Structured Review

Shimadzu Corporation astragaloside i
Polysaccharide and sucrose are only present in the water extract of Radix Astragali (RA) (AM-W). (a) LC-ELSD chromatograms of the AM-W (a) and the ethanolic extract (AM-E) (b) with a 70-min retention time. Peak identification: 1, astragaloside II; 2, isoastragaloside I; 3, astragaloside I. (b) LC-ELSD chromatograms of the AM-W (a) and sucrose (b) with a 10-min retention time. Peak identification: 1, 2, and 3, polysaccharides; peak 4, sucrose. (c) The representative  1 H NMR and  13 C NMR spectra of AM-W-F1 and AM-W-F2 are illustrated. (d) Confirmation of the hypoglycemic bioactivity of the AM-W. Insulin-dependent diabetic rats were generated with an initial average glucose level at 407.2 ± 17.8 mg/dL ( n  = 22). The hypoglycemic effect was evaluated by determining the percentage of the initial fasting glucose level in each group. Differences at the same time point were determined by ANOVA followed by the Tukey-Kramer test. * P
Astragaloside I, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 89/100, based on 896 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/astragaloside i/product/Shimadzu Corporation
Average 89 stars, based on 896 article reviews
Price from $9.99 to $1999.99
astragaloside i - by Bioz Stars, 2020-09
89/100 stars

Images

1) Product Images from "Administration of a Decoction of Sucrose- and Polysaccharide-Rich Radix Astragali (Huang Qi) Ameliorated Insulin Resistance and Fatty Liver but Affected Beta-Cell Function in Type 2 Diabetic Rats"

Article Title: Administration of a Decoction of Sucrose- and Polysaccharide-Rich Radix Astragali (Huang Qi) Ameliorated Insulin Resistance and Fatty Liver but Affected Beta-Cell Function in Type 2 Diabetic Rats

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2011/349807

Polysaccharide and sucrose are only present in the water extract of Radix Astragali (RA) (AM-W). (a) LC-ELSD chromatograms of the AM-W (a) and the ethanolic extract (AM-E) (b) with a 70-min retention time. Peak identification: 1, astragaloside II; 2, isoastragaloside I; 3, astragaloside I. (b) LC-ELSD chromatograms of the AM-W (a) and sucrose (b) with a 10-min retention time. Peak identification: 1, 2, and 3, polysaccharides; peak 4, sucrose. (c) The representative  1 H NMR and  13 C NMR spectra of AM-W-F1 and AM-W-F2 are illustrated. (d) Confirmation of the hypoglycemic bioactivity of the AM-W. Insulin-dependent diabetic rats were generated with an initial average glucose level at 407.2 ± 17.8 mg/dL ( n  = 22). The hypoglycemic effect was evaluated by determining the percentage of the initial fasting glucose level in each group. Differences at the same time point were determined by ANOVA followed by the Tukey-Kramer test. * P
Figure Legend Snippet: Polysaccharide and sucrose are only present in the water extract of Radix Astragali (RA) (AM-W). (a) LC-ELSD chromatograms of the AM-W (a) and the ethanolic extract (AM-E) (b) with a 70-min retention time. Peak identification: 1, astragaloside II; 2, isoastragaloside I; 3, astragaloside I. (b) LC-ELSD chromatograms of the AM-W (a) and sucrose (b) with a 10-min retention time. Peak identification: 1, 2, and 3, polysaccharides; peak 4, sucrose. (c) The representative 1 H NMR and 13 C NMR spectra of AM-W-F1 and AM-W-F2 are illustrated. (d) Confirmation of the hypoglycemic bioactivity of the AM-W. Insulin-dependent diabetic rats were generated with an initial average glucose level at 407.2 ± 17.8 mg/dL ( n = 22). The hypoglycemic effect was evaluated by determining the percentage of the initial fasting glucose level in each group. Differences at the same time point were determined by ANOVA followed by the Tukey-Kramer test. * P

Techniques Used: Nuclear Magnetic Resonance, Generated

2) Product Images from "Neurophysiological and Neurochemical Effects of the Putative Cognitive Enhancer (S)-CE-123 on Mesocorticolimbic Dopamine System"

Article Title: Neurophysiological and Neurochemical Effects of the Putative Cognitive Enhancer (S)-CE-123 on Mesocorticolimbic Dopamine System

Journal: Biomolecules

doi: 10.3390/biom10050779

Effect of ( S )-CE-123 and R -modafinil on the emission of 50-kHz ultrasonic vocalizations. Graphs showing the effects of ( S )-CE-123 (left) or R -modafinil (right) on the emission of 50-kHz USVs. The arrow indicates i.p. injection of ( S )-CE-123 or R -modafinil at the dose of 1 mg/kg (blue), 5 mg/kg (green), 10 mg/kg (red) or vehicle (black). Results are presented as means ± SEM of the absolute numbers of 50-kHz USVs emitted. ( S )-CE-123 or R -modafinil did not increase the numbers of 50-kHz ultrasonic calls emitted, as compared to vehicle. USVs = ultrasonic vocalizations, vehicle n = 7; ( S )-CE-123 n = 13; R -modafinil n = 13. Two-way ANOVA.
Figure Legend Snippet: Effect of ( S )-CE-123 and R -modafinil on the emission of 50-kHz ultrasonic vocalizations. Graphs showing the effects of ( S )-CE-123 (left) or R -modafinil (right) on the emission of 50-kHz USVs. The arrow indicates i.p. injection of ( S )-CE-123 or R -modafinil at the dose of 1 mg/kg (blue), 5 mg/kg (green), 10 mg/kg (red) or vehicle (black). Results are presented as means ± SEM of the absolute numbers of 50-kHz USVs emitted. ( S )-CE-123 or R -modafinil did not increase the numbers of 50-kHz ultrasonic calls emitted, as compared to vehicle. USVs = ultrasonic vocalizations, vehicle n = 7; ( S )-CE-123 n = 13; R -modafinil n = 13. Two-way ANOVA.

Techniques Used: Injection

Effect of ( S )-CE-123 and R -modafinil on in vivo electrical activity of putative dopamine cells. Representative firing rate histograms of putative ventral tegmental area (VTA) dopamine neurons ( A ) from rats that received cumulative intravenous injections of vehicle (left), ( S )-CE-123 (10 mg/kg; middle) or R -modafinil (10 mg/kg; right). Arrows indicate the time of injections and number of the dose (mg/kg). R -modafinil and ( S )-CE-123 did not change firing frequency ( B ) or bursting activity ( C ) of putative VTA dopamine neurons (vehicle n = 5; R -modafinil n = 6, ( S )-CE-123 n = 7). Symbols and bars represent means ± SEM, RM one-way ANOVA. ( D ) Histological brain section showing the recording site in the VTA. The black triangle indicates the pontamine sky blue dye. Abbreviations: Aq, aqueduct; RN, red nucleus; IP, interpeduncular nucleus; SN, substantia nigra; VTA, ventral tegmental area. Scale bar, 0.5 mm.
Figure Legend Snippet: Effect of ( S )-CE-123 and R -modafinil on in vivo electrical activity of putative dopamine cells. Representative firing rate histograms of putative ventral tegmental area (VTA) dopamine neurons ( A ) from rats that received cumulative intravenous injections of vehicle (left), ( S )-CE-123 (10 mg/kg; middle) or R -modafinil (10 mg/kg; right). Arrows indicate the time of injections and number of the dose (mg/kg). R -modafinil and ( S )-CE-123 did not change firing frequency ( B ) or bursting activity ( C ) of putative VTA dopamine neurons (vehicle n = 5; R -modafinil n = 6, ( S )-CE-123 n = 7). Symbols and bars represent means ± SEM, RM one-way ANOVA. ( D ) Histological brain section showing the recording site in the VTA. The black triangle indicates the pontamine sky blue dye. Abbreviations: Aq, aqueduct; RN, red nucleus; IP, interpeduncular nucleus; SN, substantia nigra; VTA, ventral tegmental area. Scale bar, 0.5 mm.

Techniques Used: In Vivo, Activity Assay

Effect of ( S )-CE-123 and R -modafinil on in vivo electrical activity of putative pyramidal cells. Representative firing rate histograms of putative pyramidal neurons ( A ) from rats that received cumulative intravenous injections of vehicle (left), ( S )-CE-123 (10 mg/kg; middle) or R -modafinil (10 mg/kg; right). Arrows indicate the time of injections and number of the dose (mg/kg). ( S )-CE-123 reduced firing frequency ( B ) in a dose-dependent manner (vehicle n = 5; R -modafinil n = 7, ( S )-CE-123 n = 7). Symbols and bars represent means ± SEM, * p
Figure Legend Snippet: Effect of ( S )-CE-123 and R -modafinil on in vivo electrical activity of putative pyramidal cells. Representative firing rate histograms of putative pyramidal neurons ( A ) from rats that received cumulative intravenous injections of vehicle (left), ( S )-CE-123 (10 mg/kg; middle) or R -modafinil (10 mg/kg; right). Arrows indicate the time of injections and number of the dose (mg/kg). ( S )-CE-123 reduced firing frequency ( B ) in a dose-dependent manner (vehicle n = 5; R -modafinil n = 7, ( S )-CE-123 n = 7). Symbols and bars represent means ± SEM, * p

Techniques Used: In Vivo, Activity Assay

Effect of ( S )-CE-123 and R -modafinil on dopamine transmission in the NAc shell. Graphs showing the effects of ( S )-CE-123 (left) or R -modafinil (right) on dopamine levels in the shell of the NAc. The arrow indicates i.p. injection of ( S )-CE-123 or R -modafinil at the dose of 1 mg/kg (blue), 5 mg/kg (green), 10 mg/kg (red) or vehicle (black). Results are presented as mean ± SEM of change in dopamine extracellular levels expressed as the percentage of basal values. Solid symbol: p
Figure Legend Snippet: Effect of ( S )-CE-123 and R -modafinil on dopamine transmission in the NAc shell. Graphs showing the effects of ( S )-CE-123 (left) or R -modafinil (right) on dopamine levels in the shell of the NAc. The arrow indicates i.p. injection of ( S )-CE-123 or R -modafinil at the dose of 1 mg/kg (blue), 5 mg/kg (green), 10 mg/kg (red) or vehicle (black). Results are presented as mean ± SEM of change in dopamine extracellular levels expressed as the percentage of basal values. Solid symbol: p

Techniques Used: Transmission Assay, Injection

Effect of ( S )-CE-123 and R -modafinil on dopamine transmission in the IL/PrL cortex. Graphs showing the effects of ( S )-CE-123 (left) or R -modafinil (right) on dopamine levels in the IL/PrL cortex. The arrow indicates i.p. injection of ( S )-CE-123 or R -modafinil at the dose of 1 mg/kg (blue), 5 mg/kg (green), 10 mg/kg (red) or vehicle (black). Results are presented as mean ± SEM of change in dopamine extracellular levels expressed as the percentage of basal values. Solid symbol: p
Figure Legend Snippet: Effect of ( S )-CE-123 and R -modafinil on dopamine transmission in the IL/PrL cortex. Graphs showing the effects of ( S )-CE-123 (left) or R -modafinil (right) on dopamine levels in the IL/PrL cortex. The arrow indicates i.p. injection of ( S )-CE-123 or R -modafinil at the dose of 1 mg/kg (blue), 5 mg/kg (green), 10 mg/kg (red) or vehicle (black). Results are presented as mean ± SEM of change in dopamine extracellular levels expressed as the percentage of basal values. Solid symbol: p

Techniques Used: Transmission Assay, Injection

3) Product Images from "Determination of Tangeretin in Rat Plasma: Assessment of Its Clearance and Absolute Oral Bioavailability"

Article Title: Determination of Tangeretin in Rat Plasma: Assessment of Its Clearance and Absolute Oral Bioavailability

Journal: Pharmaceutics

doi: 10.3390/pharmaceutics10010003

Representative chromatograms (UV absorbance, λ = 312 nm) of: ( a ) a blank plasma sample spiked with tangeretin (300 ng/mL) and trans -stilbene (internal standard) (900 ng/mL); ( b ) a pre-dosing plasma sample; ( c ) a post-dosing plasma sample collected from a Sprague—Dawley rat at 30 min after receiving an intravenous dose of tangeretin (10 mg/kg) (without internal standard); and ( d ) a post-dosing plasma sample collected from a Sprague—Dawley rat at 30 min after receiving an oral solution of tangeretin (50 mg/kg) (with internal standard). Peak 1: tangeretin, peak 2: trans -stilbene (internal standard), peaks 3 and 4: unidentified metabolites.
Figure Legend Snippet: Representative chromatograms (UV absorbance, λ = 312 nm) of: ( a ) a blank plasma sample spiked with tangeretin (300 ng/mL) and trans -stilbene (internal standard) (900 ng/mL); ( b ) a pre-dosing plasma sample; ( c ) a post-dosing plasma sample collected from a Sprague—Dawley rat at 30 min after receiving an intravenous dose of tangeretin (10 mg/kg) (without internal standard); and ( d ) a post-dosing plasma sample collected from a Sprague—Dawley rat at 30 min after receiving an oral solution of tangeretin (50 mg/kg) (with internal standard). Peak 1: tangeretin, peak 2: trans -stilbene (internal standard), peaks 3 and 4: unidentified metabolites.

Techniques Used:

4) Product Images from "Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes"

Article Title: Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes

Journal: Microbial Cell Factories

doi: 10.1186/s12934-016-0556-x

RP-HPLC ergosterol analysis from the parental host strain, Δ CYP61 (+, −) strain and Δ CYP61 (−, −) strain respectively, and astaxanthin production in each. a Cell concentration (OD 600 ); b intracellular astaxanthin content (mg/g-cell). c volumetric astaxanthin concentration (mg/L). Circle ( black ), triangle ( blue ) and square ( red ) symbols represent values of the parental host strain, Δ CYP61 (+, −) strain and Δ CYP61 (−, −) strain, respectively
Figure Legend Snippet: RP-HPLC ergosterol analysis from the parental host strain, Δ CYP61 (+, −) strain and Δ CYP61 (−, −) strain respectively, and astaxanthin production in each. a Cell concentration (OD 600 ); b intracellular astaxanthin content (mg/g-cell). c volumetric astaxanthin concentration (mg/L). Circle ( black ), triangle ( blue ) and square ( red ) symbols represent values of the parental host strain, Δ CYP61 (+, −) strain and Δ CYP61 (−, −) strain, respectively

Techniques Used: High Performance Liquid Chromatography, Concentration Assay

5) Product Images from "Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil"

Article Title: Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil

Journal: Molecules

doi: 10.3390/molecules171112560

HPLC/DAD phenolics profile of CE ( A ), CHCl 3 ( B ), AcOEt ( C ) and n-BuOH ( D ) fractions of S. guaraniticum leaves. (1) chlorogenic acid, (2) caffeic acid, (3) rosmarinic acid, ( * ) unknown peaks.
Figure Legend Snippet: HPLC/DAD phenolics profile of CE ( A ), CHCl 3 ( B ), AcOEt ( C ) and n-BuOH ( D ) fractions of S. guaraniticum leaves. (1) chlorogenic acid, (2) caffeic acid, (3) rosmarinic acid, ( * ) unknown peaks.

Techniques Used: High Performance Liquid Chromatography

6) Product Images from "Bactofencin A, a New Type of Cationic Bacteriocin with Unusual Immunity"

Article Title: Bactofencin A, a New Type of Cationic Bacteriocin with Unusual Immunity

Journal: mBio

doi: 10.1128/mBio.00498-13

HPLC profile (A), MALDI-TOF MS data (B), and antimicrobial activity (C) of purified bactofencin A. WVL, wavelength.
Figure Legend Snippet: HPLC profile (A), MALDI-TOF MS data (B), and antimicrobial activity (C) of purified bactofencin A. WVL, wavelength.

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Activity Assay, Purification

7) Product Images from "Clarithromycin-Loaded Poly (Lactic-co-glycolic Acid) (PLGA) Nanoparticles for Oral Administration: Effect of Polymer Molecular Weight and Surface Modification with Chitosan on Formulation, Nanoparticle Characterization and Antibacterial Effects"

Article Title: Clarithromycin-Loaded Poly (Lactic-co-glycolic Acid) (PLGA) Nanoparticles for Oral Administration: Effect of Polymer Molecular Weight and Surface Modification with Chitosan on Formulation, Nanoparticle Characterization and Antibacterial Effects

Journal: Polymers

doi: 10.3390/polym11101632

Thermal (DSC) analysis curves of CLR and NPs, ( a ) CLR, ( b ) 502H-Blank, ( c ) 503H-Blank, ( d ) 504H-Blank, ( e ) CS-502H-Blank, ( f ) CS-503H-Blank, ( g ) CS-504H-Blank, ( h ) 502H, ( i ) 503H, ( j ) 504H, ( k ) CS-502H, ( l ) CS-503H, ( m ) CS-504H.
Figure Legend Snippet: Thermal (DSC) analysis curves of CLR and NPs, ( a ) CLR, ( b ) 502H-Blank, ( c ) 503H-Blank, ( d ) 504H-Blank, ( e ) CS-502H-Blank, ( f ) CS-503H-Blank, ( g ) CS-504H-Blank, ( h ) 502H, ( i ) 503H, ( j ) 504H, ( k ) CS-502H, ( l ) CS-503H, ( m ) CS-504H.

Techniques Used:

In vitro release of pure CLR, CLR from PLGA NPs and CS-coated PLGA NPs at 37 °C in intestinal fluid phosphate buffer pH 6.8 supplemented with 1% Tween 80. ( a ) 144 hours release profile, ( b ) 24 hours release profile.
Figure Legend Snippet: In vitro release of pure CLR, CLR from PLGA NPs and CS-coated PLGA NPs at 37 °C in intestinal fluid phosphate buffer pH 6.8 supplemented with 1% Tween 80. ( a ) 144 hours release profile, ( b ) 24 hours release profile.

Techniques Used: In Vitro

FT-IR spectrum of CLR and NPs, ( a ) CLR, ( b ) 502H-Blank, ( c ) 503H-Blank, ( d ) 504H-Blank, ( e ) CS-502H-Blank, ( f ) CS-503H-Blank, ( g ) CS-504H-Blank, ( h ) 502H, ( i ) 503H, ( j ) 504H, ( k ) CS-502H, ( l ) CS-503H, ( m ) CS-504H.
Figure Legend Snippet: FT-IR spectrum of CLR and NPs, ( a ) CLR, ( b ) 502H-Blank, ( c ) 503H-Blank, ( d ) 504H-Blank, ( e ) CS-502H-Blank, ( f ) CS-503H-Blank, ( g ) CS-504H-Blank, ( h ) 502H, ( i ) 503H, ( j ) 504H, ( k ) CS-502H, ( l ) CS-503H, ( m ) CS-504H.

Techniques Used:

1 H-NMR spectrum of CLR and NPs, ( a ) CLR, ( b ) 502H-Blank, ( c ) 503H-Blank, ( d ) 504H-Blank, ( e ) CS-502H-Blank, ( f ) CS-503H-Blank, ( g ) CS-504H-Blank, ( h ) 502H, ( i ) 503H, ( j ) 504H, ( k ) CS-502H, ( l ) CS-503H, ( m ) CS-504H.
Figure Legend Snippet: 1 H-NMR spectrum of CLR and NPs, ( a ) CLR, ( b ) 502H-Blank, ( c ) 503H-Blank, ( d ) 504H-Blank, ( e ) CS-502H-Blank, ( f ) CS-503H-Blank, ( g ) CS-504H-Blank, ( h ) 502H, ( i ) 503H, ( j ) 504H, ( k ) CS-502H, ( l ) CS-503H, ( m ) CS-504H.

Techniques Used: Nuclear Magnetic Resonance

8) Product Images from "An Efficient Method for the Preparative Isolation and Purification of Flavonoid Glycosides and Caffeoylquinic Acid Derivatives from Leaves of Lonicera japonica Thunb. Using High Speed Counter-Current Chromatography (HSCCC) and Prep-HPLC Guided by DPPH-HPLC Experiments"

Article Title: An Efficient Method for the Preparative Isolation and Purification of Flavonoid Glycosides and Caffeoylquinic Acid Derivatives from Leaves of Lonicera japonica Thunb. Using High Speed Counter-Current Chromatography (HSCCC) and Prep-HPLC Guided by DPPH-HPLC Experiments

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules22020229

DPPH-HPLC chromatograms of n -butanol extract of Lonicera japonica leaves. Experimental conditions: Shim-pack VP-ODS column (250 mm × 4.6 mm, i.d., 5 μm); Column temperature: 25 °C; Flow rate: 1.0 mL/min; Detection: 254 nm; Injection volume: 10 μL. HPLC conditions are as follows: eluent A (MeOH) and eluent B (0.3% acetic acid in water, v / v ), linear gradient combinations: at t = 0, 70% B; at t = 20 min, 40% B; at t = 22.5 min, 40% B. a.u.: arbitrary unit.
Figure Legend Snippet: DPPH-HPLC chromatograms of n -butanol extract of Lonicera japonica leaves. Experimental conditions: Shim-pack VP-ODS column (250 mm × 4.6 mm, i.d., 5 μm); Column temperature: 25 °C; Flow rate: 1.0 mL/min; Detection: 254 nm; Injection volume: 10 μL. HPLC conditions are as follows: eluent A (MeOH) and eluent B (0.3% acetic acid in water, v / v ), linear gradient combinations: at t = 0, 70% B; at t = 20 min, 40% B; at t = 22.5 min, 40% B. a.u.: arbitrary unit.

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry, Injection

HPLC chromatograms of prep-HPLC peak fractions. (( A ) chlorogenic acid, compound 1 in Figure 4 A; ( B ) lonicerin, compound 2 in Figure 4 A; ( C ) rutin, compound 3 in Figure 4 A; ( D ) 3,4- O -dicaffeoylquinic acid, compound 6 in Figure 4 D; ( E ) hyperoside, compound 7 in Figure 4 D; ( F ) 3,5- O -dicaffeoylquinic acid, compound 8 in Figure 4 E; ( G ) 4,5- O -dicaffeoylquinic acid, compound 9 in Figure 4 E). a.u.: arbitrary unit.
Figure Legend Snippet: HPLC chromatograms of prep-HPLC peak fractions. (( A ) chlorogenic acid, compound 1 in Figure 4 A; ( B ) lonicerin, compound 2 in Figure 4 A; ( C ) rutin, compound 3 in Figure 4 A; ( D ) 3,4- O -dicaffeoylquinic acid, compound 6 in Figure 4 D; ( E ) hyperoside, compound 7 in Figure 4 D; ( F ) 3,5- O -dicaffeoylquinic acid, compound 8 in Figure 4 E; ( G ) 4,5- O -dicaffeoylquinic acid, compound 9 in Figure 4 E). a.u.: arbitrary unit.

Techniques Used: High Performance Liquid Chromatography

HPLC chromatograms of n -butanol extract and HSCCC peak fractions from leaves of L. japonica . (( A ) F1 in Figure 3 ; ( B ) F2 in Figure 3 ; ( C ) F3 in Figure 3 ; ( D ) F4 in Figure 3 ; ( E ) F5 collected from the column after the separation). Experimental conditions: Shim-pack VP-ODS column (250 mm × 4.6 mm, i.d., 5 μm); Column temperature: 25 °C; Flow rate: 1.0 mL/min; Detection: 254 nm; Injection volume: 10 μL. HPLC conditions are as follows: Eluent A (MeOH) and eluent B (0.3% acetic acid in water, v/v ), linear gradient combinations: at t = 0, 70% B; at t = 20 min, 40% B; at t = 22.5 min, 40% B. a.u.: arbitrary unit.
Figure Legend Snippet: HPLC chromatograms of n -butanol extract and HSCCC peak fractions from leaves of L. japonica . (( A ) F1 in Figure 3 ; ( B ) F2 in Figure 3 ; ( C ) F3 in Figure 3 ; ( D ) F4 in Figure 3 ; ( E ) F5 collected from the column after the separation). Experimental conditions: Shim-pack VP-ODS column (250 mm × 4.6 mm, i.d., 5 μm); Column temperature: 25 °C; Flow rate: 1.0 mL/min; Detection: 254 nm; Injection volume: 10 μL. HPLC conditions are as follows: Eluent A (MeOH) and eluent B (0.3% acetic acid in water, v/v ), linear gradient combinations: at t = 0, 70% B; at t = 20 min, 40% B; at t = 22.5 min, 40% B. a.u.: arbitrary unit.

Techniques Used: High Performance Liquid Chromatography, High Speed Counter-current Chromatography, Flow Cytometry, Injection

9) Product Images from "Rutamarin: Efficient Liquid–Liquid Chromatographic Isolation from Ruta graveolens L. and Evaluation of Its In Vitro and In Silico MAO-B Inhibitory Activity"

Article Title: Rutamarin: Efficient Liquid–Liquid Chromatographic Isolation from Ruta graveolens L. and Evaluation of Its In Vitro and In Silico MAO-B Inhibitory Activity

Journal: Molecules

doi: 10.3390/molecules25112678

HPLC–DAD chromatograms of ( a ) crude dichloromethane extract of Ruta graveolens L. and ( b ) isolated rutamarin (λ=335 nm).
Figure Legend Snippet: HPLC–DAD chromatograms of ( a ) crude dichloromethane extract of Ruta graveolens L. and ( b ) isolated rutamarin (λ=335 nm).

Techniques Used: High Performance Liquid Chromatography, Isolation

10) Product Images from "Polyalcohols as Hydrogen-Bonding Donors in Choline Chloride-Based Deep Eutectic Solvents for Extraction of Xanthones from the Pericarp of Garcinia mangostana L."

Article Title: Polyalcohols as Hydrogen-Bonding Donors in Choline Chloride-Based Deep Eutectic Solvents for Extraction of Xanthones from the Pericarp of Garcinia mangostana L.

Journal: Molecules

doi: 10.3390/molecules24030636

Chromatogram of the DES-2P3 (above) and ethanolic extracts (below). Numbers indicate the xanthone components identified using LC-MS: ( 1 ) 1,7-dihydroxy-3-methoxy-2-(3-methylbut-2- enyl)xanthon, ( 2 ) γ-mangostin, ( 3 ) gartanin, ( 4 ) α-mangostin, ( 5 ) garcinone E, (6 ) garcimangosone B, ( 7 ) β-mangostin.
Figure Legend Snippet: Chromatogram of the DES-2P3 (above) and ethanolic extracts (below). Numbers indicate the xanthone components identified using LC-MS: ( 1 ) 1,7-dihydroxy-3-methoxy-2-(3-methylbut-2- enyl)xanthon, ( 2 ) γ-mangostin, ( 3 ) gartanin, ( 4 ) α-mangostin, ( 5 ) garcinone E, (6 ) garcimangosone B, ( 7 ) β-mangostin.

Techniques Used: Liquid Chromatography with Mass Spectroscopy

11) Product Images from "Effect of Lonicera caerulea var. emphyllocalyx Fruit on Biofilm Formed by Porphyromonas gingivalis"

Article Title: Effect of Lonicera caerulea var. emphyllocalyx Fruit on Biofilm Formed by Porphyromonas gingivalis

Journal: BioMed Research International

doi: 10.1155/2019/3547858

Effect of LCEE on the growth of three P. gingivalis isolates. Three P. gingivalis isolates (JCM12257, JCM8525, and JCM19600) were treated with or without LCEE (500 μ g/mL) for 24 h and their growth was quantified by measuring absorbance at 600 nm. Data represent the mean ± SD ( n = 3). LCEE: Lonicera caerulea var. emphyllocalyx extract.
Figure Legend Snippet: Effect of LCEE on the growth of three P. gingivalis isolates. Three P. gingivalis isolates (JCM12257, JCM8525, and JCM19600) were treated with or without LCEE (500 μ g/mL) for 24 h and their growth was quantified by measuring absorbance at 600 nm. Data represent the mean ± SD ( n = 3). LCEE: Lonicera caerulea var. emphyllocalyx extract.

Techniques Used:

Inhibitory effect of LCEE on biofilm formation by P. gingivalis evaluated by SEM assay. P. gingivalis JCM12257 was treated with or without LCEE (500 μ g/mL) for 72 h. Tracheal tube was involved in culture medium. Biofilm was analyzed by scanning electron microscopy (SEM). (a) Untreated bacteria. (b) LCEE-treated bacteria. LCEE: Lonicera caerulea var. emphyllocalyx extract.
Figure Legend Snippet: Inhibitory effect of LCEE on biofilm formation by P. gingivalis evaluated by SEM assay. P. gingivalis JCM12257 was treated with or without LCEE (500 μ g/mL) for 72 h. Tracheal tube was involved in culture medium. Biofilm was analyzed by scanning electron microscopy (SEM). (a) Untreated bacteria. (b) LCEE-treated bacteria. LCEE: Lonicera caerulea var. emphyllocalyx extract.

Techniques Used: Electron Microscopy

Chromatogram of LCEE. LCEE (5 μ g) was injected onto HPLC with the following conditions: system, Shimadzu LC–10A VP (Kyoto, Japan); column, TSK-GEL ODS-80 TS (4.6 × 250 mm, Tosoh, Tokyo); mobile phase, 0.5% AcOH/0.5% AcOH in CH 3 OH 85 : 15; flow rate, 1.0 mL/min; column temperature, 40°C; and detection, 520 nm. Peak at 9.0 min was identified as cyanidin 3-O-glucoside (C3G). LCEE: Lonicera caerulea var. emphyllocalyx extract; HPLC: high-performance liquid chromatography.
Figure Legend Snippet: Chromatogram of LCEE. LCEE (5 μ g) was injected onto HPLC with the following conditions: system, Shimadzu LC–10A VP (Kyoto, Japan); column, TSK-GEL ODS-80 TS (4.6 × 250 mm, Tosoh, Tokyo); mobile phase, 0.5% AcOH/0.5% AcOH in CH 3 OH 85 : 15; flow rate, 1.0 mL/min; column temperature, 40°C; and detection, 520 nm. Peak at 9.0 min was identified as cyanidin 3-O-glucoside (C3G). LCEE: Lonicera caerulea var. emphyllocalyx extract; HPLC: high-performance liquid chromatography.

Techniques Used: Injection, High Performance Liquid Chromatography, Flow Cytometry

12) Product Images from "Bergamot Reduces Plasma Lipids, Atherogenic Small Dense LDL, and Subclinical Atherosclerosis in Subjects with Moderate Hypercholesterolemia: A 6 Months Prospective Study"

Article Title: Bergamot Reduces Plasma Lipids, Atherogenic Small Dense LDL, and Subclinical Atherosclerosis in Subjects with Moderate Hypercholesterolemia: A 6 Months Prospective Study

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2015.00299

The components of Bergavit ® separated by high-performance liquid chromatography (HPLC-DAD Analysis 280 nm), as determined by Bionap. The chromatographic analyses are determined on a Shimadzu LC system by Bionap.
Figure Legend Snippet: The components of Bergavit ® separated by high-performance liquid chromatography (HPLC-DAD Analysis 280 nm), as determined by Bionap. The chromatographic analyses are determined on a Shimadzu LC system by Bionap.

Techniques Used: High Performance Liquid Chromatography

13) Product Images from "Successive Oral Immunizations Against Piscirickettsia Salmonis and Infectious Salmon Anemia Virus are Required to Maintain a Long-Term Protection in Farmed Salmonids"

Article Title: Successive Oral Immunizations Against Piscirickettsia Salmonis and Infectious Salmon Anemia Virus are Required to Maintain a Long-Term Protection in Farmed Salmonids

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2015.00244

Chromatography of purified IgM from S. salar . The IgM of Atlantic salmon previously separated by SEC was subjected to HPLC on dC18 column (Waters) and eluted under isocratic conditions. The chromatography was monitored at 280 nm.
Figure Legend Snippet: Chromatography of purified IgM from S. salar . The IgM of Atlantic salmon previously separated by SEC was subjected to HPLC on dC18 column (Waters) and eluted under isocratic conditions. The chromatography was monitored at 280 nm.

Techniques Used: Chromatography, Purification, Size-exclusion Chromatography, High Performance Liquid Chromatography

14) Product Images from "Function of a Glutamine Synthetase-Like Protein in Bacterial Aniline Oxidation via ?-Glutamylanilide"

Article Title: Function of a Glutamine Synthetase-Like Protein in Bacterial Aniline Oxidation via ?-Glutamylanilide

Journal: Journal of Bacteriology

doi: 10.1128/JB.00397-13

Conversion of aniline into catechol by a cell suspension of P. putida KT2440-Δ catA harboring pTB01 ( atdA1 to atdA5 ). Symbols: open circles, aniline; closed circles, catechol. The experiment was performed in triplicate, and averages ± standard deviations are shown.
Figure Legend Snippet: Conversion of aniline into catechol by a cell suspension of P. putida KT2440-Δ catA harboring pTB01 ( atdA1 to atdA5 ). Symbols: open circles, aniline; closed circles, catechol. The experiment was performed in triplicate, and averages ± standard deviations are shown.

Techniques Used:

Conversion of γ-GA into catechol by a cell suspension of P. putida KT2440-Δ catAΔggt harboring pTB01 ( atdA1 to atdA5 ). Symbols: open circles, γ-GA; closed circles, catechol; closed triangles, aniline. The experiment was performed in triplicate, and averages ± standard deviations are shown.
Figure Legend Snippet: Conversion of γ-GA into catechol by a cell suspension of P. putida KT2440-Δ catAΔggt harboring pTB01 ( atdA1 to atdA5 ). Symbols: open circles, γ-GA; closed circles, catechol; closed triangles, aniline. The experiment was performed in triplicate, and averages ± standard deviations are shown.

Techniques Used:

Conversion of aniline into γ-GA by AtdA1. Symbols: open circles, aniline; closed circles, γ-GA. The experiment was performed in triplicate, and averages ± standard deviations are shown.
Figure Legend Snippet: Conversion of aniline into γ-GA by AtdA1. Symbols: open circles, aniline; closed circles, γ-GA. The experiment was performed in triplicate, and averages ± standard deviations are shown.

Techniques Used:

Growth of Acinetobacter baylyi BD413 harboring pTB01 ( atdA1 to atdA5 ) (A) or pTB01-Δ A2 ( atdA1 and atdA3 to atdA5 ) (B) on aniline. Arrows indicate the addition of aniline. Symbols: open circles, aniline; closed circles, growth (OD 600 ); closed triangles, γ-GA.
Figure Legend Snippet: Growth of Acinetobacter baylyi BD413 harboring pTB01 ( atdA1 to atdA5 ) (A) or pTB01-Δ A2 ( atdA1 and atdA3 to atdA5 ) (B) on aniline. Arrows indicate the addition of aniline. Symbols: open circles, aniline; closed circles, growth (OD 600 ); closed triangles, γ-GA.

Techniques Used:

Conversion of aniline into catechol by cell suspensions of P. putida KT2440-Δ catAΔggt harboring pTB01 ( atdA1 to atdA5 ) (A) or pTB01-Δ A2 ( atdA1 and atdA3 to atdA5 ) (B). The experiments were performed in triplicate, and averages ± standard deviations are shown. Symbols: open circles, aniline; closed circles, catechol; closed triangles, γ-GA.
Figure Legend Snippet: Conversion of aniline into catechol by cell suspensions of P. putida KT2440-Δ catAΔggt harboring pTB01 ( atdA1 to atdA5 ) (A) or pTB01-Δ A2 ( atdA1 and atdA3 to atdA5 ) (B). The experiments were performed in triplicate, and averages ± standard deviations are shown. Symbols: open circles, aniline; closed circles, catechol; closed triangles, γ-GA.

Techniques Used:

15) Product Images from "Enhanced 2,3-Butanediol Production by Optimizing Fermentation Conditions and Engineering Klebsiella oxytoca M1 through Overexpression of Acetoin Reductase"

Article Title: Enhanced 2,3-Butanediol Production by Optimizing Fermentation Conditions and Engineering Klebsiella oxytoca M1 through Overexpression of Acetoin Reductase

Journal: PLoS ONE

doi: 10.1371/journal.pone.0138109

Effect of complex nitrogen source on 2,3-BDO fermentation. (a) dry cell weight and (b) 2,3-BDO production. K . oxytoca M1 was grown for 12 hours at 30°C and 200 rpm in an Erlenmeyer flask containing 60 g/L glucose. Growth medium used were the defined medium (□) and the defined medium supplemented with 5 g/L yeast extract and 10 g/L casamino acid (i.e. the modified medium) (■).
Figure Legend Snippet: Effect of complex nitrogen source on 2,3-BDO fermentation. (a) dry cell weight and (b) 2,3-BDO production. K . oxytoca M1 was grown for 12 hours at 30°C and 200 rpm in an Erlenmeyer flask containing 60 g/L glucose. Growth medium used were the defined medium (□) and the defined medium supplemented with 5 g/L yeast extract and 10 g/L casamino acid (i.e. the modified medium) (■).

Techniques Used: Modification

Time course of 2,3-butanediol production by K . oxytoca M1 at different agitation speeds. (a) 200 rpm, (b) 300 rpm, and (c) 400 rpm. Symbols indicate dry cell weight (■), residual glucose (□), 2,3-BDO (▲), and acetoin (▼).
Figure Legend Snippet: Time course of 2,3-butanediol production by K . oxytoca M1 at different agitation speeds. (a) 200 rpm, (b) 300 rpm, and (c) 400 rpm. Symbols indicate dry cell weight (■), residual glucose (□), 2,3-BDO (▲), and acetoin (▼).

Techniques Used:

Time course of 2,3-BDO fermentation by K . oxytoca M1 (pUC18CM- budC ) at different agitation speeds. (a) 300 rpm and (b) 400 rpm. Fed-batch fermentation was carried out with the medium containing 5 g/L yeast extract and 10 g/L casamino acid. Symbols indicate dry cell weight (■), residual glucose (□), 2,3-BDO (▲) and acetoin (▼).
Figure Legend Snippet: Time course of 2,3-BDO fermentation by K . oxytoca M1 (pUC18CM- budC ) at different agitation speeds. (a) 300 rpm and (b) 400 rpm. Fed-batch fermentation was carried out with the medium containing 5 g/L yeast extract and 10 g/L casamino acid. Symbols indicate dry cell weight (■), residual glucose (□), 2,3-BDO (▲) and acetoin (▼).

Techniques Used:

Acetoin reduction activity and 2,3-BDO oxidation activity of K . oxytoca M1 and K . oxytoca (pUC18CM- budC ). Activity was analyzed with the crude protein extract of each strain. Bars indicate acetoin reduction activity (■) and 2,3-BDO oxidation activity (□). One unit of specific activity was defined as 1 μmol NADH consumed or generated per minute per 1 mg crude protein.
Figure Legend Snippet: Acetoin reduction activity and 2,3-BDO oxidation activity of K . oxytoca M1 and K . oxytoca (pUC18CM- budC ). Activity was analyzed with the crude protein extract of each strain. Bars indicate acetoin reduction activity (■) and 2,3-BDO oxidation activity (□). One unit of specific activity was defined as 1 μmol NADH consumed or generated per minute per 1 mg crude protein.

Techniques Used: Activity Assay, Generated

16) Product Images from "Determination and Analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in False Smut Balls of Rice"

Article Title: Determination and Analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in False Smut Balls of Rice

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms130911275

Primary determination of the ustiloxin-like compounds from the water extract of rice false smut balls by LC-ESI-MS. ( A ) and ( B ) were the spectra of HPLC-UV and total ion chromatograms, respectively. ( C – G ) were the ESI-MS spectra of peaks 1, 2, 3, 4 and 5, respectively, appearing in ( A ) and ( B ).
Figure Legend Snippet: Primary determination of the ustiloxin-like compounds from the water extract of rice false smut balls by LC-ESI-MS. ( A ) and ( B ) were the spectra of HPLC-UV and total ion chromatograms, respectively. ( C – G ) were the ESI-MS spectra of peaks 1, 2, 3, 4 and 5, respectively, appearing in ( A ) and ( B ).

Techniques Used: Mass Spectrometry, High Performance Liquid Chromatography

17) Product Images from "Thiocarbamates from Moringa oleifera Seeds Bioactive against Virulent and Multidrug-Resistant Vibrio Species"

Article Title: Thiocarbamates from Moringa oleifera Seeds Bioactive against Virulent and Multidrug-Resistant Vibrio Species

Journal: BioMed Research International

doi: 10.1155/2017/7963747

HPLC chromatogram analysis of an active fraction of MOS-ESA and isolation of MOS-ES-1, MOS-ES-2, and MOS-ES-3.
Figure Legend Snippet: HPLC chromatogram analysis of an active fraction of MOS-ESA and isolation of MOS-ES-1, MOS-ES-2, and MOS-ES-3.

Techniques Used: High Performance Liquid Chromatography, Isolation

18) Product Images from "Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition"

Article Title: Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition

Journal: Toxins

doi: 10.3390/toxins10050177

Distribution of protein classes in venom from male (BmooM) and female (BmooF) B. moojeni snakes determined by shotgun-proteomics. ( A ) Relative distribution of protein classes in BmooM and BmooF venom. AMPs: antimicrobial peptides, BPPs: bradykinin-potentiating peptides, Cell Prots: cellular proteins, C-Lectins: C-type lectins, CRISPs: cysteine-rich secretory proteins, HYALs: hyaluronidases, LAAOs: L-amino acid oxidases, NCTDs: nucleotidases, PEPs: peptidases, PLs: phospholipases, PL INBs: phospholipase inhibitors, PPDTs: phosphodiesterases, SVMPs: snake venom metalloproteinases, SVSPs: snake venom serine proteases, VEGFs: vascular endothelial growth factors; ( B ) Venn diagram with the proteins identified by mass spectrometry.
Figure Legend Snippet: Distribution of protein classes in venom from male (BmooM) and female (BmooF) B. moojeni snakes determined by shotgun-proteomics. ( A ) Relative distribution of protein classes in BmooM and BmooF venom. AMPs: antimicrobial peptides, BPPs: bradykinin-potentiating peptides, Cell Prots: cellular proteins, C-Lectins: C-type lectins, CRISPs: cysteine-rich secretory proteins, HYALs: hyaluronidases, LAAOs: L-amino acid oxidases, NCTDs: nucleotidases, PEPs: peptidases, PLs: phospholipases, PL INBs: phospholipase inhibitors, PPDTs: phosphodiesterases, SVMPs: snake venom metalloproteinases, SVSPs: snake venom serine proteases, VEGFs: vascular endothelial growth factors; ( B ) Venn diagram with the proteins identified by mass spectrometry.

Techniques Used: Mass Spectrometry

Functional characterization of B. moojeni venom stratified by snake gender. BmooF = female snake; BmooM = male snake. ( A ) Amount of venom released during manual extractions; ( B ) L-amino acid oxidase specific activity determined using o -phenylenediamine as substrate; ( C ) Hyaluronidase specific activity determined by hydrolysis of hyaluronan, measured through a turbidimetric assay. TRU: turbidity-reducing units; ( D ) Metalloproteinase activity determined using the azocasein assay. Results are expressed as relative inhibition of the activity after venom incubation with ethylenediamine tetraacetic acid (EDTA); ( E ) Phospholipase activity determined using egg-yolk agar plate medium. The size of the halos formed indicate the rate of phospholipase activity; ( F ) Serine protease specific activity determined using Nα- p -tosyl-L-arginine methyl ester (TAME) as substrate. The results are expressed as mean ± SD. Statistics (Student’s t test): ( A ) * p
Figure Legend Snippet: Functional characterization of B. moojeni venom stratified by snake gender. BmooF = female snake; BmooM = male snake. ( A ) Amount of venom released during manual extractions; ( B ) L-amino acid oxidase specific activity determined using o -phenylenediamine as substrate; ( C ) Hyaluronidase specific activity determined by hydrolysis of hyaluronan, measured through a turbidimetric assay. TRU: turbidity-reducing units; ( D ) Metalloproteinase activity determined using the azocasein assay. Results are expressed as relative inhibition of the activity after venom incubation with ethylenediamine tetraacetic acid (EDTA); ( E ) Phospholipase activity determined using egg-yolk agar plate medium. The size of the halos formed indicate the rate of phospholipase activity; ( F ) Serine protease specific activity determined using Nα- p -tosyl-L-arginine methyl ester (TAME) as substrate. The results are expressed as mean ± SD. Statistics (Student’s t test): ( A ) * p

Techniques Used: Functional Assay, Activity Assay, Turbidimetric Assay, Azocasein Assay, Inhibition, Incubation

Analysis of venom composition in male (BmooM) and female (BmooF) B. moojeni snakes using HPLC coupled to MALDI-TOF mass spectrometry. ( A ) Comparative chromatographic profile of BmooM and BmooF venom obtained after reversed-phase HPLC analysis using a linear concentration gradient of 70% acetonitrile (solvent A) and 0.1% trifluoroacetic acid (solvent B), at a flow rate of 1 mL/min. The numbers represent the elution time (in minutes) of five fractions whose peak intensity and/or retention time differed considerably between the snake genders; ( B ) Venn diagram of the number of ions obtained by MALDI-TOF mass spectrometry analysis of the five fractions selected in ( A ).
Figure Legend Snippet: Analysis of venom composition in male (BmooM) and female (BmooF) B. moojeni snakes using HPLC coupled to MALDI-TOF mass spectrometry. ( A ) Comparative chromatographic profile of BmooM and BmooF venom obtained after reversed-phase HPLC analysis using a linear concentration gradient of 70% acetonitrile (solvent A) and 0.1% trifluoroacetic acid (solvent B), at a flow rate of 1 mL/min. The numbers represent the elution time (in minutes) of five fractions whose peak intensity and/or retention time differed considerably between the snake genders; ( B ) Venn diagram of the number of ions obtained by MALDI-TOF mass spectrometry analysis of the five fractions selected in ( A ).

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Concentration Assay, Flow Cytometry

19) Product Images from "Dry Powder and Budesonide Inhalation Suspension Deposition Rates in Asthmatic Airway-Obstruction Regions"

Article Title: Dry Powder and Budesonide Inhalation Suspension Deposition Rates in Asthmatic Airway-Obstruction Regions

Journal: Journal of Drug Delivery

doi: 10.1155/2019/3921426

(a) Mass median aerodynamic diameters (MMAD) of the particle size distribution. Turbuhaler, 200 µ g; MMAD, 2.20 µ m, (b) Mass median aerodynamic diameters (MMAD) of the particle size distribution. Symbicort Turbuhaler, 60 DPI; MMAD, 2.60 µ m, (c) Mass median aerodynamic diameters (MMAD) of the particle size distribution. NE-U22; budesonide suspension, 250 µ g/mL; MMAD, 4.52 µ m, and (d) Mass median aerodynamic diameters (MMAD) of the particle size distribution. NE-C28; budesonide suspension, 250 µ g/mL; MMAD, 3.84 µ m.
Figure Legend Snippet: (a) Mass median aerodynamic diameters (MMAD) of the particle size distribution. Turbuhaler, 200 µ g; MMAD, 2.20 µ m, (b) Mass median aerodynamic diameters (MMAD) of the particle size distribution. Symbicort Turbuhaler, 60 DPI; MMAD, 2.60 µ m, (c) Mass median aerodynamic diameters (MMAD) of the particle size distribution. NE-U22; budesonide suspension, 250 µ g/mL; MMAD, 4.52 µ m, and (d) Mass median aerodynamic diameters (MMAD) of the particle size distribution. NE-C28; budesonide suspension, 250 µ g/mL; MMAD, 3.84 µ m.

Techniques Used:

20) Product Images from "Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition"

Article Title: Proteopeptidomic, Functional and Immunoreactivity Characterization of Bothrops moojeni Snake Venom: Influence of Snake Gender on Venom Composition

Journal: Toxins

doi: 10.3390/toxins10050177

Distribution of protein classes in venom from male (BmooM) and female (BmooF) B. moojeni snakes determined by shotgun-proteomics. ( A ) Relative distribution of protein classes in BmooM and BmooF venom. AMPs: antimicrobial peptides, BPPs: bradykinin-potentiating peptides, Cell Prots: cellular proteins, C-Lectins: C-type lectins, CRISPs: cysteine-rich secretory proteins, HYALs: hyaluronidases, LAAOs: L-amino acid oxidases, NCTDs: nucleotidases, PEPs: peptidases, PLs: phospholipases, PL INBs: phospholipase inhibitors, PPDTs: phosphodiesterases, SVMPs: snake venom metalloproteinases, SVSPs: snake venom serine proteases, VEGFs: vascular endothelial growth factors; ( B ) Venn diagram with the proteins identified by mass spectrometry.
Figure Legend Snippet: Distribution of protein classes in venom from male (BmooM) and female (BmooF) B. moojeni snakes determined by shotgun-proteomics. ( A ) Relative distribution of protein classes in BmooM and BmooF venom. AMPs: antimicrobial peptides, BPPs: bradykinin-potentiating peptides, Cell Prots: cellular proteins, C-Lectins: C-type lectins, CRISPs: cysteine-rich secretory proteins, HYALs: hyaluronidases, LAAOs: L-amino acid oxidases, NCTDs: nucleotidases, PEPs: peptidases, PLs: phospholipases, PL INBs: phospholipase inhibitors, PPDTs: phosphodiesterases, SVMPs: snake venom metalloproteinases, SVSPs: snake venom serine proteases, VEGFs: vascular endothelial growth factors; ( B ) Venn diagram with the proteins identified by mass spectrometry.

Techniques Used: Mass Spectrometry

Functional characterization of B. moojeni venom stratified by snake gender. BmooF = female snake; BmooM = male snake. ( A ) Amount of venom released during manual extractions; ( B ) L-amino acid oxidase specific activity determined using o -phenylenediamine as substrate; ( C ) Hyaluronidase specific activity determined by hydrolysis of hyaluronan, measured through a turbidimetric assay. TRU: turbidity-reducing units; ( D ) Metalloproteinase activity determined using the azocasein assay. Results are expressed as relative inhibition of the activity after venom incubation with ethylenediamine tetraacetic acid (EDTA); ( E ) Phospholipase activity determined using egg-yolk agar plate medium. The size of the halos formed indicate the rate of phospholipase activity; ( F ) Serine protease specific activity determined using Nα- p -tosyl-L-arginine methyl ester (TAME) as substrate. The results are expressed as mean ± SD. Statistics (Student’s t test): ( A ) * p
Figure Legend Snippet: Functional characterization of B. moojeni venom stratified by snake gender. BmooF = female snake; BmooM = male snake. ( A ) Amount of venom released during manual extractions; ( B ) L-amino acid oxidase specific activity determined using o -phenylenediamine as substrate; ( C ) Hyaluronidase specific activity determined by hydrolysis of hyaluronan, measured through a turbidimetric assay. TRU: turbidity-reducing units; ( D ) Metalloproteinase activity determined using the azocasein assay. Results are expressed as relative inhibition of the activity after venom incubation with ethylenediamine tetraacetic acid (EDTA); ( E ) Phospholipase activity determined using egg-yolk agar plate medium. The size of the halos formed indicate the rate of phospholipase activity; ( F ) Serine protease specific activity determined using Nα- p -tosyl-L-arginine methyl ester (TAME) as substrate. The results are expressed as mean ± SD. Statistics (Student’s t test): ( A ) * p

Techniques Used: Functional Assay, Activity Assay, Turbidimetric Assay, Azocasein Assay, Inhibition, Incubation

Analysis of venom composition in male (BmooM) and female (BmooF) B. moojeni snakes using HPLC coupled to MALDI-TOF mass spectrometry. ( A ) Comparative chromatographic profile of BmooM and BmooF venom obtained after reversed-phase HPLC analysis using a linear concentration gradient of 70% acetonitrile (solvent A) and 0.1% trifluoroacetic acid (solvent B), at a flow rate of 1 mL/min. The numbers represent the elution time (in minutes) of five fractions whose peak intensity and/or retention time differed considerably between the snake genders; ( B ) Venn diagram of the number of ions obtained by MALDI-TOF mass spectrometry analysis of the five fractions selected in ( A ).
Figure Legend Snippet: Analysis of venom composition in male (BmooM) and female (BmooF) B. moojeni snakes using HPLC coupled to MALDI-TOF mass spectrometry. ( A ) Comparative chromatographic profile of BmooM and BmooF venom obtained after reversed-phase HPLC analysis using a linear concentration gradient of 70% acetonitrile (solvent A) and 0.1% trifluoroacetic acid (solvent B), at a flow rate of 1 mL/min. The numbers represent the elution time (in minutes) of five fractions whose peak intensity and/or retention time differed considerably between the snake genders; ( B ) Venn diagram of the number of ions obtained by MALDI-TOF mass spectrometry analysis of the five fractions selected in ( A ).

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Concentration Assay, Flow Cytometry

21) Product Images from "SELDI-TOF-MS ProteinChip array profiling of T-cell clones propagated in long-term culture identifies human profilin-1 as a potential bio-marker of immunosenescence"

Article Title: SELDI-TOF-MS ProteinChip array profiling of T-cell clones propagated in long-term culture identifies human profilin-1 as a potential bio-marker of immunosenescence

Journal: Proteome Science

doi: 10.1186/1477-5956-5-7

Comparative m/z determination of the 14.9 kDa differentially expressed protein/peptide peak . Lysates from several clones were pooled and enriched under the same reversed-phase conditions that were used in the H50 ProteinChip SELDI analysis. Reversed-phase high-performance liquid chromatography (HPLC) was performed in order to compare mass obtained from SELDI analysis to more accurate MALDI-TOF MS. HPLC-purified samples were run in parallel on SELDI-TOF (on a normal-phase ProteinChip) and MALDI-TOF mass spectrometers. One peak of 14.9 kDa identified as differentially expressed in T-cell senescence is demonstrated by MALDI-TOF (a) and SELDI-TOF (b) mass spectrometric analysis.
Figure Legend Snippet: Comparative m/z determination of the 14.9 kDa differentially expressed protein/peptide peak . Lysates from several clones were pooled and enriched under the same reversed-phase conditions that were used in the H50 ProteinChip SELDI analysis. Reversed-phase high-performance liquid chromatography (HPLC) was performed in order to compare mass obtained from SELDI analysis to more accurate MALDI-TOF MS. HPLC-purified samples were run in parallel on SELDI-TOF (on a normal-phase ProteinChip) and MALDI-TOF mass spectrometers. One peak of 14.9 kDa identified as differentially expressed in T-cell senescence is demonstrated by MALDI-TOF (a) and SELDI-TOF (b) mass spectrometric analysis.

Techniques Used: Clone Assay, High Performance Liquid Chromatography, Mass Spectrometry, Purification

22) Product Images from "Quantitative Analysis and Comparison of Four Major Flavonol Glycosides in the Leaves of Toona sinensis (A. Juss.) Roemer (Chinese Toon) from Various Origins by High-Performance Liquid Chromatography-Diode Array Detector and Hierarchical Clustering Analysis"

Article Title: Quantitative Analysis and Comparison of Four Major Flavonol Glycosides in the Leaves of Toona sinensis (A. Juss.) Roemer (Chinese Toon) from Various Origins by High-Performance Liquid Chromatography-Diode Array Detector and Hierarchical Clustering Analysis

Journal: Pharmacognosy Magazine

doi: 10.4103/0973-1296.182160

Typical high-performance liquid chromatography chromatograms of sample solutions from 11 origins (a-k) and mixed standards (l)
Figure Legend Snippet: Typical high-performance liquid chromatography chromatograms of sample solutions from 11 origins (a-k) and mixed standards (l)

Techniques Used: High Performance Liquid Chromatography

23) Product Images from "Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine"

Article Title: Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine

Journal: Journal of Pharmaceutical Analysis

doi: 10.1016/j.jpha.2020.05.011

Representative chromatograms in UPLC-MSMS analysis: (A) 20 ng/mL equimolar mixture of the 4 deoxyribonucleotides; (B) 20 ng/mL CT DNA after nuclease P1 digestion; (C) 100 μg/mL CT DNA spiked with 100 μg/mL ribonucleotide equimolar mixture, after nuclease P1 digestion (200-fold diluted before injection); (D) DNA isolated from mouse tumor, after nuclease P1 digestion (200-fold diluted before injection); (E) 20 ng/mL CT DNA after DNase I digestion.
Figure Legend Snippet: Representative chromatograms in UPLC-MSMS analysis: (A) 20 ng/mL equimolar mixture of the 4 deoxyribonucleotides; (B) 20 ng/mL CT DNA after nuclease P1 digestion; (C) 100 μg/mL CT DNA spiked with 100 μg/mL ribonucleotide equimolar mixture, after nuclease P1 digestion (200-fold diluted before injection); (D) DNA isolated from mouse tumor, after nuclease P1 digestion (200-fold diluted before injection); (E) 20 ng/mL CT DNA after DNase I digestion.

Techniques Used: Injection, Isolation

24) Product Images from "Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity"

Article Title: Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity

Journal: eLife

doi: 10.7554/eLife.56717

Functional analysis of AACS1 and AECH1 in S. pennellii and S. quitoense via CRISPR-Cas9 system and VIGS, respectively. The design of gRNAs targeting wild tomato S. pennellii LA0716 Sp-AACS1 ( A ), Sopen07g023250 ( B ), and Sp-AECH1 ( C ) is highlighted with red lines and text. The transgenic T0 generation carrying chimeric or biallelic gene edits are shown beneath the gene model. DNA sequence of the gene edits was obtained through Sanger sequencing of cloned plant DNA fragments. The gene edits are highlighted with dotted rectangular boxes. ( D ) ESI + mode, LC/MS extracted ion chromatograms shown for C10 ( m/z : 155.14) and C12 ( m/z : 183.17) fatty acid ions corresponding to medium chain trichome acylsugars extracted from the CRISPR mutants sopen07g023220 and the S. pennellii LA0716 parent. ( E ) Experimental design of VIGS in S. quitoense . A control group silencing the PDS genes was performed in parallel with the experimental groups. The onset of the albino phenotype of the control group was used as a visual marker to determine the harvest time and leaf selection in the experimental groups. The fourth true leaves were harvested and cut in half for gene expression analysis and acylsugar quantification, respectively. ( F ) ESI - mode, LC/MS extracted ion chromatograms of six major acylsugars of S. quitoense for the experimental group. The three LC/MS chromatograms show representative acylsugar profiles of the empty vector control plants and the VIGS plants targeting Sq-AACS1 and Sq-AECH1 .
Figure Legend Snippet: Functional analysis of AACS1 and AECH1 in S. pennellii and S. quitoense via CRISPR-Cas9 system and VIGS, respectively. The design of gRNAs targeting wild tomato S. pennellii LA0716 Sp-AACS1 ( A ), Sopen07g023250 ( B ), and Sp-AECH1 ( C ) is highlighted with red lines and text. The transgenic T0 generation carrying chimeric or biallelic gene edits are shown beneath the gene model. DNA sequence of the gene edits was obtained through Sanger sequencing of cloned plant DNA fragments. The gene edits are highlighted with dotted rectangular boxes. ( D ) ESI + mode, LC/MS extracted ion chromatograms shown for C10 ( m/z : 155.14) and C12 ( m/z : 183.17) fatty acid ions corresponding to medium chain trichome acylsugars extracted from the CRISPR mutants sopen07g023220 and the S. pennellii LA0716 parent. ( E ) Experimental design of VIGS in S. quitoense . A control group silencing the PDS genes was performed in parallel with the experimental groups. The onset of the albino phenotype of the control group was used as a visual marker to determine the harvest time and leaf selection in the experimental groups. The fourth true leaves were harvested and cut in half for gene expression analysis and acylsugar quantification, respectively. ( F ) ESI - mode, LC/MS extracted ion chromatograms of six major acylsugars of S. quitoense for the experimental group. The three LC/MS chromatograms show representative acylsugar profiles of the empty vector control plants and the VIGS plants targeting Sq-AACS1 and Sq-AECH1 .

Techniques Used: Functional Assay, CRISPR, Transgenic Assay, Sequencing, Clone Assay, Liquid Chromatography with Mass Spectroscopy, Marker, Selection, Expressing, Plasmid Preparation

25) Product Images from "Exogenous cysteine and cystine promote cell proliferation in CaCo‐2 cells"

Article Title: Exogenous cysteine and cystine promote cell proliferation in CaCo‐2 cells

Journal: Cell Proliferation

doi: 10.1046/j.1365-2184.2002.00229.x

High performance liquid chromatography separation of cystine, cysteine, GSH and GSSG in extracts of CaCo‐2 cells. (a) Separation of standard mixtures. (b) Separation of cystine, cysteine, GSH and GSSG in cell extracts. The numbers 1–4 represent the cystine, cysteine, GSH, and GSSG peaks with retention times of 12, 19, 25 and 27 min, respectively.
Figure Legend Snippet: High performance liquid chromatography separation of cystine, cysteine, GSH and GSSG in extracts of CaCo‐2 cells. (a) Separation of standard mixtures. (b) Separation of cystine, cysteine, GSH and GSSG in cell extracts. The numbers 1–4 represent the cystine, cysteine, GSH, and GSSG peaks with retention times of 12, 19, 25 and 27 min, respectively.

Techniques Used: High Performance Liquid Chromatography

26) Product Images from "Scanning Electron Microscopy and Liquid Chromatography for Physical and Chemical Inspection of Industrial Pharmaceutical Traditional Chinese Herbal Medicine"

Article Title: Scanning Electron Microscopy and Liquid Chromatography for Physical and Chemical Inspection of Industrial Pharmaceutical Traditional Chinese Herbal Medicine

Journal: ACS Omega

doi: 10.1021/acsomega.0c00809

HPLC chromatograms of the three components, baicalin, berberine, and rhein. The three compounds were detected under individual wavelengths, which were (A) 276 nm for baicalin, (B) 238 nm for berberine, and (C) 228 nm for rhein. 1: baicalin, 2: berberine, and 3: rhein.
Figure Legend Snippet: HPLC chromatograms of the three components, baicalin, berberine, and rhein. The three compounds were detected under individual wavelengths, which were (A) 276 nm for baicalin, (B) 238 nm for berberine, and (C) 228 nm for rhein. 1: baicalin, 2: berberine, and 3: rhein.

Techniques Used: High Performance Liquid Chromatography

HPLC chromatograms of brand E extracts after 100-fold dilution. (A) Baicalin (8.27 μg/mL), retention time: 21.4 min, detection wavelength: 276 nm; (B) berberine (0.81 μg/mL), retention time: 24.4 min, detection wavelength: 238 nm; and (C) rhein (0.60 μg/mL), retention time: 32.7 min, detection wavelength: 228 nm. 1: baicalin, 2: berberine, and 3: rhein.
Figure Legend Snippet: HPLC chromatograms of brand E extracts after 100-fold dilution. (A) Baicalin (8.27 μg/mL), retention time: 21.4 min, detection wavelength: 276 nm; (B) berberine (0.81 μg/mL), retention time: 24.4 min, detection wavelength: 238 nm; and (C) rhein (0.60 μg/mL), retention time: 32.7 min, detection wavelength: 228 nm. 1: baicalin, 2: berberine, and 3: rhein.

Techniques Used: High Performance Liquid Chromatography

27) Product Images from "Intestinal Anti-Inflammatory Activity of Baccharis dracunculifolia in the Trinitrobenzenesulphonic Acid Model of Rat Colitis"

Article Title: Intestinal Anti-Inflammatory Activity of Baccharis dracunculifolia in the Trinitrobenzenesulphonic Acid Model of Rat Colitis

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1093/ecam/nep081

Main effects of the B. dracunculifolia on the inflammatory intestinal process induced by TNBS in rats. Black lines indicate inhibition and black arrows indicate counteraction of depletion induced by the inflammatory process.
Figure Legend Snippet: Main effects of the B. dracunculifolia on the inflammatory intestinal process induced by TNBS in rats. Black lines indicate inhibition and black arrows indicate counteraction of depletion induced by the inflammatory process.

Techniques Used: Inhibition

Structures of chemical constituents in the ethyl acetate extract of B. dracunculifolia . ( 1 ) caffeic acid; ( 2 ) p -coumaric acid; ( 3 ) aromadendrin-4- O -methyl ether; ( 4 ) 3-prenyl- p -coumaric acid (drupanin); ( 5 ) 3,5-diprenyl p -coumaric acid (artepillin C); ( 6 ) baccharin.
Figure Legend Snippet: Structures of chemical constituents in the ethyl acetate extract of B. dracunculifolia . ( 1 ) caffeic acid; ( 2 ) p -coumaric acid; ( 3 ) aromadendrin-4- O -methyl ether; ( 4 ) 3-prenyl- p -coumaric acid (drupanin); ( 5 ) 3,5-diprenyl p -coumaric acid (artepillin C); ( 6 ) baccharin.

Techniques Used:

HPLC profile of B. dracunculifolia : ( 1 ) caffeic acid; ( 2 ) p -coumaric acid; ( 3 ) aromadendrin-4- O -methyl ether; ( 4 ) 3-prenyl- p -coumaric acid (drupanin); ( 5 ) 3,5-diprenyl p -coumaric acid (artepillin C); ( 6 ) baccharin.
Figure Legend Snippet: HPLC profile of B. dracunculifolia : ( 1 ) caffeic acid; ( 2 ) p -coumaric acid; ( 3 ) aromadendrin-4- O -methyl ether; ( 4 ) 3-prenyl- p -coumaric acid (drupanin); ( 5 ) 3,5-diprenyl p -coumaric acid (artepillin C); ( 6 ) baccharin.

Techniques Used: High Performance Liquid Chromatography

28) Product Images from "A Simplified, Specific HPLC Method of Assaying Thiamine and Riboflavin in Mushrooms"

Article Title: A Simplified, Specific HPLC Method of Assaying Thiamine and Riboflavin in Mushrooms

Journal: International Journal of Food Science

doi: 10.1155/2019/8716986

HPLC chromatograms obtained in peak identification and spectral analysis of riboflavin (B2) using photodiode array detector (PDA, 2996-Waters): (a) absorbance spectra of B2 from 190nm to 800nm, (b) reference standard, (c) purity plot of the B2 in standard solution, (d) reference standards solution, (e) purity plot of the B2 in test sample spiked with reference standards, and (f) test sample spiked with reference standards.
Figure Legend Snippet: HPLC chromatograms obtained in peak identification and spectral analysis of riboflavin (B2) using photodiode array detector (PDA, 2996-Waters): (a) absorbance spectra of B2 from 190nm to 800nm, (b) reference standard, (c) purity plot of the B2 in standard solution, (d) reference standards solution, (e) purity plot of the B2 in test sample spiked with reference standards, and (f) test sample spiked with reference standards.

Techniques Used: High Performance Liquid Chromatography

HPLC chromatograms obtained in peak identification and spectral analysis of thiamine (B1) using photodiode array detector (PDA, 2996-Waters): (a) absorbance spectra of B2 from 190nm to 800nm, (b) reference standard, (c) purity plot of the B1 in standard solution, (d) reference standards solution, (e) purity plot of the B1 in test sample spiked with reference standards, and (f) test sample spiked with reference standards.
Figure Legend Snippet: HPLC chromatograms obtained in peak identification and spectral analysis of thiamine (B1) using photodiode array detector (PDA, 2996-Waters): (a) absorbance spectra of B2 from 190nm to 800nm, (b) reference standard, (c) purity plot of the B1 in standard solution, (d) reference standards solution, (e) purity plot of the B1 in test sample spiked with reference standards, and (f) test sample spiked with reference standards.

Techniques Used: High Performance Liquid Chromatography

HPLC chromatograms obtained from Shimadzu prominence HPLC system with a UV-Vis detector (SPD-20AC) in method validation studies: (a) isocratic elution parameters, (b) diluting solution, (c) reference standards solution, (d) test sample spiked with reference standards, (e) LOD solution, and (f) test sample (Shitaki mushrooms).
Figure Legend Snippet: HPLC chromatograms obtained from Shimadzu prominence HPLC system with a UV-Vis detector (SPD-20AC) in method validation studies: (a) isocratic elution parameters, (b) diluting solution, (c) reference standards solution, (d) test sample spiked with reference standards, (e) LOD solution, and (f) test sample (Shitaki mushrooms).

Techniques Used: High Performance Liquid Chromatography

29) Product Images from "Biosynthesis of phlorisovalerophenone and 4-hydroxy-6-isobutyl-2-pyrone in Escherichia coli from glucose"

Article Title: Biosynthesis of phlorisovalerophenone and 4-hydroxy-6-isobutyl-2-pyrone in Escherichia coli from glucose

Journal: Microbial Cell Factories

doi: 10.1186/s12934-016-0549-9

Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone (HIBP) and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g HPLC analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3
Figure Legend Snippet: Engineered synthesis of 4-hydroxy-6-isovaleryl-2-pyrone (HIBP) and phlorisovalerophenone (PIVP) by E. coli strains. a , b Strain APG-0 harboring empty vectors as negative control. c , d Strain APG-1 harboring plasmids pET-A and pCDF-EV1. e , f Strain APG-2 harboring plasmids pET-A and pCDF-EV2. g , h Strain APG-3 harboring plasmids pET-A and pCDF-EV3. a , c , e , g HPLC analysis of metabolites extracted from cell pellets. b , d , f , h HPLC analysis of metabolites extracted from the fermentation broth. i The titers of HIBP produced by the strains APG-1, APG-2 and APG-3. j The titers of PIVP produced by the strains APG-1, APG-2 and APG-3

Techniques Used: Negative Control, Positron Emission Tomography, High Performance Liquid Chromatography, Produced

30) Product Images from "Quantification of imatinib in human serum: validation of a high-performance liquid chromatography-mass spectrometry method for therapeutic drug monitoring and pharmacokinetic assays"

Article Title: Quantification of imatinib in human serum: validation of a high-performance liquid chromatography-mass spectrometry method for therapeutic drug monitoring and pharmacokinetic assays

Journal: Drug Design, Development and Therapy

doi: 10.2147/DDDT.S42902

Chemical structures of imatinib ( A ) and tamsulosin ( B ) used as internal standard.
Figure Legend Snippet: Chemical structures of imatinib ( A ) and tamsulosin ( B ) used as internal standard.

Techniques Used:

31) Product Images from "From the Field to the Pot: Phytochemical and Functional Analyses of Calendula officinalis L. Flower for Incorporation in an Organic Yogurt"

Article Title: From the Field to the Pot: Phytochemical and Functional Analyses of Calendula officinalis L. Flower for Incorporation in an Organic Yogurt

Journal: Antioxidants

doi: 10.3390/antiox8110559

Principal component analysis based on physicochemical properties and antioxidant activity of marigold flower extracts ( Calendula officinalis L.). Extract 1 = 100% H 2 O:0% EtOH; extract 2 = 75% H 2 O:25% EtOH; extract 3 = 50% H 2 O:50% EtOH; extract 4 = 25% H 2 O:75% EtOH; and extract 5 = 0% H 2 O:100% EtOH. TPC = total phenolic content; FCRC = Folin–Ciocalteau reducing capacity.
Figure Legend Snippet: Principal component analysis based on physicochemical properties and antioxidant activity of marigold flower extracts ( Calendula officinalis L.). Extract 1 = 100% H 2 O:0% EtOH; extract 2 = 75% H 2 O:25% EtOH; extract 3 = 50% H 2 O:50% EtOH; extract 4 = 25% H 2 O:75% EtOH; and extract 5 = 0% H 2 O:100% EtOH. TPC = total phenolic content; FCRC = Folin–Ciocalteau reducing capacity.

Techniques Used: Antioxidant Activity Assay

In vitro antihemolytic effect of lyophilized marigold extract ( C. officinalis ). ( A ) Effects on hemolysis of human erythrocytes at different concentrations of NaCl; ( B ) effects of different extract concentrations on hemolysis of human erythrocytes at 0.3% (w/v) NaCl. Different letters (a–d) represent statistically significant differences, p
Figure Legend Snippet: In vitro antihemolytic effect of lyophilized marigold extract ( C. officinalis ). ( A ) Effects on hemolysis of human erythrocytes at different concentrations of NaCl; ( B ) effects of different extract concentrations on hemolysis of human erythrocytes at 0.3% (w/v) NaCl. Different letters (a–d) represent statistically significant differences, p

Techniques Used: In Vitro

Cell viability of A549, HCT8, and IMR90 cells in relation to different concentrations of lyophilized marigold extract ( C. officinalis ). ( A ) IC 50 : The concentration of the agent that inhibits growth by 50% is the concentration at which (T/C) × 100 = 50, where T = number of cells, at time t of treatment; C = control cells at time t of treatment. ( B ) GI 50 : The concentration of the agent that inhibits growth by 50%, relative to untreated cells, is the concentration at which ([T−T0]/[C−[]T0]) × 100 = 50, where T and C are the number of treated and control cells, respectively, at time t of treatment and T > T0; T0 is the number of cells at time zero. ( C ) LC 50 : The concentration of the agent that results in a net loss of 50% cells, relative to the number at the start of treatment, is the concentration at which ([T−T0]/T0) × 100 = −50; T
Figure Legend Snippet: Cell viability of A549, HCT8, and IMR90 cells in relation to different concentrations of lyophilized marigold extract ( C. officinalis ). ( A ) IC 50 : The concentration of the agent that inhibits growth by 50% is the concentration at which (T/C) × 100 = 50, where T = number of cells, at time t of treatment; C = control cells at time t of treatment. ( B ) GI 50 : The concentration of the agent that inhibits growth by 50%, relative to untreated cells, is the concentration at which ([T−T0]/[C−[]T0]) × 100 = 50, where T and C are the number of treated and control cells, respectively, at time t of treatment and T > T0; T0 is the number of cells at time zero. ( C ) LC 50 : The concentration of the agent that results in a net loss of 50% cells, relative to the number at the start of treatment, is the concentration at which ([T−T0]/T0) × 100 = −50; T

Techniques Used: Concentration Assay

In vitro inhibitory effects of different concentrations of lyophilized marigold extract ( C. officinalis ) on the activities of α-amylase ( A ) and α-glucosidase ( B ) enzymes. Different letters (a–e) represent statistically significant differences ( p
Figure Legend Snippet: In vitro inhibitory effects of different concentrations of lyophilized marigold extract ( C. officinalis ) on the activities of α-amylase ( A ) and α-glucosidase ( B ) enzymes. Different letters (a–e) represent statistically significant differences ( p

Techniques Used: In Vitro

Inhibition of lipid oxidation of Wistar rat brain treated with lyophilized marigold extract ( C. officinalis ) in comparison to quercetin. Probability value was obtained using unpaired Student t text.
Figure Legend Snippet: Inhibition of lipid oxidation of Wistar rat brain treated with lyophilized marigold extract ( C. officinalis ) in comparison to quercetin. Probability value was obtained using unpaired Student t text.

Techniques Used: Inhibition

Results of intracellular ROS measurement in A549 ( A ) and IMR90 ( B ) cells by spectrofluoremetry. Treatment = lyophilized marigold extract ( C. officinalis ) at 10–100 μg/mL. Quantitative data are the mean ± standard deviation. Different letters (a–c) comparing the treatments indicate significant differences ( p
Figure Legend Snippet: Results of intracellular ROS measurement in A549 ( A ) and IMR90 ( B ) cells by spectrofluoremetry. Treatment = lyophilized marigold extract ( C. officinalis ) at 10–100 μg/mL. Quantitative data are the mean ± standard deviation. Different letters (a–c) comparing the treatments indicate significant differences ( p

Techniques Used: Standard Deviation

32) Product Images from "SELDI-TOF-MS ProteinChip array profiling of T-cell clones propagated in long-term culture identifies human profilin-1 as a potential bio-marker of immunosenescence"

Article Title: SELDI-TOF-MS ProteinChip array profiling of T-cell clones propagated in long-term culture identifies human profilin-1 as a potential bio-marker of immunosenescence

Journal: Proteome Science

doi: 10.1186/1477-5956-5-7

Comparative m/z determination of the 14.9 kDa differentially expressed protein/peptide peak . Lysates from several clones were pooled and enriched under the same reversed-phase conditions that were used in the H50 ProteinChip SELDI analysis. Reversed-phase high-performance liquid chromatography (HPLC) was performed in order to compare mass obtained from SELDI analysis to more accurate MALDI-TOF MS. HPLC-purified samples were run in parallel on SELDI-TOF (on a normal-phase ProteinChip) and MALDI-TOF mass spectrometers. One peak of 14.9 kDa identified as differentially expressed in T-cell senescence is demonstrated by MALDI-TOF (a) and SELDI-TOF (b) mass spectrometric analysis.
Figure Legend Snippet: Comparative m/z determination of the 14.9 kDa differentially expressed protein/peptide peak . Lysates from several clones were pooled and enriched under the same reversed-phase conditions that were used in the H50 ProteinChip SELDI analysis. Reversed-phase high-performance liquid chromatography (HPLC) was performed in order to compare mass obtained from SELDI analysis to more accurate MALDI-TOF MS. HPLC-purified samples were run in parallel on SELDI-TOF (on a normal-phase ProteinChip) and MALDI-TOF mass spectrometers. One peak of 14.9 kDa identified as differentially expressed in T-cell senescence is demonstrated by MALDI-TOF (a) and SELDI-TOF (b) mass spectrometric analysis.

Techniques Used: Clone Assay, High Performance Liquid Chromatography, Mass Spectrometry, Purification

33) Product Images from "Anti-Acne Activity of Italian Medicinal Plants Used for Skin Infection"

Article Title: Anti-Acne Activity of Italian Medicinal Plants Used for Skin Infection

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2016.00425

The most active extracts were examined for mammalian cytotoxicity against an immortalized line of human keratinocytes (HaCaT) . Data are reported as percent cytotoxicity and the vehicle (DMSO) was included as a control. Extract numbers correspond as follows: (A) 195 : Juglans regia (EtOH extract of woody parts); 246 : Asphodelus microcarpus (MeOH extract of leaves). (B) 252 : Castanea sativa (MeOH extract of woody parts); and 275 : Juglans regia (MeOH extract of woody parts). (C) Extract numbers: 311 : Rosmarinus officinalis (MeOH extract of aerial parts: leaves, stems, flowers); 336 : Vitis vinifera var. aglianico (MeOH extract of leaves). (D) 357 : Vicia sativa subsp. sativa (MeOH extract of aerial parts: leaves, stems, flowers); and 516 : Hapalopilus rutilans (MeOH extract of fruiting bodies). Castanea sativa leaf extracts were found to be non-toxic at the tested range and this data is reported in a previous work (Quave et al., 2015 ).
Figure Legend Snippet: The most active extracts were examined for mammalian cytotoxicity against an immortalized line of human keratinocytes (HaCaT) . Data are reported as percent cytotoxicity and the vehicle (DMSO) was included as a control. Extract numbers correspond as follows: (A) 195 : Juglans regia (EtOH extract of woody parts); 246 : Asphodelus microcarpus (MeOH extract of leaves). (B) 252 : Castanea sativa (MeOH extract of woody parts); and 275 : Juglans regia (MeOH extract of woody parts). (C) Extract numbers: 311 : Rosmarinus officinalis (MeOH extract of aerial parts: leaves, stems, flowers); 336 : Vitis vinifera var. aglianico (MeOH extract of leaves). (D) 357 : Vicia sativa subsp. sativa (MeOH extract of aerial parts: leaves, stems, flowers); and 516 : Hapalopilus rutilans (MeOH extract of fruiting bodies). Castanea sativa leaf extracts were found to be non-toxic at the tested range and this data is reported in a previous work (Quave et al., 2015 ).

Techniques Used:

34) Product Images from "Effects of Thawing and Frying Methods on the Formation of Acrylamide and Polycyclic Aromatic Hydrocarbons in Chicken Meat"

Article Title: Effects of Thawing and Frying Methods on the Formation of Acrylamide and Polycyclic Aromatic Hydrocarbons in Chicken Meat

Journal: Foods

doi: 10.3390/foods9050573

Typical high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) chromatograms for the acrylamide standard (100 μg/kg) and the internal standard (20 μg/kg) in deep-fat-fried chicken wing samples after thawing by water immersion.
Figure Legend Snippet: Typical high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) chromatograms for the acrylamide standard (100 μg/kg) and the internal standard (20 μg/kg) in deep-fat-fried chicken wing samples after thawing by water immersion.

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Tandem Mass Spectroscopy

35) Product Images from "Involvement of Endocytosis in the Transdermal Penetration Mechanism of Ketoprofen Nanoparticles"

Article Title: Involvement of Endocytosis in the Transdermal Penetration Mechanism of Ketoprofen Nanoparticles

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms19072138

Mechanism for the percutaneous absorption process from the transdermal formulation containing ketoprofen nanoparticles.
Figure Legend Snippet: Mechanism for the percutaneous absorption process from the transdermal formulation containing ketoprofen nanoparticles.

Techniques Used:

Transdermal penetration of ketoprofen released from the KET-NPs formulation at 4 °C and 37 °C. ( A ) Penetration of the KET-NPs formulation through skin containing SC (normal skin); ( B ) Penetration of the KET-NPs formulation through skin from which the SC was removed (SC-removed skin); ( C ) Amount of ketoprofen in rat skin 24 h after treatment the KET-NPs formulation at 4 °C and 37 °C. The SC was removed by tape stripping. The KET-NPs formulation was applied to skin with (normal skin) or without SC (SC-removed skin). Mean ± S.E. n = 6. * p
Figure Legend Snippet: Transdermal penetration of ketoprofen released from the KET-NPs formulation at 4 °C and 37 °C. ( A ) Penetration of the KET-NPs formulation through skin containing SC (normal skin); ( B ) Penetration of the KET-NPs formulation through skin from which the SC was removed (SC-removed skin); ( C ) Amount of ketoprofen in rat skin 24 h after treatment the KET-NPs formulation at 4 °C and 37 °C. The SC was removed by tape stripping. The KET-NPs formulation was applied to skin with (normal skin) or without SC (SC-removed skin). Mean ± S.E. n = 6. * p

Techniques Used: Stripping Membranes

Effect of endocytosis on the transdermal penetration of ketoprofen released from the KET-NPs formulation. ( A ) Effect of endocytosis inhibitors (nystatin, dynasore, rottlerin and cytochalasin D) on the penetration from the KET-NPs formulation through the skin; ( B ) Changes in the transdermal penetration in the KET-NPs formulation by multi-treatment with three inhibitors (nystatin, dynasore and rottlerin; Nys + Dyn + Rot); ( C ) Effect of endocytosis inhibitors (nystatin, dynasore, rottlerin and cytochalasin D) on the amount of ketoprofen in rat skin 24 h after application of the KET-NPs formulation; ( D ) Changes in drug accumulation from the KET-NPs formulation by multi-treatment with three inhibitors (nystatin, dynasore, rottlerin; Nys + Dyn + Rot). The ketoprofen concentration in rat skin was measured 24 h after application of the KE-NPs formulation. Mean ± S.E. n = 6–8. * p
Figure Legend Snippet: Effect of endocytosis on the transdermal penetration of ketoprofen released from the KET-NPs formulation. ( A ) Effect of endocytosis inhibitors (nystatin, dynasore, rottlerin and cytochalasin D) on the penetration from the KET-NPs formulation through the skin; ( B ) Changes in the transdermal penetration in the KET-NPs formulation by multi-treatment with three inhibitors (nystatin, dynasore and rottlerin; Nys + Dyn + Rot); ( C ) Effect of endocytosis inhibitors (nystatin, dynasore, rottlerin and cytochalasin D) on the amount of ketoprofen in rat skin 24 h after application of the KET-NPs formulation; ( D ) Changes in drug accumulation from the KET-NPs formulation by multi-treatment with three inhibitors (nystatin, dynasore, rottlerin; Nys + Dyn + Rot). The ketoprofen concentration in rat skin was measured 24 h after application of the KE-NPs formulation. Mean ± S.E. n = 6–8. * p

Techniques Used: Concentration Assay

Particle size frequencies ( A ); SPM images ( B ) and solubility ( C ) of ketoprofen particles in the KET-NPs formulation. Mean ± S.E. n = 6. * p
Figure Legend Snippet: Particle size frequencies ( A ); SPM images ( B ) and solubility ( C ) of ketoprofen particles in the KET-NPs formulation. Mean ± S.E. n = 6. * p

Techniques Used: Solubility

Effect of endocytosis on the percutaneous absorption of ketoprofen released from the KET-NPs formulation. ( A ) Changes in percutaneous absorption from the KET-NPs formulation by multi-treatment with three inhibitors (nystatin, dynasore and rottlerin; Nys + Dyn + Rot); ( B ) Effect of multi-treatment with three inhibitors (nystatin, dynasore and rottlerin; Nys + Dyn + Rot) on AUC Plasma in rats treated with the KET-NPs formulation. Mean ± S.E. n = 6. * p
Figure Legend Snippet: Effect of endocytosis on the percutaneous absorption of ketoprofen released from the KET-NPs formulation. ( A ) Changes in percutaneous absorption from the KET-NPs formulation by multi-treatment with three inhibitors (nystatin, dynasore and rottlerin; Nys + Dyn + Rot); ( B ) Effect of multi-treatment with three inhibitors (nystatin, dynasore and rottlerin; Nys + Dyn + Rot) on AUC Plasma in rats treated with the KET-NPs formulation. Mean ± S.E. n = 6. * p

Techniques Used:

Ketoprofen release from the KET-NPs formulation through 25 nm and 450 nm pore membranes. ( A ) Drug release from the KET-NPs formulation through the membranes; ( B ) Number of ketoprofen particles released from the KET-NPs formulation; ( C ) Particle size frequencies of ketoprofen released from the KET-NPs formulation 24 h after application in the 450 nm pore membrane. The ketoprofen in the Franz diffusion cell (reservoir chamber filled with purified water) after the application of the KET-NPs formulation was measured by HPLC, and the number of particles was counted using NANOSIGHT LM10. Means ± S.E. n = 6. N.D., not detectable. * p
Figure Legend Snippet: Ketoprofen release from the KET-NPs formulation through 25 nm and 450 nm pore membranes. ( A ) Drug release from the KET-NPs formulation through the membranes; ( B ) Number of ketoprofen particles released from the KET-NPs formulation; ( C ) Particle size frequencies of ketoprofen released from the KET-NPs formulation 24 h after application in the 450 nm pore membrane. The ketoprofen in the Franz diffusion cell (reservoir chamber filled with purified water) after the application of the KET-NPs formulation was measured by HPLC, and the number of particles was counted using NANOSIGHT LM10. Means ± S.E. n = 6. N.D., not detectable. * p

Techniques Used: Diffusion-based Assay, Purification, High Performance Liquid Chromatography

36) Product Images from "Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine"

Article Title: Quantitation of DNA by nuclease P1 digestion and UPLC-MS/MS to assess binding efficiency of pyrrolobenzodiazepine

Journal: Journal of Pharmaceutical Analysis

doi: 10.1016/j.jpha.2020.05.011

Representative chromatograms in UPLC-MSMS analysis: (A) 20 ng/mL equimolar mixture of the 4 deoxyribonucleotides; (B) 20 ng/mL CT DNA after nuclease P1 digestion; (C) 100 μg/mL CT DNA spiked with 100 μg/mL ribonucleotide equimolar mixture, after nuclease P1 digestion (200-fold diluted before injection); (D) DNA isolated from mouse tumor, after nuclease P1 digestion (200-fold diluted before injection); (E) 20 ng/mL CT DNA after DNase I digestion.
Figure Legend Snippet: Representative chromatograms in UPLC-MSMS analysis: (A) 20 ng/mL equimolar mixture of the 4 deoxyribonucleotides; (B) 20 ng/mL CT DNA after nuclease P1 digestion; (C) 100 μg/mL CT DNA spiked with 100 μg/mL ribonucleotide equimolar mixture, after nuclease P1 digestion (200-fold diluted before injection); (D) DNA isolated from mouse tumor, after nuclease P1 digestion (200-fold diluted before injection); (E) 20 ng/mL CT DNA after DNase I digestion.

Techniques Used: Injection, Isolation

37) Product Images from "Intestinal and Hepatic Expression of Cytochrome P450s and mdr1a in Rats with Indomethacin-Induced Small Intestinal Ulcers"

Article Title: Intestinal and Hepatic Expression of Cytochrome P450s and mdr1a in Rats with Indomethacin-Induced Small Intestinal Ulcers

Journal: International Journal of Medical Sciences

doi: 10.7150/ijms.9866

Plasma concentration of VCM in rats with INM-induced SIUs. VCM was administered intraduodenally at a dose of 500 mg/kg after treatment with INM or vehicle. Venous blood was collected at 10, 20, 30, 45, 60, 90, and 120 min after the administration of VCM. VCM plasma concentration-time profiles were constructed from concentrations measured by HPLC, as described in Materials and Methods. Data were obtained for each individual rat in the control (open symbols; N = 4) and SIU (closed symbols; N = 5) groups.
Figure Legend Snippet: Plasma concentration of VCM in rats with INM-induced SIUs. VCM was administered intraduodenally at a dose of 500 mg/kg after treatment with INM or vehicle. Venous blood was collected at 10, 20, 30, 45, 60, 90, and 120 min after the administration of VCM. VCM plasma concentration-time profiles were constructed from concentrations measured by HPLC, as described in Materials and Methods. Data were obtained for each individual rat in the control (open symbols; N = 4) and SIU (closed symbols; N = 5) groups.

Techniques Used: Concentration Assay, Construct, High Performance Liquid Chromatography

38) Product Images from "Exogenous cysteine and cystine promote cell proliferation in CaCo‐2 cells"

Article Title: Exogenous cysteine and cystine promote cell proliferation in CaCo‐2 cells

Journal: Cell Proliferation

doi: 10.1046/j.1365-2184.2002.00229.x

High performance liquid chromatography separation of cystine, cysteine, GSH and GSSG in extracts of CaCo‐2 cells. (a) Separation of standard mixtures. (b) Separation of cystine, cysteine, GSH and GSSG in cell extracts. The numbers 1–4 represent the cystine, cysteine, GSH, and GSSG peaks with retention times of 12, 19, 25 and 27 min, respectively.
Figure Legend Snippet: High performance liquid chromatography separation of cystine, cysteine, GSH and GSSG in extracts of CaCo‐2 cells. (a) Separation of standard mixtures. (b) Separation of cystine, cysteine, GSH and GSSG in cell extracts. The numbers 1–4 represent the cystine, cysteine, GSH, and GSSG peaks with retention times of 12, 19, 25 and 27 min, respectively.

Techniques Used: High Performance Liquid Chromatography

39) Product Images from "Enhanced Biotransformation of Fluoranthene by Intertidally Derived Cunninghamella elegans under Biofilm-Based and Niche-Mimicking Conditions"

Article Title: Enhanced Biotransformation of Fluoranthene by Intertidally Derived Cunninghamella elegans under Biofilm-Based and Niche-Mimicking Conditions

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.02129-13

Dissolved oxygen and medium pH profiles during fluoranthene biotransformation by C. elegans in the rotating disc bioreactor. Filled circles, pO 2 in process mimicking the intertidal conditions; filled squares, pO 2 in submerged process without disc rotation;
Figure Legend Snippet: Dissolved oxygen and medium pH profiles during fluoranthene biotransformation by C. elegans in the rotating disc bioreactor. Filled circles, pO 2 in process mimicking the intertidal conditions; filled squares, pO 2 in submerged process without disc rotation;

Techniques Used:

40) Product Images from "Synthesis, Spectral Characterization, and In Vitro Cellular Activities of Metapristone, a Potential Cancer Metastatic Chemopreventive Agent Derived from Mifepristone (RU486)"

Article Title: Synthesis, Spectral Characterization, and In Vitro Cellular Activities of Metapristone, a Potential Cancer Metastatic Chemopreventive Agent Derived from Mifepristone (RU486)

Journal: The AAPS Journal

doi: 10.1208/s12248-013-9559-2

The molecular docking of mifepristone and metapristone with glucocorticoid receptors (PDB ID: 1NHZ). The purple structures representing either mifepristone (2-methyl) or metapristone (1-methyl) embedded in the active centers of the receptors linked by
Figure Legend Snippet: The molecular docking of mifepristone and metapristone with glucocorticoid receptors (PDB ID: 1NHZ). The purple structures representing either mifepristone (2-methyl) or metapristone (1-methyl) embedded in the active centers of the receptors linked by

Techniques Used:

Related Articles

High Performance Liquid Chromatography:

Article Title: Neurophysiological and Neurochemical Effects of the Putative Cognitive Enhancer (S)-CE-123 on Mesocorticolimbic Dopamine System
Article Snippet: .. CE-123 was further separated into individual enantiomers (S - and R-CE-123, respectively) using a Shimadzu 10AVP HPLC System (Shimadzu Corporation, Tokyo, Japan) equipped with Chiralpak IA semipreparative column (10 mm diameter× 20 cm length) (Chiral Technologies Europe, France). .. R -modafinil, (R)-2-(benzhydrylsulfinyl)acetamide was also synthesized in house as reported previously [ ] via a procedure based on the US 7,812,193 B2 patent application.

Article Title: Gcg-XTEN: An Improved Glucagon Capable of Preventing Hypoglycemia without Increasing Baseline Blood Glucose
Article Snippet: .. Reverse-Phase Chromatography Reverse phase C18 chromatography (RPC18) was performed using a Phenomenex Gemini 5 µm C18, 100 Å, 4.6×100 mm column (Phenomenex) connected to an HPLC system equipped with an autosampler and UV/VIS detector (Shimadzu). ..

Article Title: Isolation of protein N-terminal peptides by charge-mediated position-selective enrichment using strong cation exchange chromatography
Article Snippet: .. Peptide fractionation by SCX HPLCSCX chromatography was performed using a Prominence HPLC system (Shimadzu, Kyoto, Japan) with a BioIEX SCX column (250 mm × 4.6 mm inner diameter, 5 μm non-porous beads made of poly(styrene-divinylbenzene) modified with sulfonate groups (Agilent, Santa Clara, CA, USA). ..

Article Title: Determination of Tangeretin in Rat Plasma: Assessment of Its Clearance and Absolute Oral Bioavailability
Article Snippet: .. The stability of TAN in Sprague-Dawley rat plasma under the conditions that might be encountered during sample collection, storage, processing and HPLC analysis was investigated at low QC, medium QC and high QC concentrations. ..

Article Title: Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity
Article Snippet: .. A 7 min linear elution gradient was used: ramp to 40% B at 1 min, then to 100% B at 5 min, hold at 100% B to 6 min, return to 90% A at 6.01 min and hold until 7 min. For analyzing trichome acylsugars extracted from S. pennellii transgenic plants and membrane lipids from N. benthamiana , a Shimadzu LC-20AD HPLC system connected to a Waters Xevo G2-XS QToF mass spectrometer was used. ..

Article Title: Administration of a Decoction of Sucrose- and Polysaccharide-Rich Radix Astragali (Huang Qi) Ameliorated Insulin Resistance and Fatty Liver but Affected Beta-Cell Function in Type 2 Diabetic Rats
Article Snippet: .. Chemical Analysis by High-Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) By employing reference compounds including astragaloside I and II, isoastragaloside I, and sucrose, the AM-W and AM-E were analyzed using the following apparatus and conditions: the HPLC profile was obtained from a Shimadzu 10AVP series system equipped with two pumps (LC-10ADVP, Shimadzu, Kyoto, Japan), a column oven (CTO-10ASVP, Shimadzu), an evaporative light scattering detector (ELSD) (ELSD-LT II, Shimadzu), a two-channel vacuum degasser (model 2003, Biotech AB, Onsala, Sweden), an SIL-10AVP automatic injector, an SCL-10AVP system controller, and a class VP workstation for data analysis. ..

Chromatography:

Article Title: Gcg-XTEN: An Improved Glucagon Capable of Preventing Hypoglycemia without Increasing Baseline Blood Glucose
Article Snippet: .. Reverse-Phase Chromatography Reverse phase C18 chromatography (RPC18) was performed using a Phenomenex Gemini 5 µm C18, 100 Å, 4.6×100 mm column (Phenomenex) connected to an HPLC system equipped with an autosampler and UV/VIS detector (Shimadzu). ..

Article Title: Isolation of protein N-terminal peptides by charge-mediated position-selective enrichment using strong cation exchange chromatography
Article Snippet: .. Peptide fractionation by SCX HPLCSCX chromatography was performed using a Prominence HPLC system (Shimadzu, Kyoto, Japan) with a BioIEX SCX column (250 mm × 4.6 mm inner diameter, 5 μm non-porous beads made of poly(styrene-divinylbenzene) modified with sulfonate groups (Agilent, Santa Clara, CA, USA). ..

Peptide Fractionation:

Article Title: Isolation of protein N-terminal peptides by charge-mediated position-selective enrichment using strong cation exchange chromatography
Article Snippet: .. Peptide fractionation by SCX HPLCSCX chromatography was performed using a Prominence HPLC system (Shimadzu, Kyoto, Japan) with a BioIEX SCX column (250 mm × 4.6 mm inner diameter, 5 μm non-porous beads made of poly(styrene-divinylbenzene) modified with sulfonate groups (Agilent, Santa Clara, CA, USA). ..

Transgenic Assay:

Article Title: Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity
Article Snippet: .. A 7 min linear elution gradient was used: ramp to 40% B at 1 min, then to 100% B at 5 min, hold at 100% B to 6 min, return to 90% A at 6.01 min and hold until 7 min. For analyzing trichome acylsugars extracted from S. pennellii transgenic plants and membrane lipids from N. benthamiana , a Shimadzu LC-20AD HPLC system connected to a Waters Xevo G2-XS QToF mass spectrometer was used. ..

Nuclear Magnetic Resonance:

Article Title: Administration of a Decoction of Sucrose- and Polysaccharide-Rich Radix Astragali (Huang Qi) Ameliorated Insulin Resistance and Fatty Liver but Affected Beta-Cell Function in Type 2 Diabetic Rats
Article Snippet: .. Chemical Analysis by High-Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) By employing reference compounds including astragaloside I and II, isoastragaloside I, and sucrose, the AM-W and AM-E were analyzed using the following apparatus and conditions: the HPLC profile was obtained from a Shimadzu 10AVP series system equipped with two pumps (LC-10ADVP, Shimadzu, Kyoto, Japan), a column oven (CTO-10ASVP, Shimadzu), an evaporative light scattering detector (ELSD) (ELSD-LT II, Shimadzu), a two-channel vacuum degasser (model 2003, Biotech AB, Onsala, Sweden), an SIL-10AVP automatic injector, an SCL-10AVP system controller, and a class VP workstation for data analysis. ..

other:

Article Title: Accurate quantitation standards of glutathione via traceable sulfur measurement by inductively coupled plasma optical emission spectrometry and ion chromatography
Article Snippet: The residual carbon content (RCC) of GSH digest was measured using a TOC analyzer (TOC-VCPN 5000A, Shimadzu, Japan).

Mass Spectrometry:

Article Title: Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry
Article Snippet: .. The sample suspension was precipitated by the methanol/chloroform method and analysed by MALDI-TOF MS of secreted SE proteins, as described above. .. This sample preparation protocol of foodborne SEB was optimal, and no laborious steps like isoelectric precipitation for the removal of casein proteins of milk were included.

Article Title: Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity
Article Snippet: .. A 7 min linear elution gradient was used: ramp to 40% B at 1 min, then to 100% B at 5 min, hold at 100% B to 6 min, return to 90% A at 6.01 min and hold until 7 min. For analyzing trichome acylsugars extracted from S. pennellii transgenic plants and membrane lipids from N. benthamiana , a Shimadzu LC-20AD HPLC system connected to a Waters Xevo G2-XS QToF mass spectrometer was used. ..

Modification:

Article Title: Isolation of protein N-terminal peptides by charge-mediated position-selective enrichment using strong cation exchange chromatography
Article Snippet: .. Peptide fractionation by SCX HPLCSCX chromatography was performed using a Prominence HPLC system (Shimadzu, Kyoto, Japan) with a BioIEX SCX column (250 mm × 4.6 mm inner diameter, 5 μm non-porous beads made of poly(styrene-divinylbenzene) modified with sulfonate groups (Agilent, Santa Clara, CA, USA). ..

Reversed-phase Chromatography:

Article Title: Gcg-XTEN: An Improved Glucagon Capable of Preventing Hypoglycemia without Increasing Baseline Blood Glucose
Article Snippet: .. Reverse-Phase Chromatography Reverse phase C18 chromatography (RPC18) was performed using a Phenomenex Gemini 5 µm C18, 100 Å, 4.6×100 mm column (Phenomenex) connected to an HPLC system equipped with an autosampler and UV/VIS detector (Shimadzu). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89
    Shimadzu Corporation high performance liquid chromatography hplc conditions chromatographic separation
    High Performance Liquid Chromatography Hplc Conditions Chromatographic Separation, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography hplc conditions chromatographic separation/product/Shimadzu Corporation
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatography hplc conditions chromatographic separation - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    88
    Shimadzu Corporation hplc ms ms conditions uflc xr ultra high performance liquid chromatograph
    Hplc Ms Ms Conditions Uflc Xr Ultra High Performance Liquid Chromatograph, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc ms ms conditions uflc xr ultra high performance liquid chromatograph/product/Shimadzu Corporation
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc ms ms conditions uflc xr ultra high performance liquid chromatograph - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    86
    Shimadzu Corporation high performance liquid chromatagraphy hplc conditions hplc
    High Performance Liquid Chromatagraphy Hplc Conditions Hplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatagraphy hplc conditions hplc/product/Shimadzu Corporation
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatagraphy hplc conditions hplc - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    88
    Shimadzu Corporation high performance liquid chromatography conditions high performance liquid chromatography hplc
    High Performance Liquid Chromatography Conditions High Performance Liquid Chromatography Hplc, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography conditions high performance liquid chromatography hplc/product/Shimadzu Corporation
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatography conditions high performance liquid chromatography hplc - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    Image Search Results