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Flow Laboratories hepes
Hepes, supplied by Flow Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hepes/product/Flow Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hepes - by Bioz Stars, 2020-04
86/100 stars

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Flow Cytometry:

Article Title: Influence of Mycobacterium bovis Bacillus Calmette Gu?rin on In Vitro Induction of CD1 Molecules in Human Adherent Mononuclear Cells
Article Snippet: .. Lymphoblastoid cells were grown in suspension in RPMI 1640 medium (GIBCO, Paisley, Scotland) supplemented with 10% fetal calf serum (GIBCO), 10 mM HEPES (Flow Laboratories, McLean, Va.), and 2 mM l -glutamine (Flow) and subcultured two or three times weekly. .. Peripheral blood mononuclear cells were separated from heparinized whole blood obtained from healthy donors on a Ficoll-Hypaque (Pharmacia, Uppsala, Sweden) gradient, washed twice in RPMI 1640 medium, and resuspended in RPMI 1640 medium supplemented with 10% fetal calf serum, 10 mM HEPES, 2 mM l -glutamine, 0.8 mM nonessential amino acids (GIBCO), 0.4 mM essential amino acids, and 50 μM 2-mercaptoethanol (hereafter referred to as complete medium [CM]).

Article Title: Rifampin Increases Cytokine-Induced Expression of the CD1b Molecule in Human Peripheral Blood Monocytes
Article Snippet: .. Peripheral blood mononuclear cells were separated from heparinized whole blood, obtained from healthy donors, on a Ficoll-Hypaque (Pharmacia, Uppsala, Sweden) gradient, washed twice in RPMI 1640 medium (HyClone Europe Ltd., Cramlington, United Kingdom), and resuspended in RPMI 1640 medium supplemented with 10% fetal calf serum (HyClone), 10 mM HEPES (Flow Laboratories, McLean, Va.), 2 mM l -glutamine (Flow), 0.8 mM nonessential amino acids (GIBCO, Paisley, Scotland), 0.4 mM essential amino acids, and 50 μM 2-mercaptoethanol (Sigma) (hereafter referred to as complete medium). ..

Article Title: Activity of Monosaccharide Lipid A Analogues in Human Monocytic Cells as Agonists or Antagonists of Bacterial Lipopolysaccharide
Article Snippet: For culture of cells, RPMI 1640 medium (Flow Laboratories, Inc., Rockville, Md.) supplemented with 10 mM HEPES, 2 mM l -glutamine, 100 U of penicillin per ml, 100 μg of streptomycin per ml, and 0.2% NaHCO3 was used as the basic medium and heat-inactivated fetal calf serum (FCS; Flow Laboratories) was added at a concentration of 5 or 10% (5 or 10% FCS-RPMI medium). .. These cell suspensions were dispensed (0.5 ml) to each well of a 48-well culture plate (Sumitomo Bakelite Co. Ltd., Tokyo, Japan) and cultured for 2 h. The cells in each well were washed three times with 0.5 ml of Hanks' balanced salt solution (Flow Laboratories), and adherent cells were cultured with 5% FCS-RPMI medium in the presence of test samples (0.5 ml/well).

Article Title: ?2-Microglobulin is Required for the Full Expression of Xenobiotic-Induced Systemic Autoimmunity
Article Snippet: Paragraph title: Flow Cytometry ... Bioproducts, Walkersville, MD) containing 10% FCS (Hyclone Laboratories, Logan, UT), 2 mM L-glutamine (Sigma Chemical Co., St. Louis, MO), 10 mM HEPES (pH 7.2; Flow Laboratories, Irvine, Scotland), 1% non-essential amino acids (Irvine Scientific, Santa Ana, CA), 20 mM sodium carbonate (Fisher Scientific Company, Fair Lawn, NJ), and 20 ug/ml gentamicin sulfate (Irvine Scientific).

Article Title: Modification of the Structure and Activity of Lipid A in Yersinia pestis Lipopolysaccharide by Growth Temperature
Article Snippet: For culture of cells, RPMI 1640 medium (Flow Laboratories, Inc., Rockville, Md.) supplemented with 10 mM HEPES, 2 mM l -glutamine, 100 U of penicillin per ml, 100 μg of streptomycin per ml, and 0.2% NaHCO3 was used as the basic medium, and heat-inactivated fetal calf serum (FCS; Flow Laboratories) was added at a concentration of 5 or 10% (5 or 10% FCS-RPMI medium). .. These cell suspensions were dispensed (0.5 ml) to each well of a 48-well culture plate and cultured for 2 h. The cells in each well were washed three times with 0.5 ml of Hanks' balanced salt solution (Flow Laboratories), and adherent cells were cultured with 5% FCS-RPMI medium in the presence of test samples (0.5 ml/well).

Stable Transfection:

Article Title: Influence of Mycobacterium bovis Bacillus Calmette Gu?rin on In Vitro Induction of CD1 Molecules in Human Adherent Mononuclear Cells
Article Snippet: The CD1b-transfected C1R.b6 lymphoblastoid cell line was generated by stably transfecting human lymphoblastoid C1R cells with pSRα-NEO vector containing CD1b cDNA, as previously described ( ). .. Lymphoblastoid cells were grown in suspension in RPMI 1640 medium (GIBCO, Paisley, Scotland) supplemented with 10% fetal calf serum (GIBCO), 10 mM HEPES (Flow Laboratories, McLean, Va.), and 2 mM l -glutamine (Flow) and subcultured two or three times weekly.

Multiple Displacement Amplification:

Article Title: Synergistic inhibition of human cancer cell growth by cytotoxic drugs and mixed backbone antisense oligonucleotide targeting protein kinase A
Article Snippet: LS 174T, MDA-MB-231, and MDA-MB-468 cell lines were maintained in DMEM. .. All media, purchased from Flow Laboratories, were supplemented with 10% heat inactivated fetal bovine serum/20 mM Hepes, pH 7.4/5 mM glutamine/100 units ml penicillin/100 μg/ml streptomycin (Flow Laboratories).

Construct:

Article Title: Effect of Dirithromycin on Haemophilus influenzae Infection of the Respiratory Mucosa
Article Snippet: The strips were dispersed by gentle agitation of the brush in 2 ml of cell culture medium 199 with Earle’s salts and HEPES ( N -hydroxyethylpiperazine- N ′-2-ethanesulfonic acid; Flow Laboratories, Irvine, Scotland, United Kingdom). .. For each experiment ( n = 6), three sealed microscope coverslip-slide preparations containing approximately equal numbers of strips of nasal epithelium were constructed: medium 199 alone (control), SH9 culture filtrate (1:1) with epithelium in medium 199, and SH9 culture filtrate (1:1) with epithelium preincubated with dirithromycin in medium 199.

Growth Inhibition Assay:

Article Title: Activation of Rat Alveolar Macrophage-Derived Latent Transforming Growth Factor ?-1 by Plasmin Requires Interaction with Thrombospondin-1 and its Cell Surface Receptor, CD36
Article Snippet: Paragraph title: CCL-64 Mink Lung Epithelial Growth Inhibition Assay for TGF-β ... Briefly, to subconfluent cells in 0.2% BCP and resuspended in α-MEM, 0.2% BCP, 10 mmol/L Hepes at pH 7.4, penicillin (25 μg/ml) and streptomycin (25 μg/ml), and cultured as 5 × 10 cells per 0.5 ml in 24-well costar dishes (Flow Laboratories, Inc., Mississauga, ON) was added neutral CM or CM that was acidified and subsequently neutralized.

Plasmid Preparation:

Article Title: Influence of Mycobacterium bovis Bacillus Calmette Gu?rin on In Vitro Induction of CD1 Molecules in Human Adherent Mononuclear Cells
Article Snippet: The control, CD1b-negative C1R/MOCK subline was obtained following transfection of C1R cells with a mock plasmid. .. Lymphoblastoid cells were grown in suspension in RPMI 1640 medium (GIBCO, Paisley, Scotland) supplemented with 10% fetal calf serum (GIBCO), 10 mM HEPES (Flow Laboratories, McLean, Va.), and 2 mM l -glutamine (Flow) and subcultured two or three times weekly.

Transfection:

Article Title: Influence of Mycobacterium bovis Bacillus Calmette Gu?rin on In Vitro Induction of CD1 Molecules in Human Adherent Mononuclear Cells
Article Snippet: The control, CD1b-negative C1R/MOCK subline was obtained following transfection of C1R cells with a mock plasmid. .. Lymphoblastoid cells were grown in suspension in RPMI 1640 medium (GIBCO, Paisley, Scotland) supplemented with 10% fetal calf serum (GIBCO), 10 mM HEPES (Flow Laboratories, McLean, Va.), and 2 mM l -glutamine (Flow) and subcultured two or three times weekly.

Infection:

Article Title: Effect of Dirithromycin on Haemophilus influenzae Infection of the Respiratory Mucosa
Article Snippet: Strips of normal human nasal ciliated epithelium were obtained with a cytology brush ( , ) from both inferior turbinates of healthy volunteers who had been free of respiratory infection for at least 4 weeks. .. The strips were dispersed by gentle agitation of the brush in 2 ml of cell culture medium 199 with Earle’s salts and HEPES ( N -hydroxyethylpiperazine- N ′-2-ethanesulfonic acid; Flow Laboratories, Irvine, Scotland, United Kingdom).

Mouse Assay:

Article Title: NK Cells Mediate Increase of Phagocytic Activity but Not of Proinflammatory Cytokine (Interleukin-6 [IL-6], Tumor Necrosis Factor Alpha, and IL-12) Production Elicited in Splenic Macrophages by Tilorone Treatment of Mice during Acute Systemic Candidiasis
Article Snippet: At the end of the experiments, mice were anesthetized with ether and killed by cervical dislocation. .. Spleens were extracted under aseptic conditions, and cells were gently dispersed by using 90-mesh stainless steel screens into cold complete RPMI 1640 medium (Sigma) containing 1% penicillin G-streptomycin solution (Sigma), 1% l -glutamine (Sigma), 1% sodium pyruvate (Sigma), 1% HEPES (Flow Laboratories, Irvine, United Kingdom) and 5% fetal calf serum (FCS; Flow Laboratories).

Light Microscopy:

Article Title: Effect of Dirithromycin on Haemophilus influenzae Infection of the Respiratory Mucosa
Article Snippet: The strips were dispersed by gentle agitation of the brush in 2 ml of cell culture medium 199 with Earle’s salts and HEPES ( N -hydroxyethylpiperazine- N ′-2-ethanesulfonic acid; Flow Laboratories, Irvine, Scotland, United Kingdom). .. CBF was measured by a photometric technique , and epithelial integrity was assessed by light microscopy ( , ) at hourly intervals for 4 h. At each time point 10 recordings of CBF were made from the same sites on the epithelium, and each site was recorded as either intact (smooth outline of epithelium) or disrupted (outline uneven due to disruption of the integrity of the epithelium and cell projection).

Concentration Assay:

Article Title: Activity of Monosaccharide Lipid A Analogues in Human Monocytic Cells as Agonists or Antagonists of Bacterial Lipopolysaccharide
Article Snippet: .. For culture of cells, RPMI 1640 medium (Flow Laboratories, Inc., Rockville, Md.) supplemented with 10 mM HEPES, 2 mM l -glutamine, 100 U of penicillin per ml, 100 μg of streptomycin per ml, and 0.2% NaHCO3 was used as the basic medium and heat-inactivated fetal calf serum (FCS; Flow Laboratories) was added at a concentration of 5 or 10% (5 or 10% FCS-RPMI medium). ..

Article Title: Modification of the Structure and Activity of Lipid A in Yersinia pestis Lipopolysaccharide by Growth Temperature
Article Snippet: .. For culture of cells, RPMI 1640 medium (Flow Laboratories, Inc., Rockville, Md.) supplemented with 10 mM HEPES, 2 mM l -glutamine, 100 U of penicillin per ml, 100 μg of streptomycin per ml, and 0.2% NaHCO3 was used as the basic medium, and heat-inactivated fetal calf serum (FCS; Flow Laboratories) was added at a concentration of 5 or 10% (5 or 10% FCS-RPMI medium). ..

Incubation:

Article Title: Effect of Lactobacillus fermentum on Beta2 Toxin Production by Clostridium perfringens ▿
Article Snippet: Human-derived, enterocyte-like Caco-2 cells ( ) were seeded into 12-well plates (Greiner) at 80,000 cells/cm2 and cultured in Dulbecco's modified Eagle medium (DMEM; Invitrogen) supplemented with 0.03% l -glutamine (Invitrogen), 1% nonessential amino acids (Flow Laboratories), 10 mM sodium bicarbonate, 25 mM HEPES (Flow Laboratories), 50 μg/ml gentamicin (Gibco), and 20% fetal bovine serum (Lonza) in a humid atmosphere of 5% CO2 at 37°C. .. At day 5, the medium was removed and the monolayers were washed twice with 0.01 M phosphate-buffered saline (PBS), pH 7.3, and incubated with plain DMEM.

Article Title: ?2-Microglobulin is Required for the Full Expression of Xenobiotic-Induced Systemic Autoimmunity
Article Snippet: Bioproducts, Walkersville, MD) containing 10% FCS (Hyclone Laboratories, Logan, UT), 2 mM L-glutamine (Sigma Chemical Co., St. Louis, MO), 10 mM HEPES (pH 7.2; Flow Laboratories, Irvine, Scotland), 1% non-essential amino acids (Irvine Scientific, Santa Ana, CA), 20 mM sodium carbonate (Fisher Scientific Company, Fair Lawn, NJ), and 20 ug/ml gentamicin sulfate (Irvine Scientific). .. After washing in complete RPMI 1640 and then PBS containing 0.04% horse serum (Gemini Bio-Products, Woodland, CA), cells were incubated in PBS/0.04% horse serum with Fc Block and then stained with combinations of anti-NK1.1-FITC, Thy1-PE, anti-B220-PerCP, anti-CD3-APC, anti-CD4-FITC, or anti-CD8α-PerCP for 30 min at 4°C, or appropriate isotype controls.

Cell Culture:

Article Title: Activity of Monosaccharide Lipid A Analogues in Human Monocytic Cells as Agonists or Antagonists of Bacterial Lipopolysaccharide
Article Snippet: Paragraph title: Cell culture. ... For culture of cells, RPMI 1640 medium (Flow Laboratories, Inc., Rockville, Md.) supplemented with 10 mM HEPES, 2 mM l -glutamine, 100 U of penicillin per ml, 100 μg of streptomycin per ml, and 0.2% NaHCO3 was used as the basic medium and heat-inactivated fetal calf serum (FCS; Flow Laboratories) was added at a concentration of 5 or 10% (5 or 10% FCS-RPMI medium).

Article Title: Activation of Rat Alveolar Macrophage-Derived Latent Transforming Growth Factor ?-1 by Plasmin Requires Interaction with Thrombospondin-1 and its Cell Surface Receptor, CD36
Article Snippet: .. Briefly, to subconfluent cells in 0.2% BCP and resuspended in α-MEM, 0.2% BCP, 10 mmol/L Hepes at pH 7.4, penicillin (25 μg/ml) and streptomycin (25 μg/ml), and cultured as 5 × 10 cells per 0.5 ml in 24-well costar dishes (Flow Laboratories, Inc., Mississauga, ON) was added neutral CM or CM that was acidified and subsequently neutralized. .. After 22 hours the cells were pulsed with 0.25 Ci of 5-[125 I] iodo 2′-deoxyuridine (ICN Pharmaceutical, Costa Mesa, CA) for 2 to 3 hours at 37°C and eventually lysed with 1 ml of 1 N NaOH for 30 minutes at room temperature and the 125 I-UdR was counted in a γ counter (LKB Instruments, Gaithersburg, MD).

Article Title: Effect of Lactobacillus fermentum on Beta2 Toxin Production by Clostridium perfringens ▿
Article Snippet: .. Human-derived, enterocyte-like Caco-2 cells ( ) were seeded into 12-well plates (Greiner) at 80,000 cells/cm2 and cultured in Dulbecco's modified Eagle medium (DMEM; Invitrogen) supplemented with 0.03% l -glutamine (Invitrogen), 1% nonessential amino acids (Flow Laboratories), 10 mM sodium bicarbonate, 25 mM HEPES (Flow Laboratories), 50 μg/ml gentamicin (Gibco), and 20% fetal bovine serum (Lonza) in a humid atmosphere of 5% CO2 at 37°C. .. At day 5, the medium was removed and the monolayers were washed twice with 0.01 M phosphate-buffered saline (PBS), pH 7.3, and incubated with plain DMEM.

Article Title: Modification of the Structure and Activity of Lipid A in Yersinia pestis Lipopolysaccharide by Growth Temperature
Article Snippet: Paragraph title: Cell culture. ... For culture of cells, RPMI 1640 medium (Flow Laboratories, Inc., Rockville, Md.) supplemented with 10 mM HEPES, 2 mM l -glutamine, 100 U of penicillin per ml, 100 μg of streptomycin per ml, and 0.2% NaHCO3 was used as the basic medium, and heat-inactivated fetal calf serum (FCS; Flow Laboratories) was added at a concentration of 5 or 10% (5 or 10% FCS-RPMI medium).

Article Title: Cytokine induction by circulating immune complexes and signs of in-vivo complement activation in systemic lupus erythematosus are associated with the occurrence of anti-Sj?gren's syndrome A antibodies
Article Snippet: .. The cell culture media used was RPMI-1640 (Flow Laboratories, Irvine, Scotland, UK) supplemented with 1% glutamine, 1% penicillin streptomycin, 1% HEPES and 1% Ultroser G® (Flow Laboratories). ..

Article Title: Effect of Dirithromycin on Haemophilus influenzae Infection of the Respiratory Mucosa
Article Snippet: .. The strips were dispersed by gentle agitation of the brush in 2 ml of cell culture medium 199 with Earle’s salts and HEPES ( N -hydroxyethylpiperazine- N ′-2-ethanesulfonic acid; Flow Laboratories, Irvine, Scotland, United Kingdom). ..

Activity Assay:

Article Title: Activation of Rat Alveolar Macrophage-Derived Latent Transforming Growth Factor ?-1 by Plasmin Requires Interaction with Thrombospondin-1 and its Cell Surface Receptor, CD36
Article Snippet: Briefly, to subconfluent cells in 0.2% BCP and resuspended in α-MEM, 0.2% BCP, 10 mmol/L Hepes at pH 7.4, penicillin (25 μg/ml) and streptomycin (25 μg/ml), and cultured as 5 × 10 cells per 0.5 ml in 24-well costar dishes (Flow Laboratories, Inc., Mississauga, ON) was added neutral CM or CM that was acidified and subsequently neutralized. .. For confirmation of TGF-β activity, neutralizing monoclonal antibody to TGF-β1–3 (Genzyme) was added before the addition of the CM and resulted in abrogation of all TGF-β activity.

Article Title: Modification of the Structure and Activity of Lipid A in Yersinia pestis Lipopolysaccharide by Growth Temperature
Article Snippet: For culture of cells, RPMI 1640 medium (Flow Laboratories, Inc., Rockville, Md.) supplemented with 10 mM HEPES, 2 mM l -glutamine, 100 U of penicillin per ml, 100 μg of streptomycin per ml, and 0.2% NaHCO3 was used as the basic medium, and heat-inactivated fetal calf serum (FCS; Flow Laboratories) was added at a concentration of 5 or 10% (5 or 10% FCS-RPMI medium). .. The culture supernatant obtained at 24 h after stimulation was assayed for TNF-α activity.

Microscopy:

Article Title: Effect of Dirithromycin on Haemophilus influenzae Infection of the Respiratory Mucosa
Article Snippet: The strips were dispersed by gentle agitation of the brush in 2 ml of cell culture medium 199 with Earle’s salts and HEPES ( N -hydroxyethylpiperazine- N ′-2-ethanesulfonic acid; Flow Laboratories, Irvine, Scotland, United Kingdom). .. For each experiment ( n = 6), three sealed microscope coverslip-slide preparations containing approximately equal numbers of strips of nasal epithelium were constructed: medium 199 alone (control), SH9 culture filtrate (1:1) with epithelium in medium 199, and SH9 culture filtrate (1:1) with epithelium preincubated with dirithromycin in medium 199.

Modification:

Article Title: Effect of Lactobacillus fermentum on Beta2 Toxin Production by Clostridium perfringens ▿
Article Snippet: .. Human-derived, enterocyte-like Caco-2 cells ( ) were seeded into 12-well plates (Greiner) at 80,000 cells/cm2 and cultured in Dulbecco's modified Eagle medium (DMEM; Invitrogen) supplemented with 0.03% l -glutamine (Invitrogen), 1% nonessential amino acids (Flow Laboratories), 10 mM sodium bicarbonate, 25 mM HEPES (Flow Laboratories), 50 μg/ml gentamicin (Gibco), and 20% fetal bovine serum (Lonza) in a humid atmosphere of 5% CO2 at 37°C. .. At day 5, the medium was removed and the monolayers were washed twice with 0.01 M phosphate-buffered saline (PBS), pH 7.3, and incubated with plain DMEM.

Staining:

Article Title: ?2-Microglobulin is Required for the Full Expression of Xenobiotic-Induced Systemic Autoimmunity
Article Snippet: Bioproducts, Walkersville, MD) containing 10% FCS (Hyclone Laboratories, Logan, UT), 2 mM L-glutamine (Sigma Chemical Co., St. Louis, MO), 10 mM HEPES (pH 7.2; Flow Laboratories, Irvine, Scotland), 1% non-essential amino acids (Irvine Scientific, Santa Ana, CA), 20 mM sodium carbonate (Fisher Scientific Company, Fair Lawn, NJ), and 20 ug/ml gentamicin sulfate (Irvine Scientific). .. After washing in complete RPMI 1640 and then PBS containing 0.04% horse serum (Gemini Bio-Products, Woodland, CA), cells were incubated in PBS/0.04% horse serum with Fc Block and then stained with combinations of anti-NK1.1-FITC, Thy1-PE, anti-B220-PerCP, anti-CD3-APC, anti-CD4-FITC, or anti-CD8α-PerCP for 30 min at 4°C, or appropriate isotype controls.

Cytometry:

Article Title: ?2-Microglobulin is Required for the Full Expression of Xenobiotic-Induced Systemic Autoimmunity
Article Snippet: Paragraph title: Flow Cytometry ... Bioproducts, Walkersville, MD) containing 10% FCS (Hyclone Laboratories, Logan, UT), 2 mM L-glutamine (Sigma Chemical Co., St. Louis, MO), 10 mM HEPES (pH 7.2; Flow Laboratories, Irvine, Scotland), 1% non-essential amino acids (Irvine Scientific, Santa Ana, CA), 20 mM sodium carbonate (Fisher Scientific Company, Fair Lawn, NJ), and 20 ug/ml gentamicin sulfate (Irvine Scientific).

other:

Article Title: Immune complexes from rheumatoid arthritis synovial fluid induce Fc?RIIa dependent and rheumatoid factor correlated production of tumour necrosis factor-? by peripheral blood mononuclear cells
Article Snippet: Following two washings in PBS, the cells were counted and diluted to 1 × 106 cells/ml in RPMI-1640 (Flow Laboratories, Irvine, Scotland, UK) supplemented with 1% glutamine, 1% penicillin streptomycin, 1% HEPES and 1% Ultroser G® (Flow Laboratories).

Blocking Assay:

Article Title: ?2-Microglobulin is Required for the Full Expression of Xenobiotic-Induced Systemic Autoimmunity
Article Snippet: Bioproducts, Walkersville, MD) containing 10% FCS (Hyclone Laboratories, Logan, UT), 2 mM L-glutamine (Sigma Chemical Co., St. Louis, MO), 10 mM HEPES (pH 7.2; Flow Laboratories, Irvine, Scotland), 1% non-essential amino acids (Irvine Scientific, Santa Ana, CA), 20 mM sodium carbonate (Fisher Scientific Company, Fair Lawn, NJ), and 20 ug/ml gentamicin sulfate (Irvine Scientific). .. After washing in complete RPMI 1640 and then PBS containing 0.04% horse serum (Gemini Bio-Products, Woodland, CA), cells were incubated in PBS/0.04% horse serum with Fc Block and then stained with combinations of anti-NK1.1-FITC, Thy1-PE, anti-B220-PerCP, anti-CD3-APC, anti-CD4-FITC, or anti-CD8α-PerCP for 30 min at 4°C, or appropriate isotype controls.

Generated:

Article Title: Influence of Mycobacterium bovis Bacillus Calmette Gu?rin on In Vitro Induction of CD1 Molecules in Human Adherent Mononuclear Cells
Article Snippet: The CD1b-transfected C1R.b6 lymphoblastoid cell line was generated by stably transfecting human lymphoblastoid C1R cells with pSRα-NEO vector containing CD1b cDNA, as previously described ( ). .. Lymphoblastoid cells were grown in suspension in RPMI 1640 medium (GIBCO, Paisley, Scotland) supplemented with 10% fetal calf serum (GIBCO), 10 mM HEPES (Flow Laboratories, McLean, Va.), and 2 mM l -glutamine (Flow) and subcultured two or three times weekly.

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    Flow Laboratories hepes
    Hepes, supplied by Flow Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Flow Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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