tri reagent  (Thermo Fisher)


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    Name:
    High Capacity cDNA Reverse Transcription Kit
    Description:
    The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single stranded cDNA in a single 20 µL reaction The High Capacity cDNA Reverse Transcription Kit delivers extremely high quality single stranded cDNA from 0 02 to 2 µg total RNA Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction Downstream applications include real time PCR standard PCR and microarrays The kit is ideal for generating cDNA archives Features of this kit include • Linear target amplification for real time PCR• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost• 10 fold greater dynamic range than other kitsExtensively tested with a variety of templates Quantitative first strand synthesis of all RNA species is achieved with the use of the random primers The kit has been extensively tested with a variety of RNA templates including GC and AU rich targets and RNAs expressed at low levels cRNA can also be efficiently generated from in vitro transcription of cDNA
    Catalog Number:
    4368813
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Reverse Transcription
    Category:
    Kits and Assays
    Buy from Supplier


    Structured Review

    Thermo Fisher tri reagent
    The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single stranded cDNA in a single 20 µL reaction The High Capacity cDNA Reverse Transcription Kit delivers extremely high quality single stranded cDNA from 0 02 to 2 µg total RNA Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction Downstream applications include real time PCR standard PCR and microarrays The kit is ideal for generating cDNA archives Features of this kit include • Linear target amplification for real time PCR• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost• 10 fold greater dynamic range than other kitsExtensively tested with a variety of templates Quantitative first strand synthesis of all RNA species is achieved with the use of the random primers The kit has been extensively tested with a variety of RNA templates including GC and AU rich targets and RNAs expressed at low levels cRNA can also be efficiently generated from in vitro transcription of cDNA
    https://www.bioz.com/result/tri reagent/product/Thermo Fisher
    Average 90 stars, based on 3948 article reviews
    Price from $9.99 to $1999.99
    tri reagent - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells
    Article Snippet: RNA isolation and quantitative-PCR for miR-101 & target gene expression RNA was isolated using TRIzol (Sigma) method and the quality and quantity verified by formaldehyde gel electrophoresis and Nanodrop (ND-1000, Nanodrop), respectively. cDNA preparation was carried out, using the High-Throughput cDNA Preparation kit (Part No-4368813, Life Technologies), following manufacturer’s protocol, using 5 ng of RNA for cDNA synthesis of microRNA. .. Target (miR-101) and reference (RNU44) genes were amplified using ABI PRISM 7000 sequence detection system (Applied Biosystems).

    Synthesized:

    Article Title: BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation
    Article Snippet: .. RT-qPCR Total RNA was isolated using Trizol-Chloroform extraction and isopropanol precipitation. cDNA was synthesized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress; Use of APE1 small molecule inhibitors to delineate APE1 functions
    Article Snippet: .. The extracted RNA was quantified by a SmartSpec spectrophotometer (Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 260 nm. cDNA was synthesized by High-Capacity cDNA Reverse Transcription Kit according to the protocol provided by the manufacture (Part No.: 4368814 Applied Biosystems). .. The primers for TNF-α were 5’-AGGCTGCCCCGACTATGTG-3’ and 5’-AGGAGGCTGACTTTCTCCTGGTA-3’; the primers for GAPDH were 5’-GTATGACTCTAC CCACGGCAAGT-3’ and 5’-TCTCGCTCCTGGAAGATGGT-3’. qRT-PCR was conducted on an ABI PRISM 7500 Sequence Detection System (Applied Biosystems) using RT2-SYBR Green /ROX qPCR Master Mix (SABiosciences, Frederick, MD) in a 25ul final reaction mixture.

    Quantitative RT-PCR:

    Article Title: Pregnancy in Obese Mice Protects Selectively against Visceral Adiposity and Is Associated with Increased Adipocyte Estrogen Signalling
    Article Snippet: .. Quantitative RT-PCR 600 ng of total RNA, from visceral adipose tissue, was reverse transcribed using the cDNA Synthesis Kit (4368813-Applied Biosystems, Carlsbad, US). .. We analysed levels of gene-specific mRNA using an ABI 7900HT (Applied Biosystems, Hill, U.K.) with inventoried probes and primer sets included in (Applied Biosystems, Hill, U.K.) and expressed values normalized against cyclophilin A mRNA levels.

    Article Title: Association between EZH2 expression, silencing of tumor suppressors and disease outcome in solid tumors
    Article Snippet: .. RT-qPCR Total RNA was isolated using TRIzol reagent (Invitrogen). cDNA was synthetized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: .. Samples were resuspended in 60μL water and 2 μg RNA from each sample were subsequently subjected to qRT-PCR using TaqMan RT-PCR ABI7900HT, in a two-step procedure, as following: 1) For reverse transcriptase step we utilized ABI High Capacity cDNA Reverse Transcription Kit (4368814,from Applied Biosystems). ..

    Article Title: Pumpkin (Cucurbita ficifolia Bouché) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression
    Article Snippet: Paragraph title: 2.5. RNA isolation and qRT-PCR gene expression analysis ... 2 μg of total RNA was reverse transcribed using a high capacity cDNA reverse transcript kit [Part No: 4368814; Applied Biosystem (ABI)] according to the manufacturer’s protocol.

    Article Title: BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation
    Article Snippet: .. RT-qPCR Total RNA was isolated using Trizol-Chloroform extraction and isopropanol precipitation. cDNA was synthesized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells
    Article Snippet: .. RT-qPCR from mouse tissues The total RNA was isolated using the Rneasy Mini Kit (Qiagen). cDNA was synthetized using High Capacity cDNA RT kit (4368814-Applied Biosystems), and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: Low-Molecular-Weight Heparin and Unfractionated Heparin Decrease Th-1, 2, and 17 Expressions
    Article Snippet: Paragraph title: Quantitative RT-PCR (qRT-PCR) analysis of mRNA transcripts ... Total RNA(5ug) was reverse-transcribed using High-Capacity cDNA Reverse Transcription kits (Cat. 4368814)(Applied Biosystems) and random primers (Applied Biosystems).

    Article Title: Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress; Use of APE1 small molecule inhibitors to delineate APE1 functions
    Article Snippet: Paragraph title: 2.8. qRT-PCR assay ... The extracted RNA was quantified by a SmartSpec spectrophotometer (Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 260 nm. cDNA was synthesized by High-Capacity cDNA Reverse Transcription Kit according to the protocol provided by the manufacture (Part No.: 4368814 Applied Biosystems).

    Real-time Polymerase Chain Reaction:

    Article Title: MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells
    Article Snippet: .. RNA isolation and quantitative-PCR for miR-101 & target gene expression RNA was isolated using TRIzol (Sigma) method and the quality and quantity verified by formaldehyde gel electrophoresis and Nanodrop (ND-1000, Nanodrop), respectively. cDNA preparation was carried out, using the High-Throughput cDNA Preparation kit (Part No-4368813, Life Technologies), following manufacturer’s protocol, using 5 ng of RNA for cDNA synthesis of microRNA. .. TaqManRNU44 and has-miR-101 assays were performed by real time PCR.

    Article Title: Association between EZH2 expression, silencing of tumor suppressors and disease outcome in solid tumors
    Article Snippet: .. RT-qPCR Total RNA was isolated using TRIzol reagent (Invitrogen). cDNA was synthetized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: Samples were resuspended in 60μL water and 2 μg RNA from each sample were subsequently subjected to qRT-PCR using TaqMan RT-PCR ABI7900HT, in a two-step procedure, as following: 1) For reverse transcriptase step we utilized ABI High Capacity cDNA Reverse Transcription Kit (4368814,from Applied Biosystems). .. For the qPCR reaction we utilized ABI 2X universal Master mix (4324018, Applied Biosystems) using ddCt (RQ) method for quantification.

    Article Title: Pumpkin (Cucurbita ficifolia Bouché) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression
    Article Snippet: 2 μg of total RNA was reverse transcribed using a high capacity cDNA reverse transcript kit [Part No: 4368814; Applied Biosystem (ABI)] according to the manufacturer’s protocol. .. The expression levels of adipogenic-related genes [ADIPOQ, FABP 4, PPARGC1A, ACACB, CEBPB, CEBPA, and LPL] were quantified using custom Taqman low density array cards (S1 Table), and ViiA 7 real-time PCR device (ABI).

    Article Title: BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation
    Article Snippet: .. RT-qPCR Total RNA was isolated using Trizol-Chloroform extraction and isopropanol precipitation. cDNA was synthesized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells
    Article Snippet: .. RT-qPCR from mouse tissues The total RNA was isolated using the Rneasy Mini Kit (Qiagen). cDNA was synthetized using High Capacity cDNA RT kit (4368814-Applied Biosystems), and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: Low-Molecular-Weight Heparin and Unfractionated Heparin Decrease Th-1, 2, and 17 Expressions
    Article Snippet: Total RNA(5ug) was reverse-transcribed using High-Capacity cDNA Reverse Transcription kits (Cat. 4368814)(Applied Biosystems) and random primers (Applied Biosystems). .. Quantitative PCR was performed by StepOne(tm) Real-Time PCR System (Applied Biosystems) with SYBR Green PCR Master Mix (Roche), and 1 ug cDNA was added to a volume of 20 uL.

    Article Title: Renal compartment-specific genetic variation analyses identify new pathways in chronic kidney disease
    Article Snippet: .. 1μg RNA was reverse transcribed using cDNA archival kit (Applied Biosystems#4368813), and quantitative real-time PCR was conducted in the ViiA 7 System (Applied Biosystems) machine using SYBRGreen Master Mix (Applied Biosystems#A25742). ..

    Article Title: A neuronal network of mitochondrial dynamics regulates metastasis
    Article Snippet: mRNA analysis Relative mRNA levels of SNPH were determined by qPCR. .. Two μg of RNA were reversed transcribed using random hexamers for 1 h at 37 °C using the TaqMan High-Capacity cDNA Reverse Transcription Kit (Life Technologies#4368814).

    Article Title: Haloperidol induces pharmacoepigenetic response by modulating miRNA expression, global DNA methylation and expression profiles of methylation maintenance genes and genes involved in neurotransmission in neuronal cells
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and real-time PCR analysis ... The total RNA was quantified using Nanodrop ND-1000 spectrophotometer and its quality was assessed by running in 1.2% agarose gel.1μg of total RNA was used for cDNA synthesis using High Capacity Reverse Transcriptase kit (Applied Biosystems Part No:4368814) as per the manufacturer’s instruction.

    Article Title: Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress; Use of APE1 small molecule inhibitors to delineate APE1 functions
    Article Snippet: The extracted RNA was quantified by a SmartSpec spectrophotometer (Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 260 nm. cDNA was synthesized by High-Capacity cDNA Reverse Transcription Kit according to the protocol provided by the manufacture (Part No.: 4368814 Applied Biosystems). .. The primers for TNF-α were 5’-AGGCTGCCCCGACTATGTG-3’ and 5’-AGGAGGCTGACTTTCTCCTGGTA-3’; the primers for GAPDH were 5’-GTATGACTCTAC CCACGGCAAGT-3’ and 5’-TCTCGCTCCTGGAAGATGGT-3’. qRT-PCR was conducted on an ABI PRISM 7500 Sequence Detection System (Applied Biosystems) using RT2-SYBR Green /ROX qPCR Master Mix (SABiosciences, Frederick, MD) in a 25ul final reaction mixture.

    Incubation:

    Article Title: Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress; Use of APE1 small molecule inhibitors to delineate APE1 functions
    Article Snippet: The extracted RNA was quantified by a SmartSpec spectrophotometer (Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 260 nm. cDNA was synthesized by High-Capacity cDNA Reverse Transcription Kit according to the protocol provided by the manufacture (Part No.: 4368814 Applied Biosystems). .. The cycling conditions were an incubation at 50°C for 2 min, 95°C for 10min and 40 cycls of 15s at 95°C, and 1 min at 60°C.

    Expressing:

    Article Title: MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells
    Article Snippet: .. RNA isolation and quantitative-PCR for miR-101 & target gene expression RNA was isolated using TRIzol (Sigma) method and the quality and quantity verified by formaldehyde gel electrophoresis and Nanodrop (ND-1000, Nanodrop), respectively. cDNA preparation was carried out, using the High-Throughput cDNA Preparation kit (Part No-4368813, Life Technologies), following manufacturer’s protocol, using 5 ng of RNA for cDNA synthesis of microRNA. .. TaqManRNU44 and has-miR-101 assays were performed by real time PCR.

    Article Title: Pumpkin (Cucurbita ficifolia Bouché) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression
    Article Snippet: Paragraph title: 2.5. RNA isolation and qRT-PCR gene expression analysis ... 2 μg of total RNA was reverse transcribed using a high capacity cDNA reverse transcript kit [Part No: 4368814; Applied Biosystem (ABI)] according to the manufacturer’s protocol.

    Article Title: Genome-Wide Bovine H3K27me3 Modifications and the Regulatory Effects on Genes Expressions in Peripheral Blood Lymphocytes
    Article Snippet: Paragraph title: Digital Gene Expression and Analysis ... Approximately 6 µg of total RNA was transcribed into double-stranded cDNA through Reverse Transcription Kit (Applied Biosystems, PN.4368814).

    Article Title: A neuronal network of mitochondrial dynamics regulates metastasis
    Article Snippet: Two μg of RNA were reversed transcribed using random hexamers for 1 h at 37 °C using the TaqMan High-Capacity cDNA Reverse Transcription Kit (Life Technologies#4368814). .. One μl of cDNA diluted 1:5 was used as template for qPCR reactions with TaqMan Gene Expression (GEX) assays to detect the various transcripts in a ABI7500 Fast Real Time PCR system.

    Article Title: Haloperidol induces pharmacoepigenetic response by modulating miRNA expression, global DNA methylation and expression profiles of methylation maintenance genes and genes involved in neurotransmission in neuronal cells
    Article Snippet: The total RNA was quantified using Nanodrop ND-1000 spectrophotometer and its quality was assessed by running in 1.2% agarose gel.1μg of total RNA was used for cDNA synthesis using High Capacity Reverse Transcriptase kit (Applied Biosystems Part No:4368814) as per the manufacturer’s instruction. .. The expression of epigenetic genes (DNMT1 , DNMT3A , DNMT3B ,MBD2 ) were analysed using real time quantification of cDNA using either Taqman gene expression assay (Applied Biosystems) or SYBR Green based assays (Applied Biosystems). ß-actin (ACTB) was used as the endogenous control.

    Article Title: Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress; Use of APE1 small molecule inhibitors to delineate APE1 functions
    Article Snippet: qRT-PCR was used to measure the mRNA expression levels of TNF-α (tumor necrosis factor-alpha) gene. .. The extracted RNA was quantified by a SmartSpec spectrophotometer (Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 260 nm. cDNA was synthesized by High-Capacity cDNA Reverse Transcription Kit according to the protocol provided by the manufacture (Part No.: 4368814 Applied Biosystems).

    Article Title: Deregulation of miR-34a and Its Chaperon Hsp70 in Hepatitis C virus-Induced Liver Cirrhosis and Hepatocellular Carcinoma Patients
    Article Snippet: .. Target gene heat shock protein-70 expression analysis High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, P/N 4368814) was used for RT reaction. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: .. Samples were resuspended in 60μL water and 2 μg RNA from each sample were subsequently subjected to qRT-PCR using TaqMan RT-PCR ABI7900HT, in a two-step procedure, as following: 1) For reverse transcriptase step we utilized ABI High Capacity cDNA Reverse Transcription Kit (4368814,from Applied Biosystems). ..

    Generated:

    Article Title: Expression Analysis of Multiple Genes May Involve in Antimony Resistance among Leishmania major Clinical Isolates from Fars Province, Central Iran
    Article Snippet: .. -cDNAs synthesis cDNAs were generated using first-strand cDNA synthesis kit (Invitrogen#4368814), total RNAs (5μg/reaction) were reverse transcribed into single –strand cDNA exactly based on instruction of protocol provided in the kit. .. Primers The genomic information of L. major was obtained from the following site: http//:Tritryp.org and the primers were designed according to sequence of AQP, TDR-1, and γ-GCS genes by Primer3 software.

    Polymerase Chain Reaction:

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: Paragraph title: Quantitative Reverse Transcriptase- PCR (qRT-PCR) – in vivo samples ... Samples were resuspended in 60μL water and 2 μg RNA from each sample were subsequently subjected to qRT-PCR using TaqMan RT-PCR ABI7900HT, in a two-step procedure, as following: 1) For reverse transcriptase step we utilized ABI High Capacity cDNA Reverse Transcription Kit (4368814,from Applied Biosystems).

    Article Title: Low-Molecular-Weight Heparin and Unfractionated Heparin Decrease Th-1, 2, and 17 Expressions
    Article Snippet: Total RNA(5ug) was reverse-transcribed using High-Capacity cDNA Reverse Transcription kits (Cat. 4368814)(Applied Biosystems) and random primers (Applied Biosystems). .. Quantitative PCR was performed by StepOne(tm) Real-Time PCR System (Applied Biosystems) with SYBR Green PCR Master Mix (Roche), and 1 ug cDNA was added to a volume of 20 uL.

    Article Title: Haloperidol induces pharmacoepigenetic response by modulating miRNA expression, global DNA methylation and expression profiles of methylation maintenance genes and genes involved in neurotransmission in neuronal cells
    Article Snippet: The total RNA was quantified using Nanodrop ND-1000 spectrophotometer and its quality was assessed by running in 1.2% agarose gel.1μg of total RNA was used for cDNA synthesis using High Capacity Reverse Transcriptase kit (Applied Biosystems Part No:4368814) as per the manufacturer’s instruction. .. In addition to these epigenetic genes we also profiled a panel of Human Neurotransmitter Receptors and Regulators (PAHS-060A-2) and Human Neuroscience Ion Channels & Transporters(PAHS-036A-2) using a 96-well PCR-array platform of RT2 Profiler PCR Arrays procured from Qiagen.

    Article Title: Deregulation of miR-34a and Its Chaperon Hsp70 in Hepatitis C virus-Induced Liver Cirrhosis and Hepatocellular Carcinoma Patients
    Article Snippet: Target gene heat shock protein-70 expression analysis High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, P/N 4368814) was used for RT reaction. .. Expression of Hsp70 (HSPA1A and HSPA1B) was quantified using the TaqMan® assay (Applied Biosystems, assay ID Hs00271244_s1), TaqMan® Endogenous control assay for Glyceraldhyde 3 Phosphate dehydrogenase (GAPDH) (Applied Biosystems, catalogue no 402869), and Taqman® Universal PCR master mix II, No UNG (2×) (Applied Biosystems, P/N 4440043).

    Nucleic Acid Electrophoresis:

    Article Title: MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells
    Article Snippet: .. RNA isolation and quantitative-PCR for miR-101 & target gene expression RNA was isolated using TRIzol (Sigma) method and the quality and quantity verified by formaldehyde gel electrophoresis and Nanodrop (ND-1000, Nanodrop), respectively. cDNA preparation was carried out, using the High-Throughput cDNA Preparation kit (Part No-4368813, Life Technologies), following manufacturer’s protocol, using 5 ng of RNA for cDNA synthesis of microRNA. .. TaqManRNU44 and has-miR-101 assays were performed by real time PCR.

    Article Title: Pumpkin (Cucurbita ficifolia Bouché) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression
    Article Snippet: RNA quantification was performed using NanoDrop 2000 (Thermo Scientific, Wilmington, DE, USA) and the quality and integrity of isolated RNA was confirmed using gel electrophoresis. .. 2 μg of total RNA was reverse transcribed using a high capacity cDNA reverse transcript kit [Part No: 4368814; Applied Biosystem (ABI)] according to the manufacturer’s protocol.

    In Vivo:

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: Paragraph title: Quantitative Reverse Transcriptase- PCR (qRT-PCR) – in vivo samples ... Samples were resuspended in 60μL water and 2 μg RNA from each sample were subsequently subjected to qRT-PCR using TaqMan RT-PCR ABI7900HT, in a two-step procedure, as following: 1) For reverse transcriptase step we utilized ABI High Capacity cDNA Reverse Transcription Kit (4368814,from Applied Biosystems).

    Isolation:

    Article Title: MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells
    Article Snippet: .. RNA isolation and quantitative-PCR for miR-101 & target gene expression RNA was isolated using TRIzol (Sigma) method and the quality and quantity verified by formaldehyde gel electrophoresis and Nanodrop (ND-1000, Nanodrop), respectively. cDNA preparation was carried out, using the High-Throughput cDNA Preparation kit (Part No-4368813, Life Technologies), following manufacturer’s protocol, using 5 ng of RNA for cDNA synthesis of microRNA. .. TaqManRNU44 and has-miR-101 assays were performed by real time PCR.

    Article Title: Association between EZH2 expression, silencing of tumor suppressors and disease outcome in solid tumors
    Article Snippet: .. RT-qPCR Total RNA was isolated using TRIzol reagent (Invitrogen). cDNA was synthetized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: Pumpkin (Cucurbita ficifolia Bouché) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression
    Article Snippet: Paragraph title: 2.5. RNA isolation and qRT-PCR gene expression analysis ... 2 μg of total RNA was reverse transcribed using a high capacity cDNA reverse transcript kit [Part No: 4368814; Applied Biosystem (ABI)] according to the manufacturer’s protocol.

    Article Title: BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation
    Article Snippet: .. RT-qPCR Total RNA was isolated using Trizol-Chloroform extraction and isopropanol precipitation. cDNA was synthesized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells
    Article Snippet: .. RT-qPCR from mouse tissues The total RNA was isolated using the Rneasy Mini Kit (Qiagen). cDNA was synthetized using High Capacity cDNA RT kit (4368814-Applied Biosystems), and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: Renal compartment-specific genetic variation analyses identify new pathways in chronic kidney disease
    Article Snippet: Quantitative Real-Time PCR RNA was isolated using RNeasy Mini Kit per manufacturer’s instructions. .. 1μg RNA was reverse transcribed using cDNA archival kit (Applied Biosystems#4368813), and quantitative real-time PCR was conducted in the ViiA 7 System (Applied Biosystems) machine using SYBRGreen Master Mix (Applied Biosystems#A25742).

    Purification:

    Article Title: Pumpkin (Cucurbita ficifolia Bouché) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression
    Article Snippet: RNA was isolated from control and different experimental adipocytes using the Total Tissue RNA purification kit (Norgen-Biotek Corp., Thorold, ON, Canada). .. 2 μg of total RNA was reverse transcribed using a high capacity cDNA reverse transcript kit [Part No: 4368814; Applied Biosystem (ABI)] according to the manufacturer’s protocol.

    Sequencing:

    Article Title: MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells
    Article Snippet: RNA isolation and quantitative-PCR for miR-101 & target gene expression RNA was isolated using TRIzol (Sigma) method and the quality and quantity verified by formaldehyde gel electrophoresis and Nanodrop (ND-1000, Nanodrop), respectively. cDNA preparation was carried out, using the High-Throughput cDNA Preparation kit (Part No-4368813, Life Technologies), following manufacturer’s protocol, using 5 ng of RNA for cDNA synthesis of microRNA. .. Target (miR-101) and reference (RNU44) genes were amplified using ABI PRISM 7000 sequence detection system (Applied Biosystems).

    Article Title: Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress; Use of APE1 small molecule inhibitors to delineate APE1 functions
    Article Snippet: The extracted RNA was quantified by a SmartSpec spectrophotometer (Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 260 nm. cDNA was synthesized by High-Capacity cDNA Reverse Transcription Kit according to the protocol provided by the manufacture (Part No.: 4368814 Applied Biosystems). .. The primers for TNF-α were 5’-AGGCTGCCCCGACTATGTG-3’ and 5’-AGGAGGCTGACTTTCTCCTGGTA-3’; the primers for GAPDH were 5’-GTATGACTCTAC CCACGGCAAGT-3’ and 5’-TCTCGCTCCTGGAAGATGGT-3’. qRT-PCR was conducted on an ABI PRISM 7500 Sequence Detection System (Applied Biosystems) using RT2-SYBR Green /ROX qPCR Master Mix (SABiosciences, Frederick, MD) in a 25ul final reaction mixture.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Dose-dependent effects of small-molecule antagonists on the genomic landscape of androgen receptor binding
    Article Snippet: Homogenates were then processed using Qiagen RNeasy Plus kit (Cat74134). .. Samples were resuspended in 60μL water and 2 μg RNA from each sample were subsequently subjected to qRT-PCR using TaqMan RT-PCR ABI7900HT, in a two-step procedure, as following: 1) For reverse transcriptase step we utilized ABI High Capacity cDNA Reverse Transcription Kit (4368814,from Applied Biosystems).

    TaqMan Assay:

    Article Title: Deregulation of miR-34a and Its Chaperon Hsp70 in Hepatitis C virus-Induced Liver Cirrhosis and Hepatocellular Carcinoma Patients
    Article Snippet: Target gene heat shock protein-70 expression analysis High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, P/N 4368814) was used for RT reaction. .. Expression of Hsp70 (HSPA1A and HSPA1B) was quantified using the TaqMan® assay (Applied Biosystems, assay ID Hs00271244_s1), TaqMan® Endogenous control assay for Glyceraldhyde 3 Phosphate dehydrogenase (GAPDH) (Applied Biosystems, catalogue no 402869), and Taqman® Universal PCR master mix II, No UNG (2×) (Applied Biosystems, P/N 4440043).

    SYBR Green Assay:

    Article Title: MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells
    Article Snippet: RNA isolation and quantitative-PCR for miR-101 & target gene expression RNA was isolated using TRIzol (Sigma) method and the quality and quantity verified by formaldehyde gel electrophoresis and Nanodrop (ND-1000, Nanodrop), respectively. cDNA preparation was carried out, using the High-Throughput cDNA Preparation kit (Part No-4368813, Life Technologies), following manufacturer’s protocol, using 5 ng of RNA for cDNA synthesis of microRNA. .. SYBR green Real-Time PCR was performed using SYBR Green reaction mix and UBE2N & SMARCA4 specific primers, designed using both online and offline tools, i.e. primer 3 and Oligo, respectively.

    Article Title: Association between EZH2 expression, silencing of tumor suppressors and disease outcome in solid tumors
    Article Snippet: .. RT-qPCR Total RNA was isolated using TRIzol reagent (Invitrogen). cDNA was synthetized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation
    Article Snippet: .. RT-qPCR Total RNA was isolated using Trizol-Chloroform extraction and isopropanol precipitation. cDNA was synthesized using High Capacity cDNA RT kit (4368814-Applied Biosystems) and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells
    Article Snippet: .. RT-qPCR from mouse tissues The total RNA was isolated using the Rneasy Mini Kit (Qiagen). cDNA was synthetized using High Capacity cDNA RT kit (4368814-Applied Biosystems), and quantitative PCR was performed with technical triplicate using SYBR green reagent (Roche) on a ViiA7 equipment (Applied Biosystems). ..

    Article Title: Low-Molecular-Weight Heparin and Unfractionated Heparin Decrease Th-1, 2, and 17 Expressions
    Article Snippet: Total RNA(5ug) was reverse-transcribed using High-Capacity cDNA Reverse Transcription kits (Cat. 4368814)(Applied Biosystems) and random primers (Applied Biosystems). .. Quantitative PCR was performed by StepOne(tm) Real-Time PCR System (Applied Biosystems) with SYBR Green PCR Master Mix (Roche), and 1 ug cDNA was added to a volume of 20 uL.

    Article Title: Haloperidol induces pharmacoepigenetic response by modulating miRNA expression, global DNA methylation and expression profiles of methylation maintenance genes and genes involved in neurotransmission in neuronal cells
    Article Snippet: The total RNA was quantified using Nanodrop ND-1000 spectrophotometer and its quality was assessed by running in 1.2% agarose gel.1μg of total RNA was used for cDNA synthesis using High Capacity Reverse Transcriptase kit (Applied Biosystems Part No:4368814) as per the manufacturer’s instruction. .. The expression of epigenetic genes (DNMT1 , DNMT3A , DNMT3B ,MBD2 ) were analysed using real time quantification of cDNA using either Taqman gene expression assay (Applied Biosystems) or SYBR Green based assays (Applied Biosystems). ß-actin (ACTB) was used as the endogenous control.

    RNA Extraction:

    Article Title: Haloperidol induces pharmacoepigenetic response by modulating miRNA expression, global DNA methylation and expression profiles of methylation maintenance genes and genes involved in neurotransmission in neuronal cells
    Article Snippet: Paragraph title: RNA extraction, reverse transcription and real-time PCR analysis ... The total RNA was quantified using Nanodrop ND-1000 spectrophotometer and its quality was assessed by running in 1.2% agarose gel.1μg of total RNA was used for cDNA synthesis using High Capacity Reverse Transcriptase kit (Applied Biosystems Part No:4368814) as per the manufacturer’s instruction.

    Sample Prep:

    Article Title: Genome-Wide Bovine H3K27me3 Modifications and the Regulatory Effects on Genes Expressions in Peripheral Blood Lymphocytes
    Article Snippet: Approximately 6 µg of total RNA was transcribed into double-stranded cDNA through Reverse Transcription Kit (Applied Biosystems, PN.4368814). .. Sample preparation for digital gene expression profiles was achieved through the Gene Expression Sample Prep Kit (Illumina, Part #1004241) with details as follows.

    TLDA Assay:

    Article Title: Pumpkin (Cucurbita ficifolia Bouché) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression
    Article Snippet: 2 μg of total RNA was reverse transcribed using a high capacity cDNA reverse transcript kit [Part No: 4368814; Applied Biosystem (ABI)] according to the manufacturer’s protocol. .. The expression levels of adipogenic-related genes [ADIPOQ, FABP 4, PPARGC1A, ACACB, CEBPB, CEBPA, and LPL] were quantified using custom Taqman low density array cards (S1 Table), and ViiA 7 real-time PCR device (ABI).

    Spectrophotometry:

    Article Title: Haloperidol induces pharmacoepigenetic response by modulating miRNA expression, global DNA methylation and expression profiles of methylation maintenance genes and genes involved in neurotransmission in neuronal cells
    Article Snippet: .. The total RNA was quantified using Nanodrop ND-1000 spectrophotometer and its quality was assessed by running in 1.2% agarose gel.1μg of total RNA was used for cDNA synthesis using High Capacity Reverse Transcriptase kit (Applied Biosystems Part No:4368814) as per the manufacturer’s instruction. .. The expression of epigenetic genes (DNMT1 , DNMT3A , DNMT3B ,MBD2 ) were analysed using real time quantification of cDNA using either Taqman gene expression assay (Applied Biosystems) or SYBR Green based assays (Applied Biosystems). ß-actin (ACTB) was used as the endogenous control.

    Article Title: Role of APE1 in differentiated neuroblastoma SH-SY5Y cells in response to oxidative stress; Use of APE1 small molecule inhibitors to delineate APE1 functions
    Article Snippet: .. The extracted RNA was quantified by a SmartSpec spectrophotometer (Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 260 nm. cDNA was synthesized by High-Capacity cDNA Reverse Transcription Kit according to the protocol provided by the manufacture (Part No.: 4368814 Applied Biosystems). .. The primers for TNF-α were 5’-AGGCTGCCCCGACTATGTG-3’ and 5’-AGGAGGCTGACTTTCTCCTGGTA-3’; the primers for GAPDH were 5’-GTATGACTCTAC CCACGGCAAGT-3’ and 5’-TCTCGCTCCTGGAAGATGGT-3’. qRT-PCR was conducted on an ABI PRISM 7500 Sequence Detection System (Applied Biosystems) using RT2-SYBR Green /ROX qPCR Master Mix (SABiosciences, Frederick, MD) in a 25ul final reaction mixture.

    High Throughput Screening Assay:

    Article Title: MiR-101 Induces Senescence and Prevents Apoptosis in the Background of DNA Damage in MCF7 Cells
    Article Snippet: .. RNA isolation and quantitative-PCR for miR-101 & target gene expression RNA was isolated using TRIzol (Sigma) method and the quality and quantity verified by formaldehyde gel electrophoresis and Nanodrop (ND-1000, Nanodrop), respectively. cDNA preparation was carried out, using the High-Throughput cDNA Preparation kit (Part No-4368813, Life Technologies), following manufacturer’s protocol, using 5 ng of RNA for cDNA synthesis of microRNA. .. TaqManRNU44 and has-miR-101 assays were performed by real time PCR.

    Control Assay:

    Article Title: Deregulation of miR-34a and Its Chaperon Hsp70 in Hepatitis C virus-Induced Liver Cirrhosis and Hepatocellular Carcinoma Patients
    Article Snippet: Target gene heat shock protein-70 expression analysis High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, P/N 4368814) was used for RT reaction. .. Expression of Hsp70 (HSPA1A and HSPA1B) was quantified using the TaqMan® assay (Applied Biosystems, assay ID Hs00271244_s1), TaqMan® Endogenous control assay for Glyceraldhyde 3 Phosphate dehydrogenase (GAPDH) (Applied Biosystems, catalogue no 402869), and Taqman® Universal PCR master mix II, No UNG (2×) (Applied Biosystems, P/N 4440043).

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    Thermo Fisher tri reagent
    Tri Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high capacity cdna reverse transcription kit
    High Capacity Cdna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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