Structured Review

IBEX Technologies glycocalyx configuration
En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the <t>glycocalyx</t> when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P
Glycocalyx Configuration, supplied by IBEX Technologies, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glycocalyx configuration/product/IBEX Technologies
Average 90 stars, based on 5 article reviews
Price from $9.99 to $1999.99
glycocalyx configuration - by Bioz Stars, 2020-11
90/100 stars

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1) Product Images from "Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting"

Article Title: Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S106299

En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P
Figure Legend Snippet: En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P

Techniques Used: Binding Assay

En face and cross sections of RFPEC in which BSA was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Quantification of the overall thickness of glycocalyx after various treatments – control, low serum, Hep III, Hep III + HS – depicted by staining BSA adsorbed onto the glycosaminoglycan matrix. The glycocalyx treatments and recovery decreased overall thickness. ( F ) The glycocalyx coverage as seen by BSA immunofluorescence. The various treatments and glycocalyx conditions did not affect overall glycocalyx coverage. * P
Figure Legend Snippet: En face and cross sections of RFPEC in which BSA was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Quantification of the overall thickness of glycocalyx after various treatments – control, low serum, Hep III, Hep III + HS – depicted by staining BSA adsorbed onto the glycosaminoglycan matrix. The glycocalyx treatments and recovery decreased overall thickness. ( F ) The glycocalyx coverage as seen by BSA immunofluorescence. The various treatments and glycocalyx conditions did not affect overall glycocalyx coverage. * P

Techniques Used: Staining, Immunofluorescence

Uptake and localization differences between glycocalyx conditions. Notes: ( A ) Confocal cross sections of PEG-AuNP (red) in RFPEC under various glycocalyx conditions. ( B ) Relative fluorescent AuNP uptake by quantifying the amount of red fluorescence in cross-sectional images and normalizing to controls. * P
Figure Legend Snippet: Uptake and localization differences between glycocalyx conditions. Notes: ( A ) Confocal cross sections of PEG-AuNP (red) in RFPEC under various glycocalyx conditions. ( B ) Relative fluorescent AuNP uptake by quantifying the amount of red fluorescence in cross-sectional images and normalizing to controls. * P

Techniques Used: Fluorescence

2) Product Images from "Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting"

Article Title: Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S106299

En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P
Figure Legend Snippet: En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P

Techniques Used: Binding Assay

Related Articles

Incubation:

Article Title: De Novo Sequencing of Complex Mixtures of Heparan Sulfate Oligosaccharides
Article Snippet: .. The solution was incubated at 37 °C for a total of 48 h, with an additional aliquot of 0.05 IU heparinase III added after 24 h incubation. .. The enzyme digestion was stopped by heating the solution at 100 °C for 10 min.

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