hotfire dna polymerase  (Solis BioDyne)


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    Name:
    HOT FIREPol DNA Polymerase
    Description:
    A chemically modified hot start version of the thermostable Taq DNA polymerase FIREPol This enzyme is activated only by pre incubation at 95°C preventing any unspecific polymerase activity at lower temperatures during reaction set up Increased specificity and sensitivity Reduced primer dimer formation Reaction set up and shipment without ice
    Catalog Number:
    01-02-00500
    Price:
    None
    Category:
    Endpoint PCR
    Applications:
    PCR, qPCR, HOT-start, cloning, sanger sequencing
    Size:
    500 U | 100 µl
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    Structured Review

    Solis BioDyne hotfire dna polymerase
    HOT FIREPol DNA Polymerase
    A chemically modified hot start version of the thermostable Taq DNA polymerase FIREPol This enzyme is activated only by pre incubation at 95°C preventing any unspecific polymerase activity at lower temperatures during reaction set up Increased specificity and sensitivity Reduced primer dimer formation Reaction set up and shipment without ice
    https://www.bioz.com/result/hotfire dna polymerase/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hotfire dna polymerase - by Bioz Stars, 2021-10
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Equine Parvovirus-Hepatitis Screening in Horses and Donkeys with Histopathologic Liver Abnormalities
    Article Snippet: .. Real-time PCR was performed in a volume of 15 μL composed of 1 × PCR buffer B2 (Tris-HCl, (NH4 )2 SO4 and Tween 20; Solis Biodyne, Tartu, Estonia), 3.5 mM MgCl2 , 200 µM of each dNTP (Solis Biodyne), 250 nM of each primer and 200 nM hydrolysis probe (Integrated DNA Technologies, Leuven, Belgium), 1 U HOT FIREPol® DNA polymerase (Solis Biodyne) and 3 μL template DNA. ..

    Polymerase Chain Reaction:

    Article Title: Equine Parvovirus-Hepatitis Screening in Horses and Donkeys with Histopathologic Liver Abnormalities
    Article Snippet: .. Real-time PCR was performed in a volume of 15 μL composed of 1 × PCR buffer B2 (Tris-HCl, (NH4 )2 SO4 and Tween 20; Solis Biodyne, Tartu, Estonia), 3.5 mM MgCl2 , 200 µM of each dNTP (Solis Biodyne), 250 nM of each primer and 200 nM hydrolysis probe (Integrated DNA Technologies, Leuven, Belgium), 1 U HOT FIREPol® DNA polymerase (Solis Biodyne) and 3 μL template DNA. ..

    Article Title: Epigenetic Changes in Equine Embryos after Short-Term Storage at Different Temperatures
    Article Snippet: .. The multiplex PCR was performed in 25 µL reaction volumes including 200 µM of each dNTP, 1 × buffer B2 (Solis BioDyne, Tartu, Estonia), 3 mM MgCl2 , 120 nM of each outer primer, 1.2 units HOT FIREPol DNA polymerase (Solis BioDyne, Tartu, Estonia) and 2 µL bisufite-treated DNA. ..

    Article Title: The Foster method: Rapid and non-invasive detection of clinically relevant American Foulbrood disease levels using eDNA sampling and a dual-target qPCR assay, with its potential for other hive pathogens
    Article Snippet: .. Conventional PCRs used the same 2 μL each DNA dilution in a 20 μL PCR using HOT FIREPol polymerase, ready to load (Solis BioDyne, Tartu, Estonia) and 0.3 μM each primer. ..

    Article Title: Intraspecific Genetic Diversity of Cistus creticus L. and Evolutionary Relationships to Cistus albidus L. (Cistaceae): Meeting of the Generations?
    Article Snippet: .. For a 15 µL PCR reaction, 1 µL of genomic DNA (5-10 ng) was added to a master mix containing 1x PCR buffer B, 2.5 mM MgCl2 , 133 µM dNTP mix, 0.6 units Taq HOT FIREPol® DNA Polymerase (all reagents Solis BioDyne, Tartu, Estonia), and 0.6 µM forward and reverse primers (Life Technologies, Vienna, Austria). ..

    Article Title: Dnmt1 has de novo activity targeted to transposable elements
    Article Snippet: .. PCR was performed in 30-µl reactions with 2 µl of hairpin-bisulfite template and 37 PCR cycles using HotFirePol DNA polymerase (Solis BioDyne) or HotStarTaq DNA polymerase (Qiagen). ..

    Multiplex Assay:

    Article Title: Epigenetic Changes in Equine Embryos after Short-Term Storage at Different Temperatures
    Article Snippet: .. The multiplex PCR was performed in 25 µL reaction volumes including 200 µM of each dNTP, 1 × buffer B2 (Solis BioDyne, Tartu, Estonia), 3 mM MgCl2 , 120 nM of each outer primer, 1.2 units HOT FIREPol DNA polymerase (Solis BioDyne, Tartu, Estonia) and 2 µL bisufite-treated DNA. ..

    Transfection:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    CRISPR:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    Amplification:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    Generated:

    Article Title: The Role of Small Molecules and Their Effect on the Molecular Mechanisms of Early Retinal Organoid Development
    Article Snippet: .. Total RNA was extracted from 5 to 6 organoids per sample using the Qiagen RNeasy Mini kit with a DNase step according to the manufacturer’s instructions. cDNA was generated using Superscript III (Invitrogen, Waltham, MA, USA), and sq-PCR was performed with HOT FIREpol DNA Polymerase (Solis Biodyne, Tartu, Estonia). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. XBP1 splicing was detected by RT-PCR using HOT FIREPol DNA Polymerase (Solis BioDyne) and primers that span the IRE1-mediated splicing event on the XBP1 mRNA ( ). ..

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  • 99
    Solis BioDyne hotfire polymerase
    Hotfire Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotfire polymerase/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hotfire polymerase - by Bioz Stars, 2021-10
    99/100 stars
      Buy from Supplier

    99
    Solis BioDyne dna polymerase
    Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2021-10
    99/100 stars
      Buy from Supplier

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