horseradish peroxidase hrp conjugated streptavidin  (Nichirei)

 
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    Structured Review

    Nichirei horseradish peroxidase hrp conjugated streptavidin
    Horseradish Peroxidase Hrp Conjugated Streptavidin, supplied by Nichirei, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated streptavidin/product/Nichirei
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated streptavidin - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Microscopy:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min. .. A BX53 microscope (Olympus, Tokyo, Japan) was used.

    Immunohistochemistry:

    Article Title: Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-α stimulation of synovial cells: an in vitro study
    Article Snippet: Paragraph title: Immunohistochemistry ... Subsequently, the sections were washed twice in PBS and incubated with horseradish peroxidase (HRP)-conjugated streptavidin for 5 min. Peroxidase activity was revealed by 3,3′-diaminobenzidine (Nichirei) and the sections were counterstained with Mayer’s hematoxylin.

    Article Title: Retinoic acid regulates cell-shape and -death of E-FABP (FABP5)-immunoreactive septoclasts in the growth plate cartilage of mice
    Article Snippet: Paragraph title: Immunohistochemistry of E-FABP ... After further rinse, they were reacted for 45 min with horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei).

    Cell Culture:

    Article Title: Oligosaccharides as Receptors for JC Virus
    Article Snippet: Paragraph title: Reagents and cell culture conditions. ... Horseradish peroxidase (HRP)-conjugated streptavidin was obtained from Nichirei (Tokyo, Japan).

    Concentration Assay:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: For antigen retrieval, the sections were dipped into the protease K at 37 °C for 30 min. After washing in PBS, endogenous peroxidase activity was inhibited with normal goat or rabbit serum (Nichirei Co., Tokyo, Japan) for 30 min. Tissue sections were incubated overnight at 4 °C with affinity-purified anti-ADAMTs5 or anti-TSG6 rabbit polyclonal antibodies (Funakoshi, Tokyo, Japan), (abcam204049, Tokyo, Japan) at a concentration of 10 μg/mL each. .. The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min.

    Incubation:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: .. The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min. .. The color reaction was performed with 0.05 Tris-HCl (pH 7.6) containing 3,3′-diaminobendizine tetrahydrochloride (Nichirei Co.) and the sections were counterstained with Meyer’s hematoxylin.

    Article Title: Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-α stimulation of synovial cells: an in vitro study
    Article Snippet: .. Subsequently, the sections were washed twice in PBS and incubated with horseradish peroxidase (HRP)-conjugated streptavidin for 5 min. Peroxidase activity was revealed by 3,3′-diaminobenzidine (Nichirei) and the sections were counterstained with Mayer’s hematoxylin. .. Synovial cell culture Mononuclear cells were isolated from 500 mg synovium by digestion with 20 mL of 0.1% type I collagenase [ ].

    Article Title: Retinoic acid regulates cell-shape and -death of E-FABP (FABP5)-immunoreactive septoclasts in the growth plate cartilage of mice
    Article Snippet: After another rinsing with PBS, they were incubated for 60 min with the secondary antibody, i.e., biotin-conjugated goat anti-rabbit IgG (426012; Nichirei). .. After further rinse, they were reacted for 45 min with horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei).

    Activity Assay:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: For antigen retrieval, the sections were dipped into the protease K at 37 °C for 30 min. After washing in PBS, endogenous peroxidase activity was inhibited with normal goat or rabbit serum (Nichirei Co., Tokyo, Japan) for 30 min. Tissue sections were incubated overnight at 4 °C with affinity-purified anti-ADAMTs5 or anti-TSG6 rabbit polyclonal antibodies (Funakoshi, Tokyo, Japan), (abcam204049, Tokyo, Japan) at a concentration of 10 μg/mL each. .. The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min.

    Article Title: Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-α stimulation of synovial cells: an in vitro study
    Article Snippet: .. Subsequently, the sections were washed twice in PBS and incubated with horseradish peroxidase (HRP)-conjugated streptavidin for 5 min. Peroxidase activity was revealed by 3,3′-diaminobenzidine (Nichirei) and the sections were counterstained with Mayer’s hematoxylin. .. Synovial cell culture Mononuclear cells were isolated from 500 mg synovium by digestion with 20 mL of 0.1% type I collagenase [ ].

    Immunostaining:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: Paragraph title: 4.6. Immunostaining for ADAMTs 5, Stimulated Gene 6 Protein (TSG-6) ... The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min.

    Expressing:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: To examine the expression of ADAMTS5 and TSG-6 in cartilage, the cartilage was stained immunohistochemically with anti-ADAMTS5 antibody and anti-TSG6 antibody. .. The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min.

    Marker:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: Immunostaining for ADAMTs 5, Stimulated Gene 6 Protein (TSG-6) To confirm the anti-inflammatory effect of MSC in the shoulder joint using anti-TSG6 antibody, expression of each factor in the joint cartilage was evaluated immunohistochemically using anti-ADAMTS5 antibody in the cartilage as a marker of shoulder cartilage injury. .. The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min.

    Staining:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: To examine the expression of ADAMTS5 and TSG-6 in cartilage, the cartilage was stained immunohistochemically with anti-ADAMTS5 antibody and anti-TSG6 antibody. .. The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min.

    Affinity Purification:

    Article Title: Intraarticularly-Injected Mesenchymal Stem Cells Stimulate Anti-Inflammatory Molecules and Inhibit Pain Related Protein and Chondrolytic Enzymes in a Monoiodoacetate-Induced Rat Arthritis Model
    Article Snippet: For antigen retrieval, the sections were dipped into the protease K at 37 °C for 30 min. After washing in PBS, endogenous peroxidase activity was inhibited with normal goat or rabbit serum (Nichirei Co., Tokyo, Japan) for 30 min. Tissue sections were incubated overnight at 4 °C with affinity-purified anti-ADAMTs5 or anti-TSG6 rabbit polyclonal antibodies (Funakoshi, Tokyo, Japan), (abcam204049, Tokyo, Japan) at a concentration of 10 μg/mL each. .. The sections were incubated with 10 g/mL biotinylated rabbit anti-mouse immunoglobulin (Nichirei Co.) for 30 min. After washing in PBS, they were incubated with 100 mg/mL horseradish peroxidase (HRP)-conjugated streptavidin (Nichirei Co.) for 30 min.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Transforming growth factor activating kinase 1 regulates extracellular matrix degrading enzymes and pain-related molecule expression following tumor necrosis factor-α stimulation of synovial cells: an in vitro study
    Article Snippet: Subsequently, the sections were incubated overnight at 4 °C with rabbit polyclonal primary antibody against p-TAK1 (Thr184 /187 ) (cat.no. .. Subsequently, the sections were washed twice in PBS and incubated with horseradish peroxidase (HRP)-conjugated streptavidin for 5 min. Peroxidase activity was revealed by 3,3′-diaminobenzidine (Nichirei) and the sections were counterstained with Mayer’s hematoxylin.

    Derivative Assay:

    Article Title: Oligosaccharides as Receptors for JC Virus
    Article Snippet: Horseradish peroxidase (HRP)-conjugated streptavidin was obtained from Nichirei (Tokyo, Japan). .. Sialylneolacto- N -tetraose c (LSTc), GQ1b, and transferrin receptor (human placenta derived) were obtained from Calbiochem (San Diego, Calif.).

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  • 85
    Nichirei hrp streptavidin conjugate
    Hrp Streptavidin Conjugate, supplied by Nichirei, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp streptavidin conjugate/product/Nichirei
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp streptavidin conjugate - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    94
    Nichirei horseradish peroxidase hrp conjugated streptavidin
    Horseradish Peroxidase Hrp Conjugated Streptavidin, supplied by Nichirei, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated streptavidin/product/Nichirei
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated streptavidin - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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