h2 o2  (Biocare Medical)

 
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    Structured Review

    Biocare Medical h2 o2
    H2 O2, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Biocare Medical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Inactivation of the tumor suppressor gene von Hippel-Lindau (VHL) in granulocytes contributes to development of liver hemangiomas in a mouse model
    Article Snippet: 4 μm thick paraffin sections were stained with hematoxylin and eosin (H & E) according to standard procedures. .. For immunohistochemistry, 4 μm thick paraffin sections were dewaxed, and heat mediated antigen retrieval was performed with citrate buffer, pH 6, for 40 min. Endogenous peroxidase was quenched by incubating sections for 15 min in methanol with 0.3 % H2 O2 or peroxidase block (Peroxidased 1, Biocare Medical, Concord, CA). .. Endogenous biotin was blocked with avidin-biotin blocking kit (Vector Laboratories, Burlingame, CA, USA), followed by 30 min blocking with 3 % or 10 % (GFP stain) normal goat serum (Sigma-Aldrich, St. Louis, MO) in PBS.

    Article Title: Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial–Mesenchymal Transition, and Kidney Tubular Injury in Mice
    Article Snippet: GAPDH expression was used to normalize the expression. .. Immunohistochemical Staining of Collagen I and E-Cadherin The paraffin slides were deparaffinized, heated in citrate buffer for 15 minutes, incubated in 3% H2 O2 in PBS for endogenous peroxidase inhibition, and incubated in blocking solution (Biocare Medical, Cat No. STHRP700H). .. Thereafter, the slides were incubated with rabbit anti-E-cadherin antibody (Abcam, Cat. No. ab76319, 1:300 dilution) and Rabbit anti-collagen I (Abcam, Cat. No. ab34710, 1:300 dilution) overnight in a humidity chamber at 4°C.

    Blocking Assay:

    Article Title: Inactivation of the tumor suppressor gene von Hippel-Lindau (VHL) in granulocytes contributes to development of liver hemangiomas in a mouse model
    Article Snippet: 4 μm thick paraffin sections were stained with hematoxylin and eosin (H & E) according to standard procedures. .. For immunohistochemistry, 4 μm thick paraffin sections were dewaxed, and heat mediated antigen retrieval was performed with citrate buffer, pH 6, for 40 min. Endogenous peroxidase was quenched by incubating sections for 15 min in methanol with 0.3 % H2 O2 or peroxidase block (Peroxidased 1, Biocare Medical, Concord, CA). .. Endogenous biotin was blocked with avidin-biotin blocking kit (Vector Laboratories, Burlingame, CA, USA), followed by 30 min blocking with 3 % or 10 % (GFP stain) normal goat serum (Sigma-Aldrich, St. Louis, MO) in PBS.

    Article Title: TP53-inducible Glycolysis and Apoptosis Regulator (TIGAR) Metabolically Reprograms Carcinoma and Stromal Cells in Breast Cancer *
    Article Snippet: .. Antigen retrieval was performed in 0.01 m citrate buffer, pH 6.0, for 10 min using a pressure cooker followed by blocking for endogenous peroxidase in 3% H2 O2 for 15 min. An avidin-biotin kit (Biocare Medical) was used to block endogenous biotin. .. For BCL2 rabbit antibody (ab 182858, Abcam), sections were blocked with 10% goat serum and incubated with antibody for 1 h at room temperature.

    Article Title: EPH/ephrin profile and EPHB2 expression predicts patient survival in breast cancer
    Article Snippet: .. A washing buffer, consisting in TBS-0.1% BSA, was used previous to inactivation of endogenous peroxidase in 3% H2 O2 for 10 min. Unspecific binding was blocked with serum-free protein block (Background Sniper, Biocare Medical) for 10 min in a moisture chamber. .. The rabbit anti-EPHB2 antibody (1:300) was incubated overnight at 4°C.

    Article Title: Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial–Mesenchymal Transition, and Kidney Tubular Injury in Mice
    Article Snippet: GAPDH expression was used to normalize the expression. .. Immunohistochemical Staining of Collagen I and E-Cadherin The paraffin slides were deparaffinized, heated in citrate buffer for 15 minutes, incubated in 3% H2 O2 in PBS for endogenous peroxidase inhibition, and incubated in blocking solution (Biocare Medical, Cat No. STHRP700H). .. Thereafter, the slides were incubated with rabbit anti-E-cadherin antibody (Abcam, Cat. No. ab76319, 1:300 dilution) and Rabbit anti-collagen I (Abcam, Cat. No. ab34710, 1:300 dilution) overnight in a humidity chamber at 4°C.

    Article Title: Phylogeny and expression of carbonic anhydrase-related proteins
    Article Snippet: .. The sections were immunohistochemically stained according to the following procedure: (i) incubating the sample in methanol + 3% H2 O2 for 5 min; (ii) rinsing with 1 × Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween; (iii) blocking with Rodent Block M™(Biocare Medical, Concord, CA) for 30 min; (iv) incubating with primary rabbit anti-human CARP VIII, CARP X, and CARP XI antibodies (Santa Cruz Biotechnology, Inc. Bergheimer Heidelberg, Germany) raised against amino acids 1 to 100 (CARP VIII), 1 to 50 (CARP X) and 279 to 328 (CARP XI) diluted 1:350 in 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) for 1 hour at room temperature and rinsing with TBS containing 0.05% Tween. .. Notably, rabbit IgG was used as a control instead of the primary antibodies; (v) incubation with Rabbit HRP-Polymer + XM Factor™(Biocare Medical, Concord, CA) (2.5 ml HRP-Polymer + 1 to 2 drops XM Factor) for 30 min prior to rinsing with 1 × TBS containing 0.05% Tween; (vi) treatment with 1 × 3,3'-diaminobenzidine tetrahydrochloride (DAB) solution for 5 min prior to rinsing with distilled water; (vii) counterstaining of the slides with Mayer's Hematoxylin for 1 to 3 sec and rinsing under tap water for 10 min. After dehydration, the slides were mounted with Entellan Neu™ (Merck; Darmstadt, Germany), examined and photographed using Nikon Microphot microscope (Nikon Microphot-FXA, Japan).

    Activity Assay:

    Article Title: ESR1 and PGR polymorphisms are associated with estrogen and progesterone receptor expression in breast tumors
    Article Snippet: For assessment of ERα and PgR expression using the AQUA system, sections of TMA were subjected to double immunofluorescence staining as previously described ( ). .. In brief, after deparaffinization and rehydration, TMA slides were subjected to microwave epitope retrieval, then endogenous peroxidase activity was blocked with 2.5% (vol/vol) H2 O2 in methanol for 30 min. Nonspecific binding of the antibodies was extinguished by a 30 min incubation with “Background Sniper” (BioCare Medical, Concord, CA). .. The TMA slide was then incubated with the tumor-specific antibody, wide-spectrum cytokeratin (DAKO, Rabbit polyclonal antibody, Z-0622 1:250) overnight at 4°C.

    Binding Assay:

    Article Title: ESR1 and PGR polymorphisms are associated with estrogen and progesterone receptor expression in breast tumors
    Article Snippet: For assessment of ERα and PgR expression using the AQUA system, sections of TMA were subjected to double immunofluorescence staining as previously described ( ). .. In brief, after deparaffinization and rehydration, TMA slides were subjected to microwave epitope retrieval, then endogenous peroxidase activity was blocked with 2.5% (vol/vol) H2 O2 in methanol for 30 min. Nonspecific binding of the antibodies was extinguished by a 30 min incubation with “Background Sniper” (BioCare Medical, Concord, CA). .. The TMA slide was then incubated with the tumor-specific antibody, wide-spectrum cytokeratin (DAKO, Rabbit polyclonal antibody, Z-0622 1:250) overnight at 4°C.

    Article Title: EPH/ephrin profile and EPHB2 expression predicts patient survival in breast cancer
    Article Snippet: .. A washing buffer, consisting in TBS-0.1% BSA, was used previous to inactivation of endogenous peroxidase in 3% H2 O2 for 10 min. Unspecific binding was blocked with serum-free protein block (Background Sniper, Biocare Medical) for 10 min in a moisture chamber. .. The rabbit anti-EPHB2 antibody (1:300) was incubated overnight at 4°C.

    Article Title: The Desmoplastic Stroma Plays an Essential Role in the Accumulation and Modulation of Infiltrated Immune Cells in Pancreatic Adenocarcinoma
    Article Snippet: .. Endogenous peroxidase was minimized by 10 minutes incubation in H2 O2 , and nonspecific binding was avoided by incubating with Background Sniper (Biocare Medical) or 1% bovine serum albumin for 10 min. .. The samples were immunostained overnight with antibodies (Ab) for CXCL8 (1 : 25, Becton Dickinson), COX-2 (1 : 200, CRM306B, Biocare Medical), S100 (dilution 1 : 1000, Z 0311, Dako, Sweden), CD163 (dilution 1 : 10, ab74604), CD83 (dilution 1 : 50, ab64875), CD8 (dilution 1 : 600, ab4055), IL-1α (1 : 40, ab7632), CCL2 (1 : 1000, ab73680), and CCL20 (1 : 40, ab9829) (Abcam, UK).

    Incubation:

    Article Title: ESR1 and PGR polymorphisms are associated with estrogen and progesterone receptor expression in breast tumors
    Article Snippet: For assessment of ERα and PgR expression using the AQUA system, sections of TMA were subjected to double immunofluorescence staining as previously described ( ). .. In brief, after deparaffinization and rehydration, TMA slides were subjected to microwave epitope retrieval, then endogenous peroxidase activity was blocked with 2.5% (vol/vol) H2 O2 in methanol for 30 min. Nonspecific binding of the antibodies was extinguished by a 30 min incubation with “Background Sniper” (BioCare Medical, Concord, CA). .. The TMA slide was then incubated with the tumor-specific antibody, wide-spectrum cytokeratin (DAKO, Rabbit polyclonal antibody, Z-0622 1:250) overnight at 4°C.

    Article Title: The Desmoplastic Stroma Plays an Essential Role in the Accumulation and Modulation of Infiltrated Immune Cells in Pancreatic Adenocarcinoma
    Article Snippet: .. Endogenous peroxidase was minimized by 10 minutes incubation in H2 O2 , and nonspecific binding was avoided by incubating with Background Sniper (Biocare Medical) or 1% bovine serum albumin for 10 min. .. The samples were immunostained overnight with antibodies (Ab) for CXCL8 (1 : 25, Becton Dickinson), COX-2 (1 : 200, CRM306B, Biocare Medical), S100 (dilution 1 : 1000, Z 0311, Dako, Sweden), CD163 (dilution 1 : 10, ab74604), CD83 (dilution 1 : 50, ab64875), CD8 (dilution 1 : 600, ab4055), IL-1α (1 : 40, ab7632), CCL2 (1 : 1000, ab73680), and CCL20 (1 : 40, ab9829) (Abcam, UK).

    Article Title: Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial–Mesenchymal Transition, and Kidney Tubular Injury in Mice
    Article Snippet: GAPDH expression was used to normalize the expression. .. Immunohistochemical Staining of Collagen I and E-Cadherin The paraffin slides were deparaffinized, heated in citrate buffer for 15 minutes, incubated in 3% H2 O2 in PBS for endogenous peroxidase inhibition, and incubated in blocking solution (Biocare Medical, Cat No. STHRP700H). .. Thereafter, the slides were incubated with rabbit anti-E-cadherin antibody (Abcam, Cat. No. ab76319, 1:300 dilution) and Rabbit anti-collagen I (Abcam, Cat. No. ab34710, 1:300 dilution) overnight in a humidity chamber at 4°C.

    Avidin-Biotin Assay:

    Article Title: TP53-inducible Glycolysis and Apoptosis Regulator (TIGAR) Metabolically Reprograms Carcinoma and Stromal Cells in Breast Cancer *
    Article Snippet: .. Antigen retrieval was performed in 0.01 m citrate buffer, pH 6.0, for 10 min using a pressure cooker followed by blocking for endogenous peroxidase in 3% H2 O2 for 15 min. An avidin-biotin kit (Biocare Medical) was used to block endogenous biotin. .. For BCL2 rabbit antibody (ab 182858, Abcam), sections were blocked with 10% goat serum and incubated with antibody for 1 h at room temperature.

    Staining:

    Article Title: Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial–Mesenchymal Transition, and Kidney Tubular Injury in Mice
    Article Snippet: GAPDH expression was used to normalize the expression. .. Immunohistochemical Staining of Collagen I and E-Cadherin The paraffin slides were deparaffinized, heated in citrate buffer for 15 minutes, incubated in 3% H2 O2 in PBS for endogenous peroxidase inhibition, and incubated in blocking solution (Biocare Medical, Cat No. STHRP700H). .. Thereafter, the slides were incubated with rabbit anti-E-cadherin antibody (Abcam, Cat. No. ab76319, 1:300 dilution) and Rabbit anti-collagen I (Abcam, Cat. No. ab34710, 1:300 dilution) overnight in a humidity chamber at 4°C.

    Article Title: Phylogeny and expression of carbonic anhydrase-related proteins
    Article Snippet: .. The sections were immunohistochemically stained according to the following procedure: (i) incubating the sample in methanol + 3% H2 O2 for 5 min; (ii) rinsing with 1 × Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween; (iii) blocking with Rodent Block M™(Biocare Medical, Concord, CA) for 30 min; (iv) incubating with primary rabbit anti-human CARP VIII, CARP X, and CARP XI antibodies (Santa Cruz Biotechnology, Inc. Bergheimer Heidelberg, Germany) raised against amino acids 1 to 100 (CARP VIII), 1 to 50 (CARP X) and 279 to 328 (CARP XI) diluted 1:350 in 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) for 1 hour at room temperature and rinsing with TBS containing 0.05% Tween. .. Notably, rabbit IgG was used as a control instead of the primary antibodies; (v) incubation with Rabbit HRP-Polymer + XM Factor™(Biocare Medical, Concord, CA) (2.5 ml HRP-Polymer + 1 to 2 drops XM Factor) for 30 min prior to rinsing with 1 × TBS containing 0.05% Tween; (vi) treatment with 1 × 3,3'-diaminobenzidine tetrahydrochloride (DAB) solution for 5 min prior to rinsing with distilled water; (vii) counterstaining of the slides with Mayer's Hematoxylin for 1 to 3 sec and rinsing under tap water for 10 min. After dehydration, the slides were mounted with Entellan Neu™ (Merck; Darmstadt, Germany), examined and photographed using Nikon Microphot microscope (Nikon Microphot-FXA, Japan).

    Inhibition:

    Article Title: Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial–Mesenchymal Transition, and Kidney Tubular Injury in Mice
    Article Snippet: GAPDH expression was used to normalize the expression. .. Immunohistochemical Staining of Collagen I and E-Cadherin The paraffin slides were deparaffinized, heated in citrate buffer for 15 minutes, incubated in 3% H2 O2 in PBS for endogenous peroxidase inhibition, and incubated in blocking solution (Biocare Medical, Cat No. STHRP700H). .. Thereafter, the slides were incubated with rabbit anti-E-cadherin antibody (Abcam, Cat. No. ab76319, 1:300 dilution) and Rabbit anti-collagen I (Abcam, Cat. No. ab34710, 1:300 dilution) overnight in a humidity chamber at 4°C.

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  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • h2 o2  (Biocare Medical)
    86
    Biocare Medical h2 o2
    H2 O2, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h2 o2/product/Biocare Medical
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h2 o2 - by Bioz Stars, 2021-03
    86/100 stars
    		  Buy from Supplier

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