hc t4 ligase  (Thermo Fisher)


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    Name:
    T4 DNA Ligase Buffer
    Description:
    T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double stranded DNAs with 3 hydroxyl and 5 phosphate termini The unique T4 DNA Ligase buffer optimizes ligation which can be performed in 5 minutes 1 Single stranded nucleic acids are not substrates for this enzyme A T4 DNA Ligase Technical Bulletin is available Applications Cloning blunt end or cohesive end ligation 2 Adding linkers or adapters to blunt ended DNA 2 Source Purified from E coli œ lysogen NM989 Performance and Quality Testing Endodeoxyribonuclease 3 and 5 exodeoxyribonuclease assays ligation efficiency tested Unit Definition One unit catalyzes the exchange of 1 nmol 32P labeled pyrophosphate into ATP in 20 min at 37°C One unit is equal to approximately 300 cohesive end ligation units Unit Reaction Conditions 66 mM Tris HCl pH 7 6 6 6 mM MgCl2 10 mM DTT 66 µM ATP 3 3 µM 32 P labeled pyrophosphate and enzyme in 0 1 ml for 20 min at 37°C
    Catalog Number:
    46300018
    Price:
    None
    Applications:
    ChIP-on-Chip|Cloning|RNAi, Epigenetics & Non-Coding RNA Research|Chromatin Biology|Restriction Enzyme Cloning
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher hc t4 ligase
    T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double stranded DNAs with 3 hydroxyl and 5 phosphate termini The unique T4 DNA Ligase buffer optimizes ligation which can be performed in 5 minutes 1 Single stranded nucleic acids are not substrates for this enzyme A T4 DNA Ligase Technical Bulletin is available Applications Cloning blunt end or cohesive end ligation 2 Adding linkers or adapters to blunt ended DNA 2 Source Purified from E coli œ lysogen NM989 Performance and Quality Testing Endodeoxyribonuclease 3 and 5 exodeoxyribonuclease assays ligation efficiency tested Unit Definition One unit catalyzes the exchange of 1 nmol 32P labeled pyrophosphate into ATP in 20 min at 37°C One unit is equal to approximately 300 cohesive end ligation units Unit Reaction Conditions 66 mM Tris HCl pH 7 6 6 6 mM MgCl2 10 mM DTT 66 µM ATP 3 3 µM 32 P labeled pyrophosphate and enzyme in 0 1 ml for 20 min at 37°C
    https://www.bioz.com/result/hc t4 ligase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hc t4 ligase - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Assembly of custom TALE-type DNA binding domains by modular cloning
    Article Snippet: .. Cut-ligation cloning protocol For cut-ligation reaction 40 fmol of each plasmid, ligation buffer (Fermentas), 15 U of either Bsa I or Bpi I and 15 U high-concentrated T4 DNA ligase were used in a 20 μl volume. .. One microliter reaction mix was added to 50 μl chemical competent TOP10 cells, incubated for 15 min on ice and transformed by heat shock.

    Incubation:

    Article Title: Extract of Nippostrongylus brasiliensis Stimulates Polyclonal Type-2 Immunoglobulin Response by Inducing De Novo Class Switch
    Article Snippet: .. The reaction mixtures were then incubated overnight in a 37°C waterbath, following which the enzyme was inactivated by incubation at 70°C for 20 min. For ligation (circularization), 10 to 20 μl of digested DNA samples was placed in 1.5-ml microcentrifuge tubes to which 20 μl of 5× T4 DNA ligase buffer (1× final; Life Technologies), 2 μl (20 U) of T4 DNA ligase (Life Technologies), and double-distilled water were added to a final volume of 100 μl. .. The reaction mixtures were incubated overnight in a 16°C waterbath.

    Article Title: A Rapid Cloning Method Employing Orthogonal End Protection
    Article Snippet: .. Equal molar amounts (typically 250–500 ng at ∼ 100 – 250 ng/µl ) of orthogonally protected synthons were mixed, 0.5–1 unit T4 ligase (Fermentas) and T4 ligase buffer (Fermentas) were added and the ligation mixture was incubated for 10–20 min at 16°C. .. Adding additional ligase had little effect on ligation efficiency.

    Article Title: Establishing broad generality of DNA catalysts for site-specific hydrolysis of single-stranded DNA
    Article Snippet: .. The sample was then brought to 20 µl total volume containing 1× of T4 DNA ligase buffer and one unit of T4 DNA ligase (Fermentas), incubated for 1 h at 37°C, and separated by 8% PAGE. ..

    Plasmid Preparation:

    Article Title: Assembly of custom TALE-type DNA binding domains by modular cloning
    Article Snippet: .. Cut-ligation cloning protocol For cut-ligation reaction 40 fmol of each plasmid, ligation buffer (Fermentas), 15 U of either Bsa I or Bpi I and 15 U high-concentrated T4 DNA ligase were used in a 20 μl volume. .. One microliter reaction mix was added to 50 μl chemical competent TOP10 cells, incubated for 15 min on ice and transformed by heat shock.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Establishing broad generality of DNA catalysts for site-specific hydrolysis of single-stranded DNA
    Article Snippet: .. The sample was then brought to 20 µl total volume containing 1× of T4 DNA ligase buffer and one unit of T4 DNA ligase (Fermentas), incubated for 1 h at 37°C, and separated by 8% PAGE. ..

    Ligation:

    Article Title: PARALLEL EVOLUTION OF LOCAL ADAPTATION AND REPRODUCTIVE ISOLATION IN THE FACE OF GENE FLOW
    Article Snippet: .. Then, 5.5 μL of 1× ligation buffer (Invitrogen, Carlsbad, CA) containing 0.5U of T4 DNA ligase (Invitrogen), 0.9 μM Eco-adaptor, and 0.9 μM Pst-adaptor were added to the digestion reaction. .. Ligation reactions were diluted 1:4 and used as template for preselective PCRs.

    Article Title: Dissolution of Xylose Metabolism in Lactococcus lactis
    Article Snippet: .. Ligation reactions contained restricted DNA at a series of concentrations from 25 to 750 ng, together with 1 U of T4 DNA ligase (Gibco, Gaithersburg, Md.), 10 μl of 5× ligation buffer (Gibco), and sterile distilled H2 O, for a final reaction volume of 50 μl. .. The resulting self-ligated DNA was then precipitated with NaCl and ethanol and then resuspended in sterile distilled H2 O to give a final concentration of 5 ng/μl for use in PCR.

    Article Title: Extract of Nippostrongylus brasiliensis Stimulates Polyclonal Type-2 Immunoglobulin Response by Inducing De Novo Class Switch
    Article Snippet: .. The reaction mixtures were then incubated overnight in a 37°C waterbath, following which the enzyme was inactivated by incubation at 70°C for 20 min. For ligation (circularization), 10 to 20 μl of digested DNA samples was placed in 1.5-ml microcentrifuge tubes to which 20 μl of 5× T4 DNA ligase buffer (1× final; Life Technologies), 2 μl (20 U) of T4 DNA ligase (Life Technologies), and double-distilled water were added to a final volume of 100 μl. .. The reaction mixtures were incubated overnight in a 16°C waterbath.

    Article Title: A Rapid Cloning Method Employing Orthogonal End Protection
    Article Snippet: .. Equal molar amounts (typically 250–500 ng at ∼ 100 – 250 ng/µl ) of orthogonally protected synthons were mixed, 0.5–1 unit T4 ligase (Fermentas) and T4 ligase buffer (Fermentas) were added and the ligation mixture was incubated for 10–20 min at 16°C. .. Adding additional ligase had little effect on ligation efficiency.

    Article Title: SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
    Article Snippet: .. For the 3΄-end ligation; adapter ss1 (final conc 10 μM), T4 DNA ligase buffer (40 mM Tris–HCl pH 7.8,10 mM MgCl2 , 10 mM DTT, 0.5 mM ATP), PEG4000 (5% w/v) and 30 units T4 DNA ligase (Thermo Fisher Scientific) and nuclease free water was added to the sample on ice, in a total volume of 30 μl. .. Ligation reaction mixtures were incubated at 20°C for 1 h, and subsequently purified using AMPure XP (BeckmanCoulter) beads in a 2:1 bead to sample ratio and eluted with 10 μl nuclease free water.

    Article Title: Assembly of custom TALE-type DNA binding domains by modular cloning
    Article Snippet: .. Cut-ligation cloning protocol For cut-ligation reaction 40 fmol of each plasmid, ligation buffer (Fermentas), 15 U of either Bsa I or Bpi I and 15 U high-concentrated T4 DNA ligase were used in a 20 μl volume. .. One microliter reaction mix was added to 50 μl chemical competent TOP10 cells, incubated for 15 min on ice and transformed by heat shock.

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  • 99
    Thermo Fisher t4 dna ligase hc
    T4 Dna Ligase Hc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase hc/product/Thermo Fisher
    Average 99 stars, based on 557 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase hc - by Bioz Stars, 2020-09
    99/100 stars
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