hindiii xbai sites  (Thermo Fisher)


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    Name:
    HindIII 10 U µL
    Description:
    5 A ↓A G C T T 3 3 T T C G A ↑A 5 Thermo Scientific HindIII restriction enzyme recognizes A AGCTT sites and cuts best at 37°C in R buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0501
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Cloning|Restriction Enzyme Cloning
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    Structured Review

    Thermo Fisher hindiii xbai sites
    5 A ↓A G C T T 3 3 T T C G A ↑A 5 Thermo Scientific HindIII restriction enzyme recognizes A AGCTT sites and cuts best at 37°C in R buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/hindiii xbai sites/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hindiii xbai sites - by Bioz Stars, 2021-03
    96/100 stars

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    Purification:

    Article Title: Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification
    Article Snippet: .. 1 µg samples of the purified HindIII-digested DNA were re-circularized in a 200 µl reaction volume with 25 U T4 DNA Ligase (Fermentas, St. Leon-Rot, Germany) as described earlier with VH gene mutagenesis. .. Ligations were purified with Qiagen PCR purification Kit, eluted in 50 µl Tris-HCl (pH 8.5) and DNA concentrations were determined with Nanodrop (Thermo Fisher Scientific, Waltham, USA).

    Article Title: Alterations of specific chromatin conformation affect ATRA-induced leukemia cell differentiation
    Article Snippet: Then, the nuclei were washed with 1× NEBuffer2 (NEB, England) and treated with 0.3% SDS at 65 °C. .. HindIII digestion was performed at 37 °C overnight, and then, a proximity ligation was performed at 4 °C for 4 h. After reversing the cross-linking with a Proteinase K (Ambion, USA) treatment, the DNA was purified using a phenol–chloroform (Solarbio) extraction with ethanol precipitation. ..

    Mutagenesis:

    Article Title: Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification
    Article Snippet: .. 1 µg samples of the purified HindIII-digested DNA were re-circularized in a 200 µl reaction volume with 25 U T4 DNA Ligase (Fermentas, St. Leon-Rot, Germany) as described earlier with VH gene mutagenesis. .. Ligations were purified with Qiagen PCR purification Kit, eluted in 50 µl Tris-HCl (pH 8.5) and DNA concentrations were determined with Nanodrop (Thermo Fisher Scientific, Waltham, USA).

    Ligation:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Article Title: Network analysis of promoter interactions reveals the hierarchical differences in genome organisation between human pluripotent states
    Article Snippet: Restriction digest was performed overnight at 37°C with agitation (950 rpm) with HindIII (NEB; 1500 units per 7 million cells). .. Using biotin-14-dATP (Life Technologies), dCTP, dGTP and dTTP (Life Technologies; all at a final concentration of 30 μM), the HindIII restriction sites were then filled in with Klenow (NEB) for 75 minutes at 37°C, followed by ligation for 4 hours at 16°C (50 units T4 DNA ligase (Life Technologies) per 7 million cells starting material) in a total volume of 5.5 mL ligation buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM DTT, 100 μg/mL BSA) per 7 million cells starting material. .. After ligation, crosslinking was reversed by incubation with Proteinase K (Roche; 65 μl of 10mg/mL per 7 million cells starting material) at 65°C overnight.

    Article Title: Alterations of specific chromatin conformation affect ATRA-induced leukemia cell differentiation
    Article Snippet: Then, the nuclei were washed with 1× NEBuffer2 (NEB, England) and treated with 0.3% SDS at 65 °C. .. HindIII digestion was performed at 37 °C overnight, and then, a proximity ligation was performed at 4 °C for 4 h. After reversing the cross-linking with a Proteinase K (Ambion, USA) treatment, the DNA was purified using a phenol–chloroform (Solarbio) extraction with ethanol precipitation. ..

    Polymerase Chain Reaction:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Article Title: Allelic Variations of Plasmodium vivax Apical Membrane Antigen-1 (Pv AMA-1) in Malarious Areas of Southeastern Iran Using PCR-RFLP Technique
    Article Snippet: The purified Pv AMA-1 gene was sent for sequencing after gel extraction processing using QIA quick Gel Extraction Kit. .. In order to determine the presence of different alleles of Pv AMA-1 gene in the region, PCR–RFLP technique was done to digest the gene using three restriction enzymes EcoR-1, Pvu-II and Hind3 (Thermo cat No #ER0271, #ER0631 and ferments cat No #ER0501 respectively) according to the manufacturer’s recommendations. .. The products were visualized under UV illumination after electrophoresis on 3% agarose gel containing ethidium bromide.

    Article Title: Allelic Variations of Plasmodium vivax Apical Membrane Antigen-1 (Pv AMA-1) in Malarious Areas of Southeastern Iran Using PCR-RFLP Technique
    Article Snippet: The purified Pv AMA-1 gene was sent for sequencing after gel extraction processing using QIA quick Gel Extraction Kit. .. RFLP In order to determine the presence of different alleles of Pv AMA-1 gene in the region, PCR–RFLP technique was done to digest the gene using three restriction enzymes EcoR-1, Pvu-II and Hind3 (Thermo cat No #ER0271, #ER0631 and ferments cat No #ER0501 respectively) according to the manufacturer’s recommendations. .. The products were visualized under UV illumination after electrophoresis on 3% agarose gel containing ethidium bromide.

    Plasmid Preparation:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Clone Assay:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Concentration Assay:

    Article Title: Network analysis of promoter interactions reveals the hierarchical differences in genome organisation between human pluripotent states
    Article Snippet: Restriction digest was performed overnight at 37°C with agitation (950 rpm) with HindIII (NEB; 1500 units per 7 million cells). .. Using biotin-14-dATP (Life Technologies), dCTP, dGTP and dTTP (Life Technologies; all at a final concentration of 30 μM), the HindIII restriction sites were then filled in with Klenow (NEB) for 75 minutes at 37°C, followed by ligation for 4 hours at 16°C (50 units T4 DNA ligase (Life Technologies) per 7 million cells starting material) in a total volume of 5.5 mL ligation buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP, 10 mM DTT, 100 μg/mL BSA) per 7 million cells starting material. .. After ligation, crosslinking was reversed by incubation with Proteinase K (Roche; 65 μl of 10mg/mL per 7 million cells starting material) at 65°C overnight.

    Sequencing:

    Article Title: A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines
    Article Snippet: The resulting vector was termed pAGOC and used (i) as a transformation-ready vector for germline transformation, and (ii) as an intermediate vector for further cloning operations. .. Molecular biology: the pGS[#P’#O(LA)-mEmerald] and pAGOC{#P’#O(LA)-mEmerald} vectorsA hybrid sequence, consisting of (i) a HindIII site, (ii) the modular fluorescent protein expression cassette as described above and (iii) a XbaI site, was de novo synthetized and inserted into the unique SfiI site of pMK-RQ (Thermo Fisher Scientific). .. The resulting vector was termed pGS[#P’#O(LA)-mEmerald].

    Expressing:

    Article Title: A universal vector concept for a direct genotyping of transgenic organisms and a systematic creation of homozygous lines
    Article Snippet: The resulting vector was termed pAGOC and used (i) as a transformation-ready vector for germline transformation, and (ii) as an intermediate vector for further cloning operations. .. Molecular biology: the pGS[#P’#O(LA)-mEmerald] and pAGOC{#P’#O(LA)-mEmerald} vectorsA hybrid sequence, consisting of (i) a HindIII site, (ii) the modular fluorescent protein expression cassette as described above and (iii) a XbaI site, was de novo synthetized and inserted into the unique SfiI site of pMK-RQ (Thermo Fisher Scientific). .. The resulting vector was termed pGS[#P’#O(LA)-mEmerald].

    Ethanol Precipitation:

    Article Title: Alterations of specific chromatin conformation affect ATRA-induced leukemia cell differentiation
    Article Snippet: Then, the nuclei were washed with 1× NEBuffer2 (NEB, England) and treated with 0.3% SDS at 65 °C. .. HindIII digestion was performed at 37 °C overnight, and then, a proximity ligation was performed at 4 °C for 4 h. After reversing the cross-linking with a Proteinase K (Ambion, USA) treatment, the DNA was purified using a phenol–chloroform (Solarbio) extraction with ethanol precipitation. ..

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  • 96
    Thermo Fisher hindiii xbai sites
    Hindiii Xbai Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hindiii xbai sites/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hindiii xbai sites - by Bioz Stars, 2021-03
    96/100 stars
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