horseradish peroxidase conjugated secondary antibodies  (GeneTex)

 
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    Structured Review

    GeneTex horseradish peroxidase conjugated secondary antibodies
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibodies/product/GeneTex
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    90/100 stars

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    Western Blot:

    Article Title: Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure
    Article Snippet: .. For western blotting, the membranes were blocked with 2% ECL Prime Blocking Reagent (GE Healthcare) in TBS-T buffer (25 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.4) at room temperature for 1 h, incubated overnight at 4°C with primary antibodies, and then incubated with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h. Primary antibodies against the following proteins were used: rat β-actin (ACTB, 1:10,000; #109639, GeneTex, San Antonio, TX), glycoprotein-N -acetylgalactosamine 3-β-galactosyltransferase (C1GALT1,1:1,000; #46062, GeneTex), C1GALT1 molecular chaperone (C1GALT1C1, 1:1,000; #115877, GeneTex), α-l -fucosidase (FUCA1, 1:1,000; #AV54293, Sigma-Aldrich), N -acetylgalactosaminyltransferase 1 (GALNT1, 1:300; #sc-68492, Santa Cruz Biotechnology), GALNT2 (1:2,000; #104070, GeneTex), GALNT7 (1:10,000; #106068, GeneTex), glycerardehyde-3-phosphate dehydrogenase (GAPDH, 1:1,000; #2118, Cell Signaling Technology, Beverly, MA), β-galactosidase (GLB1, 1:3,000; #104360, GeneTex), O -GlcNAc hydrolase (OGA, 1:500; #14711-1-AP, Proteintech, Chicago, IL), O -GlcNAc transferase (OGT, 1:3,000; #109939, GeneTex), and CSRP3 (1: 2000; #88505, GeneTex). .. Horseradish peroxidase-conjugated secondary antibodies were goat anti-rabbit IgG (H+L) (1:2,000, #7074; Cell Signaling Technology) and rabbit anti-goat IgG (H+L) (1:2,000, #305-035-003; Jackson ImmunoResearch, West Grove, PA).

    Incubation:

    Article Title: Loss of Response Gene to Complement 32 (RGC-32) in Diabetic Mouse Retina Is Involved in Retinopathy Development
    Article Snippet: .. Membranes were blocked with blocking buffer (Sigma Aldrich, St. Louis, MI, USA) and incubated with primary antibodies (Abs) overnight at 4 °C followed by incubation with horseradish peroxidase conjugated secondary antibody (GeneTex, TX, USA) at room temperature for 1 h. Primary Abs used in the study were RGC-32 (dilution 1:200, Santa Cruz Biotechnology, TX, USA), BCL2-associated X (Bax) (dilution 1:100, Thermo Scientific, Cheshire, UK), B-cell lymphoma 2 (Bcl-2) (dilution 1:1000, Cell Signaling Technology, Danvers, MA, USA), and cleaved caspase-3 (dilution 1:500, Cell Signaling Technology, MA, USA). .. Anti-β-actin (dilution 1:5000, Novus Biological, Centennial, CO, USA) was used as an internal control.

    Article Title: Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure
    Article Snippet: .. For western blotting, the membranes were blocked with 2% ECL Prime Blocking Reagent (GE Healthcare) in TBS-T buffer (25 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.4) at room temperature for 1 h, incubated overnight at 4°C with primary antibodies, and then incubated with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h. Primary antibodies against the following proteins were used: rat β-actin (ACTB, 1:10,000; #109639, GeneTex, San Antonio, TX), glycoprotein-N -acetylgalactosamine 3-β-galactosyltransferase (C1GALT1,1:1,000; #46062, GeneTex), C1GALT1 molecular chaperone (C1GALT1C1, 1:1,000; #115877, GeneTex), α-l -fucosidase (FUCA1, 1:1,000; #AV54293, Sigma-Aldrich), N -acetylgalactosaminyltransferase 1 (GALNT1, 1:300; #sc-68492, Santa Cruz Biotechnology), GALNT2 (1:2,000; #104070, GeneTex), GALNT7 (1:10,000; #106068, GeneTex), glycerardehyde-3-phosphate dehydrogenase (GAPDH, 1:1,000; #2118, Cell Signaling Technology, Beverly, MA), β-galactosidase (GLB1, 1:3,000; #104360, GeneTex), O -GlcNAc hydrolase (OGA, 1:500; #14711-1-AP, Proteintech, Chicago, IL), O -GlcNAc transferase (OGT, 1:3,000; #109939, GeneTex), and CSRP3 (1: 2000; #88505, GeneTex). .. Horseradish peroxidase-conjugated secondary antibodies were goat anti-rabbit IgG (H+L) (1:2,000, #7074; Cell Signaling Technology) and rabbit anti-goat IgG (H+L) (1:2,000, #305-035-003; Jackson ImmunoResearch, West Grove, PA).

    Article Title: MLN4924, a Protein Neddylation Inhibitor, Suppresses the Growth of Human Chondrosarcoma through Inhibiting Cell Proliferation and Inducing Endoplasmic Reticulum Stress-Related Apoptosis
    Article Snippet: .. The resulting membranes were then washed twice using TBST for 10 min and incubated with specific horseradish peroxidase-conjugated secondary antibodies (Genetex, Irvine, CA, USA) for 1 h at room temperature. ..

    Article Title: The Deubiquitinating Enzyme Inhibitor PR-619 Enhances the Cytotoxicity of Cisplatin via the Suppression of Anti-Apoptotic Bcl-2 Protein: In Vitro and In Vivo Study
    Article Snippet: .. After being washed twice with TBST (tris buffered saline containing 0.05% Tween 20), the membrane was incubated with horseradish-peroxidase-conjugated secondary antibodies (GeneTex) at room temperature for 2 h. The antibody-labeled membrane was washed twice with TBST. .. Then, enhanced chemiluminescence (ECL) substrates (Merck Millipore and Biotools) were used for detection in an ImageQuant LAS 4000 (GE Healthcare, Chicago, IL, USA) system [ ].

    Article Title: Trifluoperazine, an Antipsychotic Drug, Effectively Reduces Drug Resistance in Cisplatin-Resistant Urothelial Carcinoma Cells via Suppressing Bcl-xL: An In Vitro and In Vivo Study
    Article Snippet: .. After being washed twice with TBST (TBS containing 0.05% Tween 20), the membranes were incubated with horseradish-peroxidase-conjugated secondary antibodies (GeneTex) at recommended dilution ratios in TBST at room temperature for 2 h. The antibody-labeled membranes were again washed twice with TBST and visualized by enhanced chemiluminescence (ECL) substrates (Merck Millipore and Biotools) under an ImageQuant LAS 4000 (GE Healthcare, Chicago, IL, USA) system. ..

    Blocking Assay:

    Article Title: Loss of Response Gene to Complement 32 (RGC-32) in Diabetic Mouse Retina Is Involved in Retinopathy Development
    Article Snippet: .. Membranes were blocked with blocking buffer (Sigma Aldrich, St. Louis, MI, USA) and incubated with primary antibodies (Abs) overnight at 4 °C followed by incubation with horseradish peroxidase conjugated secondary antibody (GeneTex, TX, USA) at room temperature for 1 h. Primary Abs used in the study were RGC-32 (dilution 1:200, Santa Cruz Biotechnology, TX, USA), BCL2-associated X (Bax) (dilution 1:100, Thermo Scientific, Cheshire, UK), B-cell lymphoma 2 (Bcl-2) (dilution 1:1000, Cell Signaling Technology, Danvers, MA, USA), and cleaved caspase-3 (dilution 1:500, Cell Signaling Technology, MA, USA). .. Anti-β-actin (dilution 1:5000, Novus Biological, Centennial, CO, USA) was used as an internal control.

    Article Title: Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure
    Article Snippet: .. For western blotting, the membranes were blocked with 2% ECL Prime Blocking Reagent (GE Healthcare) in TBS-T buffer (25 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.4) at room temperature for 1 h, incubated overnight at 4°C with primary antibodies, and then incubated with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h. Primary antibodies against the following proteins were used: rat β-actin (ACTB, 1:10,000; #109639, GeneTex, San Antonio, TX), glycoprotein-N -acetylgalactosamine 3-β-galactosyltransferase (C1GALT1,1:1,000; #46062, GeneTex), C1GALT1 molecular chaperone (C1GALT1C1, 1:1,000; #115877, GeneTex), α-l -fucosidase (FUCA1, 1:1,000; #AV54293, Sigma-Aldrich), N -acetylgalactosaminyltransferase 1 (GALNT1, 1:300; #sc-68492, Santa Cruz Biotechnology), GALNT2 (1:2,000; #104070, GeneTex), GALNT7 (1:10,000; #106068, GeneTex), glycerardehyde-3-phosphate dehydrogenase (GAPDH, 1:1,000; #2118, Cell Signaling Technology, Beverly, MA), β-galactosidase (GLB1, 1:3,000; #104360, GeneTex), O -GlcNAc hydrolase (OGA, 1:500; #14711-1-AP, Proteintech, Chicago, IL), O -GlcNAc transferase (OGT, 1:3,000; #109939, GeneTex), and CSRP3 (1: 2000; #88505, GeneTex). .. Horseradish peroxidase-conjugated secondary antibodies were goat anti-rabbit IgG (H+L) (1:2,000, #7074; Cell Signaling Technology) and rabbit anti-goat IgG (H+L) (1:2,000, #305-035-003; Jackson ImmunoResearch, West Grove, PA).

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