hindiii xhoi simian virus 40 sv40  (Thermo Fisher)


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  • 99
    Name:
    HindIII 10 U µL
    Description:
    5 A ↓A G C T T 3 3 T T C G A ↑A 5 Thermo Scientific HindIII restriction enzyme recognizes A AGCTT sites and cuts best at 37°C in R buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0501
    Price:
    None
    Applications:
    Cloning|Restriction Enzyme Cloning
    Category:
    Proteins Enzymes Peptides
    Buy from Supplier


    Structured Review

    Thermo Fisher hindiii xhoi simian virus 40 sv40
    5 A ↓A G C T T 3 3 T T C G A ↑A 5 Thermo Scientific HindIII restriction enzyme recognizes A AGCTT sites and cuts best at 37°C in R buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/hindiii xhoi simian virus 40 sv40/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hindiii xhoi simian virus 40 sv40 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Modulation of TLR2 Protein Expression by miR-105 in Human Oral Keratinocytes *
    Article Snippet: .. The annealed oligonucleotides were digested with SpeI and HindIII and ligated into the multiple cloning site of the pMIRREPORT Luciferase vector (Ambion, Inc.). .. The post-transcriptional regulation of pMIRREPORT luciferase vector was potentially regulated by miRNA interactions with the TLR-2 3′-UTR.

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Luciferase:

    Article Title: Modulation of TLR2 Protein Expression by miR-105 in Human Oral Keratinocytes *
    Article Snippet: .. The annealed oligonucleotides were digested with SpeI and HindIII and ligated into the multiple cloning site of the pMIRREPORT Luciferase vector (Ambion, Inc.). .. The post-transcriptional regulation of pMIRREPORT luciferase vector was potentially regulated by miRNA interactions with the TLR-2 3′-UTR.

    Agarose Gel Electrophoresis:

    Article Title: Different nucleosomal architectures at early and late replicating origins in Saccharomyces cerevisiae
    Article Snippet: .. For each sample, 2.5 μg of DNA were digested with HindIII and PvuII enzymes (Fermentas), separated by electrophoresis in a 1.5% agarose gel, blotted and hybridized to an specific end-terminal probe. ..

    Southern Blot:

    Article Title: Single-molecule sequencing resolves the detailed structure of complex satellite DNA loci in Drosophila melanogaster
    Article Snippet: .. To confirm the locus orientation, we designed PCR primers that could only amplify an ∼15-kb segment of the distal part of the locus found in the PBcR-BLASR assembly (primer pair 3, 5′-CATGTGTGAACAGTGTATTCTG-3′ and 5′-GGCAGGAGTATTAAATCGATCTTC-3′) ( A; ) and confirmed its organization using restriction enzyme digestion with HindIII, EagI, SstI, and XmaI, and Southern blot analysis using a biotinylated Rsp probe and the North2South kit (ThermoFisher #17175) ( A). .. We validated the distal and proximal ends of the locus with a Southern blot analysis (see below) on genomic DNA digested with AccI, EcoRI, FspI, and SstI ( B; ).

    Ligation:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Article Title: Alterations of specific chromatin conformation affect ATRA-induced leukemia cell differentiation
    Article Snippet: .. HindIII digestion was performed at 37 °C overnight, and then, a proximity ligation was performed at 4 °C for 4 h. After reversing the cross-linking with a Proteinase K (Ambion, USA) treatment, the DNA was purified using a phenol–chloroform (Solarbio) extraction with ethanol precipitation. ..

    Ethanol Precipitation:

    Article Title: Alterations of specific chromatin conformation affect ATRA-induced leukemia cell differentiation
    Article Snippet: .. HindIII digestion was performed at 37 °C overnight, and then, a proximity ligation was performed at 4 °C for 4 h. After reversing the cross-linking with a Proteinase K (Ambion, USA) treatment, the DNA was purified using a phenol–chloroform (Solarbio) extraction with ethanol precipitation. ..

    Construct:

    Article Title: Flip-Flop HSV-BAC: bacterial artificial chromosome based system for rapid generation of recombinant herpes simplex virus vectors using two independent site-specific recombinases
    Article Snippet: .. Then the FRT sequence oligomers described above were subcloned into the BamHI/EcoRI sites of pUC-loxP to create pUC-LF, which was subsequently digested with HindIII and NdeI, filled-in and self-ligated to create pUC-LF2. pCMV-LacZV was constructed from pcDNA6-E/Uni-lacZ (Invitrogen) by AgeI and PmlI digestion followed by self-ligation. ..

    Purification:

    Article Title: Alterations of specific chromatin conformation affect ATRA-induced leukemia cell differentiation
    Article Snippet: .. HindIII digestion was performed at 37 °C overnight, and then, a proximity ligation was performed at 4 °C for 4 h. After reversing the cross-linking with a Proteinase K (Ambion, USA) treatment, the DNA was purified using a phenol–chloroform (Solarbio) extraction with ethanol precipitation. ..

    Electrophoresis:

    Article Title: Different nucleosomal architectures at early and late replicating origins in Saccharomyces cerevisiae
    Article Snippet: .. For each sample, 2.5 μg of DNA were digested with HindIII and PvuII enzymes (Fermentas), separated by electrophoresis in a 1.5% agarose gel, blotted and hybridized to an specific end-terminal probe. ..

    Polymerase Chain Reaction:

    Article Title: Genes A27L and F13L as Genetic Markers for the Isolation of Recombinant Vaccinia Virus
    Article Snippet: .. This PCR fragment was digested with HindIII and inserted into the unique HindIII site of plasmid pcDNA5/FRT/TO (Invitrogen). .. The final plasmid construct was used to generate a 293T-derived cell line inducibly expressing Cre, following the instructions of the Flp-In T-Rex system (Invitrogen).

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Article Title: Single-molecule sequencing resolves the detailed structure of complex satellite DNA loci in Drosophila melanogaster
    Article Snippet: .. To confirm the locus orientation, we designed PCR primers that could only amplify an ∼15-kb segment of the distal part of the locus found in the PBcR-BLASR assembly (primer pair 3, 5′-CATGTGTGAACAGTGTATTCTG-3′ and 5′-GGCAGGAGTATTAAATCGATCTTC-3′) ( A; ) and confirmed its organization using restriction enzyme digestion with HindIII, EagI, SstI, and XmaI, and Southern blot analysis using a biotinylated Rsp probe and the North2South kit (ThermoFisher #17175) ( A). .. We validated the distal and proximal ends of the locus with a Southern blot analysis (see below) on genomic DNA digested with AccI, EcoRI, FspI, and SstI ( B; ).

    Expressing:

    Article Title: A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines
    Article Snippet: .. Molecular biology: the pGS[#P’#O(LA)-mEmerald] and pAGOC{#P’#O(LA)-mEmerald} vectors A hybrid sequence, consisting of (i) a HindIII site, (ii) the modular fluorescent protein expression cassette as described above and (iii) a XbaI site, was de novo synthetized and inserted into the unique SfiI site of pMK-RQ (Thermo Fisher Scientific). .. The resulting vector was termed pGS[#P’#O(LA)-mEmerald].

    Sequencing:

    Article Title: Flip-Flop HSV-BAC: bacterial artificial chromosome based system for rapid generation of recombinant herpes simplex virus vectors using two independent site-specific recombinases
    Article Snippet: .. Then the FRT sequence oligomers described above were subcloned into the BamHI/EcoRI sites of pUC-loxP to create pUC-LF, which was subsequently digested with HindIII and NdeI, filled-in and self-ligated to create pUC-LF2. pCMV-LacZV was constructed from pcDNA6-E/Uni-lacZ (Invitrogen) by AgeI and PmlI digestion followed by self-ligation. ..

    Article Title: A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines
    Article Snippet: .. Molecular biology: the pGS[#P’#O(LA)-mEmerald] and pAGOC{#P’#O(LA)-mEmerald} vectors A hybrid sequence, consisting of (i) a HindIII site, (ii) the modular fluorescent protein expression cassette as described above and (iii) a XbaI site, was de novo synthetized and inserted into the unique SfiI site of pMK-RQ (Thermo Fisher Scientific). .. The resulting vector was termed pGS[#P’#O(LA)-mEmerald].

    Plasmid Preparation:

    Article Title: Modulation of TLR2 Protein Expression by miR-105 in Human Oral Keratinocytes *
    Article Snippet: .. The annealed oligonucleotides were digested with SpeI and HindIII and ligated into the multiple cloning site of the pMIRREPORT Luciferase vector (Ambion, Inc.). .. The post-transcriptional regulation of pMIRREPORT luciferase vector was potentially regulated by miRNA interactions with the TLR-2 3′-UTR.

    Article Title: Genes A27L and F13L as Genetic Markers for the Isolation of Recombinant Vaccinia Virus
    Article Snippet: .. This PCR fragment was digested with HindIII and inserted into the unique HindIII site of plasmid pcDNA5/FRT/TO (Invitrogen). .. The final plasmid construct was used to generate a 293T-derived cell line inducibly expressing Cre, following the instructions of the Flp-In T-Rex system (Invitrogen).

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).