hbss 10x no calcium  (Thermo Fisher)


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    Name:
    HBSS 10X no calcium
    Description:
    Hanks Balanced Salt Solution HBSS is used for a variety of cell culture applications such as washing cells before dissociation transporting cells or tissue diluting cells for counting and preparing reagents Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation We offer a variety of Gibco HBSS formulations for a range of cell culture applications Find the right formulation using the media selector tool This HBSS is modified as follows WithWithout• Glucose• Calcium• Phenol Red• Magnesium• Sodium BicarbonateThe complete formulation is available Product Intended UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law cGMP Manufacturing and Quality SystemGibco HBSS is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard Concentrated forms of Gibco HBSS require pH adjustment and filtration at the time of preparation see protocol for details
    Catalog Number:
    14180046
    Price:
    None
    Applications:
    Cell Culture|Mammalian Cell Culture
    Category:
    Cell Culture Transfection Reagents
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    Structured Review

    Thermo Fisher hbss 10x no calcium
    Hanks Balanced Salt Solution HBSS is used for a variety of cell culture applications such as washing cells before dissociation transporting cells or tissue diluting cells for counting and preparing reagents Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation We offer a variety of Gibco HBSS formulations for a range of cell culture applications Find the right formulation using the media selector tool This HBSS is modified as follows WithWithout• Glucose• Calcium• Phenol Red• Magnesium• Sodium BicarbonateThe complete formulation is available Product Intended UseFor in vitro diagnostic use CAUTION Not for human or animal therapeutic use Uses other than the intended use may be a violation of local law cGMP Manufacturing and Quality SystemGibco HBSS is manufactured at a cGMP compliant facility located in Paisley Scotland UK The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard Concentrated forms of Gibco HBSS require pH adjustment and filtration at the time of preparation see protocol for details
    https://www.bioz.com/result/hbss 10x no calcium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbss 10x no calcium - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Molecular mechanism of cardol, isolated from Trigona incisa stingless bee propolis, induced apoptosis in the SW620 human colorectal cancer cell line
    Article Snippet: .. SW620 cells were seeded in each well of 96-well plates at an initial concentration of 8 x 103 cells/well in CM and cultured for 24 h. The cells were then washed twice with 100 μL of 1 x Hank’s buffer salt solution (HBSS, catalog # 14180046, Gibco, Carlsbad, CA) before dihydroethidium (DHE, catalog # D7008, Sigma-Aldrich, St. Louis, MO), dissolved in HBSS to 20 mM, was then added to the incubated cells to a final concentration of 10 mM each and incubated for 30 min. .. The medium was removed, the cells washed twice with 100 μL HBSS and then subjected to cardol treatment at 0 (control), 8 and 14 μg/mL in HBSS at 37 °C for 2 h. The fluorescence was measured at 0, 0.5, 1, 1.5 and 2 h after initiation of the treatment with a microplate reader, at an excitation/emission wavelength of 495/595 nm for DHE.

    Incubation:

    Article Title: Molecular mechanism of cardol, isolated from Trigona incisa stingless bee propolis, induced apoptosis in the SW620 human colorectal cancer cell line
    Article Snippet: .. SW620 cells were seeded in each well of 96-well plates at an initial concentration of 8 x 103 cells/well in CM and cultured for 24 h. The cells were then washed twice with 100 μL of 1 x Hank’s buffer salt solution (HBSS, catalog # 14180046, Gibco, Carlsbad, CA) before dihydroethidium (DHE, catalog # D7008, Sigma-Aldrich, St. Louis, MO), dissolved in HBSS to 20 mM, was then added to the incubated cells to a final concentration of 10 mM each and incubated for 30 min. .. The medium was removed, the cells washed twice with 100 μL HBSS and then subjected to cardol treatment at 0 (control), 8 and 14 μg/mL in HBSS at 37 °C for 2 h. The fluorescence was measured at 0, 0.5, 1, 1.5 and 2 h after initiation of the treatment with a microplate reader, at an excitation/emission wavelength of 495/595 nm for DHE.

    Dissection:

    Article Title: Design, Optimization, and Study of Small Molecules That Target Tau Pre-mRNA and Affect Splicing.
    Article Snippet: .. Approximately 95% of human genes are alternatively spliced, and aberrant splicing events can cause disease. .. Approximately 95% of human genes are alternatively spliced, and aberrant splicing events can cause disease.

    Article Title: Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord
    Article Snippet: .. For neural tube dissection, cervical and thoracic sections of single mouse embryos were dissected in Hanks Balanced Solution without calcium and magnesium (HBSS, Life Technologies, 14185045) supplemented with 5% heat-inactivated foetal bovine serum (FBS). .. The samples were then incubated on FACSmax cell dissociation solution (Amsbio, T200100) with 10× Papain (30 U/mg, Sigma-Aldrich, 10108014001) for 11 min at 37°C to dissociate the cells.

    Article Title: Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division
    Article Snippet: .. In brief, E18.5 embryos were decapitated, and brains were quickly removed and transferred in cold Dissection media [1 x HBSS (Gibco, 14185052), 1 mM sodium pyruvate (Gibco, 11360070), 10 mM HEPES (Gibco, 15630080), 30 mM glucose, 100 U/ml penicillin, and 100 µg/ml streptomycin]. .. Hippocampi were dissected under a binocular microscope and incubated in Dissection medium supplemented with 0.5 mg/ml papain (Worthington, LS003119) and 0.01% DNase (Sigma, DN25) for 20 min at 37°C.

    Cell Culture:

    Article Title: Molecular mechanism of cardol, isolated from Trigona incisa stingless bee propolis, induced apoptosis in the SW620 human colorectal cancer cell line
    Article Snippet: .. SW620 cells were seeded in each well of 96-well plates at an initial concentration of 8 x 103 cells/well in CM and cultured for 24 h. The cells were then washed twice with 100 μL of 1 x Hank’s buffer salt solution (HBSS, catalog # 14180046, Gibco, Carlsbad, CA) before dihydroethidium (DHE, catalog # D7008, Sigma-Aldrich, St. Louis, MO), dissolved in HBSS to 20 mM, was then added to the incubated cells to a final concentration of 10 mM each and incubated for 30 min. .. The medium was removed, the cells washed twice with 100 μL HBSS and then subjected to cardol treatment at 0 (control), 8 and 14 μg/mL in HBSS at 37 °C for 2 h. The fluorescence was measured at 0, 0.5, 1, 1.5 and 2 h after initiation of the treatment with a microplate reader, at an excitation/emission wavelength of 495/595 nm for DHE.

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  • 99
    Thermo Fisher hbss 10x no calcium
    Hbss 10x No Calcium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbss 10x no calcium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hbss 10x no calcium - by Bioz Stars, 2020-09
    99/100 stars
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