his trap ff crude column  (GE Healthcare)

 
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    Name:
    HisTrap FF Crude
    Description:

    Catalog Number:
    11000458
    Price:
    None
    Category:
    HisTrap FF Crude histidine tagged protein purification columns
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    Structured Review

    GE Healthcare his trap ff crude column

    https://www.bioz.com/result/his trap ff crude column/product/GE Healthcare
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    his trap ff crude column - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: A Mutation in CABP2, Expressed in Cochlear Hair Cells, Causes Autosomal-Recessive Hearing Impairment
    Article Snippet: .. After centrifugation at 21,280 g, the His6 -tagged proteins were affinity purified from the supernatant with a HisTrap FF crude column (GE Healthcare, Munich, Germany). .. The proteins were eluted from the column, and the His6-tag was cleaved by the incubation of the protein solution with 1 U thrombin per 1 ml protein solution overnight at 4°C.

    Protease Inhibitor:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: .. The bacteria were lysed in Buffer A (50 mM sodium dihydrogen phosphate pH 7.5, 0.2 M NaCl, 5 mM Imidazole, 1 × EDTA free protease inhibitor cocktail (Roche 04693132001) and 0.4 mM betamercaptoetanol) and purified on a crude HisTrap column (GE Healthcare 11-0004-58) using Buffer A and Buffer B (50 mM sodium dihydrogen phosphate pH 7.5, 0.2 M NaCl, 500 mM Imidazole, 1 × EDTA free protease inhibitor cocktail (Roche 04693132001) and 0.4 mM betamercaptoetanol). .. Fractions with the highest optical density at 280 nm were pooled, dialyzed to Buffer C (20 mM Tris-HCl, pH 8) and further purified by ion exchange chromatography (Resource Q, GE Healthcare 520348) at pH 8 with buffer C and buffer D (20 mM Tris-HCl pH 8, 1 M NaCl).

    Labeling:

    Article Title: BIAM switch assay coupled to mass spectrometry identifies novel redox targets of NADPH oxidase 4
    Article Snippet: .. BIAM labeled proteins were loaded on HisTrap™ FF crude columns packed with Ni-Sepharose (from GE Healthcare) and purified using the Äkta FPLC from GE Healthcare. .. Purified protein was eluted with an 20–500 mM imidazole (from SigmaAldrich) gradient over 20 min with a flow rate of 1 ml/min.

    Purification:

    Article Title: G-patch domain and KOW motifs-containing protein, GPKOW; a nuclear RNA-binding protein regulated by protein kinase A
    Article Snippet: .. The bacteria were lysed in Buffer A (50 mM sodium dihydrogen phosphate pH 7.5, 0.2 M NaCl, 5 mM Imidazole, 1 × EDTA free protease inhibitor cocktail (Roche 04693132001) and 0.4 mM betamercaptoetanol) and purified on a crude HisTrap column (GE Healthcare 11-0004-58) using Buffer A and Buffer B (50 mM sodium dihydrogen phosphate pH 7.5, 0.2 M NaCl, 500 mM Imidazole, 1 × EDTA free protease inhibitor cocktail (Roche 04693132001) and 0.4 mM betamercaptoetanol). .. Fractions with the highest optical density at 280 nm were pooled, dialyzed to Buffer C (20 mM Tris-HCl, pH 8) and further purified by ion exchange chromatography (Resource Q, GE Healthcare 520348) at pH 8 with buffer C and buffer D (20 mM Tris-HCl pH 8, 1 M NaCl).

    Article Title: Purification and Characterization of Native and Vaccine Candidate Mutant Enterotoxigenic Escherichia coli Heat-Stable Toxins
    Article Snippet: .. The cleared lysate was purified with Ni-NTA using a HisTrap FF Crude 5 mL column (GE healthcare life sciences, Chicago, IL, USA) connected to an ÄKTA pure system (GE healthcare life sciences). ..

    Article Title: BIAM switch assay coupled to mass spectrometry identifies novel redox targets of NADPH oxidase 4
    Article Snippet: .. BIAM labeled proteins were loaded on HisTrap™ FF crude columns packed with Ni-Sepharose (from GE Healthcare) and purified using the Äkta FPLC from GE Healthcare. .. Purified protein was eluted with an 20–500 mM imidazole (from SigmaAldrich) gradient over 20 min with a flow rate of 1 ml/min.

    Protein Purification:

    Article Title: Synthesis of α,β-unsaturated esters via a chemo-enzymatic chain elongation approach by combining carboxylic acid reduction and Wittig reaction
    Article Snippet: .. Protein purification was performed on a HisTrapTM FF crude column (GE Healthcare, Piscataway, USA), and the protein was desorbed with an elution buffer (20 mM sodium phosphate, 0.5 M NaCl, 0.5 M imidazole, pH 7.4). .. The purified proteins His-CAR or His-PPTase were dialyzed in a sodium phosphate buffer (50 mM, pH 7.5) and then stored at −20 °C for further use.

    Fast Protein Liquid Chromatography:

    Article Title: BIAM switch assay coupled to mass spectrometry identifies novel redox targets of NADPH oxidase 4
    Article Snippet: .. BIAM labeled proteins were loaded on HisTrap™ FF crude columns packed with Ni-Sepharose (from GE Healthcare) and purified using the Äkta FPLC from GE Healthcare. .. Purified protein was eluted with an 20–500 mM imidazole (from SigmaAldrich) gradient over 20 min with a flow rate of 1 ml/min.

    Affinity Purification:

    Article Title: A Mutation in CABP2, Expressed in Cochlear Hair Cells, Causes Autosomal-Recessive Hearing Impairment
    Article Snippet: .. After centrifugation at 21,280 g, the His6 -tagged proteins were affinity purified from the supernatant with a HisTrap FF crude column (GE Healthcare, Munich, Germany). .. The proteins were eluted from the column, and the His6-tag was cleaved by the incubation of the protein solution with 1 U thrombin per 1 ml protein solution overnight at 4°C.

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  • 99
    GE Healthcare his trap ff crude column
    His Trap Ff Crude Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/his trap ff crude column/product/GE Healthcare
    Average 99 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    his trap ff crude column - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    GE Healthcare trap ff crude column
    Trap Ff Crude Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trap ff crude column/product/GE Healthcare
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    trap ff crude column - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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