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Millipore host bl21
Sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) analysis of recombinant hG31P-P-113 expressed in E. coli <t>BL21.</t>
Host Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/host bl21/product/Millipore
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
host bl21 - by Bioz Stars, 2020-04
93/100 stars

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1) Product Images from "High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli"

Article Title: High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules23040800

Sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) analysis of recombinant hG31P-P-113 expressed in E. coli BL21.
Figure Legend Snippet: Sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) analysis of recombinant hG31P-P-113 expressed in E. coli BL21.

Techniques Used: Polyacrylamide Gel Electrophoresis, Recombinant

2) Product Images from "High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli"

Article Title: High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules23040800

Sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) analysis of recombinant hG31P-P-113 expressed in E. coli BL21.
Figure Legend Snippet: Sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) analysis of recombinant hG31P-P-113 expressed in E. coli BL21.

Techniques Used: Polyacrylamide Gel Electrophoresis, Recombinant

Related Articles

Clone Assay:

Article Title: DMP1 C-Terminal Mutant Mice Recapture the Human ARHR Tooth Phenotype
Article Snippet: Generation of recombinant DMP1 proteins, cell cultures, stains-all staining, and Western blot The full-length DMP1 , C-terminal DMP1 , and full-length delMut DMP1 cDNAs were cloned originally into the pcDNA3 vector. ( ) The empty pcDNA3 vector, pcDNA3-WT DMP1 vector, and pcDNA3-delMut DMP1 vector, were transfected into 293 cell lines for 24 hours, followed by serum-free medium culture for another 24 hours for confirming their secretion in the medium. .. These vectors then were transformed into the bacterial host BL21 for production of recombinant DMP1-Glutathione S-transferase fusion proteins, followed by removal the GST tag using thrombin (Sigma-Aldrich) according to the manufacturer's recommendations.

Construct:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: A second expression plasmid was constructed to produce a His-tagged rib43a protein. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Electrophoresis:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: Pellet proteins were resuspended in 1× SDS buffer (2% SDS [electrophoresis grade, Bio-Rad , Hercules, CA], 192 mM glycine, 25 mM Tris, pH 6.8, 15% glycerol, 0.02% bromphenol blue, 20 mM dithiothreitol, 10% β-mercaptoethanol), incubated at 37°C for 30 min, boiled for 5 min, resolved by SDS-PAGE, and transferred to nitrocellulose. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Incubation:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: Pellet proteins were resuspended in 1× SDS buffer (2% SDS [electrophoresis grade, Bio-Rad , Hercules, CA], 192 mM glycine, 25 mM Tris, pH 6.8, 15% glycerol, 0.02% bromphenol blue, 20 mM dithiothreitol, 10% β-mercaptoethanol), incubated at 37°C for 30 min, boiled for 5 min, resolved by SDS-PAGE, and transferred to nitrocellulose. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Expressing:

Article Title: High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli
Article Snippet: .. The expression vector pET-28a and host BL21 (DE3) were purchased from Novagen (Merck Millipore, Burlington, MA, USA). .. Restriction enzymes and T4 ligase were purchased from Thermo Scientific (Waltham, MA, USA).

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression. .. Colonies were picked immediately after transformation and used to inoculate 50 ml of Luria–Bertani media containing 30 μg/ml kanamycin and 34 μg/ml chloramphenicol.

Article Title: High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli
Article Snippet: .. Materials and Methods The expression vector pET-28a and host BL21 (DE3) were purchased from Novagen (Merck Millipore, Burlington, MA, USA). .. Restriction enzymes and T4 ligase were purchased from Thermo Scientific (Waltham, MA, USA).

Western Blot:

Article Title: Specific complex formation between the type II bare lymphocyte syndrome-associated transactivators CIITA and RFX5
Article Snippet: Recombinant proteins from plasmids pHISCIITTA1–161, pHISCIITA299–1129, and pFUSE were induced in host Bl21(DE3) (Novagen) and purified by nickel-chelate chromatography under denaturing conditions for CIITA-(299–1129) and native conditions for CIITA-(1–161) and GAL4-(1–147) (Qiagen, Chatsworth, CA; protocols 7 and 5, respectively). .. Western blots were made and probed as described ( ) except that 5 μg of recombinant protein was loaded per lane and protein probes were 35 S-radiolabeled by in vitro translation (T n T reticulocyte, Promega).

Article Title: DMP1 C-Terminal Mutant Mice Recapture the Human ARHR Tooth Phenotype
Article Snippet: Paragraph title: Generation of recombinant DMP1 proteins, cell cultures, stains-all staining, and Western blot ... These vectors then were transformed into the bacterial host BL21 for production of recombinant DMP1-Glutathione S-transferase fusion proteins, followed by removal the GST tag using thrombin (Sigma-Aldrich) according to the manufacturer's recommendations.

Transformation Assay:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression. .. Colonies were picked immediately after transformation and used to inoculate 50 ml of Luria–Bertani media containing 30 μg/ml kanamycin and 34 μg/ml chloramphenicol.

Article Title: DMP1 C-Terminal Mutant Mice Recapture the Human ARHR Tooth Phenotype
Article Snippet: .. These vectors then were transformed into the bacterial host BL21 for production of recombinant DMP1-Glutathione S-transferase fusion proteins, followed by removal the GST tag using thrombin (Sigma-Aldrich) according to the manufacturer's recommendations. .. For testing the effects of these recombinant DMP1s on MAPK signaling, an odontoblast cell line( ) was grown to 70% confluency.

Transfection:

Article Title: DMP1 C-Terminal Mutant Mice Recapture the Human ARHR Tooth Phenotype
Article Snippet: Generation of recombinant DMP1 proteins, cell cultures, stains-all staining, and Western blot The full-length DMP1 , C-terminal DMP1 , and full-length delMut DMP1 cDNAs were cloned originally into the pcDNA3 vector. ( ) The empty pcDNA3 vector, pcDNA3-WT DMP1 vector, and pcDNA3-delMut DMP1 vector, were transfected into 293 cell lines for 24 hours, followed by serum-free medium culture for another 24 hours for confirming their secretion in the medium. .. These vectors then were transformed into the bacterial host BL21 for production of recombinant DMP1-Glutathione S-transferase fusion proteins, followed by removal the GST tag using thrombin (Sigma-Aldrich) according to the manufacturer's recommendations.

Chromatography:

Article Title: Specific complex formation between the type II bare lymphocyte syndrome-associated transactivators CIITA and RFX5
Article Snippet: .. Recombinant proteins from plasmids pHISCIITTA1–161, pHISCIITA299–1129, and pFUSE were induced in host Bl21(DE3) (Novagen) and purified by nickel-chelate chromatography under denaturing conditions for CIITA-(299–1129) and native conditions for CIITA-(1–161) and GAL4-(1–147) (Qiagen, Chatsworth, CA; protocols 7 and 5, respectively). .. Western blots were made and probed as described ( ) except that 5 μg of recombinant protein was loaded per lane and protein probes were 35 S-radiolabeled by in vitro translation (T n T reticulocyte, Promega).

Cell Culture:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression. .. Interestingly, colonies picked from the same plates as little as 1 d later or colonies restreaked onto fresh plates failed to produce any His-rib43a protein when cultured and induced.

Sequencing:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: An Nde I site was introduced into the pBrib43a sequence at the initiation codon, using oligonucleotide-directed mutagenesis ( ) to produce the clone pBrib43aNdeI. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Recombinant:

Article Title: Specific complex formation between the type II bare lymphocyte syndrome-associated transactivators CIITA and RFX5
Article Snippet: .. Recombinant proteins from plasmids pHISCIITTA1–161, pHISCIITA299–1129, and pFUSE were induced in host Bl21(DE3) (Novagen) and purified by nickel-chelate chromatography under denaturing conditions for CIITA-(299–1129) and native conditions for CIITA-(1–161) and GAL4-(1–147) (Qiagen, Chatsworth, CA; protocols 7 and 5, respectively). .. Western blots were made and probed as described ( ) except that 5 μg of recombinant protein was loaded per lane and protein probes were 35 S-radiolabeled by in vitro translation (T n T reticulocyte, Promega).

Article Title: DMP1 C-Terminal Mutant Mice Recapture the Human ARHR Tooth Phenotype
Article Snippet: .. These vectors then were transformed into the bacterial host BL21 for production of recombinant DMP1-Glutathione S-transferase fusion proteins, followed by removal the GST tag using thrombin (Sigma-Aldrich) according to the manufacturer's recommendations. .. For testing the effects of these recombinant DMP1s on MAPK signaling, an odontoblast cell line( ) was grown to 70% confluency.

Mutagenesis:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: An Nde I site was introduced into the pBrib43a sequence at the initiation codon, using oligonucleotide-directed mutagenesis ( ) to produce the clone pBrib43aNdeI. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Isolation:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: Paragraph title: Bacterial Expression and Isolation of Fusion Proteins ... This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Purification:

Article Title: Specific complex formation between the type II bare lymphocyte syndrome-associated transactivators CIITA and RFX5
Article Snippet: .. Recombinant proteins from plasmids pHISCIITTA1–161, pHISCIITA299–1129, and pFUSE were induced in host Bl21(DE3) (Novagen) and purified by nickel-chelate chromatography under denaturing conditions for CIITA-(299–1129) and native conditions for CIITA-(1–161) and GAL4-(1–147) (Qiagen, Chatsworth, CA; protocols 7 and 5, respectively). .. Western blots were made and probed as described ( ) except that 5 μg of recombinant protein was loaded per lane and protein probes were 35 S-radiolabeled by in vitro translation (T n T reticulocyte, Promega).

SDS Page:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: Pellet proteins were resuspended in 1× SDS buffer (2% SDS [electrophoresis grade, Bio-Rad , Hercules, CA], 192 mM glycine, 25 mM Tris, pH 6.8, 15% glycerol, 0.02% bromphenol blue, 20 mM dithiothreitol, 10% β-mercaptoethanol), incubated at 37°C for 30 min, boiled for 5 min, resolved by SDS-PAGE, and transferred to nitrocellulose. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Plasmid Preparation:

Article Title: High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli
Article Snippet: .. The expression vector pET-28a and host BL21 (DE3) were purchased from Novagen (Merck Millipore, Burlington, MA, USA). .. Restriction enzymes and T4 ligase were purchased from Thermo Scientific (Waltham, MA, USA).

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: A second expression plasmid was constructed to produce a His-tagged rib43a protein. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Article Title: DMP1 C-Terminal Mutant Mice Recapture the Human ARHR Tooth Phenotype
Article Snippet: Then 5 µL of condition medium was loaded to 4% to 20% gradient polyacrylamide gels, followed by staining with stains-all staining agent, as described previously. ( ) The full-length DMP1 , C-terminal DMP1 , and full-length delMut DMP1 cDNAs were released from the pcDNA3 vector and r-cloned into the PGEX4T-2 vector separately. .. These vectors then were transformed into the bacterial host BL21 for production of recombinant DMP1-Glutathione S-transferase fusion proteins, followed by removal the GST tag using thrombin (Sigma-Aldrich) according to the manufacturer's recommendations.

Article Title: High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli
Article Snippet: .. Materials and Methods The expression vector pET-28a and host BL21 (DE3) were purchased from Novagen (Merck Millipore, Burlington, MA, USA). .. Restriction enzymes and T4 ligase were purchased from Thermo Scientific (Waltham, MA, USA).

Positron Emission Tomography:

Article Title: High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli
Article Snippet: .. The expression vector pET-28a and host BL21 (DE3) were purchased from Novagen (Merck Millipore, Burlington, MA, USA). .. Restriction enzymes and T4 ligase were purchased from Thermo Scientific (Waltham, MA, USA).

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: The Nde I– Xho I fragment from this clone (containing the entire coding region and 3′ untranslated region of rib43a) was isolated from a low-melting-point agarose gel and ligated into pET-28a(+) to produce the His-tagged rib43a-expressing clone pETrib43a. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Article Title: High Level Expression and Purification of the Clinically Active Antimicrobial Peptide P-113 in Escherichia coli
Article Snippet: .. Materials and Methods The expression vector pET-28a and host BL21 (DE3) were purchased from Novagen (Merck Millipore, Burlington, MA, USA). .. Restriction enzymes and T4 ligase were purchased from Thermo Scientific (Waltham, MA, USA).

Agarose Gel Electrophoresis:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: The Nde I– Xho I fragment from this clone (containing the entire coding region and 3′ untranslated region of rib43a) was isolated from a low-melting-point agarose gel and ligated into pET-28a(+) to produce the His-tagged rib43a-expressing clone pETrib43a. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

In Vitro:

Article Title: Specific complex formation between the type II bare lymphocyte syndrome-associated transactivators CIITA and RFX5
Article Snippet: Recombinant proteins from plasmids pHISCIITTA1–161, pHISCIITA299–1129, and pFUSE were induced in host Bl21(DE3) (Novagen) and purified by nickel-chelate chromatography under denaturing conditions for CIITA-(299–1129) and native conditions for CIITA-(1–161) and GAL4-(1–147) (Qiagen, Chatsworth, CA; protocols 7 and 5, respectively). .. Western blots were made and probed as described ( ) except that 5 μg of recombinant protein was loaded per lane and protein probes were 35 S-radiolabeled by in vitro translation (T n T reticulocyte, Promega).

Activation Assay:

Article Title: DMP1 C-Terminal Mutant Mice Recapture the Human ARHR Tooth Phenotype
Article Snippet: These vectors then were transformed into the bacterial host BL21 for production of recombinant DMP1-Glutathione S-transferase fusion proteins, followed by removal the GST tag using thrombin (Sigma-Aldrich) according to the manufacturer's recommendations. .. Cells were treated in triplicate with 250 ng/mL of full-length, C-terminal, or the delMut DMP1 and harvested at different time points (0, 5, 10, 30, and 60 minutes) for MAPK activation.

Far Western Blot:

Article Title: Specific complex formation between the type II bare lymphocyte syndrome-associated transactivators CIITA and RFX5
Article Snippet: Paragraph title: Far-Western Blot Analysis. ... Recombinant proteins from plasmids pHISCIITTA1–161, pHISCIITA299–1129, and pFUSE were induced in host Bl21(DE3) (Novagen) and purified by nickel-chelate chromatography under denaturing conditions for CIITA-(299–1129) and native conditions for CIITA-(1–161) and GAL4-(1–147) (Qiagen, Chatsworth, CA; protocols 7 and 5, respectively).

Staining:

Article Title: The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas
Article Snippet: The strips were stained with Ponceau S and with anti-rib43a antibody; the protein band, which was induced by IPTG and recognized by anti-rib43a, was used to raise rabbit polyclonal antibodies, i.e., anti-rib43aΔN64, as described previously. .. This clone codes for a protein consisting of the 20-amino-acid peptide MGSSHHHHHHSSGLVPRGSH (His tag) followed by the 367 amino acids of the rib43a protein. pETrib43a was transformed into the host BL21(DE3)pLysS (Novagen, Madison, WI) for expression.

Article Title: DMP1 C-Terminal Mutant Mice Recapture the Human ARHR Tooth Phenotype
Article Snippet: Paragraph title: Generation of recombinant DMP1 proteins, cell cultures, stains-all staining, and Western blot ... These vectors then were transformed into the bacterial host BL21 for production of recombinant DMP1-Glutathione S-transferase fusion proteins, followed by removal the GST tag using thrombin (Sigma-Aldrich) according to the manufacturer's recommendations.

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