hi scribe t7 transcription kit  (New England Biolabs)


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    Structured Review

    New England Biolabs hi scribe t7 transcription kit
    Hi Scribe T7 Transcription Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hi scribe t7 transcription kit/product/New England Biolabs
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hi scribe t7 transcription kit - by Bioz Stars, 2019-10
    84/100 stars

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    Transfection:

    Article Title: Promoter-proximal pausing mediated by the exon junction complex regulates splicing
    Article Snippet: The plasmids expressing various transgenes were transfected with Effectene transfection reagent (Qiagen, Cat No-301425), following manufacturer’s protocol. .. For knock down experiments, dsRNA was synthesized overnight at 37 °C using Hi-Scribe T7 transcription kit (NEB, Cat No-E2040). dsRNA was transfected in S2R+ cells by serum starvation for 6 h. The treatment was repeated three times and cells were harvested 7 days after the first treatment for Mago. .. For knockdown of other pre-EJC components and Btz, the treatment was repeated two times and cells were harvested 5 days after the first treatment.

    Cell Culture:

    Article Title: Promoter-proximal pausing mediated by the exon junction complex regulates splicing
    Article Snippet: Paragraph title: Cell culture, RNAi, and transfection ... For knock down experiments, dsRNA was synthesized overnight at 37 °C using Hi-Scribe T7 transcription kit (NEB, Cat No-E2040). dsRNA was transfected in S2R+ cells by serum starvation for 6 h. The treatment was repeated three times and cells were harvested 7 days after the first treatment for Mago.

    Expressing:

    Article Title: Promoter-proximal pausing mediated by the exon junction complex regulates splicing
    Article Snippet: The plasmids expressing various transgenes were transfected with Effectene transfection reagent (Qiagen, Cat No-301425), following manufacturer’s protocol. .. For knock down experiments, dsRNA was synthesized overnight at 37 °C using Hi-Scribe T7 transcription kit (NEB, Cat No-E2040). dsRNA was transfected in S2R+ cells by serum starvation for 6 h. The treatment was repeated three times and cells were harvested 7 days after the first treatment for Mago.

    Blocking Assay:

    Article Title: Promoter-proximal pausing mediated by the exon junction complex regulates splicing
    Article Snippet: For knock down experiments, dsRNA was synthesized overnight at 37 °C using Hi-Scribe T7 transcription kit (NEB, Cat No-E2040). dsRNA was transfected in S2R+ cells by serum starvation for 6 h. The treatment was repeated three times and cells were harvested 7 days after the first treatment for Mago. .. For knock down experiments, dsRNA was synthesized overnight at 37 °C using Hi-Scribe T7 transcription kit (NEB, Cat No-E2040). dsRNA was transfected in S2R+ cells by serum starvation for 6 h. The treatment was repeated three times and cells were harvested 7 days after the first treatment for Mago.

    Synthesized:

    Article Title: Promoter-proximal pausing mediated by the exon junction complex regulates splicing
    Article Snippet: The plasmids expressing various transgenes were transfected with Effectene transfection reagent (Qiagen, Cat No-301425), following manufacturer’s protocol. .. For knock down experiments, dsRNA was synthesized overnight at 37 °C using Hi-Scribe T7 transcription kit (NEB, Cat No-E2040). dsRNA was transfected in S2R+ cells by serum starvation for 6 h. The treatment was repeated three times and cells were harvested 7 days after the first treatment for Mago. .. For knockdown of other pre-EJC components and Btz, the treatment was repeated two times and cells were harvested 5 days after the first treatment.

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    New England Biolabs hi scribe rna transcription kit
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    New England Biolabs hi scribe t7 transcription kit
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    https://www.bioz.com/result/hi scribe t7 transcription kit/product/New England Biolabs
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