high capacity cdna reverse transcription kit reagents  (Thermo Fisher)


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    Name:
    High Capacity cDNA Reverse Transcription Kit
    Description:
    The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single stranded cDNA in a single 20 µL reaction The High Capacity cDNA Reverse Transcription Kit delivers extremely high quality single stranded cDNA from 0 02 to 2 µg total RNA Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction Downstream applications include real time PCR standard PCR and microarrays The kit is ideal for generating cDNA archives Features of this kit include • Linear target amplification for real time PCR• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost• 10 fold greater dynamic range than other kitsExtensively tested with a variety of templates Quantitative first strand synthesis of all RNA species is achieved with the use of the random primers The kit has been extensively tested with a variety of RNA templates including GC and AU rich targets and RNAs expressed at low levels cRNA can also be efficiently generated from in vitro transcription of cDNA
    Catalog Number:
    4368813
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher high capacity cdna reverse transcription kit reagents
    The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single stranded cDNA in a single 20 µL reaction The High Capacity cDNA Reverse Transcription Kit delivers extremely high quality single stranded cDNA from 0 02 to 2 µg total RNA Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction Downstream applications include real time PCR standard PCR and microarrays The kit is ideal for generating cDNA archives Features of this kit include • Linear target amplification for real time PCR• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost• 10 fold greater dynamic range than other kitsExtensively tested with a variety of templates Quantitative first strand synthesis of all RNA species is achieved with the use of the random primers The kit has been extensively tested with a variety of RNA templates including GC and AU rich targets and RNAs expressed at low levels cRNA can also be efficiently generated from in vitro transcription of cDNA
    https://www.bioz.com/result/high capacity cdna reverse transcription kit reagents/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    high capacity cdna reverse transcription kit reagents - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: K562 chronic myeloid leukemia cells modify osteogenic differentiation and gene expression of bone marrow stromal cells
    Article Snippet: .. The resulting RNA was reverse transcribed using high capacity cDNA synthesis kit and oligodT primers at 37°C for 120 min. Real-time PCR was performed using Power SyBr Green reagents in an ABI 7500 system (Thermo Fisher Scientific). .. The gene expression levels in each sample was normalized to their respective GAPDH expression levels.

    Isolation:

    Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
    Article Snippet: .. In brief, total RNA was isolated and cDNA synthesis was performed with 500 ng of RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers (AnxA1: forward 5’-AATCCATCCTCGGATGTCGC-3’ , nt 335–354; reverse 5’-TCAGTGTTTCATCCAGGGGC-3’ , nt 478–497; AnxA6: forward 5’-TGGCTGCTGAGATCTTGGAAA-3’ , nt 1679–1699; reverse 5’-GATCGTCATGAAACGTGTCTCC-3’ , nt 1734–1755; PDK4: forward 5’-TCAGACAGAGGAGGTGGTGTT-3’ , nt 1194–2114; reverse 5’-AAAACCAGCCAAAGGAGCATTC , nt 2189–3110; 28s rRNA: forward 5’-GTTGTTGCCATGGTAATCCTGCTCAGTAC-3 ’, nt 4535–4564, reverse 5’-TCTGACTTAGAGGCGTTCAGTCATAATCC-3’ , nt 4638–4667) following the manufacturer's instructions. ..

    Random Hexamer Labeling:

    Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
    Article Snippet: .. In brief, total RNA was isolated and cDNA synthesis was performed with 500 ng of RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers (AnxA1: forward 5’-AATCCATCCTCGGATGTCGC-3’ , nt 335–354; reverse 5’-TCAGTGTTTCATCCAGGGGC-3’ , nt 478–497; AnxA6: forward 5’-TGGCTGCTGAGATCTTGGAAA-3’ , nt 1679–1699; reverse 5’-GATCGTCATGAAACGTGTCTCC-3’ , nt 1734–1755; PDK4: forward 5’-TCAGACAGAGGAGGTGGTGTT-3’ , nt 1194–2114; reverse 5’-AAAACCAGCCAAAGGAGCATTC , nt 2189–3110; 28s rRNA: forward 5’-GTTGTTGCCATGGTAATCCTGCTCAGTAC-3 ’, nt 4535–4564, reverse 5’-TCTGACTTAGAGGCGTTCAGTCATAATCC-3’ , nt 4638–4667) following the manufacturer's instructions. ..

    Construct:

    Article Title: Tissue, developmental, and caste-specific expression of odorant binding proteins in a eusocial insect, the red imported fire ant, Solenopsis invicta
    Article Snippet: .. Finally, 2 μg total RNA from different tissues were used to construct cDNA libraries using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) in terms of standard protocol. .. Absolute qualification of cDNAs The sequences of the S. invicta odorant binding proteins (SiOBP s) were downloaded from NCBI ( ).

    SYBR Green Assay:

    Article Title: K562 chronic myeloid leukemia cells modify osteogenic differentiation and gene expression of bone marrow stromal cells
    Article Snippet: .. The resulting RNA was reverse transcribed using high capacity cDNA synthesis kit and oligodT primers at 37°C for 120 min. Real-time PCR was performed using Power SyBr Green reagents in an ABI 7500 system (Thermo Fisher Scientific). .. The gene expression levels in each sample was normalized to their respective GAPDH expression levels.

    Concentration Assay:

    Article Title: Comprehensive evaluation of blood-brain barrier-forming micro-vasculatures: Reference and marker genes with cellular composition
    Article Snippet: .. Extracted total RNA was reverse transcribed at a concentration of 25 ng/μL using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) according to the manufacturer’s instructions. .. Real-time quantitative PCR The Taqman primer/probe sets for cDNA of selected reference genes and marker genes were either designed using Primer ExpressTM software (Version 1.5, ABI Prism) or ordered from Applied Biosystems (ABI, Carlsbad, CA) ( ).

    Generated:

    Article Title: TLR-4-Dependent and -Independent Mechanisms of Fetal Brain Injury in the Setting of Preterm Birth
    Article Snippet: .. Complementary DNA (cDNA) was generated from 20 µg of RNA/sample using a high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, California). .. To assess expression of neuronal markers, quantitative polymerase chain reaction (qPCR) was performed using equivalent dilutions of each sample on the Applied Biosystems Model 9700 sequence detector PCR machine, as reported previously., , Primer sets to neuronal markers MAP-2 and nestin, conjugated to Taqman MGB probes, were used for qPCR (Applied Biosystems).

    Quantitative RT-PCR:

    Article Title: Ndr kinases regulate retinal interneuron proliferation and homeostasis
    Article Snippet: .. For RT-qPCR, RNA samples were reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit following standard procedures from the manufacturer (Applied Biosystems, Foster City, CA, #4368814). .. The RT-qPCR reactions contained 40 ng cDNA, 1x SYBR Green PCR Master Mix (Applied Biosystems, #4309155), and 250 nM of each unlabeled forward and reverse primer.

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  • 99
    Thermo Fisher high capacity cdna reverse transcription kit
    High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high capacity cdna reverse transcription kit/product/Thermo Fisher
    Average 99 stars, based on 10124 article reviews
    Price from $9.99 to $1999.99
    high capacity cdna reverse transcription kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher reverse transcription kit
    Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription kit/product/Thermo Fisher
    Average 99 stars, based on 241 article reviews
    Price from $9.99 to $1999.99
    reverse transcription kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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