hi fidelity extensor long range pcr enzyme kit  (Thermo Fisher)


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    Extensor Long Range PCR Polymerase Blend Kits and Master Mixes
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    Catalog Number:
    ab-0792/b
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    Structured Review

    Thermo Fisher hi fidelity extensor long range pcr enzyme kit

    https://www.bioz.com/result/hi fidelity extensor long range pcr enzyme kit/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    hi fidelity extensor long range pcr enzyme kit - by Bioz Stars, 2021-01
    99/100 stars

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    Polymerase Chain Reaction:

    Article Title: In Silico Studies of Outer Membrane of Neisseria Meningitidis Por A: Its Expression and Immunogenic Properties
    Article Snippet: .. Amplification of porA The porA gene was amplified from N. meningitidis chromosomal DNA by the polymerase chain reaction (PCR) with a PCR kit from Fermentas in accordance with the manufacturer’s instruction. .. The primers were designed on the basis of the published nucleotide sequence of porA from N. meningitidis .

    Article Title: Testing whether macroevolution follows microevolution: Are colour differences among swans (Cygnus) attributable to variation at the MC1R locus?
    Article Snippet: .. A 1.3 kb segment of MC1R including the entire coding region was amplified using a forward primer designed to the 5' UTR of the chicken MC1R gene (Chick 5' utr; 5'-GAGAAAGGGCCCTTTCTTC-3') and a reverse primer designed to the isolated 3' UTR of the C. atratus MC1R gene (Swan 3' utr; 5'-TAGCCTTTATTCGGTACCG-3') in a PCR containing 2.5U taq (Extensor Hi-Fidelity PCR Master Mix, Abgene), 2.25 mM MgCl2 , 350 μM dNTPs, 0.2 μM of each primer and 50 ng DNA with cycling conditions of 94°C 3 min, 40 × 94°C 30 s, 62°C 45 s, 70°C 45 s; 70°C 5 min. For some samples, a new 5' UTR primer (Swan 5' utr; 5'-GCCCATGTCCTCTTGACC-3') was designed that bound downstream of the chicken 5' primer. .. PCR products were sequenced on both strands with BigDye v. 3.1 (Applied Biosystems) using PCR primers and the internal primers MSHR73 (5'-GGCGTAGAAGATGGTGATGTAGC-3') and MSHR74 (5'-GTGGACCGCTACATCACCAT-3').

    Article Title: A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes
    Article Snippet: .. The PCR was performed using the Thermo Scientific Hi-Fidelity Extensor Long-Range PCR Enzyme kit with 9 μl of the 2 × mix and 14 μl of water per reaction (Cat. No. AB-0792/A and AB-0720/B). .. A touchdown method was used for the PCR cycling conditions to account for differing melting temperatures of the primers.

    Article Title: Quantitative Proteomic and Network Analysis of Differentially Expressed Proteins in PBMC of Friedreich’s Ataxia (FRDA) Patients
    Article Snippet: .. Genetic Analysis for (GAA) Repeats Long-range PCR Kit containing LR-PCR buffer, dNTPs, and long-range Taq polymerase and a set of primers were obtained from Thermo Fisher Scientific, United States. .. The PCR amplification for all the samples was done twice on an MJ Mini Thermocycler (Bio-Rad Laboratories, United States).

    Article Title: Molecular approaches underlying the oogenic cycle of the scleractinian coral, Acropora tenuis
    Article Snippet: .. Partial fragments of AtVasa and AtLDLR were amplified by long-range polymerase chain reaction (PCR) using the following conditions: LA Taq DNA Polymerase, 35 cycles of denaturation (10 s at 98 °C), annealing (30 s at 60 °C), and extension (2 min at 68 °C). .. PCR products were subcloned into the pGEM-T Easy vector (Promega, Madison, WI, USA) and transformed into JM109 competent cells (Takara Bio).

    Article Title: RAF gene fusion breakpoints in pediatric brain tumors are characterized by significant enrichment of sequence microhomology
    Article Snippet: .. For those tumors where the breakpoints failed to amplify, we repeated the aforementioned assay using the Extensor Long Range PCR Kit (Thermo Fisher Scientific) instead of Platinum Taq DNA polymerase (Invitrogen). ..

    Article Title: An Extensive Invasive Intracranial Human Glioblastoma Xenograft Model
    Article Snippet: .. A PCR kit was obtained from Invitrogen Corp. (Carlsbad, CA). .. The mouse anti-human Ki-67 antibody was from Dako (Carpinteria, CA), and the mouse anti-human vimentin antibody was from Biogenex Laboratory Inc (San Ramon, CA).

    Isolation:

    Article Title: Testing whether macroevolution follows microevolution: Are colour differences among swans (Cygnus) attributable to variation at the MC1R locus?
    Article Snippet: .. A 1.3 kb segment of MC1R including the entire coding region was amplified using a forward primer designed to the 5' UTR of the chicken MC1R gene (Chick 5' utr; 5'-GAGAAAGGGCCCTTTCTTC-3') and a reverse primer designed to the isolated 3' UTR of the C. atratus MC1R gene (Swan 3' utr; 5'-TAGCCTTTATTCGGTACCG-3') in a PCR containing 2.5U taq (Extensor Hi-Fidelity PCR Master Mix, Abgene), 2.25 mM MgCl2 , 350 μM dNTPs, 0.2 μM of each primer and 50 ng DNA with cycling conditions of 94°C 3 min, 40 × 94°C 30 s, 62°C 45 s, 70°C 45 s; 70°C 5 min. For some samples, a new 5' UTR primer (Swan 5' utr; 5'-GCCCATGTCCTCTTGACC-3') was designed that bound downstream of the chicken 5' primer. .. PCR products were sequenced on both strands with BigDye v. 3.1 (Applied Biosystems) using PCR primers and the internal primers MSHR73 (5'-GGCGTAGAAGATGGTGATGTAGC-3') and MSHR74 (5'-GTGGACCGCTACATCACCAT-3').

    Amplification:

    Article Title: In Silico Studies of Outer Membrane of Neisseria Meningitidis Por A: Its Expression and Immunogenic Properties
    Article Snippet: .. Amplification of porA The porA gene was amplified from N. meningitidis chromosomal DNA by the polymerase chain reaction (PCR) with a PCR kit from Fermentas in accordance with the manufacturer’s instruction. .. The primers were designed on the basis of the published nucleotide sequence of porA from N. meningitidis .

    Article Title: Testing whether macroevolution follows microevolution: Are colour differences among swans (Cygnus) attributable to variation at the MC1R locus?
    Article Snippet: .. A 1.3 kb segment of MC1R including the entire coding region was amplified using a forward primer designed to the 5' UTR of the chicken MC1R gene (Chick 5' utr; 5'-GAGAAAGGGCCCTTTCTTC-3') and a reverse primer designed to the isolated 3' UTR of the C. atratus MC1R gene (Swan 3' utr; 5'-TAGCCTTTATTCGGTACCG-3') in a PCR containing 2.5U taq (Extensor Hi-Fidelity PCR Master Mix, Abgene), 2.25 mM MgCl2 , 350 μM dNTPs, 0.2 μM of each primer and 50 ng DNA with cycling conditions of 94°C 3 min, 40 × 94°C 30 s, 62°C 45 s, 70°C 45 s; 70°C 5 min. For some samples, a new 5' UTR primer (Swan 5' utr; 5'-GCCCATGTCCTCTTGACC-3') was designed that bound downstream of the chicken 5' primer. .. PCR products were sequenced on both strands with BigDye v. 3.1 (Applied Biosystems) using PCR primers and the internal primers MSHR73 (5'-GGCGTAGAAGATGGTGATGTAGC-3') and MSHR74 (5'-GTGGACCGCTACATCACCAT-3').

    Article Title: Molecular approaches underlying the oogenic cycle of the scleractinian coral, Acropora tenuis
    Article Snippet: .. Partial fragments of AtVasa and AtLDLR were amplified by long-range polymerase chain reaction (PCR) using the following conditions: LA Taq DNA Polymerase, 35 cycles of denaturation (10 s at 98 °C), annealing (30 s at 60 °C), and extension (2 min at 68 °C). .. PCR products were subcloned into the pGEM-T Easy vector (Promega, Madison, WI, USA) and transformed into JM109 competent cells (Takara Bio).

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  • 99
    Thermo Fisher hi fidelity extensor long range pcr enzyme kit
    Hi Fidelity Extensor Long Range Pcr Enzyme Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hi fidelity extensor long range pcr enzyme kit/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    hi fidelity extensor long range pcr enzyme kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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