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FUJIFILM h2 o2
H2 O2, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2 o2/product/FUJIFILM
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
h2 o2 - by Bioz Stars, 2021-03
86/100 stars

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Immunohistochemistry:

Article Title: CD4+ Resident Memory T Cells Mediate Long-Term Local Skin Immune Memory of Contact Hypersensitivity in BALB/c Mice
Article Snippet: .. In immunohistochemistry (IHC), the fixed sections were incubated in 0.36% H2 O2 in methanol (30 min) to block endogenous peroxidase, with 20% goat serum (FUJIFILM Wako) in block ace (DS Pharma Promo, Osaka, Japan) for blocking (100 min), then with primary mAbs (5 μg/ml, 120 min). .. Primary rat mAbs were purified anti-mouse CD3ε (17A2), CD4 (RM4-5), CD8α (53-6.7) + CD8β (H35-17.2), and Gr-1 (RB6-8C5) (Tonbo Biosciences).

Incubation:

Article Title: CD4+ Resident Memory T Cells Mediate Long-Term Local Skin Immune Memory of Contact Hypersensitivity in BALB/c Mice
Article Snippet: .. In immunohistochemistry (IHC), the fixed sections were incubated in 0.36% H2 O2 in methanol (30 min) to block endogenous peroxidase, with 20% goat serum (FUJIFILM Wako) in block ace (DS Pharma Promo, Osaka, Japan) for blocking (100 min), then with primary mAbs (5 μg/ml, 120 min). .. Primary rat mAbs were purified anti-mouse CD3ε (17A2), CD4 (RM4-5), CD8α (53-6.7) + CD8β (H35-17.2), and Gr-1 (RB6-8C5) (Tonbo Biosciences).

Article Title: Spheroid cancer stem cells display reprogrammed metabolism and obtain energy by actively running the tricarboxylic acid (TCA) cycle
Article Snippet: The ALDH enzymatic activity of OVTOKO and SiHa cells (1×106 cells) was measured as described [ ], using the ALDEFLUOR kit (STEMCELL Technologies, Canada). .. Intracellular reactive oxygen species (ROS) assays Dissotiated single cells were incubated with 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA, Sigma-Aldrich, USA) at 37°C for 40 min. After washing, cells were exposed to 100 mM H2 O2 (Wako) for 20 min. Fluorescence intensity was measured using a FACS Calibur flow cytometer. .. Metabolite measurements Metabolome analysis was carried out through a facility service(C-SCOPE) at Human Metabolome Technologies, Inc.(HMT, Inc., Japan) as previously described (n=3 in each group) [ , ].

Blocking Assay:

Article Title: CD4+ Resident Memory T Cells Mediate Long-Term Local Skin Immune Memory of Contact Hypersensitivity in BALB/c Mice
Article Snippet: .. In immunohistochemistry (IHC), the fixed sections were incubated in 0.36% H2 O2 in methanol (30 min) to block endogenous peroxidase, with 20% goat serum (FUJIFILM Wako) in block ace (DS Pharma Promo, Osaka, Japan) for blocking (100 min), then with primary mAbs (5 μg/ml, 120 min). .. Primary rat mAbs were purified anti-mouse CD3ε (17A2), CD4 (RM4-5), CD8α (53-6.7) + CD8β (H35-17.2), and Gr-1 (RB6-8C5) (Tonbo Biosciences).

Article Title: Preventive Effects of Tryptophan–Methionine Dipeptide on Neural Inflammation and Alzheimer’s Pathology
Article Snippet: .. Then, the sections were treated with blocking solution (8% w /v skimmed milk) for 30 min after inactivation of endogenous peroxidase with 3% H2 O2 (Wako) in methanol for 5 min. .. The sections were then incubated overnight at room temperature with either a monoclonal anti-human Aβx–42 antibody (12F4, Millipore, Billerica, MA, USA) or a polyclonal anti-Iba-1 antibody (Wako) as primary antibodies.

other:

Article Title: Microbicidal action of photoirradiated aqueous extracts from wine lees
Article Snippet: Reagents were purchased from the following sources: 5,5-dimethyl-1-pyrroline N -oxide (DMPO) from Labotec (Tokyo, Japan); catalase from bovine liver, H2 O2 , dimethyl sulfoxide (DMSO), thiourea, and l -ascorbic acid from Wako Pure Chemical Industries (Osaka, Japan); 4-hydroxy-2,2,6,6-tetramethylpiperidine N -oxyl (TEMPOL) from Sigma-Aldrich (St. Louis, MO, USA); and 2,2-diphenyl-1-picrylhydrazyl (DPPH) from Tokyo Chemical Industry (Tokyo, Japan).

Article Title: Effects of HF on the Lithiation Behavior of the Silicon Anode in LiPF6 Organic Electrolyte Solution
Article Snippet: 4.1 Materials H2 SO4 (96–98%), H2 O2 (30–35%), HCl (35%), and HF (46%) aqueous solutions and anhydrous PC solvent ( > 98%, battery grade) were purchased from Wako Pure Chemicals.

Fluorescence:

Article Title: Spheroid cancer stem cells display reprogrammed metabolism and obtain energy by actively running the tricarboxylic acid (TCA) cycle
Article Snippet: The ALDH enzymatic activity of OVTOKO and SiHa cells (1×106 cells) was measured as described [ ], using the ALDEFLUOR kit (STEMCELL Technologies, Canada). .. Intracellular reactive oxygen species (ROS) assays Dissotiated single cells were incubated with 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA, Sigma-Aldrich, USA) at 37°C for 40 min. After washing, cells were exposed to 100 mM H2 O2 (Wako) for 20 min. Fluorescence intensity was measured using a FACS Calibur flow cytometer. .. Metabolite measurements Metabolome analysis was carried out through a facility service(C-SCOPE) at Human Metabolome Technologies, Inc.(HMT, Inc., Japan) as previously described (n=3 in each group) [ , ].

Article Title: A Peroxiredoxin From the Haemaphysalis longicornis Tick Affects Langat Virus Replication in a Hamster Cell Line
Article Snippet: The cells were observed under a fluorescent microscope (IX71, Olympus, Tokyo, Japan). .. Furthermore, to measure the fluorescence intensities of H2 O2 in the cells, the cells were collected using 120 μl of 0.25% Trypsin-EDTA solution (Wako Pure Chemical Industries, Ltd.). ..

FACS:

Article Title: Spheroid cancer stem cells display reprogrammed metabolism and obtain energy by actively running the tricarboxylic acid (TCA) cycle
Article Snippet: The ALDH enzymatic activity of OVTOKO and SiHa cells (1×106 cells) was measured as described [ ], using the ALDEFLUOR kit (STEMCELL Technologies, Canada). .. Intracellular reactive oxygen species (ROS) assays Dissotiated single cells were incubated with 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA, Sigma-Aldrich, USA) at 37°C for 40 min. After washing, cells were exposed to 100 mM H2 O2 (Wako) for 20 min. Fluorescence intensity was measured using a FACS Calibur flow cytometer. .. Metabolite measurements Metabolome analysis was carried out through a facility service(C-SCOPE) at Human Metabolome Technologies, Inc.(HMT, Inc., Japan) as previously described (n=3 in each group) [ , ].

Flow Cytometry:

Article Title: Spheroid cancer stem cells display reprogrammed metabolism and obtain energy by actively running the tricarboxylic acid (TCA) cycle
Article Snippet: The ALDH enzymatic activity of OVTOKO and SiHa cells (1×106 cells) was measured as described [ ], using the ALDEFLUOR kit (STEMCELL Technologies, Canada). .. Intracellular reactive oxygen species (ROS) assays Dissotiated single cells were incubated with 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA, Sigma-Aldrich, USA) at 37°C for 40 min. After washing, cells were exposed to 100 mM H2 O2 (Wako) for 20 min. Fluorescence intensity was measured using a FACS Calibur flow cytometer. .. Metabolite measurements Metabolome analysis was carried out through a facility service(C-SCOPE) at Human Metabolome Technologies, Inc.(HMT, Inc., Japan) as previously described (n=3 in each group) [ , ].

Cytometry:

Article Title: Spheroid cancer stem cells display reprogrammed metabolism and obtain energy by actively running the tricarboxylic acid (TCA) cycle
Article Snippet: The ALDH enzymatic activity of OVTOKO and SiHa cells (1×106 cells) was measured as described [ ], using the ALDEFLUOR kit (STEMCELL Technologies, Canada). .. Intracellular reactive oxygen species (ROS) assays Dissotiated single cells were incubated with 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA, Sigma-Aldrich, USA) at 37°C for 40 min. After washing, cells were exposed to 100 mM H2 O2 (Wako) for 20 min. Fluorescence intensity was measured using a FACS Calibur flow cytometer. .. Metabolite measurements Metabolome analysis was carried out through a facility service(C-SCOPE) at Human Metabolome Technologies, Inc.(HMT, Inc., Japan) as previously described (n=3 in each group) [ , ].

Synthesized:

Article Title: Enhancing Visible Light Absorption of Yellow-Colored Peroxo-Titanate Nanotubes Prepared Using Peroxo Titanium Complex Ions
Article Snippet: Yellow TNT Preparation As a precursor, the peroxo titanium complex ion ([Ti(OH)3 O2 ]− ) was synthesized by the following methods of the previous study: initially, 0.63 g of TiH2 ( > 99%, Kojundo Chemical Laboratory Co., Ltd., Saitama, Japan) was dissolved in 62.46 mL of mixed solution with pH 10 containing H2 O2 (30%, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and NaOH (97%, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) aqueous solution for 2 h at 10 °C. .. Yellow TNT Preparation As a precursor, the peroxo titanium complex ion ([Ti(OH)3 O2 ]− ) was synthesized by the following methods of the previous study: initially, 0.63 g of TiH2 ( > 99%, Kojundo Chemical Laboratory Co., Ltd., Saitama, Japan) was dissolved in 62.46 mL of mixed solution with pH 10 containing H2 O2 (30%, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and NaOH (97%, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) aqueous solution for 2 h at 10 °C. ..

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    FUJIFILM h2 o2
    H2 O2, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/FUJIFILM
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    FUJIFILM hydrogen peroxide solution
    Hydrogen Peroxide Solution, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydrogen peroxide solution/product/FUJIFILM
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hydrogen peroxide solution - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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