heparitinase i  (Millipore)


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    Structured Review

    Millipore heparitinase i
    3- O -sulphated heparan sulphates interact with tau under pathological conditions . Heparitinase (Hep-ase) treatment decreases membrane-associated and intracellular 3- O -sulphated heparan sulphates (red) staining and co-localization with tau.  Z -projections are depicted for ( A ) control cells; ( B ) SH/ox cells treated with heparitinase before the oxidative stress pulse; and ( C ) SH/hTau P301L  cells. ( D ) Quantification of heparan sulphates (HS) intracellular inclusions in SH/hTau P301L  cells treated with hep-ase during 1.5 or 6 h. ( E ) FRET-based measurement of the molecular interaction between 3- O -sulphated heparan sulphates and tau in SH/ox cell lysates at different time points after the stress pulse. Calculated delta F (ΔF) shows a time-dependent molecular interaction abolished when cells were cultured in the presence of heparitinase I, II, III cocktail (Hep-ase). ( F ) ELISA competition assay showing the higher capacity of heparin, used as a prototype of 3- O -sulphate heparan sulphates, to inhibit the binding of tau to immobilized heparin compared to heparan sulphates. Data represents the mean of three different experiments performed in triplicate each time; error bars show SEM.
    Heparitinase I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparitinase i/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    heparitinase i - by Bioz Stars, 2020-11
    99/100 stars

    Images

    1) Product Images from "HS3ST2 expression is critical for the abnormal phosphorylation of tau in Alzheimer’s disease-related tau pathology"

    Article Title: HS3ST2 expression is critical for the abnormal phosphorylation of tau in Alzheimer’s disease-related tau pathology

    Journal: Brain

    doi: 10.1093/brain/awv056

    3- O -sulphated heparan sulphates interact with tau under pathological conditions . Heparitinase (Hep-ase) treatment decreases membrane-associated and intracellular 3- O -sulphated heparan sulphates (red) staining and co-localization with tau.  Z -projections are depicted for ( A ) control cells; ( B ) SH/ox cells treated with heparitinase before the oxidative stress pulse; and ( C ) SH/hTau P301L  cells. ( D ) Quantification of heparan sulphates (HS) intracellular inclusions in SH/hTau P301L  cells treated with hep-ase during 1.5 or 6 h. ( E ) FRET-based measurement of the molecular interaction between 3- O -sulphated heparan sulphates and tau in SH/ox cell lysates at different time points after the stress pulse. Calculated delta F (ΔF) shows a time-dependent molecular interaction abolished when cells were cultured in the presence of heparitinase I, II, III cocktail (Hep-ase). ( F ) ELISA competition assay showing the higher capacity of heparin, used as a prototype of 3- O -sulphate heparan sulphates, to inhibit the binding of tau to immobilized heparin compared to heparan sulphates. Data represents the mean of three different experiments performed in triplicate each time; error bars show SEM.
    Figure Legend Snippet: 3- O -sulphated heparan sulphates interact with tau under pathological conditions . Heparitinase (Hep-ase) treatment decreases membrane-associated and intracellular 3- O -sulphated heparan sulphates (red) staining and co-localization with tau. Z -projections are depicted for ( A ) control cells; ( B ) SH/ox cells treated with heparitinase before the oxidative stress pulse; and ( C ) SH/hTau P301L cells. ( D ) Quantification of heparan sulphates (HS) intracellular inclusions in SH/hTau P301L cells treated with hep-ase during 1.5 or 6 h. ( E ) FRET-based measurement of the molecular interaction between 3- O -sulphated heparan sulphates and tau in SH/ox cell lysates at different time points after the stress pulse. Calculated delta F (ΔF) shows a time-dependent molecular interaction abolished when cells were cultured in the presence of heparitinase I, II, III cocktail (Hep-ase). ( F ) ELISA competition assay showing the higher capacity of heparin, used as a prototype of 3- O -sulphate heparan sulphates, to inhibit the binding of tau to immobilized heparin compared to heparan sulphates. Data represents the mean of three different experiments performed in triplicate each time; error bars show SEM.

    Techniques Used: Staining, Cell Culture, Enzyme-linked Immunosorbent Assay, Competitive Binding Assay, Binding Assay

    Related Articles

    Transduction:

    Article Title: Cell Surface-Bound TIMP3 Induces Apoptosis in Mesenchymal Cal78 Cells through Ligand-Independent Activation of Death Receptor Signaling and Blockade of Survival Pathways
    Article Snippet: .. Cal78 or Cal78 transduced with LacZ or TIMP3 were cultured and subsequently treated with heparinase I and III (Sigma-Aldrich). .. For the activation of heparinises 4 mM CaCl2 was added.

    MTT Assay:

    Article Title: Heparan Sulfate Mediates Infection of High-Neurovirulence Theiler's Viruses
    Article Snippet: .. Chondroitin sulfate A from sturgeon notocord, heparan sulfate (HS) from bovine kidney, keratan sulfate from bovine cornea, chondroitinase ABC, heparinase, heparitinase II, and mouse anti-HS monoclonal antibody (MAb) F58-10E4 were obtained from Seikagaku America Inc. Heparin, heparinase I and III, neuraminidase, anti-mouse immunoglobulin G (IgG)-fluorescein isothiocyanate (FITC) conjugate, goat anti-mouse polyvalent Ig-FITC, and MTT reagent [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] were obtained from Sigma. ..

    Fluorescence:

    Article Title: Salt overload damages the glycocalyx sodium barrier of vascular endothelium
    Article Snippet: .. Then, cells were incubated in low or high Na+ solution containing Heparinase I (1 U/ml; Sigma-Aldrich Chemie GmbH, Steinheim, Germany) for 20 min, while fluorescence measurements were taken every 10 s. At the end of the experiment, intracellular calibration for sodium was performed as described previously [ ]. .. Extracellular sodium measurements GM7373 cells were cultured as described above.

    Cell Culture:

    Article Title: Cell Surface-Bound TIMP3 Induces Apoptosis in Mesenchymal Cal78 Cells through Ligand-Independent Activation of Death Receptor Signaling and Blockade of Survival Pathways
    Article Snippet: .. Cal78 or Cal78 transduced with LacZ or TIMP3 were cultured and subsequently treated with heparinase I and III (Sigma-Aldrich). .. For the activation of heparinises 4 mM CaCl2 was added.

    Purification:

    Article Title: Regulation of Pathologic Retinal Angiogenesis in Mice and Inhibition of VEGF-VEGFR2 Binding by Soluble Heparan Sulfate
    Article Snippet: .. HS GAGs purified from bovine kidney were from Seikagaku Corp. (Tokyo, Japan) and unfractionated heparin from porcine intestine, heparinase III, heparinase I, and chondoroitinase ABC were from Sigma-Aldrich Corp.. .. The murine plasmin was from Pierce Biotechnology (Rockford, IL, USA) The ocular fluid was collected and pooled from multiple porcine eyes (8-month-old animals) within 3 hours after the pigs were killed at a local slaughterhouse.

    Incubation:

    Article Title: Salt overload damages the glycocalyx sodium barrier of vascular endothelium
    Article Snippet: .. Then, cells were incubated in low or high Na+ solution containing Heparinase I (1 U/ml; Sigma-Aldrich Chemie GmbH, Steinheim, Germany) for 20 min, while fluorescence measurements were taken every 10 s. At the end of the experiment, intracellular calibration for sodium was performed as described previously [ ]. .. Extracellular sodium measurements GM7373 cells were cultured as described above.

    Article Title: Filoviruses Utilize Glycosaminoglycans for Their Attachment to Target Cells
    Article Snippet: .. Medium was removed, and the cells were incubated with heparinase I from Flavobacterium heparinum (Sigma, Wisconsin) in 20 mM Tris-HCl (pH 7.5), 4 mM CaCl, 50 mM NaCl, and 0.01% bovine serum albumin (BSA) or heparinase III from Flavobacterium heparinum (Sigma) in 20 mM Tris-HCl (pH 7.5), 4 mM CaCl, and 0.01% BSA for 1 h at room temperature (RT) and then washed twice with PBS. .. To test the effect on HIV pseudotype infectivities, we then added the appropriate amounts of viral supernatant in 50 μl of medium and incubated for 2 h at 37°C.

    other:

    Article Title: Syndecan-3 is a dendritic cell-specific attachment receptor for HIV-1
    Article Snippet: In the heparinase treatment, 5 × 106 cells per ml were treated with 10 milliunits heparinase I, II, and III (IBEX, Montreal, Canada) or heparinase I (Sigma–Aldrich, St. Louis, MO) in PBS/0.25% BSA for 1 h at 25°C.

    Infection:

    Article Title: Human Papillomavirus Infection Requires Cell Surface Heparan Sulfate
    Article Snippet: .. We therefore digested cell surface-bound heparan sulfate by treating COS-7 cells with heparinase I (Sigma Aldrich) prior to infection. .. COS-7 cells were grown to 80% confluency in Dulbecco's modified Eagle's medium (DMEM; Gibco-BRL) and washed once with 20 mM Tris-HCl–50 mM NaCl–4 mM CaCl2 –0.01% BSA (pH 7.5).

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    Millipore heparinase i from flavobacterium heparinum
    Heparinase I From Flavobacterium Heparinum, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparinase i from flavobacterium heparinum/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heparinase i from flavobacterium heparinum - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

    89
    Millipore heparitinase i
    Heparitinase I, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparitinase i/product/Millipore
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    heparitinase i - by Bioz Stars, 2020-11
    89/100 stars
      Buy from Supplier

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