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Illumina Inc hiseq 100 bp single end sequencing
Hiseq 100 Bp Single End Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiseq 100 bp single end sequencing/product/Illumina Inc
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hiseq 100 bp single end sequencing - by Bioz Stars, 2020-02
79/100 stars

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Flow Cytometry:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: .. Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing. .. An additional lane was dedicated to sequencing PhiX control libraries to provide internal calibration and to optimize base calling.

Sequencing:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: .. Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing. .. An additional lane was dedicated to sequencing PhiX control libraries to provide internal calibration and to optimize base calling.

Article Title: Characterization of chromosomal architecture in Arabidopsis by chromosome conformation capture
Article Snippet: .. All replicates in the libraries were subsequently pooled, and used for Illumina HiSeq 100 bp single end sequencing. ..

Random Hexamer Labeling:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: Briefly, the mRNA was fragmented in the presence of divalent cations at 94°C, and subsequently converted into double stranded cDNA following the first- and second-strand cDNA synthesis using random hexamer primers. .. Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing.

RNA Extraction:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: Paragraph title: RNA extraction, construction of cDNA libraries and DNA sequencing ... Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing.

Purification:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: Next, the cDNA was PCR-amplified for 15 cycles, amplicons were purified (QIAquick PCR purification kit, Qiagen Inc., Valencia CA, USA), and the size and concentration of the cDNA libraries were determined on an Agilent 2100 bioanalyzer. .. Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing.

Produced:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing. .. The sequence data produced in this study can be accessed at NCBI’s Sequence Read Achieve with the accession number SRA048723.

Concentration Assay:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: Next, the cDNA was PCR-amplified for 15 cycles, amplicons were purified (QIAquick PCR purification kit, Qiagen Inc., Valencia CA, USA), and the size and concentration of the cDNA libraries were determined on an Agilent 2100 bioanalyzer. .. Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing.

Incubation:

Article Title: Characterization of chromosomal architecture in Arabidopsis by chromosome conformation capture
Article Snippet: The pellet was then resuspended in 32.5 μl RSB and incubated for 2 minutes at room temperature. .. All replicates in the libraries were subsequently pooled, and used for Illumina HiSeq 100 bp single end sequencing.

DNA Sequencing:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: Paragraph title: RNA extraction, construction of cDNA libraries and DNA sequencing ... Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing.

Polymerase Chain Reaction:

Article Title: Transcriptomic analysis of the oleaginous microalga Neochloris oleoabundans reveals metabolic insights into triacylglyceride accumulation
Article Snippet: Next, the cDNA was PCR-amplified for 15 cycles, amplicons were purified (QIAquick PCR purification kit, Qiagen Inc., Valencia CA, USA), and the size and concentration of the cDNA libraries were determined on an Agilent 2100 bioanalyzer. .. Each of the four cDNA libraries (two nitrogen deplete and two nitrogen replete) was layered on a separate Illumina flow cell and sequenced at the Yale University Center for Genome Analysis using Illumina HiSeq 100 bp single-end sequencing.

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    Illumina Inc hiseq 2000 platform
    Hiseq 2000 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3916 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 platform/product/Illumina Inc
    Average 99 stars, based on 3916 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 platform - by Bioz Stars, 2020-02
    99/100 stars
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