hot start hifi mix  (New England Biolabs)


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  • 92

    Structured Review

    New England Biolabs hot start hifi mix
    Hot Start Hifi Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start hifi mix/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot start hifi mix - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    pcr mix
    in-site pcr primers

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    Related Articles

    Microscopy:

    Article Title: Single-cell RNA Sequencing Reveals an Altered Gene Expression Pattern as a Result of CRISPR/cas9-mediated Deletion of Gene 33/Mig6 and Chronic Exposure to Hexavalent Chromium in Human Lung Epithelial Cells
    Article Snippet: Briefly, after trypsinization, single cells were mouth pipetted under a microscope into a 96-well plate with 6.5 μl of lysis buffer in each well. .. PCR mix of 12.5 μl of Q5 2× Hot-Start HiFi mix (New England Bio-labs) and 0.25 μl of 10μM In-Site PCR primers (Exiqon).

    Purification:

    Article Title: Single-cell RNA Sequencing Reveals an Altered Gene Expression Pattern as a Result of CRISPR/cas9-mediated Deletion of Gene 33/Mig6 and Chronic Exposure to Hexavalent Chromium in Human Lung Epithelial Cells
    Article Snippet: PCR mix of 12.5 μl of Q5 2× Hot-Start HiFi mix (New England Bio-labs) and 0.25 μl of 10μM In-Site PCR primers (Exiqon). .. The PCR products were purified using 40 μl of Ampure XP beads (Beckman Coulter).

    Incubation:

    Article Title: Single-cell RNA Sequencing Reveals an Altered Gene Expression Pattern as a Result of CRISPR/cas9-mediated Deletion of Gene 33/Mig6 and Chronic Exposure to Hexavalent Chromium in Human Lung Epithelial Cells
    Article Snippet: PCR mix of 12.5 μl of Q5 2× Hot-Start HiFi mix (New England Bio-labs) and 0.25 μl of 10μM In-Site PCR primers (Exiqon). .. PCR mix of 12.5 μl of Q5 2× Hot-Start HiFi mix (New England Bio-labs) and 0.25 μl of 10μM In-Site PCR primers (Exiqon).

    Polymerase Chain Reaction:

    Article Title: Single-cell RNA Sequencing Reveals an Altered Gene Expression Pattern as a Result of CRISPR/cas9-mediated Deletion of Gene 33/Mig6 and Chronic Exposure to Hexavalent Chromium in Human Lung Epithelial Cells
    Article Snippet: .. PCR mix of 12.5 μl of Q5 2× Hot-Start HiFi mix (New England Bio-labs) and 0.25 μl of 10μM In-Site PCR primers (Exiqon). .. The PCR reaction was as follows: 98°C incubation for 30 seconds; 20 cycles of 98°C for 20 seconds, 67°C for 15 seconds, 72°C for 6 minutes; followed by 5 minutes incubation at 72°C.

    Lysis:

    Article Title: Single-cell RNA Sequencing Reveals an Altered Gene Expression Pattern as a Result of CRISPR/cas9-mediated Deletion of Gene 33/Mig6 and Chronic Exposure to Hexavalent Chromium in Human Lung Epithelial Cells
    Article Snippet: The lysis buffer composed of 4.5 μl of 0.2% Triton X-100 (Sigma-Aldrich), 2 U RNaseOUT, 1.0 μl of 10 mM dNTP (Life Technologies), and 1.0 μl of 10 μM oligo-dT30 VN primer (Exiqon). .. PCR mix of 12.5 μl of Q5 2× Hot-Start HiFi mix (New England Bio-labs) and 0.25 μl of 10μM In-Site PCR primers (Exiqon).

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