hc t4 ligase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hc t4 ligase
    Hc T4 Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hc t4 ligase/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hc t4 ligase - by Bioz Stars, 2020-05
    96/100 stars

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    Related Articles

    Ligation:

    Article Title: Extract of Nippostrongylus brasiliensis Stimulates Polyclonal Type-2 Immunoglobulin Response by Inducing De Novo Class Switch
    Article Snippet: .. The reaction mixtures were then incubated overnight in a 37°C waterbath, following which the enzyme was inactivated by incubation at 70°C for 20 min. For ligation (circularization), 10 to 20 μl of digested DNA samples was placed in 1.5-ml microcentrifuge tubes to which 20 μl of 5× T4 DNA ligase buffer (1× final; Life Technologies), 2 μl (20 U) of T4 DNA ligase (Life Technologies), and double-distilled water were added to a final volume of 100 μl. .. The reaction mixtures were incubated overnight in a 16°C waterbath.

    Article Title: Optimizing splinted ligation of highly structured small RNAs
    Article Snippet: .. Standard ligation conditions included T4 DNA ligase (a stock solution of 30 Weiss units/μL was from Fermentas) at a final concentration of 1 unit/μL and incubation for 3 h at 30° C unless specified otherwise. .. DNA digestion was performed by incubation with 5 units of DNase I (Roche Applied Science) for 15 min at 30 ° C. For analysis of ligation reactions, 20 μL of the ligation batch was mixed with 20 μL of dye-free loading buffer (90% formamide, 1xTBE), heat-denatured, and submitted to urea-PAGE.

    Article Title: SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
    Article Snippet: .. For the 3΄-end ligation; adapter ss1 (final conc 10 μM), T4 DNA ligase buffer (40 mM Tris–HCl pH 7.8,10 mM MgCl2 , 10 mM DTT, 0.5 mM ATP), PEG4000 (5% w/v) and 30 units T4 DNA ligase (Thermo Fisher Scientific) and nuclease free water was added to the sample on ice, in a total volume of 30 μl. .. Ligation reaction mixtures were incubated at 20°C for 1 h, and subsequently purified using AMPure XP (BeckmanCoulter) beads in a 2:1 bead to sample ratio and eluted with 10 μl nuclease free water.

    Article Title: A Rapid Cloning Method Employing Orthogonal End Protection
    Article Snippet: .. Equal molar amounts (typically 250–500 ng at ∼ 100 – 250 ng/µl ) of orthogonally protected synthons were mixed, 0.5–1 unit T4 ligase (Fermentas) and T4 ligase buffer (Fermentas) were added and the ligation mixture was incubated for 10–20 min at 16°C. .. Adding additional ligase had little effect on ligation efficiency.

    Concentration Assay:

    Article Title: Optimizing splinted ligation of highly structured small RNAs
    Article Snippet: .. Standard ligation conditions included T4 DNA ligase (a stock solution of 30 Weiss units/μL was from Fermentas) at a final concentration of 1 unit/μL and incubation for 3 h at 30° C unless specified otherwise. .. DNA digestion was performed by incubation with 5 units of DNase I (Roche Applied Science) for 15 min at 30 ° C. For analysis of ligation reactions, 20 μL of the ligation batch was mixed with 20 μL of dye-free loading buffer (90% formamide, 1xTBE), heat-denatured, and submitted to urea-PAGE.

    Incubation:

    Article Title: Extract of Nippostrongylus brasiliensis Stimulates Polyclonal Type-2 Immunoglobulin Response by Inducing De Novo Class Switch
    Article Snippet: .. The reaction mixtures were then incubated overnight in a 37°C waterbath, following which the enzyme was inactivated by incubation at 70°C for 20 min. For ligation (circularization), 10 to 20 μl of digested DNA samples was placed in 1.5-ml microcentrifuge tubes to which 20 μl of 5× T4 DNA ligase buffer (1× final; Life Technologies), 2 μl (20 U) of T4 DNA ligase (Life Technologies), and double-distilled water were added to a final volume of 100 μl. .. The reaction mixtures were incubated overnight in a 16°C waterbath.

    Article Title: Optimizing splinted ligation of highly structured small RNAs
    Article Snippet: .. Standard ligation conditions included T4 DNA ligase (a stock solution of 30 Weiss units/μL was from Fermentas) at a final concentration of 1 unit/μL and incubation for 3 h at 30° C unless specified otherwise. .. DNA digestion was performed by incubation with 5 units of DNase I (Roche Applied Science) for 15 min at 30 ° C. For analysis of ligation reactions, 20 μL of the ligation batch was mixed with 20 μL of dye-free loading buffer (90% formamide, 1xTBE), heat-denatured, and submitted to urea-PAGE.

    Article Title: A Rapid Cloning Method Employing Orthogonal End Protection
    Article Snippet: .. Equal molar amounts (typically 250–500 ng at ∼ 100 – 250 ng/µl ) of orthogonally protected synthons were mixed, 0.5–1 unit T4 ligase (Fermentas) and T4 ligase buffer (Fermentas) were added and the ligation mixture was incubated for 10–20 min at 16°C. .. Adding additional ligase had little effect on ligation efficiency.

    Article Title: Establishing broad generality of DNA catalysts for site-specific hydrolysis of single-stranded DNA
    Article Snippet: .. The sample was then brought to 20 µl total volume containing 1× of T4 DNA ligase buffer and one unit of T4 DNA ligase (Fermentas), incubated for 1 h at 37°C, and separated by 8% PAGE. ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Establishing broad generality of DNA catalysts for site-specific hydrolysis of single-stranded DNA
    Article Snippet: .. The sample was then brought to 20 µl total volume containing 1× of T4 DNA ligase buffer and one unit of T4 DNA ligase (Fermentas), incubated for 1 h at 37°C, and separated by 8% PAGE. ..

    Sequencing:

    Article Title: gRNA validation for wheat genome editing with the CRISPR-Cas9 system
    Article Snippet: .. To insert guide sequences into pCR8-U6-NCgRNA, the guide sequence oligos (Additional file ) were first annealed by combining 1 μL of each oligo (100 μM) with 1X T4 DNA ligase buffer (Invitrogen) in a total reaction volume of 10 μL. .. The reaction was heated to 95 °C for 5 min and then left at room temperature for 30 min. Annealed oligos were inserted into pCR8-U6-NCgRNA by simultaneous digestion/ligation using 1 μL annealed oligos, 50 ng pCR8-U6-NCgRNA, 1X NEBuffer 2.1, 2 units BbsI (New England Biolabs), 1X T4 DNA ligase buffer, and 0.5 units T4 DNA ligase (Invitrogen) in a total reaction volume of 10 μL.

    Plasmid Preparation:

    Article Title: Towards the Generation of an ASFV-pA104R DISC Mutant and a Complementary Cell Line—A Potential Methodology for the Production of a Vaccine Candidate
    Article Snippet: .. Since the linearization protocols adopted previously prompted the plasmid and insert with blunt ends, the reaction mixture (20 µL) for this procedure was prepared with 100 ng of linearized pIRESneo, 500 ng of the insert (5:1 ratio), 2 µL of Thermo Scientific 10× T4 DNA Ligase Buffer, 2 µL of 50% PEG 4000 solution, 5 U of T4 DNA Ligase and nuclease-free water. ..

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  • 99
    Thermo Fisher t4 dna ligase hc
    T4 Dna Ligase Hc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 dna ligase hc/product/Thermo Fisher
    Average 99 stars, based on 388 article reviews
    Price from $9.99 to $1999.99
    t4 dna ligase hc - by Bioz Stars, 2020-05
    99/100 stars
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