horseradish peroxidase conjugated secondary antibodies  (Boehringer Mannheim)

 
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    Structured Review

    Boehringer Mannheim horseradish peroxidase conjugated secondary antibodies
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibodies/product/Boehringer Mannheim
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    92/100 stars

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    Incubation:

    Article Title: NFI-Ski Interactions Mediate Transforming Growth Factor ? Modulation of Human Papillomavirus Type 16 Early Gene Expression
    Article Snippet: .. The blot was then incubated with an appropriate horseradish peroxidase-conjugated secondary antibody (Boehringer Mannheim; diluted 1:5,000 in blocking solution) for 1 h at room temperature. .. The Super Signal West Pico chemiluminescence detection kit (Pierce) was used for detection.

    Article Title: The Arabidopsis KNOLLE Protein Is a Cytokinesis-specific Syntaxin
    Article Snippet: .. The filter was blocked with 5% (wt/vol) nonfat dry milk in Tris-buffered saline with 0.2% Tween 20 (TBST: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.2% Tween 20), probed with primary antibody, washed with TBST plus 400 mM NaCl and 0.8% Tween 20, incubated with horseradish peroxidase–conjugated secondary antibody diluted 1:2,000 in blocking buffer, and washed again before detection with the BM chemiluminescence system ( Boehringer Mannheim Corp. , Indianapolis, IN). ..

    Blocking Assay:

    Article Title: NFI-Ski Interactions Mediate Transforming Growth Factor ? Modulation of Human Papillomavirus Type 16 Early Gene Expression
    Article Snippet: .. The blot was then incubated with an appropriate horseradish peroxidase-conjugated secondary antibody (Boehringer Mannheim; diluted 1:5,000 in blocking solution) for 1 h at room temperature. .. The Super Signal West Pico chemiluminescence detection kit (Pierce) was used for detection.

    Article Title: The Arabidopsis KNOLLE Protein Is a Cytokinesis-specific Syntaxin
    Article Snippet: .. The filter was blocked with 5% (wt/vol) nonfat dry milk in Tris-buffered saline with 0.2% Tween 20 (TBST: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.2% Tween 20), probed with primary antibody, washed with TBST plus 400 mM NaCl and 0.8% Tween 20, incubated with horseradish peroxidase–conjugated secondary antibody diluted 1:2,000 in blocking buffer, and washed again before detection with the BM chemiluminescence system ( Boehringer Mannheim Corp. , Indianapolis, IN). ..

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