generate cDNA and subsequently for conventional RT-PCR and qRT-PCR. cDNA was synthesized from 300 ng of purified total RNA, 160 ng random primers, 10 ng oligo(dT)12–18 , 0.5 mM dNTPs, 10 mM DTT, 2 U RNase inhibitor, and 400 U Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). .. PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA).

    Article Title: Macacine Herpesvirus 1 in Long-Tailed Macaques, Malaysia, 2009–2011
    Article Snippet: Briefly, MaHV1 primers (B virus 1, 5′-ACCTCACGTACGACTCCGACT-3′; and B virus 2, 5′-CTGCAGGACCGAGTAGAGGAT-3′; 2.5 μmol/L) were each added to the extraction product and HotStarTaq Plus Master Mix (QIAGEN, Hilden, Germany). .. Of the ≈10% of samples that were positive by PCR, 14 PCR products were randomly selected and purified with a PCR purification kit (QIAGEN) and sequenced by using the same primers to confirm identity.

    Article Title: Culex quinquefasciatus larval microbiomes vary with instar and exposure to common wastewater contaminants
    Article Snippet: The procedure used the forward primer 27Fmod (GRGTTTGATCMTGGCTCAG) and the reverse primer 519Rmodbio (GTNTTACNGCGGCKGCTG) in a single-step 30 cycle PCR using HotStarTaq Plus Master Mix (Qiagen, Valencia, CA). .. PCR was performed using the following cycle conditions: 94 °C for 3 min, followed by 28 cycles of 94 °C for 30 s; 53 °C for 40 s, 72 °C for 1 min; and a final elongation at 72 °C for 5 min. After PCR, all amplicons were mixed in equal concentrations and purified using Agencourt Ampure beads (Agencourt Bioscience Corporation, MA).

    Article Title: Diversity of Meq gene from clinical Marek’s disease virus infection in Saudi Arabia
    Article Snippet: Detection of the Meq oncoprotein gene The extracted DNAs were screened for presence of MDV using HotStartTaq® Plus Master Mix Kit (QIAGEN, USA). .. 2 µl sample of each purified genomic DNAs was amplified in 20 µl of the final volume of a 2X HotStartTaq Plus Master Mix containing 1.5 mM MgCl2 , 200 µM of each dNTP, 1 unit HotStartTaq Plus DNA polymerase, and 10 µM of each forward (F:GCACTCTAGAGTGTA AAGAGATGTC TCAG) and reverse (R:TAACTCG AGGAGAAGAAACATGG GGCATAG) primers [ ].

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: The entire antibody cloning process was divided in various sequential steps: cDNA production, nested PCR, sequencing of nested PCR products (search for cloning primers), cloning PCR, plasmid construction, bacteria transformation, plasmid sequencing (search for open reading frames (ORFs)), 293A cell transfection and mAb purification as previously described ( ; ; ) with some modifications. .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Article Title: The Composition of Midgut Bacteria in Aedes aegypti (Diptera: Culicidae) That Are Naturally Susceptible or Refractory to Dengue Viruses
    Article Snippet: The PCR was performed using the HotStartTaq Plus Master Mix Kit (Qiagen, Hilden, Germany) under the following conditions: 94°C for 3 min, 28 cycles of 94°C for 30 s, 53°C for 40 s, and 72°C for 1 min, after which a final elongation step at 72°C for 5 min was performed. .. Samples were then purified using calibrated Agencourt AMPure XP beads (Brea, CA).

    Polymerase Chain Reaction:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: .. PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA). .. Hot start reactions were initiated by a 5-min incubation at 95°C and amplifications were performed utilizing 35 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 1 min. Transcript expression was detected using 5 μl of PCR product on a 2% agarose gel.

    Article Title: Macacine Herpesvirus 1 in Long-Tailed Macaques, Malaysia, 2009–2011
    Article Snippet: PCR was performed as previously described and validated by Scinicariello et al. ( ). .. Briefly, MaHV1 primers (B virus 1, 5′-ACCTCACGTACGACTCCGACT-3′; and B virus 2, 5′-CTGCAGGACCGAGTAGAGGAT-3′; 2.5 μmol/L) were each added to the extraction product and HotStarTaq Plus Master Mix (QIAGEN, Hilden, Germany).

    Article Title: Human mining activity across the ages determines the genetic structure of modern brown trout (Salmo trutta L.) populations
    Article Snippet: .. PCR amplification was carried out using a BIO-RAD MyCycler Thermal Cycler in 10 μ L reaction volumes containing 1 μ L of extracted DNA (c. 30 ng DNA), 3 μ L RNase-free water, 5 μ L of QIAGEN HotStarTaq Plus Master Mix, and 1 μ L of primer mixture, in a total of 8 microsatellite multiplexes ( ). .. PCR conditions were as follows: an initial denaturing step at 95°C for 5 min was followed by 20 cycles of touchdown PCR consisting of 30 s at 94°C, a 30 s annealing step starting at 60°C or 55°C and decreasing by 0.5°C each cycle until a touchdown temperature of 50°C or 45°C (dependent on multiplex; ) was achieved, and an elongation step of 72°C for 30 s, followed by 15 cycles comprising 94°C for 30 s, 50°C or 45°C for 30 s, and 72°C for 30 s. This was followed by a final 10 min extension step at 72°C.

    Article Title: Culex quinquefasciatus larval microbiomes vary with instar and exposure to common wastewater contaminants
    Article Snippet: .. The procedure used the forward primer 27Fmod (GRGTTTGATCMTGGCTCAG) and the reverse primer 519Rmodbio (GTNTTACNGCGGCKGCTG) in a single-step 30 cycle PCR using HotStarTaq Plus Master Mix (Qiagen, Valencia, CA). .. PCR was performed using the following cycle conditions: 94 °C for 3 min, followed by 28 cycles of 94 °C for 30 s; 53 °C for 40 s, 72 °C for 1 min; and a final elongation at 72 °C for 5 min. After PCR, all amplicons were mixed in equal concentrations and purified using Agencourt Ampure beads (Agencourt Bioscience Corporation, MA).

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: .. Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR. .. Top DNA polymerase, used in the AccuPower PCR PreMix, was a modified form of a recombinant DNA polymerase, originally isolated from Thermus thermophilus .

    Article Title: Study on the association of helicobacter species with viral hepatitis-induced hepatocellular carcinoma
    Article Snippet: Paragraph title: DNA extraction and PCR assays. ... PCRs were performed using the “HotStarTaq Plus Mastermix kit” from Qiagen in a final volume of 25 ∝l, final concentration of primers was 1 ∝M.

    Article Title: Feline panleukopenia virus in cerebral neurons of young and adult cats
    Article Snippet: A confirmation PCR was used to verify a point mutation in NS1 coding sequence. .. PCRs were performed using HotStarTaq Plus Master Mix (Qiagen, Hilden, Germany) and conditions were as follows: a denaturation step at 95 °C for 5 min, followed by 45 cycles of 95 °C for 20 s, 55 °C for 45 s, 72 °C for 2 min, and final elongation 5 min at 72 °C.

    Article Title: Diversity of Meq gene from clinical Marek’s disease virus infection in Saudi Arabia
    Article Snippet: Detection of the Meq oncoprotein gene The extracted DNAs were screened for presence of MDV using HotStartTaq® Plus Master Mix Kit (QIAGEN, USA). .. The amplified PCR products were electrophoresed in 1.5% agarose gel stained with ethidium bromide and documented using ultraviolet gel documentation system (BIORAD).

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well. .. The 2nd round PCR mix contained the same basic reagents as the first one, except for the MgCl2 and PCR primers.

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: Paragraph title: 3. PCR Amplification ... The AmpliTaq Gold DNA Polymerase in the AmpliTaq Gold 360 Master Mix, and the HotStarTaq Plus DNA Polymerase in the HotStarTaq Plus Master Mix (Qiagen) were recombinant forms modified from the Thermus aquaticus (Taq ) DNA polymerase.

    Article Title: The Composition of Midgut Bacteria in Aedes aegypti (Diptera: Culicidae) That Are Naturally Susceptible or Refractory to Dengue Viruses
    Article Snippet: .. The PCR was performed using the HotStartTaq Plus Master Mix Kit (Qiagen, Hilden, Germany) under the following conditions: 94°C for 3 min, 28 cycles of 94°C for 30 s, 53°C for 40 s, and 72°C for 1 min, after which a final elongation step at 72°C for 5 min was performed. ..

    Nested PCR:

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: Using a multiplex nested PCR, either IgG, IgA or IgM heavy chain and kappa or lambda immunoglobulin (Ig) light chain fragments were amplified from the cDNA using primers recently described ( ). .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Agarose Gel Electrophoresis:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA). .. Hot start reactions were initiated by a 5-min incubation at 95°C and amplifications were performed utilizing 35 cycles of 94°C for 30 s, 50°C for 30 s, and 72°C for 1 min. Transcript expression was detected using 5 μl of PCR product on a 2% agarose gel.

    Article Title: Diversity of Meq gene from clinical Marek’s disease virus infection in Saudi Arabia
    Article Snippet: Detection of the Meq oncoprotein gene The extracted DNAs were screened for presence of MDV using HotStartTaq® Plus Master Mix Kit (QIAGEN, USA). .. The amplified PCR products were electrophoresed in 1.5% agarose gel stained with ethidium bromide and documented using ultraviolet gel documentation system (BIORAD).

    Nucleic Acid Concentration:

    Article Title: Culex quinquefasciatus larval microbiomes vary with instar and exposure to common wastewater contaminants
    Article Snippet: Upon extraction, nucleic acid concentration was quantified using a Nanodrop ND- 2000c Spectrophotometer (Cole-Palmer, Vernon Hills, IL), to confirm enough genetic material for sequencing. .. The procedure used the forward primer 27Fmod (GRGTTTGATCMTGGCTCAG) and the reverse primer 519Rmodbio (GTNTTACNGCGGCKGCTG) in a single-step 30 cycle PCR using HotStarTaq Plus Master Mix (Qiagen, Valencia, CA).

    Plasmid Preparation:

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: The entire antibody cloning process was divided in various sequential steps: cDNA production, nested PCR, sequencing of nested PCR products (search for cloning primers), cloning PCR, plasmid construction, bacteria transformation, plasmid sequencing (search for open reading frames (ORFs)), 293A cell transfection and mAb purification as previously described ( ; ; ) with some modifications. .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Real-time Polymerase Chain Reaction:

    Article Title: Kv4 Channels Underlie the Subthreshold-Operating A-type K+-current in Nociceptive Dorsal Root Ganglion Neurons
    Article Snippet: PCR reactions (20 μL) were performed using 1 μL of cDNA product, 0.5 mM gene specific primers (Table 1 in Supplementary Material), and HotStarTaq Plus Master Mix (Qiagen Inc., Valencia, CA, USA). .. To determine the relative quantities of specific mRNAs by qRT-PCR, we used the Mx2005P QPCR System (Stratagene, La Jolla, CA, USA).

    Multiplex Assay:

    Article Title: Human mining activity across the ages determines the genetic structure of modern brown trout (Salmo trutta L.) populations
    Article Snippet: PCR amplification was carried out using a BIO-RAD MyCycler Thermal Cycler in 10 μ L reaction volumes containing 1 μ L of extracted DNA (c. 30 ng DNA), 3 μ L RNase-free water, 5 μ L of QIAGEN HotStarTaq Plus Master Mix, and 1 μ L of primer mixture, in a total of 8 microsatellite multiplexes ( ). .. PCR conditions were as follows: an initial denaturing step at 95°C for 5 min was followed by 20 cycles of touchdown PCR consisting of 30 s at 94°C, a 30 s annealing step starting at 60°C or 55°C and decreasing by 0.5°C each cycle until a touchdown temperature of 50°C or 45°C (dependent on multiplex; ) was achieved, and an elongation step of 72°C for 30 s, followed by 15 cycles comprising 94°C for 30 s, 50°C or 45°C for 30 s, and 72°C for 30 s. This was followed by a final 10 min extension step at 72°C.

    Article Title: Vaccine-induced plasmablast responses in rhesus macaques: phenotypic characterization and a source for generating antigen-specific monoclonal antibodies
    Article Snippet: Using a multiplex nested PCR, either IgG, IgA or IgM heavy chain and kappa or lambda immunoglobulin (Ig) light chain fragments were amplified from the cDNA using primers recently described ( ). .. The 1st round PCR mix was composed of 2µL of cDNA, 10µL of 2× HotStarTaq Plus Master mix (Qiagen), 0.5µL of outer primers (25µM and 20µM respectively for forward L1 and reverse primers), 0.5µL of 25mM MgCl2 (Promega) and 6.5µL of water, with a final reaction volume of 20µL per well.

    Sample Prep:

    Article Title: The Composition of Midgut Bacteria in Aedes aegypti (Diptera: Culicidae) That Are Naturally Susceptible or Refractory to Dengue Viruses
    Article Snippet: The PCR was performed using the HotStartTaq Plus Master Mix Kit (Qiagen, Hilden, Germany) under the following conditions: 94°C for 3 min, 28 cycles of 94°C for 30 s, 53°C for 40 s, and 72°C for 1 min, after which a final elongation step at 72°C for 5 min was performed. .. A Nextera DNA Sample Preparation Kit was then used (Illumina, CA) to prepare the samples.

    Next-Generation Sequencing:

    Article Title: Feline panleukopenia virus in cerebral neurons of young and adult cats
    Article Snippet: Paragraph title: Next-generation sequencing, confirmation PCRs and sequence analysis ... PCRs were performed using HotStarTaq Plus Master Mix (Qiagen, Hilden, Germany) and conditions were as follows: a denaturation step at 95 °C for 5 min, followed by 45 cycles of 95 °C for 20 s, 55 °C for 45 s, 72 °C for 2 min, and final elongation 5 min at 72 °C.

    Spectrophotometry:

    Article Title: Culex quinquefasciatus larval microbiomes vary with instar and exposure to common wastewater contaminants
    Article Snippet: Upon extraction, nucleic acid concentration was quantified using a Nanodrop ND- 2000c Spectrophotometer (Cole-Palmer, Vernon Hills, IL), to confirm enough genetic material for sequencing. .. The procedure used the forward primer 27Fmod (GRGTTTGATCMTGGCTCAG) and the reverse primer 519Rmodbio (GTNTTACNGCGGCKGCTG) in a single-step 30 cycle PCR using HotStarTaq Plus Master Mix (Qiagen, Valencia, CA).

    Article Title: Study on the association of helicobacter species with viral hepatitis-induced hepatocellular carcinoma
    Article Snippet: DNA concentrations were measured using a “NanoVue” spectrophotometer (GE). .. PCRs were performed using the “HotStarTaq Plus Mastermix kit” from Qiagen in a final volume of 25 ∝l, final concentration of primers was 1 ∝M.

    Produced:

    Article Title: Feline panleukopenia virus in cerebral neurons of young and adult cats
    Article Snippet: A nucleotide sequence identity matrix was produced with Sequence Demarcation Tool version 1.2 [ ]. .. PCRs were performed using HotStarTaq Plus Master Mix (Qiagen, Hilden, Germany) and conditions were as follows: a denaturation step at 95 °C for 5 min, followed by 45 cycles of 95 °C for 20 s, 55 °C for 45 s, 72 °C for 2 min, and final elongation 5 min at 72 °C.

    Activation Assay:

    Article Title: Diversity of Meq gene from clinical Marek’s disease virus infection in Saudi Arabia
    Article Snippet: Detection of the Meq oncoprotein gene The extracted DNAs were screened for presence of MDV using HotStartTaq® Plus Master Mix Kit (QIAGEN, USA). .. Thermo-cycling conditions were enzyme activation and initial denaturation at 95°C for 5 min followed by 35 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 30 s and a final extension step at 72°C for 10 min.

    CTG Assay:

    Article Title: Study on the association of helicobacter species with viral hepatitis-induced hepatocellular carcinoma
    Article Snippet: For pan-Helicobacter PCR assays according to Bohr et al. the following primers (Sigma-Aldrich) were used to amplify the 16S-rRNA genes of Helicobacter ssp. : C97-20 (5'-GGC TAT GAC GGG TAT CCG GC-3') and H3A-20 (5'-GCC GTG CAG CAC CTG TTT TC-3'). .. PCRs were performed using the “HotStarTaq Plus Mastermix kit” from Qiagen in a final volume of 25 ∝l, final concentration of primers was 1 ∝M.

    Staining:

    Article Title: Diversity of Meq gene from clinical Marek’s disease virus infection in Saudi Arabia
    Article Snippet: Detection of the Meq oncoprotein gene The extracted DNAs were screened for presence of MDV using HotStartTaq® Plus Master Mix Kit (QIAGEN, USA). .. The amplified PCR products were electrophoresed in 1.5% agarose gel stained with ethidium bromide and documented using ultraviolet gel documentation system (BIORAD).

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    Qiagen hotstar taq master mix kit
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    Qiagen single step 30 cycle polymerase chain reaction
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