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CooperSurgical hepes
Hepes, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hepes/product/CooperSurgical
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hepes - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Clone Assay:

Article Title: Characterization of Tetraploid Somatic Cell Nuclear Transfer-Derived Human Embryonic Stem Cells
Article Snippet: Paragraph title: 3. Somatic cell nuclear transfer procedure and embryo culture and derivation of hESCs from cloned blastocysts ... All MII oocytes were pre-incubated in Quinn’s Advantage medium with HEPES (Cooper Surgical) containing caffeine (1.25 mM) and 0.5 μg/mL cytochalasin B for 5 minutes.

Mass Spectrometry:

Article Title: Characterization of secreted proteins of 2-cell mouse embryos cultured in vitro to the blastocyst stage with and without protein supplementation
Article Snippet: For proteomics experiments frozen (propylene glycol/sucrose as cryoprotectants) two-cell mouse embryos obtained from B6D2F1 males × B6C3F1 females (Conception Technologies, San Diego, CA, USA) were thawed and thoroughly washed three times for 5 min in Quinn’s Advantage Medium with HEPES (SAGE, Cooper Surgical, Trumbull, CT, USA) without protein at room temperature before being divided into two culture groups: Krebs without bovine serum albumin (BSA), and Krebs plus BSA (lyophilized 3 %, Sigma Chemical Company, St. Louis, MO). .. Extreme care was taken in media preparation to avoid any contamination; all Krebs media used in these experiments were prepared in-house and subjected to analysis by mass spectrometry to verify absence of any proteins/peptides.

Quantitative RT-PCR:

Article Title: Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres
Article Snippet: Cleavage-stage human embryos were dissembled to single cells in Quinns Advantage Medium with HEPES (Ca and Mg Free; Copper Surgical) plus 10% Human Albumin (Cooper Surgical), while mouse embryos were dissembled in 0.25% trypsin-EDTA (Gibco) plus 10% human albumin both at 37°C with gentle pipetting. .. Individual embryos and single cells were pre-amplified according to the manufacturer's protocol (Fluidigm Corp.) using the CellsDirect One-Step qRT-PCR kit (Invitrogen) and 20× TaqMan gene expression assays (Applied Biosystems, Foster City, CA, USA) or gene-specific primers designed to span exons using Primer-BLAST (NCBI).

Ex Vivo:

Article Title: Developmental potential of human oocytes matured in vitro followed by vitrification and activation
Article Snippet: Ex vivo, visible (size not measured) follicles were aspirated under reduced pressure of 85 mm Hg using a 19-gauge single-lumen aspiration needle (K-OPS-7035-RWH-ET; Cook, Australia), according to the transvaginal aspiration technique described by Chian et al . .. For bilateral oophorectomy, ovaries were transported immediately in IVM (in vitro maturation) medium containing HEPES (Sage; CooperSurgical, USA) to the IVF laboratory (on the same floor of the hospital).

Incubation:

Article Title: Characterization of secreted proteins of 2-cell mouse embryos cultured in vitro to the blastocyst stage with and without protein supplementation
Article Snippet: For proteomics experiments frozen (propylene glycol/sucrose as cryoprotectants) two-cell mouse embryos obtained from B6D2F1 males × B6C3F1 females (Conception Technologies, San Diego, CA, USA) were thawed and thoroughly washed three times for 5 min in Quinn’s Advantage Medium with HEPES (SAGE, Cooper Surgical, Trumbull, CT, USA) without protein at room temperature before being divided into two culture groups: Krebs without bovine serum albumin (BSA), and Krebs plus BSA (lyophilized 3 %, Sigma Chemical Company, St. Louis, MO). .. Viable 2-cell embryos (post-thaw survival rate > 99 %) were incubated at 37 °C in 5 % CO2 (95 % humidity) in 50 μl aliquots for each culture group in duplicate using Falcon Microtest Primaria tissue culture plates (353872, Becton Dickinson, Franklin Lakes, NJ, USA) for 3 days until reaching the blastocyst stage.

Article Title: Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis
Article Snippet: .. To weaken the cellular junctions between blastomeres, embryos were incubated in 60 μl drops of Quinn's Advantage Ca++ /Mg++ -Free Medium with HEPES (CooperSurgical) plus 10% HA for 10 min at 37 °C under mineral oil. ..

Expressing:

Article Title: Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres
Article Snippet: Paragraph title: Single embryo and cell gene expression analysis ... Cleavage-stage human embryos were dissembled to single cells in Quinns Advantage Medium with HEPES (Ca and Mg Free; Copper Surgical) plus 10% Human Albumin (Cooper Surgical), while mouse embryos were dissembled in 0.25% trypsin-EDTA (Gibco) plus 10% human albumin both at 37°C with gentle pipetting.

Modification:

Article Title: Uroplakins play conserved roles in egg fertilization and acquired additional urothelial functions during mammalian divergence
Article Snippet: .. Hormones gonadotropin from pregnant mare serum (PMSG), chorionic gonadotropin human (hCG); hyaluronidase, acidic Tyrode’s solution, reduced l -glutathione (GSH), Src kinase inhibitor SU6656 and Ficoll 400 (Sigma, St. Louis, MO); HEPES-buffered modified human tubal fluid (HTF; Irvine Scientific, Irvine, CA); Research Vitro Fertilization (RVF) medium (Cook Medical, Bloomington, IN); M2 and KSOM media (EMD Millipore Corp., Billerica MA); Ca/Mg-free Quinn’s Advantage medium with HEPES (Cooper Surgical, Trumbull, CT); Src kinase inhibitor SKI606 (Selleck Chemicals, Houston, TX); RNeasy Mini Kit (Qiagen, Valencia, CA); Capacity Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA); Lowicryl K4M (Polysciences, Warrington, PA); glutaraldehyde, paraformaldehyde, sodium cacodylate, osmium tetroxide, and EMbed 812 (Electron Microscopy Sciences, Hatfield, PA); and HQ Silver enhancement kit (Nanoprobes). ..

Electron Microscopy:

Article Title: Uroplakins play conserved roles in egg fertilization and acquired additional urothelial functions during mammalian divergence
Article Snippet: .. Hormones gonadotropin from pregnant mare serum (PMSG), chorionic gonadotropin human (hCG); hyaluronidase, acidic Tyrode’s solution, reduced l -glutathione (GSH), Src kinase inhibitor SU6656 and Ficoll 400 (Sigma, St. Louis, MO); HEPES-buffered modified human tubal fluid (HTF; Irvine Scientific, Irvine, CA); Research Vitro Fertilization (RVF) medium (Cook Medical, Bloomington, IN); M2 and KSOM media (EMD Millipore Corp., Billerica MA); Ca/Mg-free Quinn’s Advantage medium with HEPES (Cooper Surgical, Trumbull, CT); Src kinase inhibitor SKI606 (Selleck Chemicals, Houston, TX); RNeasy Mini Kit (Qiagen, Valencia, CA); Capacity Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA); Lowicryl K4M (Polysciences, Warrington, PA); glutaraldehyde, paraformaldehyde, sodium cacodylate, osmium tetroxide, and EMbed 812 (Electron Microscopy Sciences, Hatfield, PA); and HQ Silver enhancement kit (Nanoprobes). ..

Transferring:

Article Title: Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis
Article Snippet: The zona pellucida was removed from each embryo by transferring the embryos to 200 μl drops of Acidified Tyrode's Solution (Millipore, MA, USA) briefly and then washing in Quinn's Advantage Medium plus 10% HA at 37 °C under mineral oil. .. To weaken the cellular junctions between blastomeres, embryos were incubated in 60 μl drops of Quinn's Advantage Ca++ /Mg++ -Free Medium with HEPES (CooperSurgical) plus 10% HA for 10 min at 37 °C under mineral oil.

Article Title: Characterization of Tetraploid Somatic Cell Nuclear Transfer-Derived Human Embryonic Stem Cells
Article Snippet: All MII oocytes were pre-incubated in Quinn’s Advantage medium with HEPES (Cooper Surgical) containing caffeine (1.25 mM) and 0.5 μg/mL cytochalasin B for 5 minutes. .. For the enucleation, a holding pipette was used to rotate each oocyte so that the spindle complex was positioned between 5 and 6 o’clock.

Cell Culture:

Article Title: Characterization of secreted proteins of 2-cell mouse embryos cultured in vitro to the blastocyst stage with and without protein supplementation
Article Snippet: For proteomics experiments frozen (propylene glycol/sucrose as cryoprotectants) two-cell mouse embryos obtained from B6D2F1 males × B6C3F1 females (Conception Technologies, San Diego, CA, USA) were thawed and thoroughly washed three times for 5 min in Quinn’s Advantage Medium with HEPES (SAGE, Cooper Surgical, Trumbull, CT, USA) without protein at room temperature before being divided into two culture groups: Krebs without bovine serum albumin (BSA), and Krebs plus BSA (lyophilized 3 %, Sigma Chemical Company, St. Louis, MO). .. Typically, 25 embryos were cultured per 50 μl well, and for each duplicate culture media were pooled into 100 μl samples after extraction of embryos (blastocysts) at the end of the culture period of each experiment (late afternoon of day 3, or day 5 of embryo development).

Article Title: Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage
Article Snippet: .. The embryos were then washed in Quinn’s Advantage Medium with Hepes (CooperSurgical) plus 5% serum protein substitute (CooperSurgical) and each transferred to a 60 μl microdrop of Quinn’s advantage cleavage medium (CooperSurgical) supplemented with 10% serum protein substitute under mineral oil (Sigma, St Louis, MO) and cultured at 37 °C with 6% CO2 , 5% O2 and 89% N2 , standard human embryo culture conditions in accordance with current clinical IVF practice. .. Embryos were cultured in custom polystyrene petridishes with 25 individual microwells located in the centre to help track embryo identity during imaging and subsequent handling.

other:

Article Title: Generation of Human Induced Pluripotent Stem Cells Using Epigenetic Regulators Reveals a Germ Cell-Like Identity in Partially Reprogrammed Colonies
Article Snippet: The blastocysts were then washed in Quinn’s Advantage Medium with Hepes (CooperSurgical) plus 5% Serum Protein Substitute (SPS; CooperSurgical) and transferred to a 20μl microdrop of Quinn’s Advantage Blastocyst Medium (CooperSurgical) with 10% SPS under mineral oil (Sigma-Aldrich) for culturing at 37°C with 6% CO2 , 5% O2 and 89% N2 for 2 hours prior to fixation.

Imaging:

Article Title: Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis
Article Snippet: For this purpose, the dish was taken out of the incubator for not more than 5 min to avoid affecting either the culture of the remaining embryos or the time-lapse imaging intervals. .. To weaken the cellular junctions between blastomeres, embryos were incubated in 60 μl drops of Quinn's Advantage Ca++ /Mg++ -Free Medium with HEPES (CooperSurgical) plus 10% HA for 10 min at 37 °C under mineral oil.

Article Title: Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage
Article Snippet: The embryos were then washed in Quinn’s Advantage Medium with Hepes (CooperSurgical) plus 5% serum protein substitute (CooperSurgical) and each transferred to a 60 μl microdrop of Quinn’s advantage cleavage medium (CooperSurgical) supplemented with 10% serum protein substitute under mineral oil (Sigma, St Louis, MO) and cultured at 37 °C with 6% CO2 , 5% O2 and 89% N2 , standard human embryo culture conditions in accordance with current clinical IVF practice. .. Embryos were cultured in custom polystyrene petridishes with 25 individual microwells located in the centre to help track embryo identity during imaging and subsequent handling.

Inverted Microscopy:

Article Title: Developmental potential of human oocytes matured in vitro followed by vitrification and activation
Article Snippet: For bilateral oophorectomy, ovaries were transported immediately in IVM (in vitro maturation) medium containing HEPES (Sage; CooperSurgical, USA) to the IVF laboratory (on the same floor of the hospital). .. The collected fluid was examined for oocyte-cumulus-complex under a stereomicroscope, and maturity was determined under an inverted microscope.

Article Title: Characterization of Tetraploid Somatic Cell Nuclear Transfer-Derived Human Embryonic Stem Cells
Article Snippet: Somatic cell nuclear transfer procedure and embryo culture and derivation of hESCs from cloned blastocysts Human tetraploid SCNT-hESCs can be acquired during traditional SCNT procedure under an inverted microscope equipped with a Polscope (Oosight, Cambridge Research & Instrumentation, UK) to visualize the spindle complex ( ). .. All MII oocytes were pre-incubated in Quinn’s Advantage medium with HEPES (Cooper Surgical) containing caffeine (1.25 mM) and 0.5 μg/mL cytochalasin B for 5 minutes.

Polymerase Chain Reaction:

Article Title: Single-cell sequencing of primate preimplantation embryos reveals chromosome elimination via cellular fragmentation and blastomere exclusion
Article Snippet: Cleavage-stage embryos were disaggregated into single cells, polar bodies, and cellular fragments if present with Quinn's advantage Ca2+ - and Mg2+ -free medium with HEPES plus 10% human albumin (CooperSurgical) and 0.05% trypsin-EDTA (Thermo Fisher Scientific) as necessary. .. Each blastomere, polar body, and cellular fragment was washed with Ca2+ - and Mg2+ -free PBS and collected individually for transfer to a sterile UltraFlux PCR tube (VWR).

Injection:

Article Title: The Formin Protein mDia2 Serves as a Marker of Spindle Pole Dynamics in Vitrified-Warmed Mouse Oocytes
Article Snippet: Four-week-old ICR mice (Orient-Bio, Kyunggi-do, Korea) were injected with 5 IU of pregnant mare's serum gonadotropin (Sigma-Aldrich) to induce folliculogenesis. .. The cumulus cells were removed by treating the COCs with hyaluronidase (300 µg/ml, Sigma-Aldrich) for 2 min. Metaphase II(MII) oocytes were collected and transferred to Quinn's Advantage® medium containing HEPES (Cooper Surgical, Trumbull, CT, USA) and 20% fetal bovine serum (FBS, Sigma-Aldrich) at 37°C.

Isolation:

Article Title: Characterization of Tetraploid Somatic Cell Nuclear Transfer-Derived Human Embryonic Stem Cells
Article Snippet: All MII oocytes were pre-incubated in Quinn’s Advantage medium with HEPES (Cooper Surgical) containing caffeine (1.25 mM) and 0.5 μg/mL cytochalasin B for 5 minutes. .. After the removal of spindle complex, an isolated DFB cell was aspirated into an insertion pipette, transferred, resuspended in a drop containing HVJ-E extract (Isihara Sangyo Kaisha, Japan), and inserted into the perivitelline space of the enucleated oocytes.

Article Title: Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome
Article Snippet: Paragraph title: Cumulus cells collection and total RNA isolation ... Other CCs were removed by denuding of oocytes in Quinn’s Advantage® Medium with HEPES (CooperSurgical) in presence of 15 IU/ml hyaluronidase (CooperSurgical).

Microscopy:

Article Title: Preclinical evaluation of a new cryopreservation container for a limited number of human spermatozoa
Article Snippet: .. The new container was then placed on a microscope stage and spermatozoa were aspirated using a glass micropipette and then added to Quinn’s Advantage™ Medium with HEPES (Cooper Surgical), supplemented with 10% PPF. ..

Article Title: Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome
Article Snippet: Approximately 5 × 102 CCs were detached from an oocyte with a sharp needle under a dissecting microscope Stemi 2000-C (Carl Zeiss AG, Berlin, Germany) and used for total RNA extraction with a ChargeSwitch RNA Kit (Invitrogen, Paisley, UK). .. Other CCs were removed by denuding of oocytes in Quinn’s Advantage® Medium with HEPES (CooperSurgical) in presence of 15 IU/ml hyaluronidase (CooperSurgical).

Purification:

Article Title: Uroplakins play conserved roles in egg fertilization and acquired additional urothelial functions during mammalian divergence
Article Snippet: Hormones gonadotropin from pregnant mare serum (PMSG), chorionic gonadotropin human (hCG); hyaluronidase, acidic Tyrode’s solution, reduced l -glutathione (GSH), Src kinase inhibitor SU6656 and Ficoll 400 (Sigma, St. Louis, MO); HEPES-buffered modified human tubal fluid (HTF; Irvine Scientific, Irvine, CA); Research Vitro Fertilization (RVF) medium (Cook Medical, Bloomington, IN); M2 and KSOM media (EMD Millipore Corp., Billerica MA); Ca/Mg-free Quinn’s Advantage medium with HEPES (Cooper Surgical, Trumbull, CT); Src kinase inhibitor SKI606 (Selleck Chemicals, Houston, TX); RNeasy Mini Kit (Qiagen, Valencia, CA); Capacity Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA); Lowicryl K4M (Polysciences, Warrington, PA); glutaraldehyde, paraformaldehyde, sodium cacodylate, osmium tetroxide, and EMbed 812 (Electron Microscopy Sciences, Hatfield, PA); and HQ Silver enhancement kit (Nanoprobes). .. All our mouse antibodies were purified using Protein G–coupled Sepharose beads, and rabbit antibodies were immunoaffinity-purified using gel-purified uroplakins or synthetic peptides.

Sequencing:

Article Title: Single-cell sequencing of primate preimplantation embryos reveals chromosome elimination via cellular fragmentation and blastomere exclusion
Article Snippet: Cleavage-stage embryos were disaggregated into single cells, polar bodies, and cellular fragments if present with Quinn's advantage Ca2+ - and Mg2+ -free medium with HEPES plus 10% human albumin (CooperSurgical) and 0.05% trypsin-EDTA (Thermo Fisher Scientific) as necessary. .. Only embryos for which the disassembly process occurred effectively with no apparent loss of material were carried forward for library preparation and sequencing.

Mouse Assay:

Article Title: The Formin Protein mDia2 Serves as a Marker of Spindle Pole Dynamics in Vitrified-Warmed Mouse Oocytes
Article Snippet: At 48 h post-injection, the mice received 5 IU human chorionic gonadotropin (hCG). .. The cumulus cells were removed by treating the COCs with hyaluronidase (300 µg/ml, Sigma-Aldrich) for 2 min. Metaphase II(MII) oocytes were collected and transferred to Quinn's Advantage® medium containing HEPES (Cooper Surgical, Trumbull, CT, USA) and 20% fetal bovine serum (FBS, Sigma-Aldrich) at 37°C.

Software:

Article Title: Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres
Article Snippet: Cleavage-stage human embryos were dissembled to single cells in Quinns Advantage Medium with HEPES (Ca and Mg Free; Copper Surgical) plus 10% Human Albumin (Cooper Surgical), while mouse embryos were dissembled in 0.25% trypsin-EDTA (Gibco) plus 10% human albumin both at 37°C with gentle pipetting. .. Calculated normalized relative quantity (CNRQ) values were calculated and normalized to the two or three most stable housekeeping genes using the qBasePlus 1.3 analysis software ( http://www.biogazelle.com ) as previously described ( ) and graphed using Gene-E ( http://www.broadinstitute.org/cancer/software/GENE-E/ ).

RNA Extraction:

Article Title: Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome
Article Snippet: Approximately 5 × 102 CCs were detached from an oocyte with a sharp needle under a dissecting microscope Stemi 2000-C (Carl Zeiss AG, Berlin, Germany) and used for total RNA extraction with a ChargeSwitch RNA Kit (Invitrogen, Paisley, UK). .. Other CCs were removed by denuding of oocytes in Quinn’s Advantage® Medium with HEPES (CooperSurgical) in presence of 15 IU/ml hyaluronidase (CooperSurgical).

In Vitro:

Article Title: Developmental potential of human oocytes matured in vitro followed by vitrification and activation
Article Snippet: .. For bilateral oophorectomy, ovaries were transported immediately in IVM (in vitro maturation) medium containing HEPES (Sage; CooperSurgical, USA) to the IVF laboratory (on the same floor of the hospital). ..

Embryo Culture:

Article Title: Dynamic blastomere behaviour reflects human embryo ploidy by the four-cell stage
Article Snippet: .. The embryos were then washed in Quinn’s Advantage Medium with Hepes (CooperSurgical) plus 5% serum protein substitute (CooperSurgical) and each transferred to a 60 μl microdrop of Quinn’s advantage cleavage medium (CooperSurgical) supplemented with 10% serum protein substitute under mineral oil (Sigma, St Louis, MO) and cultured at 37 °C with 6% CO2 , 5% O2 and 89% N2 , standard human embryo culture conditions in accordance with current clinical IVF practice. .. Embryos were cultured in custom polystyrene petridishes with 25 individual microwells located in the centre to help track embryo identity during imaging and subsequent handling.

Article Title: Characterization of Tetraploid Somatic Cell Nuclear Transfer-Derived Human Embryonic Stem Cells
Article Snippet: Paragraph title: 3. Somatic cell nuclear transfer procedure and embryo culture and derivation of hESCs from cloned blastocysts ... All MII oocytes were pre-incubated in Quinn’s Advantage medium with HEPES (Cooper Surgical) containing caffeine (1.25 mM) and 0.5 μg/mL cytochalasin B for 5 minutes.

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  • 88
    CooperSurgical hepes coppersurgical
    Hepes Coppersurgical, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes coppersurgical/product/CooperSurgical
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes coppersurgical - by Bioz Stars, 2020-04
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    CooperSurgical hepes buffered holding media
    Hepes Buffered Holding Media, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CooperSurgical hepes buffered medium
    Hepes Buffered Medium, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hepes buffered medium - by Bioz Stars, 2020-04
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    CooperSurgical hepes
    Hepes, supplied by CooperSurgical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/CooperSurgical
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

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