horseradish peroxidase conjugated secondary antibodies  (The Jackson Laboratory)

 
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    Structured Review

    The Jackson Laboratory horseradish peroxidase conjugated secondary antibodies
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibodies/product/The Jackson Laboratory
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    92/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Susceptibility to Vibrio cholerae Infection in a Cohort of Household Contacts of Patients with Cholera in Bangladesh
    Article Snippet: .. Plates were incubated with diluted patient sera (1∶50 for LPS ELISA, 1∶100 for TCP, and 1∶200 for CTB), washed, and horseradish peroxidase-conjugated secondary antibodies to either human IgG or IgA were applied (Jackson Laboratories, Bar Harbor, Maine). .. Plates were developed using 0.1% ortho-phenylene diamine (Sigma, St. Louis, Missouri) in 0.1 M sodium citrate buffer with 0.1% hydrogen peroxide, and optical densities (OD) were read kinetically at 450 nm for 5 minutes at 19-s intervals.

    CtB Assay:

    Article Title: Susceptibility to Vibrio cholerae Infection in a Cohort of Household Contacts of Patients with Cholera in Bangladesh
    Article Snippet: .. Plates were incubated with diluted patient sera (1∶50 for LPS ELISA, 1∶100 for TCP, and 1∶200 for CTB), washed, and horseradish peroxidase-conjugated secondary antibodies to either human IgG or IgA were applied (Jackson Laboratories, Bar Harbor, Maine). .. Plates were developed using 0.1% ortho-phenylene diamine (Sigma, St. Louis, Missouri) in 0.1 M sodium citrate buffer with 0.1% hydrogen peroxide, and optical densities (OD) were read kinetically at 450 nm for 5 minutes at 19-s intervals.

    Incubation:

    Article Title: Essential Roles of mTOR/Akt Pathway in Aurora-A Cell Transformation
    Article Snippet: .. Membranes were blocked in 1% nonfat dried milk in phosphate-buffered saline/0.05% Tween and incubated with primary antibodies and horseradish peroxidase-conjugated secondary antibodies (Jackson Laboratories, Westgrove, PA) followed by ECL detection. .. For immunoprecipitation, 1 mg of total cell lysate was incubated with the specific antibody over night at 4ºC followed by either protein A- or protein-G Sepharose beads (Sigma, St. Louis, MO).

    Article Title: Susceptibility to Vibrio cholerae Infection in a Cohort of Household Contacts of Patients with Cholera in Bangladesh
    Article Snippet: .. Plates were incubated with diluted patient sera (1∶50 for LPS ELISA, 1∶100 for TCP, and 1∶200 for CTB), washed, and horseradish peroxidase-conjugated secondary antibodies to either human IgG or IgA were applied (Jackson Laboratories, Bar Harbor, Maine). .. Plates were developed using 0.1% ortho-phenylene diamine (Sigma, St. Louis, Missouri) in 0.1 M sodium citrate buffer with 0.1% hydrogen peroxide, and optical densities (OD) were read kinetically at 450 nm for 5 minutes at 19-s intervals.

    Article Title: OPA1-anchored PKA phosphorylates perilipin 1 on S522 and S497 in adipocytes differentiated from human adipose stem cells
    Article Snippet: .. After incubation with appropriate horseradish peroxidase–conjugated secondary antibody (1:10,000; Jackson laboratories), blots were washed five times and then developed using Super Signal West Pico/Dura substrate (Pierce). .. Immunolocalization studies Immunofluorescence stainings were performed on undifferentiated and adipocyte-differentiated ASC at 1, 2, and 3 wk under adipogenic differentiation.

    Western Blot:

    Article Title: Connexin43 mutations linked to skin disease have augmented hemichannel activity
    Article Snippet: .. Western blots were first blocked with 5% milk 0.1% Tween20 in TBS, then probed with polyclonal antibodies against Cx43 (Life Technologies, Carlsbad CA), followed by horseradish peroxidase conjugated secondary antibodies (Jackson Laboratories and GE Healthcare). .. A monoclonal β-tubulin antibody (Abcam, Cambridge, MA) was used as a loading control.

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    The Jackson Laboratory goat anti mouse hrp conjugated secondary antibodies
    An N-glycosylation analysis of the fE1E2 and tE1E2 complex expressed in L. tarentolae . ( A ) The recombinant complexes were treated with endoglycosidase PNGaseF. After overnight incubation at 37 °C in native conditions, western blot under reducing conditions with <t>anti-E2</t> Ab was performed. The E1E2 complex expressed in HEK 293 cells was used as the control (hE1E2). ( B ) Dose-dependent binding of the E1E2 complexes to GNA lectin. Decreasing concentrations of cell lysates (5-fold dilutions - from 1:5 to 1:3125) were captured in ELISA plates coated with GNA lectin. The bound antigens were visualized with anti-E2 Abs, anti-mouse IgG <t>HRP</t> conjugate, and the TMB substrate. The error bars represent the standard deviations of 2 replicate values.
    Goat Anti Mouse Hrp Conjugated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse hrp conjugated secondary antibodies/product/The Jackson Laboratory
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    86
    The Jackson Laboratory horseradish peroxidase conjugated polyclonal secondary antibody
    Reactivation of virus specific CD4 + T cells was enhanced upon antigen-presenting cell (APC) exposure to vaccinia virus (VV) encoding murine CD74 (mCD74-VV). (a) Splenic APC were cultured in vitro with VV [multiplicity of infection (MOI) 0·1], mCD74-VV (MOI 0·1), or without virus (Cont.) for 14 hr, followed by incubation with VV-primed CD4 + T cells. T-cell proliferation in response to virus-treated APC was monitored. T-cell responses to VV-infected APC were set equal to 1 for comparison to uninfected (Cont.) or mCD74-VV-infected splenic APC. (b) Flow cytometric analysis of splenic APC revealed a similar pattern and level of virus-infected B cells, dendritic cells (DC), and macrophages after in vitro infection with VV or mCD74-VV (MOI 1) at 24 hr (VV was not detectable at an MOI of 0·1). Virus-infected cells were detected using a <t>polyclonal</t> anti-VV antibody. Studies are representative of three independent experiments, mean ± SD. One-way analysis of variance, Dunnett's multiple comparisons test.
    Horseradish Peroxidase Conjugated Polyclonal Secondary Antibody, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated polyclonal secondary antibody/product/The Jackson Laboratory
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated polyclonal secondary antibody - by Bioz Stars, 2020-07
    86/100 stars
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    93
    The Jackson Laboratory hrp conjugated secondary antibodies
    Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with <t>streptavidin-HRP</t> to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value
    Hrp Conjugated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated secondary antibodies/product/The Jackson Laboratory
    Average 93 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
    93/100 stars
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    88
    The Jackson Laboratory horseradish peroxidase conjugated antihuman igg
    Fold increases between days 2 and 7 in serum <t>IgG</t> (black bars) and <t>IgA</t> (shaded bars) to LPS in children compared to older patients with cholera. Error bars represent the 95% CI of the means. * P = 0.03 and **P = 0.0008 for the differences in the magnitude
    Horseradish Peroxidase Conjugated Antihuman Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated antihuman igg/product/The Jackson Laboratory
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated antihuman igg - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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    An N-glycosylation analysis of the fE1E2 and tE1E2 complex expressed in L. tarentolae . ( A ) The recombinant complexes were treated with endoglycosidase PNGaseF. After overnight incubation at 37 °C in native conditions, western blot under reducing conditions with anti-E2 Ab was performed. The E1E2 complex expressed in HEK 293 cells was used as the control (hE1E2). ( B ) Dose-dependent binding of the E1E2 complexes to GNA lectin. Decreasing concentrations of cell lysates (5-fold dilutions - from 1:5 to 1:3125) were captured in ELISA plates coated with GNA lectin. The bound antigens were visualized with anti-E2 Abs, anti-mouse IgG HRP conjugate, and the TMB substrate. The error bars represent the standard deviations of 2 replicate values.

    Journal: Scientific Reports

    Article Title: Immunogenicity and functional characterization of Leishmania-derived hepatitis C virus envelope glycoprotein complex

    doi: 10.1038/srep30627

    Figure Lengend Snippet: An N-glycosylation analysis of the fE1E2 and tE1E2 complex expressed in L. tarentolae . ( A ) The recombinant complexes were treated with endoglycosidase PNGaseF. After overnight incubation at 37 °C in native conditions, western blot under reducing conditions with anti-E2 Ab was performed. The E1E2 complex expressed in HEK 293 cells was used as the control (hE1E2). ( B ) Dose-dependent binding of the E1E2 complexes to GNA lectin. Decreasing concentrations of cell lysates (5-fold dilutions - from 1:5 to 1:3125) were captured in ELISA plates coated with GNA lectin. The bound antigens were visualized with anti-E2 Abs, anti-mouse IgG HRP conjugate, and the TMB substrate. The error bars represent the standard deviations of 2 replicate values.

    Article Snippet: The binding to the lectin was determined using anti-E2 AP33 (1:2000) or ALP98 (1:1000) antibody followed by incubation with goat anti-mouse HRP-conjugated secondary antibodies diluted 1:2000 (The Jackson Laboratory) and the tetramethylbenzidine (TMB) substrate.

    Techniques: Recombinant, Incubation, Western Blot, Binding Assay, Enzyme-linked Immunosorbent Assay

    Reactivation of virus specific CD4 + T cells was enhanced upon antigen-presenting cell (APC) exposure to vaccinia virus (VV) encoding murine CD74 (mCD74-VV). (a) Splenic APC were cultured in vitro with VV [multiplicity of infection (MOI) 0·1], mCD74-VV (MOI 0·1), or without virus (Cont.) for 14 hr, followed by incubation with VV-primed CD4 + T cells. T-cell proliferation in response to virus-treated APC was monitored. T-cell responses to VV-infected APC were set equal to 1 for comparison to uninfected (Cont.) or mCD74-VV-infected splenic APC. (b) Flow cytometric analysis of splenic APC revealed a similar pattern and level of virus-infected B cells, dendritic cells (DC), and macrophages after in vitro infection with VV or mCD74-VV (MOI 1) at 24 hr (VV was not detectable at an MOI of 0·1). Virus-infected cells were detected using a polyclonal anti-VV antibody. Studies are representative of three independent experiments, mean ± SD. One-way analysis of variance, Dunnett's multiple comparisons test.

    Journal: Immunology

    Article Title: Virus-encoded ectopic CD74 enhances poxvirus vaccine efficacy

    doi: 10.1111/imm.12210

    Figure Lengend Snippet: Reactivation of virus specific CD4 + T cells was enhanced upon antigen-presenting cell (APC) exposure to vaccinia virus (VV) encoding murine CD74 (mCD74-VV). (a) Splenic APC were cultured in vitro with VV [multiplicity of infection (MOI) 0·1], mCD74-VV (MOI 0·1), or without virus (Cont.) for 14 hr, followed by incubation with VV-primed CD4 + T cells. T-cell proliferation in response to virus-treated APC was monitored. T-cell responses to VV-infected APC were set equal to 1 for comparison to uninfected (Cont.) or mCD74-VV-infected splenic APC. (b) Flow cytometric analysis of splenic APC revealed a similar pattern and level of virus-infected B cells, dendritic cells (DC), and macrophages after in vitro infection with VV or mCD74-VV (MOI 1) at 24 hr (VV was not detectable at an MOI of 0·1). Virus-infected cells were detected using a polyclonal anti-VV antibody. Studies are representative of three independent experiments, mean ± SD. One-way analysis of variance, Dunnett's multiple comparisons test.

    Article Snippet: VV antigens were detected using a rabbit antiserum to viral H3L or anti-VV polyclonal antibody (Cortex Biochem, San Leandro, CA) followed by horseradish peroxidase-conjugated polyclonal secondary antibody (Jackson Laboratory).

    Techniques: Cell Culture, In Vitro, Infection, Incubation, Flow Cytometry

    Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with streptavidin-HRP to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value

    Journal: PLoS ONE

    Article Title: Degradation of Internalized ?v?5 Integrin Is Controlled by uPAR Bound uPA: Effect on ?1 Integrin Activity and ?-SMA Stress Fiber Assembly

    doi: 10.1371/journal.pone.0033915

    Figure Lengend Snippet: Integrin β5 protein expression is increased on TGFβ-induced Mfs. HCFs were treated with TGFβ1 or FGF-2 plus heparin for 72 hours and analyzed for β5 and β3 cell-surface expression. (A) Flow cytometry (B) Cell-surface biotinylation; lysates were immunoprecipitated (IP) with antibody against integrin β5, separated by SDS-PAGE and probed with streptavidin-HRP to detect biotinylated proteins. Bands were identified by molecular weight. GAPDH indicates equal protein input for IP. Quantification of the β5 band relative to GAPDH input control is shown. (C) Real-time PCR for β5, β3, and uPAR at Day 1 and Day 7 after TGFβ1 treatment. *p value

    Article Snippet: HRP-conjugated anti-Streptavidin antibody and all HRP-conjugated secondary antibodies were from Jackson Laboratories.

    Techniques: Expressing, Flow Cytometry, Cytometry, Immunoprecipitation, SDS Page, Molecular Weight, Real-time Polymerase Chain Reaction

    Fold increases between days 2 and 7 in serum IgG (black bars) and IgA (shaded bars) to LPS in children compared to older patients with cholera. Error bars represent the 95% CI of the means. * P = 0.03 and **P = 0.0008 for the differences in the magnitude

    Journal: The Pediatric infectious disease journal

    Article Title: A Comparison of Clinical and Immunologic Features in Children and Older Patients Hospitalized With Severe Cholera in Bangladesh

    doi: 10.1097/INF.0b013e3181783adf

    Figure Lengend Snippet: Fold increases between days 2 and 7 in serum IgG (black bars) and IgA (shaded bars) to LPS in children compared to older patients with cholera. Error bars represent the 95% CI of the means. * P = 0.03 and **P = 0.0008 for the differences in the magnitude

    Article Snippet: Plates were incubated with diluted patient sera, washed, and secondary, horseradish peroxidase-conjugated antihuman IgG or antihuman IgA antibodies were applied (Jackson Laboratories, Bar Harbor, ME).

    Techniques:

    Fold increases between days 2 and 7 in serum IgG (black bars) and IgA (shaded bars) to CTB in children compared to older patients with cholera. Error bars represent the 95% CI of the means. * P = 0.002 for the difference in the magnitude of rises in IgA

    Journal: The Pediatric infectious disease journal

    Article Title: A Comparison of Clinical and Immunologic Features in Children and Older Patients Hospitalized With Severe Cholera in Bangladesh

    doi: 10.1097/INF.0b013e3181783adf

    Figure Lengend Snippet: Fold increases between days 2 and 7 in serum IgG (black bars) and IgA (shaded bars) to CTB in children compared to older patients with cholera. Error bars represent the 95% CI of the means. * P = 0.002 for the difference in the magnitude of rises in IgA

    Article Snippet: Plates were incubated with diluted patient sera, washed, and secondary, horseradish peroxidase-conjugated antihuman IgG or antihuman IgA antibodies were applied (Jackson Laboratories, Bar Harbor, ME).

    Techniques: CtB Assay

    Fold increases between days 2 and 7 in serum IgG (black bars) and IgA antibodies (shaded bars) to TcpA children compared to older patients with cholera. Error bars represent the 95% CI of the means. * P = 0.04 and ** P = 0.03 for the differences in the

    Journal: The Pediatric infectious disease journal

    Article Title: A Comparison of Clinical and Immunologic Features in Children and Older Patients Hospitalized With Severe Cholera in Bangladesh

    doi: 10.1097/INF.0b013e3181783adf

    Figure Lengend Snippet: Fold increases between days 2 and 7 in serum IgG (black bars) and IgA antibodies (shaded bars) to TcpA children compared to older patients with cholera. Error bars represent the 95% CI of the means. * P = 0.04 and ** P = 0.03 for the differences in the

    Article Snippet: Plates were incubated with diluted patient sera, washed, and secondary, horseradish peroxidase-conjugated antihuman IgG or antihuman IgA antibodies were applied (Jackson Laboratories, Bar Harbor, ME).

    Techniques: