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Journal: STAR Protocols
Article Title: Protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined with immunohistochemistry in the mosquito brain
doi: 10.1016/j.xpro.2025.104109
Figure Lengend Snippet: Example of multiplex whole-mount RNA fluorescent HCR in situ hybridization combined with immunohistochemistry in the mosquito brain (A) Whole mosquito brain showing RNA fluorescent HCR in situ hybridization against putative GAD1 ( AGAP005866 ) and SerT ( AGAP010621 ) and immunohistochemistry against PDF and DAPI. (B–B″″) Maximum projection (∼2 μm total) of the optic lobe showing non-overlapping cell bodies of GAD1 + and SerT + cells and PDF neurites. Individual panels show fluorescence channels imaged using the following excitation wavelengths: 488 nm (PDF), 546 nm ( GAD1 ), 647 nm ( SerT ), and 358 nm (DAPI). See also and .
Article Snippet:
Techniques: Multiplex Assay, In Situ Hybridization, Immunohistochemistry, Fluorescence
Journal: STAR Protocols
Article Title: Protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined with immunohistochemistry in the mosquito brain
doi: 10.1016/j.xpro.2025.104109
Figure Lengend Snippet: Example of multiplex whole-mount RNA fluorescent HCR in situ hybridization combined with immunohistochemistry in the mosquito brain (A) Whole mosquito brain showing RNA fluorescent HCR in situ hybridization against putative GAD1 ( AGAP005866 ) and SerT ( AGAP010621 ) and immunohistochemistry against PDF and DAPI. (B–B″″) Maximum projection (∼2 μm total) of the optic lobe showing non-overlapping cell bodies of GAD1 + and SerT + cells and PDF neurites. Individual panels show fluorescence channels imaged using the following excitation wavelengths: 488 nm (PDF), 546 nm ( GAD1 ), 647 nm ( SerT ), and 358 nm (DAPI). See also and .
Article Snippet:
Techniques: Multiplex Assay, In Situ Hybridization, Immunohistochemistry, Fluorescence