histone h3 mouse monoclonal  (Sino Biological)


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  • 80
    Name:
    Histone H1 cDNA ORF Clone Mouse N GFPSpark tag
    Description:
    Full length Clone DNA of Mouse H1 histone family member 0 with N terminal GFPSpark tag
    Catalog Number:
    MG51008-ANG
    Price:
    225.0
    Category:
    cDNA Clone
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Product Aliases:
    D130017D06Rik cDNA ORF Clone Mouse, H1(0) cDNA ORF Clone Mouse, H1fv cDNA ORF Clone Mouse
    Molecule Name:
    H1F0,H1fv,Histone H1,
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    Structured Review

    Sino Biological histone h3 mouse monoclonal
    Histone H1 cDNA ORF Clone Mouse N GFPSpark tag
    Full length Clone DNA of Mouse H1 histone family member 0 with N terminal GFPSpark tag
    https://www.bioz.com/result/histone h3 mouse monoclonal/product/Sino Biological
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    histone h3 mouse monoclonal - by Bioz Stars, 2021-05
    80/100 stars

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    Related Articles

    Incubation:

    Article Title: Improving the specific antitumor efficacy of ONC by fusion with N-terminal domain of transferrin.
    Article Snippet: .. Hundred microliter (0.1 μg/mL) TfR with His-tag (Sino Biological) were added to the wells and incubated for 1 h. The plate was washed three times with PBST and 100 μL anti-His mouse antibody (1:500 dilution, R) was added and incubated for another hour. .. After the plate was washed three times with PBST, 100 μL goat anti-mouse IgG-HRP antibody (1:7500 dilution, R) was added and incubated for 1 h. A total of 100 μL TMB solution (Solarbio, China) was added into the plate after washing the plate five times with PBST.

    Article Title: Point mutation impairs centromeric CENH3 loading and induces haploid plants
    Article Snippet: Samples were electrotransferred onto Immobilon PVDF membranes (Millipore). .. Membranes were simultaneously incubated for 12 h at 4 °C in PBS and 5% low-fat milk with histone H3 mouse monoclonal (1:5,000; Sino Biological Inc., catalog no.100005-MM01-50) and A . thaliana CENH3 (HTR12) rabbit polyclonal (1:5,000; ab72001; Abcam) primary antibodies. .. Anti-mouse IRDye 680RD (1:5,000; LI-COR) and anti-rabbit IRDye 800CW (1:5,000; LI-COR) were used as secondary antibodies.

    Imaging:

    Article Title: Collagen triple helix repeat containing 1 is a new promigratory marker of arthritic pannus
    Article Snippet: Time-lapse microscopy Murine NIH 3T3 fibroblasts were plated on collagen type I-coated μ-slides (IBIDI USA Inc., Madison, WI) and incubated for 4 hours at 37 °C in 5 % CO2/95 % air in complete DMEM media supplemented with 10 % FBS to insure complete cell adhesion on a bridge. .. Subsequently, rhCTHRC1 protein (Sino Biological Inc., Beijing, China) was added at 1000 ng/ml, and imaging has been performed for 14 hours in a serum-containing medium at 37 °C using time-lapse phase contrast microscopy with a Cell Observer microscope (Carl Zeiss Microscopy GmbH, Göttingen, Germany). .. Recorded stacks of images were transferred to ImageJ [ ] and further analyzed with the MTrackJ plugin and Chemotaxis Tool (IBIDI USA Inc., Madison, WI, USA).

    Microscopy:

    Article Title: Collagen triple helix repeat containing 1 is a new promigratory marker of arthritic pannus
    Article Snippet: Time-lapse microscopy Murine NIH 3T3 fibroblasts were plated on collagen type I-coated μ-slides (IBIDI USA Inc., Madison, WI) and incubated for 4 hours at 37 °C in 5 % CO2/95 % air in complete DMEM media supplemented with 10 % FBS to insure complete cell adhesion on a bridge. .. Subsequently, rhCTHRC1 protein (Sino Biological Inc., Beijing, China) was added at 1000 ng/ml, and imaging has been performed for 14 hours in a serum-containing medium at 37 °C using time-lapse phase contrast microscopy with a Cell Observer microscope (Carl Zeiss Microscopy GmbH, Göttingen, Germany). .. Recorded stacks of images were transferred to ImageJ [ ] and further analyzed with the MTrackJ plugin and Chemotaxis Tool (IBIDI USA Inc., Madison, WI, USA).

    Activity Assay:

    Article Title: Sequential cleavage of insulin receptor by calpain 2 and γ-secretase impairs insulin signalling.
    Article Snippet: In brief, HepG2 cells were seeded in 10 cm dishes and incubated with DMEM containing 5.5 or 16.5 mmol/l glucose and 1% FBS (vol./vol.) with or without 3 mmol/l metformin or 5 μmol/l DON for 72 h. After washing the cells with DMEM, 10 ml DMEM without serum was added to each dish for an additional 24 h. The cell media were centrifuged at 10,000g for 30 min to remove cells and debris. .. Calpain activity assay To measure the proteolytic activity of calpain 2, recombinant IR containing His-tag at the C-terminal of the IRβ ectodomain (Sino Biological, Beijing, People’s Republic of China) was used as a substrate. .. Recombinant calpain 2 (2 μg) (Merck-Millipore) was incubated with the substrate (1.5 μg) in 20 mmol/l Tris-HCl (pH 7.4), 1 mmol/l EGTA, with or without 3 mmol/l CaCl2, at 25°C for 20 min.

    Recombinant:

    Article Title: Sequential cleavage of insulin receptor by calpain 2 and γ-secretase impairs insulin signalling.
    Article Snippet: In brief, HepG2 cells were seeded in 10 cm dishes and incubated with DMEM containing 5.5 or 16.5 mmol/l glucose and 1% FBS (vol./vol.) with or without 3 mmol/l metformin or 5 μmol/l DON for 72 h. After washing the cells with DMEM, 10 ml DMEM without serum was added to each dish for an additional 24 h. The cell media were centrifuged at 10,000g for 30 min to remove cells and debris. .. Calpain activity assay To measure the proteolytic activity of calpain 2, recombinant IR containing His-tag at the C-terminal of the IRβ ectodomain (Sino Biological, Beijing, People’s Republic of China) was used as a substrate. .. Recombinant calpain 2 (2 μg) (Merck-Millipore) was incubated with the substrate (1.5 μg) in 20 mmol/l Tris-HCl (pH 7.4), 1 mmol/l EGTA, with or without 3 mmol/l CaCl2, at 25°C for 20 min.

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    Sino Biological antibody mouse anti histone h3 monoclonal antibody
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    histone h3 mouse monoclonal - by Bioz Stars, 2021-05
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