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Zorka h2 o2
H2 O2, supplied by Zorka, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2 o2/product/Zorka
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
h2 o2 - by Bioz Stars, 2021-03
86/100 stars

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Irradiation:

Article Title: EVI1 carboxy-terminal phosphorylation is ATM-mediated and sustains transcriptional modulation and self-renewal via enhanced CtBP1 association
Article Snippet: .. Genotoxic stress was induced by treatment with H2 O2 at concentrations previously used , or irradiation using a 320 kV X-ray system (Gulmay Medical Ltd) for suspension culture leukemia cells or using the image-guided micro-irradiation system for 96 well plates provided via the SARRP research platform (Xstrahl). .. Induction of activation of the DNA damage response was confirmed by demonstration of increased quantities of γH2AX foci and p53 phosphorylation.

Incubation:

Article Title: iRhom2 loss alleviates renal injury in long-term PM2.5-exposed mice by suppression of inflammation and oxidative stress
Article Snippet: IHC analysis for phosphorylated JNK (ab131499, 1:200, Abcam, USA), phosphorylated NF-κB (ab86299, 1:200, Abcam) and TACE (ab2051, 1:200, Abcam) were performed as previously described . .. In brief, renal tissue sections were incubated in 3% H2 O2 to block endogenous peroxidase activity for 10 min 5% bovine serum albumin (BSA, Shanghai Boao Biotechnology Co., Ltd., Shanghai, China) was used for blocking non-specific binding for 1 h. Then, tissues were incubated with primary antibodies. ..

Article Title: ZNF692 promotes colon adenocarcinoma cell growth and metastasis by activating the PI3K/AKT pathway
Article Snippet: Immunohistochemistry (IHC) Briefly, formalin-fixed and paraffin-embedded tumor tissues were cut into 4 µ m-thick sections. .. The sections were deparaffinized by xylene, rehydrated in a graded series of ethanol, incubated with 3% H2 O2 for 30 min, blocked with 3% bovine serum albumin for 1 h, and incubated with an anti-Ki-67 antibody (polyclonal, rabbit anti-human IgG; 1:50; cat. no. WL0280a; Wanleibio Co., Ltd., Shenyang, China) at 4°C overnight. .. The next day, the tissue sections were incubated with a secondary antibody (biotin-labeled goat anti-rabbit IgG; 1:200; cat. no. A0277; Beyotime Institute of Biotechnology, Shanghai, China) and streptavidin-HRP (HRP-labeled streptavidin; 1:200; cat. no. A0303; Beyotime Institute of Biotechnology) at room temperature for 30 min. Color was developed using a 3,3′-diaminobenzidine (DAB) Substrate kit (cat. no. DA1010; Beijing Solarbio Science and Technology Co., Ltd., Beijing, China).

Blocking Assay:

Article Title: iRhom2 loss alleviates renal injury in long-term PM2.5-exposed mice by suppression of inflammation and oxidative stress
Article Snippet: IHC analysis for phosphorylated JNK (ab131499, 1:200, Abcam, USA), phosphorylated NF-κB (ab86299, 1:200, Abcam) and TACE (ab2051, 1:200, Abcam) were performed as previously described . .. In brief, renal tissue sections were incubated in 3% H2 O2 to block endogenous peroxidase activity for 10 min 5% bovine serum albumin (BSA, Shanghai Boao Biotechnology Co., Ltd., Shanghai, China) was used for blocking non-specific binding for 1 h. Then, tissues were incubated with primary antibodies. ..

Activity Assay:

Article Title: iRhom2 loss alleviates renal injury in long-term PM2.5-exposed mice by suppression of inflammation and oxidative stress
Article Snippet: IHC analysis for phosphorylated JNK (ab131499, 1:200, Abcam, USA), phosphorylated NF-κB (ab86299, 1:200, Abcam) and TACE (ab2051, 1:200, Abcam) were performed as previously described . .. In brief, renal tissue sections were incubated in 3% H2 O2 to block endogenous peroxidase activity for 10 min 5% bovine serum albumin (BSA, Shanghai Boao Biotechnology Co., Ltd., Shanghai, China) was used for blocking non-specific binding for 1 h. Then, tissues were incubated with primary antibodies. ..

Binding Assay:

Article Title: iRhom2 loss alleviates renal injury in long-term PM2.5-exposed mice by suppression of inflammation and oxidative stress
Article Snippet: IHC analysis for phosphorylated JNK (ab131499, 1:200, Abcam, USA), phosphorylated NF-κB (ab86299, 1:200, Abcam) and TACE (ab2051, 1:200, Abcam) were performed as previously described . .. In brief, renal tissue sections were incubated in 3% H2 O2 to block endogenous peroxidase activity for 10 min 5% bovine serum albumin (BSA, Shanghai Boao Biotechnology Co., Ltd., Shanghai, China) was used for blocking non-specific binding for 1 h. Then, tissues were incubated with primary antibodies. ..

Concentration Assay:

Article Title: Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract
Article Snippet: SB431542 [TGF-β RI inhibitor, (HY-10431, MedChemExpress, New Jersey, USA)] and LY2109761 [TGF-β RI/II inhibitor, (HY-12075, MedChemExpress)] were dissolved in DMSO to prepare a 20 mM and 10 mM stock solution, respectively. .. SB431542 and LY2109761 were applied to cells (final concentration, 10 µM and 5 µM, respectively) which had been exposed to 400 µM H2 O2 for 24 h. TGF-β1 RP (APA124Hu01, USCN Business Co., Ltd., Wuhan, China) was dissolved in PBS to prepare a 10 µg/ml stock solution and applied to cells at 15 ng/ml for 96 h. SB203580 (S8307-1MG, Sigma Aldrich; Merck KGaA, Darmstadt, Germany), a cytokine-suppressive anti-inflammatory drug, was used as a p38 MAPK inhibitor in this study. ..

other:

Article Title: Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells
Article Snippet: In the presence of dihydroquercetin at time points 15, 30, 45, and 60 min, there was significant attenuation of H2 O2 -induced damage in all tested time points, but the control group also displayed significant attenuation of damaged cells in the tested time course.

Article Title: Reversible Oxidation of Myometrial Voltage-Gated Potassium Channels with Hydrogen Peroxide
Article Snippet: Reagents The following reagents were used: H2 O2 (ZORKA Pharma, Sabac, Serbia); 4-AP (Sigma Chemical Co, St Louis, MO, USA).

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    Zorka h2 o2
    H2 O2, supplied by Zorka, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Zorka
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    86/100 stars
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