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Stratagene heat competent e coli xl 1 blue cells
Heat Competent E Coli Xl 1 Blue Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heat competent e coli xl 1 blue cells/product/Stratagene
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
heat competent e coli xl 1 blue cells - by Bioz Stars, 2020-04
86/100 stars

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Amplification:

Article Title: Production and characterisation of a recombinant scFv reactive with human gastrointestinal carcinomas
Article Snippet: .. This vector was amplified in heat competent E. coli XL-1 blue cells (Stratagene, La Jolla, CA, USA) and then extracted with a QIAprep spin miniprep kit (Qiagen). .. This vector was used to transform competent BL21 E. coli cells (Novagen, Madison, WI, USA) using a heat shock (42°C) transformation method.

Ligation:

Article Title: Production and characterisation of a recombinant scFv reactive with human gastrointestinal carcinomas
Article Snippet: This vector was digested with Hind III restriction enzyme and ligated using a ligation kit (Gibco-BRL, Grand Island, NY, USA). .. This vector was amplified in heat competent E. coli XL-1 blue cells (Stratagene, La Jolla, CA, USA) and then extracted with a QIAprep spin miniprep kit (Qiagen).

Purification:

Article Title: Production and characterisation of a recombinant scFv reactive with human gastrointestinal carcinomas
Article Snippet: The DNA was purified using the Qiaex II gel extraction kit (Qiagen, Stanford Valencia, CA, USA). .. This vector was amplified in heat competent E. coli XL-1 blue cells (Stratagene, La Jolla, CA, USA) and then extracted with a QIAprep spin miniprep kit (Qiagen).

Gel Extraction:

Article Title: Production and characterisation of a recombinant scFv reactive with human gastrointestinal carcinomas
Article Snippet: The DNA was purified using the Qiaex II gel extraction kit (Qiagen, Stanford Valencia, CA, USA). .. This vector was amplified in heat competent E. coli XL-1 blue cells (Stratagene, La Jolla, CA, USA) and then extracted with a QIAprep spin miniprep kit (Qiagen).

Transformation Assay:

Article Title: Production and characterisation of a recombinant scFv reactive with human gastrointestinal carcinomas
Article Snippet: This vector was amplified in heat competent E. coli XL-1 blue cells (Stratagene, La Jolla, CA, USA) and then extracted with a QIAprep spin miniprep kit (Qiagen). .. This vector was used to transform competent BL21 E. coli cells (Novagen, Madison, WI, USA) using a heat shock (42°C) transformation method.

Recombinant:

Article Title: Production and characterisation of a recombinant scFv reactive with human gastrointestinal carcinomas
Article Snippet: This vector was amplified in heat competent E. coli XL-1 blue cells (Stratagene, La Jolla, CA, USA) and then extracted with a QIAprep spin miniprep kit (Qiagen). .. Transformed BL21 E. coli were then subjected to culture protocol, producing a recombinant scFv of SC142 encoding VH- and VL-linked genes.

Plasmid Preparation:

Article Title: Production and characterisation of a recombinant scFv reactive with human gastrointestinal carcinomas
Article Snippet: .. This vector was amplified in heat competent E. coli XL-1 blue cells (Stratagene, La Jolla, CA, USA) and then extracted with a QIAprep spin miniprep kit (Qiagen). .. This vector was used to transform competent BL21 E. coli cells (Novagen, Madison, WI, USA) using a heat shock (42°C) transformation method.

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    Stratagene heat competent e coli xl 1 blue cells
    Heat Competent E Coli Xl 1 Blue Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat competent e coli xl 1 blue cells/product/Stratagene
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heat competent e coli xl 1 blue cells - by Bioz Stars, 2020-04
    86/100 stars
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