hoechst 33288  (Dapco Industries)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Dapco Industries hoechst 33288
    Hoechst 33288, supplied by Dapco Industries, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288/product/Dapco Industries
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 - by Bioz Stars, 2024-10
    86/100 stars

    Images

    hoechst 33288  (Dapco Industries)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Dapco Industries hoechst 33288
    PilA2 assembly into extracellular filaments depends on pilE , the operon lpp1976–lpp1978 , and pilZ . (A) Visualization of PilA2-FLAG filaments (green) by immunofluorescence microscopy using fluorescein-conjugated anti-FLAG antibody. Bacteria were visualized by labeling DNA with <t>Hoechst</t> <t>33288</t> (magenta). (B) Western blot detection of extracellular PilA2. Bacteria were vortexed to release pili which were precipitated from supernatants. PilA2-FLAG was detected in supernatant and whole-cell lysates (pellet) using anti-FLAG antibodies. (C) DNA uptake assay of the reconstructed mutants defective for natural transformation. The transformation-deficient mutants were tested for the ability to internalize pGEM-HYG1, a nonreplicative plasmid. Following incubation with the DNA and subsequent DNase I treatment, the internalized DNA was detected in cells by PCR for pGEM-HYG. As a control, chromosomal DNA was also detected by PCR. This multiplex PCR was analyzed by agarose gel electrophoresis and labeling of DNA with ethidium bromide. Images are representative of experiments performed three times independently.
    Hoechst 33288, supplied by Dapco Industries, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288/product/Dapco Industries
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1) Product Images from "Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation"

    Article Title: Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.00548-20

    PilA2 assembly into extracellular filaments depends on pilE , the operon lpp1976–lpp1978 , and pilZ . (A) Visualization of PilA2-FLAG filaments (green) by immunofluorescence microscopy using fluorescein-conjugated anti-FLAG antibody. Bacteria were visualized by labeling DNA with Hoechst 33288 (magenta). (B) Western blot detection of extracellular PilA2. Bacteria were vortexed to release pili which were precipitated from supernatants. PilA2-FLAG was detected in supernatant and whole-cell lysates (pellet) using anti-FLAG antibodies. (C) DNA uptake assay of the reconstructed mutants defective for natural transformation. The transformation-deficient mutants were tested for the ability to internalize pGEM-HYG1, a nonreplicative plasmid. Following incubation with the DNA and subsequent DNase I treatment, the internalized DNA was detected in cells by PCR for pGEM-HYG. As a control, chromosomal DNA was also detected by PCR. This multiplex PCR was analyzed by agarose gel electrophoresis and labeling of DNA with ethidium bromide. Images are representative of experiments performed three times independently.
    Figure Legend Snippet: PilA2 assembly into extracellular filaments depends on pilE , the operon lpp1976–lpp1978 , and pilZ . (A) Visualization of PilA2-FLAG filaments (green) by immunofluorescence microscopy using fluorescein-conjugated anti-FLAG antibody. Bacteria were visualized by labeling DNA with Hoechst 33288 (magenta). (B) Western blot detection of extracellular PilA2. Bacteria were vortexed to release pili which were precipitated from supernatants. PilA2-FLAG was detected in supernatant and whole-cell lysates (pellet) using anti-FLAG antibodies. (C) DNA uptake assay of the reconstructed mutants defective for natural transformation. The transformation-deficient mutants were tested for the ability to internalize pGEM-HYG1, a nonreplicative plasmid. Following incubation with the DNA and subsequent DNase I treatment, the internalized DNA was detected in cells by PCR for pGEM-HYG. As a control, chromosomal DNA was also detected by PCR. This multiplex PCR was analyzed by agarose gel electrophoresis and labeling of DNA with ethidium bromide. Images are representative of experiments performed three times independently.

    Techniques Used: Immunofluorescence, Microscopy, Labeling, Western Blot, Transformation Assay, Plasmid Preparation, Incubation, Multiplex Assay, Agarose Gel Electrophoresis

    hoechst 33288  (Dapco Industries)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Dapco Industries hoechst 33288
    PilA_2 assembly into extracellular filaments depends on pilE , the operon lpp1976-8 and pilZ . A) Visualization of PilA_2-FLAG filaments (green) by immunofluorescence microscopy using fluorescein-conjugated anti-FLAG antibody. Bacteria were visualized by labeling DNA with <t>Hoechst</t> <t>33288</t> (magenta). B) Western-blot detection of extracellular PilA_2. Bacteria were vortexed to release pili which were precipitated from supernatants. PilA_2-FLAG was detected in supernatant and whole cell lysates (pellet) using Anti-FLAG antibodies. C) DNA uptake assay of the reconstructed mutants defective for natural transformation. The ability of the transformation-deficient mutants were tested for the ability to internalize pGEM-HYG1, a non-replicative plasmid. Following incubation with the DNA and subsequent DNAse I treatment, the internalized DNA was detected in cells by PCR for pGEM-HYG. As control, chromosomal DNA was also detected by PCR. This multiplex PCR was analyzed by agarose gel electrophoresis and labeling of DNA with ethidium bromide.
    Hoechst 33288, supplied by Dapco Industries, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288/product/Dapco Industries
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1) Product Images from "Transposon-insertion sequencing in a clinical isolate of Legionella pneumophila identifies essential genes and determinants of natural transformation"

    Article Title: Transposon-insertion sequencing in a clinical isolate of Legionella pneumophila identifies essential genes and determinants of natural transformation

    Journal: bioRxiv

    doi: 10.1101/2020.10.01.323287

    PilA_2 assembly into extracellular filaments depends on pilE , the operon lpp1976-8 and pilZ . A) Visualization of PilA_2-FLAG filaments (green) by immunofluorescence microscopy using fluorescein-conjugated anti-FLAG antibody. Bacteria were visualized by labeling DNA with Hoechst 33288 (magenta). B) Western-blot detection of extracellular PilA_2. Bacteria were vortexed to release pili which were precipitated from supernatants. PilA_2-FLAG was detected in supernatant and whole cell lysates (pellet) using Anti-FLAG antibodies. C) DNA uptake assay of the reconstructed mutants defective for natural transformation. The ability of the transformation-deficient mutants were tested for the ability to internalize pGEM-HYG1, a non-replicative plasmid. Following incubation with the DNA and subsequent DNAse I treatment, the internalized DNA was detected in cells by PCR for pGEM-HYG. As control, chromosomal DNA was also detected by PCR. This multiplex PCR was analyzed by agarose gel electrophoresis and labeling of DNA with ethidium bromide.
    Figure Legend Snippet: PilA_2 assembly into extracellular filaments depends on pilE , the operon lpp1976-8 and pilZ . A) Visualization of PilA_2-FLAG filaments (green) by immunofluorescence microscopy using fluorescein-conjugated anti-FLAG antibody. Bacteria were visualized by labeling DNA with Hoechst 33288 (magenta). B) Western-blot detection of extracellular PilA_2. Bacteria were vortexed to release pili which were precipitated from supernatants. PilA_2-FLAG was detected in supernatant and whole cell lysates (pellet) using Anti-FLAG antibodies. C) DNA uptake assay of the reconstructed mutants defective for natural transformation. The ability of the transformation-deficient mutants were tested for the ability to internalize pGEM-HYG1, a non-replicative plasmid. Following incubation with the DNA and subsequent DNAse I treatment, the internalized DNA was detected in cells by PCR for pGEM-HYG. As control, chromosomal DNA was also detected by PCR. This multiplex PCR was analyzed by agarose gel electrophoresis and labeling of DNA with ethidium bromide.

    Techniques Used: Immunofluorescence, Microscopy, Labeling, Western Blot, Transformation Assay, Plasmid Preparation, Incubation, Multiplex Assay, Agarose Gel Electrophoresis

    hoechst 33288 dye  (Olympus)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Olympus hoechst 33288 dye
    Hoechst 33288 Dye, supplied by Olympus, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288 dye/product/Olympus
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 dye - by Bioz Stars, 2024-10
    86/100 stars

    Images

    hoechst 33288 dye  (Olympus)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Olympus hoechst 33288 dye
    Hoechst 33288 Dye, supplied by Olympus, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288 dye/product/Olympus
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 dye - by Bioz Stars, 2024-10
    86/100 stars

    Images

    hoechst 33288 dye  (Olympus)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Olympus hoechst 33288 dye
    Hoechst 33288 Dye, supplied by Olympus, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288 dye/product/Olympus
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 dye - by Bioz Stars, 2024-10
    86/100 stars

    Images

    hoechst 33288 dye  (Carl Zeiss)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Carl Zeiss hoechst 33288 dye
    Hoechst 33288 Dye, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288 dye/product/Carl Zeiss
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 dye - by Bioz Stars, 2024-10
    86/100 stars

    Images

    hoechst 33288 dye  (Carl Zeiss)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Carl Zeiss hoechst 33288 dye
    Hoechst 33288 Dye, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288 dye/product/Carl Zeiss
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 dye - by Bioz Stars, 2024-10
    86/100 stars

    Images

    hoechst 33288 dye  (Carl Zeiss)

     
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Carl Zeiss hoechst 33288 dye
    Hoechst 33288 Dye, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288 dye/product/Carl Zeiss
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 dye - by Bioz Stars, 2024-10
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • hoechst 33288  (Dapco Industries)
    86
    Dapco Industries hoechst 33288
    Hoechst 33288, supplied by Dapco Industries, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288/product/Dapco Industries
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier
    hoechst 33288 dye  (Olympus)
    86
    Olympus hoechst 33288 dye
    Hoechst 33288 Dye, supplied by Olympus, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288 dye/product/Olympus
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 dye - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier
    hoechst 33288 dye  (Carl Zeiss)
    86
    Carl Zeiss hoechst 33288 dye
    Hoechst 33288 Dye, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33288 dye/product/Carl Zeiss
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hoechst 33288 dye - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results