Journal: Journal of Translational Medicine
Article Title: Novel insights into hypoxia-driven transcriptomic and epigenetic landscapes in grade 3 meningioma
doi: 10.1186/s12967-025-07606-9
Figure Lengend Snippet: Transcription Factor Enrichment and HRE Analysis in DEGs. ( A ) Transcription factor enrichment in upregulated genes. ( B ) Transcription factor enrichment in downregulated genes. ( C ) Illustration showing the position of hypoxia response elements (HREs) within the promoters of hypoxia-induced genes. ( D ) RT-qPCR validation of genes containing HREs upon HIF-1A overexpression. (* p < 0.05; ** p < 0.01; *** p < 0.001)
Article Snippet: Membranes were blocked with 5% BSA for 1 h at room temperature with gentle agitation, followed by overnight incubation at 4 °C with primary antibodies (1:1000 dilution of IGFBP3 antibody (Cell Signaling Technology #64143), 1:1000 dilution of NDRG1 antibody (Cell Signaling Technology #5196), 1:1000 dilution of HIF-1A antibody (Cell Signaling Technology #36169).
Techniques: Quantitative RT-PCR, Biomarker Discovery, Over Expression