hbss solution  (Worthington Biochemical)


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    Name:
    HBSS Solution
    Description:
    Sterile calcium and magnesium free Hank s balanced salt solution CMFHBSS pH 7 4 as supplied in the NCIS kit 1 x 500 ml
    Catalog Number:
    LK003210
    Price:
    54
    Size:
    1 ea
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    Structured Review

    Worthington Biochemical hbss solution
    Sterile calcium and magnesium free Hank s balanced salt solution CMFHBSS pH 7 4 as supplied in the NCIS kit 1 x 500 ml
    https://www.bioz.com/result/hbss solution/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbss solution - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Extracellular Tau Oligomers Induce Invasion of Endogenous Tau into the Somatodendritic Compartment and Axonal Transport Dysfunction
    Article Snippet: Briefly, brains were removed from the embryos and placed in Hank’s balanced salt solution (HBSS; Invitrogen) in a sterile environment, after which the cortical tissue was isolated following removal of the meninges. .. The trypsin protease activity was quenched with heat-inactivated fetal bovine serum (FBS; HyClone/GE Healthcare), and the cells were washed 3 times with warm HBSS and treated with 100 units DNAse II (Worthington) per brain. .. The cells were then mechanically dissociated with Pasteur pipettes, diluted in Neurobasal media containing serum-free neuron supplement B27, l-glutamine, glucose, penicillin/streptomycin (all from Invitrogen) and 5% FBS, and plated at roughly 6250 cells/cm2 .

    Incubation:

    Article Title: GRAF1 promotes ferlin-dependent myoblast fusion
    Article Snippet: Once all tissue was harvested, the PBS was exchanged for Hank’s Balanced Salt Solution (HBSS; GIBCO) and tissue was transferred to a sterile 100 mm petri dish for mincing using a sterile razor blade in minimal HBSS. .. Tissue was digested using 0.2% Collagenase, Type II (Worthington Biochem-icals) in HBSS incubated at 37 ° C for 40 min, briefly swirling every 10 min during incubation. .. Digested muscle was triturated 5 times using a wide-mouth pipette and filtered through a 100 μm nylon mesh cell strainer (BD Biosciences).

    Article Title: 5-oxoETE triggers nociception in constipation-predominant irritable bowel syndrome through MAS-related G protein–coupled receptor D
    Article Snippet: DRGs were dissected, minced in HBSS, and incubated in Papain (27 μg/mL) (Sigma, Saint Quentin Fallavier, France) for 20 min at 37°C. .. After a wash with L-15 Wash Buffer [Leibovitz’s L-15 Medium (Invitrogen), 10% FBS (Invitrogen)] and HBSS, the DRGs were incubated in HBSS containing 1 mg/mL of collagenase type IV (Worthington, Lakewood, NJ, USA) and 4 mg/mL of dispase II (Sigma). ..

    Article Title: Loss of ?-Catenin Induces Multifocal Periosteal Chondroma-Like Masses in Mice
    Article Snippet: Chondrocytes were isolated from the epiphyseal cartilage from B6129SF2/J mice (Jackson Laboratory) at day 3 to 5 after birth. .. The epiphyseal cartilage pieces were digested with 0.05% trypsin in HBSS for 1 hour at 37°C and were then incubated with 86 U/mL collagenase type I (Worthington Biochemical Corporation, Lakewood, NJ) in serum-free Dulbecco’s modified Eagle’s medium overnight. .. The dissociated cells were plated on collagen 1 coated wells at the density of 30,000 cells per well in 96-well plates or 50,000 cells per well in 4-well culture slides (BD Biosciences, San Jose, CA) and cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (Gemini Bio-Products, West Sacramento, CA).

    Article Title: 3D Synthetic Peptide-based Architectures for the Engineering of the Enteric Nervous System
    Article Snippet: .. LMMP strips were rinsed twice in HBSS, minced with scissors and dissociated by incubation for 10 min at 37 °C with collagenase type II (1.4 mg/mL, WorthingtonBiochemical Corp, Lakewood, NJ) and DNase (6.25 μg/mL, Calbiochem, Milan, Italy). .. Following enzymatic inactivation, tissues were further triturated using a pipette and collected by centrifugation (350 x g for 8 min).

    Modification:

    Article Title: Loss of ?-Catenin Induces Multifocal Periosteal Chondroma-Like Masses in Mice
    Article Snippet: Chondrocytes were isolated from the epiphyseal cartilage from B6129SF2/J mice (Jackson Laboratory) at day 3 to 5 after birth. .. The epiphyseal cartilage pieces were digested with 0.05% trypsin in HBSS for 1 hour at 37°C and were then incubated with 86 U/mL collagenase type I (Worthington Biochemical Corporation, Lakewood, NJ) in serum-free Dulbecco’s modified Eagle’s medium overnight. .. The dissociated cells were plated on collagen 1 coated wells at the density of 30,000 cells per well in 96-well plates or 50,000 cells per well in 4-well culture slides (BD Biosciences, San Jose, CA) and cultured in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (Gemini Bio-Products, West Sacramento, CA).

    Mouse Assay:

    Article Title: Quantitative Profiling of Peptides from RNAs classified as non-coding
    Article Snippet: .. Mouse cortical cultures Cortices of the mice embryos (C57BL/6, Charles River) at stage E16.5 were dissected and dissociated in 1× Hank’s Balanced Salt Solution (HBSS) (14175-046, Life Technologies), 100 mM MgCl2, 10 mM Kynurenic acid, 100 mM HEPES, 20 mg/ml trypsin (LS003736, Worthington Biochemicals) and 0.32 mg/ml L-cysteine (C7352, Sigma) for 10 min. Trypsin treatment to dissociate cells was terminated with three 2 min washes in 1× HBSS with 10 mg/ml trypsin inhibitor (T6522, Sigma). .. Cells were triturated with a flame-narrowed Pasteur pipette for complete dissociation.

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  • 99
    Worthington Biochemical hbss
    Hbss, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss/product/Worthington Biochemical
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbss - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

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