histone purification mini kit  (Active Motif)

 
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    Name:
    ChIP IT High Sensitivity Kit
    Description:
    The ChIP IT High Sensitivity HS Kit is the most sensitive ChIP kit available on the market It is designed to allow high quality DNA enrichment from as little as 1 000 cells per ChIP reaction for abundant targets The kit is also optimized to give superior results when performing chromatin immunoprecipitation using antibodies against low abundance proteins such as transcription factors or antibodies with sub optimal binding affinities To see how these features compare to the other ChIP Kits we offer see our ChIP Kit Selection Guide
    Catalog Number:
    53040
    Price:
    550
    Category:
    ChIP Assays
    Format:
    16 rxns
    Buy from Supplier


    Structured Review

    Active Motif histone purification mini kit
    The ChIP IT High Sensitivity HS Kit is the most sensitive ChIP kit available on the market It is designed to allow high quality DNA enrichment from as little as 1 000 cells per ChIP reaction for abundant targets The kit is also optimized to give superior results when performing chromatin immunoprecipitation using antibodies against low abundance proteins such as transcription factors or antibodies with sub optimal binding affinities To see how these features compare to the other ChIP Kits we offer see our ChIP Kit Selection Guide
    https://www.bioz.com/result/histone purification mini kit/product/Active Motif
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    histone purification mini kit - by Bioz Stars, 2021-07
    95/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. ChIP was performed on 1×107 MYCN3 neuroblastoma cells using the ChIP-IT Express Chromatin Immunoprecipitation Kit (Active Motif) according to the manufacturer’s instructions. .. Samples were cultured in one of four conditions: condition A, DMSO; condition B, Nutlin-3a; condition C, doxycycline; and condition D, doxycycline and Nutlin-3a.

    Article Title: PPARδ-mediated mitochondrial rewiring of osteoblasts determines bone mass
    Article Snippet: .. Chromatin immunoprecipitation (ChIP) assays To verify the binding of PPARd on selected promoters, ChIP assays were performed according to manufacturer instructions using the ChIP-IT® Express Chromatin Immunoprecipitation Kit (#53008, Active Motif, CA, USA). .. 25 μg of sonicated chromatin extract were incubated with 3.4 µg specific antibodies against PPARdelta (#ab178866, Abcam, Cambridge, MA, USA) or normal rabbit IgG antibodies (#sc-2027, Santa Cruz Biotechnology, CA, USA).

    Article Title: Nardilysin controls intestinal tumorigenesis through HDAC1/p53–dependent transcriptional regulation
    Article Snippet: The lysates were sonicated by a Bioruptor (12 cycles; work 60 seconds and rest 60 seconds). .. ChIP was performed using a ChIP-IT Express Chromatin Immunoprecipitation Kit (Active Motif). .. In brief, chromatin-bound proteins were immunoprecipitated with antibodies against NRDC (clone 2E6), p53 (2524, Cell Signaling Technology), HDAC1 (sc-81598, Santa Cruz Biotechnology), or control IgG (sc-2025, Santa Cruz Biotechnology).

    Article Title: HDAC1‐mSin3a‐NCOR1, Dnmt3b‐HDAC1‐Egr1 and Dnmt1‐PCNA‐UHRF1‐G9a regulate the NY‐ESO1 gene expression), HDAC1‐mSin3a‐NCOR1, Dnmt3b‐HDAC1‐Egr1 and Dnmt1‐PCNA‐UHRF1‐G9a regulate the NY‐ESO1 gene expression
    Article Snippet: Methylated DNA ImmunoPrecipitation experiments were realized by using MeDIP kit (Diagenode, France). .. ChIP and re‐ChIP assays were performed by using the ChIP‐IT™ chromatin immunoprecipitation Kits and the re‐ChIP‐IT™ Kit (Active Motif, France), with indicated antibodies and primers. .. Positive controls to show the ChIP validity realized with the Dnmt3a, Sirt1, Sirt2, SetDB1, HDAC2, HDAC6, HDAC7, myc, Sp1, MBD1, MBD2, MDB, MeCP2, EZH2, KDM6A and p300 antibodies were illustrated in .

    Article Title: Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells
    Article Snippet: The membranes were subsequently incubated with HRP-conjugated goat anti-rabbit secondary antibody (DAKO) and bands visualised using an EZ-ECL chemiluminescence detection kit (Geneflow, Staffordshire, UK) and ImageQuant developer (GE healthcare, Buckingamshire, UK). .. 2.8 Chromatin Immunoprecipitation Chromatin immunoprecipitation (ChIP) was performed according to the manufacturer’s guidelines (Active Motif; 53040). ..

    Article Title: Expression of Micro-RNA-492 (MiR-492) in Human Cervical Cancer Cell Lines is Upregulated by Transfection with Wild-Type P53, Irradiation, and 5-Fluorouracil Treatment In Vitro
    Article Snippet: Protein expression was quantified by using a Gel EDAS 290 Analysis System (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA) and Gel-Pro Analyzer 3.1 software (Media Cybernetics, Silver Spring, MD, USA). .. Chromatin immunoprecipitation (ChIP) assay Chromatin immunoprecipitation (ChIP) assay was performed by using ChIP-IT Express Chromatin Immunoprecipitation Kits (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. After cell fixation and chromatin shearing, 2 μg of anti-p53 (DO-1, 1: 1000, Santa Cruz) antibody or a monoclonal mouse IgG (Active Motif, Carlsbad, CA, USA) was mixed with chromatin solutions and incubated at 4°C overnight on an end-to-end rotator.

    Article Title: mTORC2 promotes cell survival through c-Myc–dependent up-regulation of E2F1
    Article Snippet: FTY720 (fingolimod) was obtained from Selleck. .. The Dual-Luciferase Reporter Assay kit was acquired from Promega, and the ChIP-IT Express Enzymatic Chromatin Immunoprecipitation kit from Active Motif. .. The PP2A Immunoprecipitation Phosphatase Assay kit was purchased from EMD Millipore.

    Article Title: ZNF503/Zpo2 drives aggressive breast cancer progression by down-regulation of GATA3 expression
    Article Snippet: For ChIP analysis using endogenous ZPO2 and ZBTB32 proteins, EpH4.9 cells were grown to near-confluency in a 150-mm culture dish. .. ChIP analysis was performed using the ChIP-IT High-Sensitivity Kit (Active Motif) following the manufacturer’s protocol. .. ChIP sample quality control was maintained with the ChIP-It Control qPCR Kit (Active Motif) and ChIP-It qPCR Analysis Kit (Active Motif).

    Binding Assay:

    Article Title: PPARδ-mediated mitochondrial rewiring of osteoblasts determines bone mass
    Article Snippet: .. Chromatin immunoprecipitation (ChIP) assays To verify the binding of PPARd on selected promoters, ChIP assays were performed according to manufacturer instructions using the ChIP-IT® Express Chromatin Immunoprecipitation Kit (#53008, Active Motif, CA, USA). .. 25 μg of sonicated chromatin extract were incubated with 3.4 µg specific antibodies against PPARdelta (#ab178866, Abcam, Cambridge, MA, USA) or normal rabbit IgG antibodies (#sc-2027, Santa Cruz Biotechnology, CA, USA).

    Reporter Assay:

    Article Title: mTORC2 promotes cell survival through c-Myc–dependent up-regulation of E2F1
    Article Snippet: FTY720 (fingolimod) was obtained from Selleck. .. The Dual-Luciferase Reporter Assay kit was acquired from Promega, and the ChIP-IT Express Enzymatic Chromatin Immunoprecipitation kit from Active Motif. .. The PP2A Immunoprecipitation Phosphatase Assay kit was purchased from EMD Millipore.

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  • 95
    Active Motif histone purification mini kit
    Histone Purification Mini Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/histone purification mini kit/product/Active Motif
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    histone purification mini kit - by Bioz Stars, 2021-07
    95/100 stars
      Buy from Supplier

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