hbss calcium  (Thermo Fisher)


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    Name:
    HBSS calcium magnesium
    Description:
    Hanks Balanced Salt Solution HBSS is used for a variety of cell culture applications such as washing cells before dissociation transporting cells or tissue diluting cells for counting and preparing reagents Formulations with calcium and magnesium are generally used as transport media or for reagent preparation We offer a variety of Gibco HBSS formulations for a range of cell culture applications Find the right formulation using the media selector tool This HBSS is modified as follows With• Calcium• Magnesium• Phenol Red• Glucose• Sodium BicarbonateThe complete formulation is available Product Intended UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law cGMP Manufacturing and Quality SystemGibco HBSS is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard For supply chain continuity we offer a comparable Gibco HBSS product made in our Grand Island facility 24020 117 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards
    Catalog Number:
    24020083
    Price:
    None
    Applications:
    Cell Culture|Mammalian Cell Culture
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher hbss calcium
    ULK-mediated phosphorylation of ATG14 Ser29 is upregulated under autophagy-inducing conditions. (A) ATG14 Ser29 phosphorylation is induced by nutrient starvation, rapamycin and Torin1. HEK293T cells were incubated in <t>HBSS</t> medium deprived of amino acids or RPMI medium deprived of glucose, or treated with rapamycin (100 nM) or Torin1 (250 nM) in full DMEM medium during the indicated periods of time. The phosphorylation state at Ser29 and the total amount of endogenous ATG14 in cell lysate were analyzed by WB. (B, C) Starvation-induced phosphorylation of ATG14 Ser29 depends on ULK1 and ULK2. MEFs (B) and HCT116 cells (C) with the indicated gene deficiency were incubated in either DMEM (full) or <t>EBSS</t> (starv.) for 30 min. (D) Starvation-induced phosphorylation of ATG14 Ser29 depends on MTORC1. HEK293T cells were stably transduced by the indicated shRNA. The phosphorylation states of ATG14 Ser29 and RPS6KB1 Thr398 and the amounts of the indicated proteins were analyzed by WB. (E) MTORC1-dependent phosphorylation of ATG14 have an effect on ATG14 Ser29 phosphorylation. ATG14 KO HCT116 cells reconstituted with MYC-tagged WT ATG14 or the ATG14[4SA,TA] mutant were incubated with either DMEM or EBSS for 30 min. (F) Quantitative analysis of the effect of the ATG14[4SA,TA] mutation on ATG14 Ser29 phosphorylation. Values are relative to the phosphorylation of WT ATG14 at starvation. Data are represented as mean ± SEM (*, P
    Hanks Balanced Salt Solution HBSS is used for a variety of cell culture applications such as washing cells before dissociation transporting cells or tissue diluting cells for counting and preparing reagents Formulations with calcium and magnesium are generally used as transport media or for reagent preparation We offer a variety of Gibco HBSS formulations for a range of cell culture applications Find the right formulation using the media selector tool This HBSS is modified as follows With• Calcium• Magnesium• Phenol Red• Glucose• Sodium BicarbonateThe complete formulation is available Product Intended UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law cGMP Manufacturing and Quality SystemGibco HBSS is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard For supply chain continuity we offer a comparable Gibco HBSS product made in our Grand Island facility 24020 117 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards
    https://www.bioz.com/result/hbss calcium/product/Thermo Fisher
    Average 99 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    hbss calcium - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14"

    Article Title: The ULK1 complex mediates MTORC1 signaling to the autophagy initiation machinery via binding and phosphorylating ATG14

    Journal: Autophagy

    doi: 10.1080/15548627.2016.1140293

    ULK-mediated phosphorylation of ATG14 Ser29 is upregulated under autophagy-inducing conditions. (A) ATG14 Ser29 phosphorylation is induced by nutrient starvation, rapamycin and Torin1. HEK293T cells were incubated in HBSS medium deprived of amino acids or RPMI medium deprived of glucose, or treated with rapamycin (100 nM) or Torin1 (250 nM) in full DMEM medium during the indicated periods of time. The phosphorylation state at Ser29 and the total amount of endogenous ATG14 in cell lysate were analyzed by WB. (B, C) Starvation-induced phosphorylation of ATG14 Ser29 depends on ULK1 and ULK2. MEFs (B) and HCT116 cells (C) with the indicated gene deficiency were incubated in either DMEM (full) or EBSS (starv.) for 30 min. (D) Starvation-induced phosphorylation of ATG14 Ser29 depends on MTORC1. HEK293T cells were stably transduced by the indicated shRNA. The phosphorylation states of ATG14 Ser29 and RPS6KB1 Thr398 and the amounts of the indicated proteins were analyzed by WB. (E) MTORC1-dependent phosphorylation of ATG14 have an effect on ATG14 Ser29 phosphorylation. ATG14 KO HCT116 cells reconstituted with MYC-tagged WT ATG14 or the ATG14[4SA,TA] mutant were incubated with either DMEM or EBSS for 30 min. (F) Quantitative analysis of the effect of the ATG14[4SA,TA] mutation on ATG14 Ser29 phosphorylation. Values are relative to the phosphorylation of WT ATG14 at starvation. Data are represented as mean ± SEM (*, P
    Figure Legend Snippet: ULK-mediated phosphorylation of ATG14 Ser29 is upregulated under autophagy-inducing conditions. (A) ATG14 Ser29 phosphorylation is induced by nutrient starvation, rapamycin and Torin1. HEK293T cells were incubated in HBSS medium deprived of amino acids or RPMI medium deprived of glucose, or treated with rapamycin (100 nM) or Torin1 (250 nM) in full DMEM medium during the indicated periods of time. The phosphorylation state at Ser29 and the total amount of endogenous ATG14 in cell lysate were analyzed by WB. (B, C) Starvation-induced phosphorylation of ATG14 Ser29 depends on ULK1 and ULK2. MEFs (B) and HCT116 cells (C) with the indicated gene deficiency were incubated in either DMEM (full) or EBSS (starv.) for 30 min. (D) Starvation-induced phosphorylation of ATG14 Ser29 depends on MTORC1. HEK293T cells were stably transduced by the indicated shRNA. The phosphorylation states of ATG14 Ser29 and RPS6KB1 Thr398 and the amounts of the indicated proteins were analyzed by WB. (E) MTORC1-dependent phosphorylation of ATG14 have an effect on ATG14 Ser29 phosphorylation. ATG14 KO HCT116 cells reconstituted with MYC-tagged WT ATG14 or the ATG14[4SA,TA] mutant were incubated with either DMEM or EBSS for 30 min. (F) Quantitative analysis of the effect of the ATG14[4SA,TA] mutation on ATG14 Ser29 phosphorylation. Values are relative to the phosphorylation of WT ATG14 at starvation. Data are represented as mean ± SEM (*, P

    Techniques Used: Incubation, Western Blot, Stable Transfection, shRNA, Mutagenesis

    Related Articles

    Modification:

    Article Title: Influence of Jiegeng on Pharmacokinetic Properties of Flavonoids and Saponins in Gancao
    Article Snippet: .. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), 0.1% EDTA-1.25% trypsin (EDTA/trypsin), penicillin-streptomycin, nonessential Amino Acids and Hank’s Balanced Salt Solution without Calcium & Magnesium (HBSS) were all obtained from Gibco Laboratory (Invitrogen Co, Grand Island, NY, USA). ..

    Isolation:

    Article Title: Immune microenvironment of experimental rat C6 gliomas resembles human glioblastomas
    Article Snippet: .. Immediately after perfusion, brains were isolated and stored in HBSS (without calcium, magnesium) (Invitrogen, Paisley, UK). .. Mechanical and enzymatical tissue dissociation of brain hemispheres was performed with the use of gentle MACS Dissociator, Neural Tissue Dissociation Kit (P) in MACS C Tubes (MACS, Miltenyi Biotec GmbH, Bergish Glabdach, Germany) according to manufacturer’s instructions.

    Incubation:

    Article Title: Complement system activation contributes to the ependymal damage induced by microbial neuraminidase
    Article Snippet: .. Briefly, the explants were sequentially incubated in (1) HBSS without calcium and magnesium (Gibco); (2) TrypLE Express ™ solution (Gibco); (3) αMEM (Gibco) supplemented with 0.2 % Pluronic F-127 (Sigma), 0.3 % D-glucose (Sigma), and 0.01 M HEPES (Sigma; for details of incubation times and temperatures, see [ ]). .. They were then harvested by centrifugation (300×g , 10 min) and resuspended in supplemented αMEM.

    Article Title: Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages
    Article Snippet: .. Flasks were centrifuged at 520 × g for 10 min at 37 °C and subsequently incubated at 37 °C for 30 min. Macrophages were washed 3 times with HBSS containing calcium and magnesium (HBSS+; Gibco) and treated with 100 μg/mL gentamicin (Sigma-Aldrich) in RPMI for 1 h at 37 °C to kill all extracellular bacteria. ..

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    Thermo Fisher hbss calcium magnesium no phenol red
    Hbss Calcium Magnesium No Phenol Red, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss calcium magnesium no phenol red/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hbss calcium magnesium no phenol red - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Thermo Fisher hbss no calcium no magnesium
    Hbss No Calcium No Magnesium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss no calcium no magnesium/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hbss no calcium no magnesium - by Bioz Stars, 2020-09
    99/100 stars
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