hccs e coli strain novablue  (Millipore)


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    Structured Review

    Millipore hccs e coli strain novablue
    Hccs E Coli Strain Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hccs e coli strain novablue/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hccs e coli strain novablue - by Bioz Stars, 2020-04
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
    Article Snippet: .. 3.1.Construction of Plasmids for Co-expression of hSOD1 and hCCS E. coli strain NovaBlue (Novagen, Germany) was used for cloning. hSOD1 (GenBank: EF151142.1) was amplified from the pET20b-hSOD1 , a construct donated by Prof. Daret K. St. Clair, University of Kentucky, using i-Taq polymerase (Intron Biotechnology, South Korea) with forward (5´- ATA CATATG GCGACGAAGGC- 3´, underlined is Nde I restriction site) and reverse (5´-ATT GCTCAGC TTATTGGGCG-3´, underlined is Bpu 1102 I). hCCS (GenBank: NM_005125) was amplified using Gene PoolTM cDNA, from human normal brain tissue (Invitrogen, USA) with forward (5´-TGG CCATGG CTTCGGATTCG- 3´, underlined is Nco I restriction site) and reverse (5´- GAC AAGCTT TCAAAGGTGGG- 3´, underlined is Hind III restriction site). .. The 465 bp and 824 bp PCR products of hSOD1 and hCCS , respectively were digested with the corresponding restriction enzymes and purified from agarose gel after electrophoresis.

    Amplification:

    Article Title: Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
    Article Snippet: .. 3.1.Construction of Plasmids for Co-expression of hSOD1 and hCCS E. coli strain NovaBlue (Novagen, Germany) was used for cloning. hSOD1 (GenBank: EF151142.1) was amplified from the pET20b-hSOD1 , a construct donated by Prof. Daret K. St. Clair, University of Kentucky, using i-Taq polymerase (Intron Biotechnology, South Korea) with forward (5´- ATA CATATG GCGACGAAGGC- 3´, underlined is Nde I restriction site) and reverse (5´-ATT GCTCAGC TTATTGGGCG-3´, underlined is Bpu 1102 I). hCCS (GenBank: NM_005125) was amplified using Gene PoolTM cDNA, from human normal brain tissue (Invitrogen, USA) with forward (5´-TGG CCATGG CTTCGGATTCG- 3´, underlined is Nco I restriction site) and reverse (5´- GAC AAGCTT TCAAAGGTGGG- 3´, underlined is Hind III restriction site). .. The 465 bp and 824 bp PCR products of hSOD1 and hCCS , respectively were digested with the corresponding restriction enzymes and purified from agarose gel after electrophoresis.

    Agarose Gel Electrophoresis:

    Article Title: Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
    Article Snippet: 3.1.Construction of Plasmids for Co-expression of hSOD1 and hCCS E. coli strain NovaBlue (Novagen, Germany) was used for cloning. hSOD1 (GenBank: EF151142.1) was amplified from the pET20b-hSOD1 , a construct donated by Prof. Daret K. St. Clair, University of Kentucky, using i-Taq polymerase (Intron Biotechnology, South Korea) with forward (5´- ATA CATATG GCGACGAAGGC- 3´, underlined is Nde I restriction site) and reverse (5´-ATT GCTCAGC TTATTGGGCG-3´, underlined is Bpu 1102 I). hCCS (GenBank: NM_005125) was amplified using Gene PoolTM cDNA, from human normal brain tissue (Invitrogen, USA) with forward (5´-TGG CCATGG CTTCGGATTCG- 3´, underlined is Nco I restriction site) and reverse (5´- GAC AAGCTT TCAAAGGTGGG- 3´, underlined is Hind III restriction site). .. The 465 bp and 824 bp PCR products of hSOD1 and hCCS , respectively were digested with the corresponding restriction enzymes and purified from agarose gel after electrophoresis.

    Construct:

    Article Title: Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
    Article Snippet: .. 3.1.Construction of Plasmids for Co-expression of hSOD1 and hCCS E. coli strain NovaBlue (Novagen, Germany) was used for cloning. hSOD1 (GenBank: EF151142.1) was amplified from the pET20b-hSOD1 , a construct donated by Prof. Daret K. St. Clair, University of Kentucky, using i-Taq polymerase (Intron Biotechnology, South Korea) with forward (5´- ATA CATATG GCGACGAAGGC- 3´, underlined is Nde I restriction site) and reverse (5´-ATT GCTCAGC TTATTGGGCG-3´, underlined is Bpu 1102 I). hCCS (GenBank: NM_005125) was amplified using Gene PoolTM cDNA, from human normal brain tissue (Invitrogen, USA) with forward (5´-TGG CCATGG CTTCGGATTCG- 3´, underlined is Nco I restriction site) and reverse (5´- GAC AAGCTT TCAAAGGTGGG- 3´, underlined is Hind III restriction site). .. The 465 bp and 824 bp PCR products of hSOD1 and hCCS , respectively were digested with the corresponding restriction enzymes and purified from agarose gel after electrophoresis.

    Purification:

    Article Title: Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
    Article Snippet: 3.1.Construction of Plasmids for Co-expression of hSOD1 and hCCS E. coli strain NovaBlue (Novagen, Germany) was used for cloning. hSOD1 (GenBank: EF151142.1) was amplified from the pET20b-hSOD1 , a construct donated by Prof. Daret K. St. Clair, University of Kentucky, using i-Taq polymerase (Intron Biotechnology, South Korea) with forward (5´- ATA CATATG GCGACGAAGGC- 3´, underlined is Nde I restriction site) and reverse (5´-ATT GCTCAGC TTATTGGGCG-3´, underlined is Bpu 1102 I). hCCS (GenBank: NM_005125) was amplified using Gene PoolTM cDNA, from human normal brain tissue (Invitrogen, USA) with forward (5´-TGG CCATGG CTTCGGATTCG- 3´, underlined is Nco I restriction site) and reverse (5´- GAC AAGCTT TCAAAGGTGGG- 3´, underlined is Hind III restriction site). .. The 465 bp and 824 bp PCR products of hSOD1 and hCCS , respectively were digested with the corresponding restriction enzymes and purified from agarose gel after electrophoresis.

    Electrophoresis:

    Article Title: Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
    Article Snippet: 3.1.Construction of Plasmids for Co-expression of hSOD1 and hCCS E. coli strain NovaBlue (Novagen, Germany) was used for cloning. hSOD1 (GenBank: EF151142.1) was amplified from the pET20b-hSOD1 , a construct donated by Prof. Daret K. St. Clair, University of Kentucky, using i-Taq polymerase (Intron Biotechnology, South Korea) with forward (5´- ATA CATATG GCGACGAAGGC- 3´, underlined is Nde I restriction site) and reverse (5´-ATT GCTCAGC TTATTGGGCG-3´, underlined is Bpu 1102 I). hCCS (GenBank: NM_005125) was amplified using Gene PoolTM cDNA, from human normal brain tissue (Invitrogen, USA) with forward (5´-TGG CCATGG CTTCGGATTCG- 3´, underlined is Nco I restriction site) and reverse (5´- GAC AAGCTT TCAAAGGTGGG- 3´, underlined is Hind III restriction site). .. The 465 bp and 824 bp PCR products of hSOD1 and hCCS , respectively were digested with the corresponding restriction enzymes and purified from agarose gel after electrophoresis.

    Polymerase Chain Reaction:

    Article Title: Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
    Article Snippet: 3.1.Construction of Plasmids for Co-expression of hSOD1 and hCCS E. coli strain NovaBlue (Novagen, Germany) was used for cloning. hSOD1 (GenBank: EF151142.1) was amplified from the pET20b-hSOD1 , a construct donated by Prof. Daret K. St. Clair, University of Kentucky, using i-Taq polymerase (Intron Biotechnology, South Korea) with forward (5´- ATA CATATG GCGACGAAGGC- 3´, underlined is Nde I restriction site) and reverse (5´-ATT GCTCAGC TTATTGGGCG-3´, underlined is Bpu 1102 I). hCCS (GenBank: NM_005125) was amplified using Gene PoolTM cDNA, from human normal brain tissue (Invitrogen, USA) with forward (5´-TGG CCATGG CTTCGGATTCG- 3´, underlined is Nco I restriction site) and reverse (5´- GAC AAGCTT TCAAAGGTGGG- 3´, underlined is Hind III restriction site). .. The 465 bp and 824 bp PCR products of hSOD1 and hCCS , respectively were digested with the corresponding restriction enzymes and purified from agarose gel after electrophoresis.

    Plasmid Preparation:

    Article Title: Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
    Article Snippet: 3.1.Construction of Plasmids for Co-expression of hSOD1 and hCCS E. coli strain NovaBlue (Novagen, Germany) was used for cloning. hSOD1 (GenBank: EF151142.1) was amplified from the pET20b-hSOD1 , a construct donated by Prof. Daret K. St. Clair, University of Kentucky, using i-Taq polymerase (Intron Biotechnology, South Korea) with forward (5´- ATA CATATG GCGACGAAGGC- 3´, underlined is Nde I restriction site) and reverse (5´-ATT GCTCAGC TTATTGGGCG-3´, underlined is Bpu 1102 I). hCCS (GenBank: NM_005125) was amplified using Gene PoolTM cDNA, from human normal brain tissue (Invitrogen, USA) with forward (5´-TGG CCATGG CTTCGGATTCG- 3´, underlined is Nco I restriction site) and reverse (5´- GAC AAGCTT TCAAAGGTGGG- 3´, underlined is Hind III restriction site). .. The hSOD1 was ligated into plasmid pETDuet-1 (Novagen, Germany) at multiple cloning site2 (MCS2) to obtain pETDuet-1-hSOD1 .