horseradish peroxidase streptavidin chemiluminesence assay  (Thermo Fisher)


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    Structured Review

    Thermo Fisher horseradish peroxidase streptavidin chemiluminesence assay
    Horseradish Peroxidase Streptavidin Chemiluminesence Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase streptavidin chemiluminesence assay/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase streptavidin chemiluminesence assay - by Bioz Stars, 2020-03
    80/100 stars

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    Related Articles

    Amplification:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce). .. Probe template was synthesized by amplification with PCR primers pANSorfAfor , 5′-GGAAGTAGAAGGCTT-3′, and pANSorfArev , 5′-GGCAATGGCCAGCATCG-3′.

    Agarose Gel Electrophoresis:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Paragraph title: Isolation of Plasmid DNA and Chloroquine Agarose Gel Electrophoresis (CAGE). ... Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Southern Blot:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Southern blot analysis was used to prove that topoisomers visualized by SybrGold staining were from the pANS plasmid. .. Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Synthesized:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce). .. Probe template was synthesized by amplification with PCR primers pANSorfAfor , 5′-GGAAGTAGAAGGCTT-3′, and pANSorfArev , 5′-GGCAATGGCCAGCATCG-3′.

    Isolation:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Paragraph title: Isolation of Plasmid DNA and Chloroquine Agarose Gel Electrophoresis (CAGE). ... Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Buffer Exchange:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Plasmid topoisomers were separated by electrophoresis at 50 volts/14 cm for 24 h in the dark at room temperature with buffer exchange. .. Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Titration:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Chloroquine titration and gyrase inhibition (novobiocin treatment) were used to determine the position of relaxed and supercoiled forms on gel. .. Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Electrophoresis:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Plasmid topoisomers were separated by electrophoresis at 50 volts/14 cm for 24 h in the dark at room temperature with buffer exchange. .. Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Incubation:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Gels were incubated for 1 h in 0.5× TBE and 1× SybrGold stain (Invitrogen). .. Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Polymerase Chain Reaction:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce). .. Probe template was synthesized by amplification with PCR primers pANSorfAfor , 5′-GGAAGTAGAAGGCTT-3′, and pANSorfArev , 5′-GGCAATGGCCAGCATCG-3′.

    Staining:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Southern blot analysis was used to prove that topoisomers visualized by SybrGold staining were from the pANS plasmid. .. Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Inhibition:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Chloroquine titration and gyrase inhibition (novobiocin treatment) were used to determine the position of relaxed and supercoiled forms on gel. .. Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Plasmid Preparation:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Paragraph title: Isolation of Plasmid DNA and Chloroquine Agarose Gel Electrophoresis (CAGE). ... Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce).

    Software:

    Article Title: Oscillations in supercoiling drive circadian gene expression in cyanobacteria
    Article Snippet: Blotting was performed by upward capillary transfer ( ) onto Biodyne B nylon membrane (Pierce) and visualized by horseradish peroxidase-streptavidin chemiluminesence assay (Pierce). .. Gels were analyzed using ImageJ and MATLAB software.