high fidelity phusion flash dna polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher high fidelity phusion flash dna polymerase
    High Fidelity Phusion Flash Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high fidelity phusion flash dna polymerase/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    high fidelity phusion flash dna polymerase - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection
    Article Snippet: .. VapC-1 D6N and D6N D99N were cloned by amplifying the vapC-1 D6N gene from pDD757 and the vapC-1 D6N D99N gene from pDD1118, using high-fidelity Phusion Flash DNA Polymerase with pETD6NC1SacFor and 322LexRev primers. .. The PCR products, pSR660, and pSR661 were digested with SacI and KpnI prior to ligation, resulting in pDD1125, pDD1126, pDD1129, and pDD1128.

    Article Title: Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection
    Article Snippet: .. The VapC-1 D99N, E43Q, E43Q D99N, and E120Q mutants were cloned by amplifying the vapC-1 D99N gene from pDD758, the vapC-1 E43Q gene from pDD1165, the vapC-1 E43Q D99N gene from pDD1196, or the vapC-1 E120Q gene from pDD1166 using high-fidelity Phusion Flash DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA) and the primers 322LexFor and 322LexRev. .. The PCR products, pSR658, and pSR659 were digested with SacI and KpnI prior to ligation, resulting in pDD1120, pDD1123, pDD1170, pDD1178, pDD1199, pDD1200, pDD1177, and pDD1179.

    Amplification:

    Article Title: Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection
    Article Snippet: .. The vapB-1 gene was amplified from 86-028NP genomic DNA using high-fidelity Phusion Flash DNA polymerase with 321LexFor and 321LexRev primers. .. After digestion with SacI and KpnI the PCR fragment was ligated into pTrcHisA, resulting in pDD935.

    Article Title: Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection
    Article Snippet: .. The second homologous region of 767 bp of the 3′ end of NTHI_RS09270 was amplified from 86-028NP genomic DNA using high-fidelity Phusion Flash DNA polymerase with 2009-2XhoFor and 2009-2KpnRev primers and ligated into pDD1058 cut with XhoI and KpnI, resulting in pDD1063. .. The last step was amplifying an erythromycin resistance cassette from pEJ18 ( ) with PermCSpeFor and ErmCSpeRev primers and ligating into pDD1063 cut with SpeI, resulting in the delivery vector, pDD1140.

    Ligation:

    Article Title: Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection
    Article Snippet: The VapC-1 D99N, E43Q, E43Q D99N, and E120Q mutants were cloned by amplifying the vapC-1 D99N gene from pDD758, the vapC-1 E43Q gene from pDD1165, the vapC-1 E43Q D99N gene from pDD1196, or the vapC-1 E120Q gene from pDD1166 using high-fidelity Phusion Flash DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA) and the primers 322LexFor and 322LexRev. .. The PCR products, pSR658, and pSR659 were digested with SacI and KpnI prior to ligation, resulting in pDD1120, pDD1123, pDD1170, pDD1178, pDD1199, pDD1200, pDD1177, and pDD1179.

    Construct:

    Article Title: Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection
    Article Snippet: .. The delivery vector was constructed by amplifying 843 bp of the 5′ end of NTHI_RS09270 from 86-028NP genomic DNA using high-fidelity Phusion Flash DNA polymerase with 2009-1SacFor and 2009-1XbaRev primers and ligating the product into pBluescript SK+ digested with SacI and XbaI, resulting in pDD1058. .. The second homologous region of 767 bp of the 3′ end of NTHI_RS09270 was amplified from 86-028NP genomic DNA using high-fidelity Phusion Flash DNA polymerase with 2009-2XhoFor and 2009-2KpnRev primers and ligated into pDD1058 cut with XhoI and KpnI, resulting in pDD1063.

    Polymerase Chain Reaction:

    Article Title: Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection
    Article Snippet: The VapC-1 D99N, E43Q, E43Q D99N, and E120Q mutants were cloned by amplifying the vapC-1 D99N gene from pDD758, the vapC-1 E43Q gene from pDD1165, the vapC-1 E43Q D99N gene from pDD1196, or the vapC-1 E120Q gene from pDD1166 using high-fidelity Phusion Flash DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA) and the primers 322LexFor and 322LexRev. .. The PCR products, pSR658, and pSR659 were digested with SacI and KpnI prior to ligation, resulting in pDD1120, pDD1123, pDD1170, pDD1178, pDD1199, pDD1200, pDD1177, and pDD1179.

    Plasmid Preparation:

    Article Title: Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection
    Article Snippet: .. The delivery vector was constructed by amplifying 843 bp of the 5′ end of NTHI_RS09270 from 86-028NP genomic DNA using high-fidelity Phusion Flash DNA polymerase with 2009-1SacFor and 2009-1XbaRev primers and ligating the product into pBluescript SK+ digested with SacI and XbaI, resulting in pDD1058. .. The second homologous region of 767 bp of the 3′ end of NTHI_RS09270 was amplified from 86-028NP genomic DNA using high-fidelity Phusion Flash DNA polymerase with 2009-2XhoFor and 2009-2KpnRev primers and ligated into pDD1058 cut with XhoI and KpnI, resulting in pDD1063.

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  • 99
    Thermo Fisher high fidelity phusion flash dna polymerase
    High Fidelity Phusion Flash Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high fidelity phusion flash dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    high fidelity phusion flash dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
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