hot start taq dna polymerase mix (TaKaRa)

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TaKaRa Taq DNA Polymerase Hot Start Version

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The hot start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase thereby preventing DNA synthesis at room temperature During the initial DNA denaturation step reaction temperature 94°C the antibody is denatured releasing the polymerase and allowing DNA synthesis to proceed The use of a hot start PCR enzyme prevents nonspecific amplification due to mispriming and or the formation of primer dimers during PCR assembly

Catalog Number:

r007b

Price:

None

Size:

1 000 Units

Category:

Takara Taq HS Routine PCR master mixes PCR

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Structured Review

TaKaRa hot start taq dna polymerase mix
The hot start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody that binds to Taq polymerase thereby preventing DNA synthesis at room temperature During the initial DNA denaturation step reaction temperature 94°C the antibody is denatured releasing the polymerase and allowing DNA synthesis to proceed The use of a hot start PCR enzyme prevents nonspecific amplification due to mispriming and or the formation of primer dimers during PCR assembly
https://www.bioz.com/result/hot start taq dna polymerase mix/product/TaKaRa
Average 98 stars, based on 2 article reviews
Price from $9.99 to $1999.99

hot start taq dna polymerase mix - by Bioz Stars, 2020-07

98/100 stars

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Polymerase Chain Reaction:

Article Title: From Pig Breeding Environment to Subsequently Produced Pork: Comparative Analysis of Antibiotic Resistance Genes and Bacterial Community Composition
Article Snippet: .. The PCR was performed in a total reaction volume of 50 μL containing 1 μL of 50 ng/μL purified 16S rRNA genes, 5 μL of Taq reaction buffer, 0.2 mM dNTPs, 0.2 μM primers, and 1.25 units of Hot Start Taq DNA polymerase. .. A touchdown PCR was used to amplify the 16S rRNA gene V3-GC region to increase the specificity of the amplification.

Article Title: Replicative mechanisms of CNV formation preferentially occur as intrachromosomal events: evidence from Potocki-Lupski duplication syndrome
Article Snippet: .. PCR amplifications were conducted with TaKaRa Hot start Taq polymerase. .. A 10 μl PCR reaction was performed with 0.1 μl of TaKaRa Hot start Taq polymerase with 1 μl of 10 × PCR buffer, 1.2 μl of dNTPs, 2 pmol of each primer and 10 ng DNA template.

Article Title: Use of 16S rRNA sequencing and quantitative PCR to correlate venous leg ulcer bacterial bioburden dynamics with wound expansion, antibiotic therapy, and healing
Article Snippet: .. PCR reactions contained 16.25 μ L Molecular Biology Grade Water (Fisher BP2819-1), 0.25 μ L TaKaRa Hot Start DNA Polymerase (Clontech TaKaRa R007A) (5 units/ μ L), 2.5 μ L 10× Buffer with MgCl2 (100 mM Tris-HCl (pH 8.3), 500 mM KCl, 15 mM MgCl2 ), 2.0 μ L deoxynucleotide triphosphate (2.5 mM each), 1.0 μ L Primers 515F and 806R working solutions (0.5 μ M final concentration), and 2.0 μ L of total genomic DNA. .. The DNA was denatured for at 98°C for 3 minutes, followed by 35 amplification cycles of 98°C for 10 seconds, 56°C for 30 seconds, 72°C for 60 seconds, and a final extension step of 72°C for 10 minutes.

Article Title: Epigenetic Mutation of RAV6 Affects Leaf Angle and Seed Size in Rice 1 Affects Leaf Angle and Seed Size in Rice 1 [OPEN]
Article Snippet: .. PCR was performed using Hot-Start Taq DNA polymerase (DR007B; Takara Bio). .. Melting curves were read at the end of each amplification by steps of 0.3°C from 65°C to 95°C to ensure that the quantifications were derived from real PCR products and not primer dimers.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Dynamic expression of Epac and Rap1 in mouse oocytes and preimplantation embryos
Article Snippet: .. Epac1 and Rap1a mRNA were detected by RT-PCR with mRNA primer pairs using hot start Taq DNA polymerase (Takara Bio Inc., Shiga, Japan). .. PCR was performed in 96-well plates with 2 l 10 × Taq Buffer, 10 mM deoxy-ribonucleoside triphosphate, 25 mM MgCL2 , primers at a final concentration of 0.2 µm and a cDNA sample derived from 5 ng total RNA in a total volume of 20 µl.

SYBR Green Assay:

Article Title: Genomic Variations in Probiotic Lactobacillus plantarum P-8 in the Human and Rat Gut
Article Snippet: .. The reaction mixture (20 μL) contained 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2 , 200 μM of each dNTP, 500 μg/mL bovine serum albumin (Takara, Dalian, China), a 1:75 000 dilution of SYBR Green I (Takara), 0.4 U Taq DNA polymerase Hot Start version (Takara), 0.2 μM of the specific primers and 2 μL template DNA. .. Isolation of P-8 From Feces and DNA Extraction From Isolated P-8 Colonies After diluting with PBS, the samples were plated and incubated on vancomycin and cycloheximide containing MRS agar under anaerobic conditions for 48 h. To confirm the identity of the P-8 colonies on MRS agar, colony PCR was performed using the strain-specific primers (also used in qPCR).

Purification:

Concentration Assay:

Article Title: Characterization of Phascolarctobacterium succinatutens sp. nov., an Asaccharolytic, Succinate-Utilizing Bacterium Isolated from Human Feces
Article Snippet: .. Each 50-μl reaction mixture contained each deoxynucleoside triphosphate at a concentration of 200 μM, 80 pmol of primer, 3 mM MgCl2 , 1× final concentration of Taq polymerase reaction buffer, 2 U of TaKaRa ExTaq polymerase Hot Start version (Takara Bio, Otsu, Japan), and genomic DNA (50 to 100 ng). ..