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Biogenex h2 o2
H2 O2, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h2 o2/product/Biogenex
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
h2 o2 - by Bioz Stars, 2021-03
86/100 stars

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Related Articles

Activity Assay:

Article Title: PTEN Regulates PDGF Ligand Switch for ?-PDGFR Signaling in Prostate Cancer
Article Snippet: Slides of formalin-fixed, paraffin-embedded prostate sections from PTEN+/+ and PTEN−/− mice at the age of 8 weeks were deparaffinized with xylene, then rehydrated sequentially with decreasing concentrations of EtOH from 100% to 70%, followed by water. .. Endogenous peroxidase activity was blocked with 3% H2 O2 , and antigen retrieval was performed by steaming for 20 minutes in Antigen Retrieval Citra Plus Solution (BioGenex, Freemont, CA). .. Slides were then washed twice with PBS and blocked with Cas-Block solution (Invitrogen).

Article Title: ERG/AKR1C3/AR constitutes a feed-forward loop for AR signaling in prostate cancer cells
Article Snippet: Formalin-fixed, paraffin-embedded serial tissue sections from human prostate tumor tissue specimens and LuCaP xenograft tumors were deparaffinized with xylene and rehydrated in graded EtOH. .. Endogenous peroxidase activity was blocked by incubating in 3% H2 O2 for 20 min. Antigen retrieval was performed with Antigen Retrieval Citra Plus Solution (BioGenex, Freemont, CA) in a steamer. .. Slides were then blocked with Blocking Serum from ABC Vectastain Kit (Vector Labs, Burlingame, CA).

Incubation:

Article Title: Low levels of tissue factor lead to alveolar hemorrhage, potentiating murine acute lung injury and oxidative stress
Article Snippet: .. Slides were rinsed with DI water and incubated for 20 minutes in 0.3% H2 O2 in cold methanol then rinsed and blocked (Powerblock, Biogenex, Fremont, CA and 0.4% Triton) for 15 minutes in a humid environment. .. Slides were rinsed and incubated overnight at 4°C with 1:200 goat polyclonal anti-TF Ab (R & D Systems, Minneapolis, MN), rinsed, and incubated with a biotinylated rabbit anti-goat secondary antibody (Innogenex, San Ramon, CA) for 20 minutes at room temperature.

Article Title: Synergistic effects of glycated chitosan with high-intensity focused ultrasound on suppression of metastases in a syngeneic breast tumor model
Article Snippet: .. The sections were subsequently blocked with 3% H2 O2 and protein blocking reagent (Biogenex Laboratories, San Ramon, CA, USA) in the dark and then incubated with anti-mouse F4/80 antibody (eBioscience Inc., San Diego, CA, USA) for 2 h, followed by horseradish peroxidase (HRP)-conjugated secondary antibody for 1 h. Finally, tissue sections were incubated with Liquid DAB+Substrate Chromogen System (Dako North America Inc., Carpinteria, VA, USA) until a brown color developed, and then counterstained with hematoxylin. .. The slides were visualized under light microscope, entire tissue sections were examined, and three random regions were selected and photographed using a digital camera.

Article Title: Inhibition of Ca2+-Independent Phospholipase A2 Results in Insufficient Insulin Secretion and Impaired Glucose Tolerance
Article Snippet: Isolated mouse pancreases were fixed overnight in 10% formalin at 4 C. Sections (4- μ m) were cut using ThermoShandon Finesse Microtome (Thermo Shandon, Inc., Pittsburgh, PA). .. Slides were incubated in 4% H2 O2 to block the endogenous peroxidase, and then Powerblock universal blocking reagent (10X, BioGenex Laboratories, Inc., San Ramon CA) was used to eliminate the background. .. Insulin, glucagon, and iPLA2 were stained with the rabbit antiinsulin antibodies (Santa Cruz Biotechnology), rabbit antiglucagon antibodies (DAKO Corp., Carpinteria, CA), or goat anti-iPLA2 antibodies (Santa Cruz Biotechnology), respectively.

Blocking Assay:

Article Title: Synergistic effects of glycated chitosan with high-intensity focused ultrasound on suppression of metastases in a syngeneic breast tumor model
Article Snippet: .. The sections were subsequently blocked with 3% H2 O2 and protein blocking reagent (Biogenex Laboratories, San Ramon, CA, USA) in the dark and then incubated with anti-mouse F4/80 antibody (eBioscience Inc., San Diego, CA, USA) for 2 h, followed by horseradish peroxidase (HRP)-conjugated secondary antibody for 1 h. Finally, tissue sections were incubated with Liquid DAB+Substrate Chromogen System (Dako North America Inc., Carpinteria, VA, USA) until a brown color developed, and then counterstained with hematoxylin. .. The slides were visualized under light microscope, entire tissue sections were examined, and three random regions were selected and photographed using a digital camera.

Article Title: Inhibition of Ca2+-Independent Phospholipase A2 Results in Insufficient Insulin Secretion and Impaired Glucose Tolerance
Article Snippet: Isolated mouse pancreases were fixed overnight in 10% formalin at 4 C. Sections (4- μ m) were cut using ThermoShandon Finesse Microtome (Thermo Shandon, Inc., Pittsburgh, PA). .. Slides were incubated in 4% H2 O2 to block the endogenous peroxidase, and then Powerblock universal blocking reagent (10X, BioGenex Laboratories, Inc., San Ramon CA) was used to eliminate the background. .. Insulin, glucagon, and iPLA2 were stained with the rabbit antiinsulin antibodies (Santa Cruz Biotechnology), rabbit antiglucagon antibodies (DAKO Corp., Carpinteria, CA), or goat anti-iPLA2 antibodies (Santa Cruz Biotechnology), respectively.

Article Title: CXCR4, but not CXCR7, discriminates metastatic behavior in non-small cell lung cancer cells
Article Snippet: Immunohistochemistry of factor VIII-related antigen, Ki67 and GFP were performed as previously described ( , ). .. Briefly, paraffin-embedded tumor sections were de-paraffinized, subjected to antigen retrieval (HK080-9K, Biogenex, San Ramos, CA), fixed for 20min in 1:1 absolute methanol and 3% H2 O2 , rinsed in PBS, and then blocked (HK085-5K, Biogenex) at room temperature for 30 min followed by Avidin/Biotin blocking (SP-2001, Vector Laboratories, Burlingame, CA). .. Then, a 1:800 dilution of anti-factor VIII related antibody, anti-Ki67 Ab-4 rabbit polyclonal antibody (RB-1510 Thermo Scientific) or control rabbit IgG or goat anti-GFP(Vector labs SP-0702) or control goat IgG (Sigma, I5356) were added and slides were incubated for 30 min.

Article Title: Down-regulation of transforming growth factor-? type II receptor (TGF-?RII) protein and mRNA expression in cervical cancer
Article Snippet: Following deparaffination, the sections were immersed in an antigen-retrieval solution (DAKO, Glostrup, Denmark) for 40 min at 98°C. .. Endogen peroxidase was blocked with 3% H2 O2 in absolute methanol, followed by immersion in a universal blocking reagent (Powerblock, Biogenex, San Ramon, CA, USA) for 10 min. .. The sections were incubated overnight at room temperature with rabbit polyclonal antibodies against TGF-β2 or TGF-βRII (Santa Cruz Laboratory, Santa Cruz, CA, USA) diluted 1/50 in PBS.

Avidin-Biotin Assay:

Article Title: CXCR4, but not CXCR7, discriminates metastatic behavior in non-small cell lung cancer cells
Article Snippet: Immunohistochemistry of factor VIII-related antigen, Ki67 and GFP were performed as previously described ( , ). .. Briefly, paraffin-embedded tumor sections were de-paraffinized, subjected to antigen retrieval (HK080-9K, Biogenex, San Ramos, CA), fixed for 20min in 1:1 absolute methanol and 3% H2 O2 , rinsed in PBS, and then blocked (HK085-5K, Biogenex) at room temperature for 30 min followed by Avidin/Biotin blocking (SP-2001, Vector Laboratories, Burlingame, CA). .. Then, a 1:800 dilution of anti-factor VIII related antibody, anti-Ki67 Ab-4 rabbit polyclonal antibody (RB-1510 Thermo Scientific) or control rabbit IgG or goat anti-GFP(Vector labs SP-0702) or control goat IgG (Sigma, I5356) were added and slides were incubated for 30 min.

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    Biogenex h2 o2
    H2 O2, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Biogenex endogenous peroxidase activity
    Endogenous Peroxidase Activity, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase activity/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2021-03
    86/100 stars
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    86
    Biogenex 3 3 diaminobenzidine h2 o2
    3 3 Diaminobenzidine H2 O2, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine h2 o2/product/Biogenex
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 3 diaminobenzidine h2 o2 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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