haeiii  (TaKaRa)


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    Structured Review

    TaKaRa haeiii
    The <t>CRYBB2</t> mutation cosegregates with the disease in the family. A : Restriction fragment length analysis (RFLP) showing the loss of the <t>HaeIII</t> restriction site in heterozygous individuals with the A2V mutation (185 bp) but it was present in unaffected individuals (121 bp and 64 bp). B : Multiple-sequence alignment in CRYBB2 from different species reveals that codon 2, where the mutation (p. A2V) occurred, is highly conserved (highlighted in red).
    Haeiii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/haeiii/product/TaKaRa
    Average 93 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    haeiii - by Bioz Stars, 2022-11
    93/100 stars

    Images

    1) Product Images from "Characterization of a novel mutation in the CRYBB2 gene associated with autosomal dominant congenital posterior subcapsular cataract in a Chinese family"

    Article Title: Characterization of a novel mutation in the CRYBB2 gene associated with autosomal dominant congenital posterior subcapsular cataract in a Chinese family

    Journal: Molecular Vision

    doi:

    The CRYBB2 mutation cosegregates with the disease in the family. A : Restriction fragment length analysis (RFLP) showing the loss of the HaeIII restriction site in heterozygous individuals with the A2V mutation (185 bp) but it was present in unaffected individuals (121 bp and 64 bp). B : Multiple-sequence alignment in CRYBB2 from different species reveals that codon 2, where the mutation (p. A2V) occurred, is highly conserved (highlighted in red).
    Figure Legend Snippet: The CRYBB2 mutation cosegregates with the disease in the family. A : Restriction fragment length analysis (RFLP) showing the loss of the HaeIII restriction site in heterozygous individuals with the A2V mutation (185 bp) but it was present in unaffected individuals (121 bp and 64 bp). B : Multiple-sequence alignment in CRYBB2 from different species reveals that codon 2, where the mutation (p. A2V) occurred, is highly conserved (highlighted in red).

    Techniques Used: Mutagenesis, Sequencing

    2) Product Images from "Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV)"

    Article Title: Ligation-mediated amplification for effective rapid determination of viral RNA sequences (RDV)

    Journal: Journal of Clinical Virology

    doi: 10.1016/j.jcv.2008.05.004

    The design of adaptors and primer sets used in RDV ver3.0. (A) Adaptor-Sse83871 contains sticky-end structures digested with Sau3AI, whereas Adaptor-NotI contains blunt-end structures digested with HaeIII. (B) All primers used in RDV ver3.0. Bold letters indicate 2-variable nucleotides.
    Figure Legend Snippet: The design of adaptors and primer sets used in RDV ver3.0. (A) Adaptor-Sse83871 contains sticky-end structures digested with Sau3AI, whereas Adaptor-NotI contains blunt-end structures digested with HaeIII. (B) All primers used in RDV ver3.0. Bold letters indicate 2-variable nucleotides.

    Techniques Used:

    Location of viral cDNA fragments on YOKV genome amplified using the RDV ver3.0. Column A shows 10 cDNA fragments expected to be detected in RDV ver3.0 (over 150 bp cDNA fegments digested by Sau3AI and HaeIII). Columns B–E show YOKV cDNA fragments detected in RDV ver3.0. Column B shows 8 of 10 cDNA fragments as shown in A. Column C shows cDNA fragments partially digested with HaeIII or Sau3AI. These cDNA fragments contained undigested sequences by the restriction enzymes. Column D shows amplicons detected as 2 or 3 ligated cDNA fragments. Column E shows cDNA fragments not containing recognition sequences of restriction enzymes at the ends.
    Figure Legend Snippet: Location of viral cDNA fragments on YOKV genome amplified using the RDV ver3.0. Column A shows 10 cDNA fragments expected to be detected in RDV ver3.0 (over 150 bp cDNA fegments digested by Sau3AI and HaeIII). Columns B–E show YOKV cDNA fragments detected in RDV ver3.0. Column B shows 8 of 10 cDNA fragments as shown in A. Column C shows cDNA fragments partially digested with HaeIII or Sau3AI. These cDNA fragments contained undigested sequences by the restriction enzymes. Column D shows amplicons detected as 2 or 3 ligated cDNA fragments. Column E shows cDNA fragments not containing recognition sequences of restriction enzymes at the ends.

    Techniques Used: Amplification

    3) Product Images from "Yeast Community Structures and Dynamics in Healthy and Botrytis-Affected Grape Must Fermentations ▿"

    Article Title: Yeast Community Structures and Dynamics in Healthy and Botrytis-Affected Grape Must Fermentations ▿

    Journal:

    doi: 10.1128/AEM.01279-07

    Representative restriction patterns of the 5.8S ITS region of yeast isolates obtained with HaeIII (lanes 1 to 7) or DdeI (lanes 8 to 17). Lanes: M, 100-bp molecular marker; 1, C. lusitaniae ; 2 and 3, C. zemplinina ; 4, Kazachstania sp.; 5, L. thermotolerans
    Figure Legend Snippet: Representative restriction patterns of the 5.8S ITS region of yeast isolates obtained with HaeIII (lanes 1 to 7) or DdeI (lanes 8 to 17). Lanes: M, 100-bp molecular marker; 1, C. lusitaniae ; 2 and 3, C. zemplinina ; 4, Kazachstania sp.; 5, L. thermotolerans

    Techniques Used: Marker

    4) Product Images from "Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex-PCR-Based Restriction Analysis and Direct Sequencing ▿"

    Article Title: Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex-PCR-Based Restriction Analysis and Direct Sequencing ▿

    Journal:

    doi: 10.1128/JCM.01214-06

    Identification of NTM reference strains by HaeIII digestion of 515-bp duplex PCR amplicons. Lanes: M, 50-bp ladder; 1, M. avium (ATCC 25291); 2, M. intracellulare ; 3, M. scrofulaceum ; 4, M. kansasii type I; 5, M. kansasii type II; 6, M. szulgai ; 7, M.
    Figure Legend Snippet: Identification of NTM reference strains by HaeIII digestion of 515-bp duplex PCR amplicons. Lanes: M, 50-bp ladder; 1, M. avium (ATCC 25291); 2, M. intracellulare ; 3, M. scrofulaceum ; 4, M. kansasii type I; 5, M. kansasii type II; 6, M. szulgai ; 7, M.

    Techniques Used: Polymerase Chain Reaction

    Algorithm for species differentiation by duplex PRA with AvaII and HaeIII. This algorithm was based on the hsp65 sequences of 54 mycobacterial reference strains previously reported. After completing duplex PCR which can differentiate NTM strains and
    Figure Legend Snippet: Algorithm for species differentiation by duplex PRA with AvaII and HaeIII. This algorithm was based on the hsp65 sequences of 54 mycobacterial reference strains previously reported. After completing duplex PCR which can differentiate NTM strains and

    Techniques Used: Polymerase Chain Reaction

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