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EM Science Inc hepes
Hepes, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hepes/product/EM Science Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hepes - by Bioz Stars, 2020-04
86/100 stars

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Electron Microscopy:

Article Title: Three Repeat Isoforms of Tau Inhibit Assembly of Four Repeat Tau Filaments
Article Snippet: .. Electron microscopy of filamentous tau Reaction samples were diluted 4× in 10 mM HEPES, and 10 µl was adsorbed onto carbon/formvar-coated 400 mesh copper grids (EM Sciences) for 30 seconds. .. These were then stained with 2% uranyl acetate for 30 seconds, and the grids were examined with a Philips 208S electron microscope (Philips, Hillsboro, OR).

Homogenization:

Article Title: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin
Article Snippet: Homogenization efficiency and integrity of nuclei was checked with microscopy and 4'-6-Diamidino-2-phenylindole (DAPI) staining for nuclei. .. To obtain the cytosolic, mitochondrial and microsomal fractions, tail fin tissue was homogenized in 20 mM HEPES pH 7.5, 0.25 M sucrose, 5 mM Ethylenedinitrilo-tetraacetic acid (EDTA) (EM Science, Gibbstown NJ USA), 1mM DTT, 100 μM PMSF, 4 μg/ml aprotinin, 1 μg/ml leupeptin, 2 μg/ml antipain, 300 μg/ml benzamidine, 10 mM β-glycerophosphate, 0.2 mM Na3 VO4 , 1 mM NaF.

Isolation:

Article Title: Suppression of Ongoing Experimental Arthritis by a Chinese Herbal Formula (Huo-Luo-Xiao-Ling Dan) Involves Changes in Antigen-Induced Immunological and Biochemical Mediators of Inflammation
Article Snippet: Paragraph title: 2.8. Isolation of Synovium-Infiltrating Cells (SIC) and the Preparation of Cell Lysate ... This dissected tissue was digested for 1 h at 37°C in Hanks' balanced salt solution (HBSS) containing 3 mg/mL collagenase 1A (Sigma-Aldrich), 3 mg/mL hyaluronidase IV-S 1 mg/mL (Sigma), 0.1 mg/mL DNase II (Sigma), 1% fetal bovine serum (FBS) (Invitrogen), and 5% HEPES (EM Science).

Article Title: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin
Article Snippet: For the nuclear isolation tissue was homogenized in 20 mM N-[2-Hydroxyethyl]piperazine-N'-[2-ethanosulfonic acid] (HEPES) pH 7.5, 0.25 M sucrose (ACP Chemicals Inc., Montreal Canada), 25 mM KCl, 5 mM MgCl2 (both from BDH chemicals, Toronto Canada), 0.5% Triton X-100, 1mM dithiothreitol (DTT), 2 mM vanadyl ribonucleoside complexes (VRCs), 100 μM phenylmethylsulfonyl fluoride (PMSF), 4 μg/ml aprotinin, 1 μg/ml leupeptin, 2 μg/ml antipain, 300 μg/ml benzamidine, 10 mM β-glycerophosphate, 0.2 mM Na3 VO4 , and 1 mM NaF (all remaining from Sigma-Aldrich). .. To obtain the cytosolic, mitochondrial and microsomal fractions, tail fin tissue was homogenized in 20 mM HEPES pH 7.5, 0.25 M sucrose, 5 mM Ethylenedinitrilo-tetraacetic acid (EDTA) (EM Science, Gibbstown NJ USA), 1mM DTT, 100 μM PMSF, 4 μg/ml aprotinin, 1 μg/ml leupeptin, 2 μg/ml antipain, 300 μg/ml benzamidine, 10 mM β-glycerophosphate, 0.2 mM Na3 VO4 , 1 mM NaF.

Microscopy:

Article Title: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin
Article Snippet: Homogenization efficiency and integrity of nuclei was checked with microscopy and 4'-6-Diamidino-2-phenylindole (DAPI) staining for nuclei. .. To obtain the cytosolic, mitochondrial and microsomal fractions, tail fin tissue was homogenized in 20 mM HEPES pH 7.5, 0.25 M sucrose, 5 mM Ethylenedinitrilo-tetraacetic acid (EDTA) (EM Science, Gibbstown NJ USA), 1mM DTT, 100 μM PMSF, 4 μg/ml aprotinin, 1 μg/ml leupeptin, 2 μg/ml antipain, 300 μg/ml benzamidine, 10 mM β-glycerophosphate, 0.2 mM Na3 VO4 , 1 mM NaF.

Article Title: Three Repeat Isoforms of Tau Inhibit Assembly of Four Repeat Tau Filaments
Article Snippet: Electron microscopy of filamentous tau Reaction samples were diluted 4× in 10 mM HEPES, and 10 µl was adsorbed onto carbon/formvar-coated 400 mesh copper grids (EM Sciences) for 30 seconds. .. These were then stained with 2% uranyl acetate for 30 seconds, and the grids were examined with a Philips 208S electron microscope (Philips, Hillsboro, OR).

Incubation:

Article Title: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin
Article Snippet: The homogenate was incubated on ice with shaking for 30 min then passed through 100 μm nylon mesh cell-strainer. .. To obtain the cytosolic, mitochondrial and microsomal fractions, tail fin tissue was homogenized in 20 mM HEPES pH 7.5, 0.25 M sucrose, 5 mM Ethylenedinitrilo-tetraacetic acid (EDTA) (EM Science, Gibbstown NJ USA), 1mM DTT, 100 μM PMSF, 4 μg/ml aprotinin, 1 μg/ml leupeptin, 2 μg/ml antipain, 300 μg/ml benzamidine, 10 mM β-glycerophosphate, 0.2 mM Na3 VO4 , 1 mM NaF.

Fractionation:

Article Title: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin
Article Snippet: Paragraph title: Subcellular fractionation ... To obtain the cytosolic, mitochondrial and microsomal fractions, tail fin tissue was homogenized in 20 mM HEPES pH 7.5, 0.25 M sucrose, 5 mM Ethylenedinitrilo-tetraacetic acid (EDTA) (EM Science, Gibbstown NJ USA), 1mM DTT, 100 μM PMSF, 4 μg/ml aprotinin, 1 μg/ml leupeptin, 2 μg/ml antipain, 300 μg/ml benzamidine, 10 mM β-glycerophosphate, 0.2 mM Na3 VO4 , 1 mM NaF.

Lysis:

Article Title: Suppression of Ongoing Experimental Arthritis by a Chinese Herbal Formula (Huo-Luo-Xiao-Ling Dan) Involves Changes in Antigen-Induced Immunological and Biochemical Mediators of Inflammation
Article Snippet: This dissected tissue was digested for 1 h at 37°C in Hanks' balanced salt solution (HBSS) containing 3 mg/mL collagenase 1A (Sigma-Aldrich), 3 mg/mL hyaluronidase IV-S 1 mg/mL (Sigma), 0.1 mg/mL DNase II (Sigma), 1% fetal bovine serum (FBS) (Invitrogen), and 5% HEPES (EM Science). .. The collected cells were lysed in 250 μ l lysis buffer (Bio-rad), and the supernate was harvested for testing of cytokine levels.

Staining:

Article Title: Analysis of the Rana catesbeiana tadpole tail fin proteome and phosphoproteome during T3-induced apoptosis: identification of a novel type I keratin
Article Snippet: Homogenization efficiency and integrity of nuclei was checked with microscopy and 4'-6-Diamidino-2-phenylindole (DAPI) staining for nuclei. .. To obtain the cytosolic, mitochondrial and microsomal fractions, tail fin tissue was homogenized in 20 mM HEPES pH 7.5, 0.25 M sucrose, 5 mM Ethylenedinitrilo-tetraacetic acid (EDTA) (EM Science, Gibbstown NJ USA), 1mM DTT, 100 μM PMSF, 4 μg/ml aprotinin, 1 μg/ml leupeptin, 2 μg/ml antipain, 300 μg/ml benzamidine, 10 mM β-glycerophosphate, 0.2 mM Na3 VO4 , 1 mM NaF.

Article Title: Three Repeat Isoforms of Tau Inhibit Assembly of Four Repeat Tau Filaments
Article Snippet: Electron microscopy of filamentous tau Reaction samples were diluted 4× in 10 mM HEPES, and 10 µl was adsorbed onto carbon/formvar-coated 400 mesh copper grids (EM Sciences) for 30 seconds. .. These were then stained with 2% uranyl acetate for 30 seconds, and the grids were examined with a Philips 208S electron microscope (Philips, Hillsboro, OR).

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    EM Science Inc hepes buffer
    Hepes Buffer, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes buffer/product/EM Science Inc
    Average 91 stars, based on 1 article reviews
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    hepes buffer - by Bioz Stars, 2020-04
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    86
    EM Science Inc hepes
    Hepes, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/EM Science Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-04
    86/100 stars
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    85
    EM Science Inc na hepes
    Na Hepes, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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