Structured Review

GE Healthcare h damgo
H Damgo, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/h damgo/product/GE Healthcare
Average 99 stars, based on 10 article reviews
Price from $9.99 to $1999.99
h damgo - by Bioz Stars, 2020-09
99/100 stars

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Radio Immunoprecipitation:

Article Title: Lactic acid bacterium, Lactobacillus paracasei KW3110, suppresses inflammatory stress-induced caspase-1 activation by promoting interleukin-10 production in mouse and human immune cells
Article Snippet: .. The cells were then primed with 10 μg/mL of LPS for 4 h, and stimulated with 2 mM ATP for 1 h. After stimulation, cells were lysed with radioimmunoprecipitation assay buffer and whole cell lysates were prepared and then boiled in sodium dodecyl sulfate (SDS) sample buffer for 5 min at 95 ˚C. ..

Modification:

Article Title: Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase
Article Snippet: .. PhastSystem 20% gels, buffer strips, silver staining reagents and the PhastSystem were also from Amersham Pharmacia Biotech. λ DNA and restriction, modification and PCR enzymes were purchased from either Gibco BRL, Promega, New England Biolabs or Stratagene. .. S -adenosylmethionine (AdoMet) and S -adenosylhomocysteine (AdoHcy) were purchased from Sigma and purified ( ).

Incubation:

Article Title: Muscleblind-like 1 interacts with RNA hairpins in splicing target and pathogenic RNAs
Article Snippet: .. After three washes (10 ml each) of buffer (WB) containing 25 mM Tris–Cl, pH 8.0, 300 mM NaCl, 5 mM β-mercaptoethanol, 0.1% Triton X-100, the glutathione-Sepharose beads were incubated with 4 ml WB containing 40 U of PreScission protease (Amersham) at 4°C overnight. .. Chemical and enzymatic analysis of RNA structures Transcription reactions were carried out in a 50 µl volume which contained 2 μg of each DNA template, 1 mM rNTPs, 3.3 mM guanosine, 60 U of ribonuclease inhibitor RNase Out (Invitrogen), 200 U of T7 RNA polymerase (Ambion, Austin, TX, USA), 10 mM DTT, 40 mM Tris-HCl (pH 7.9), 6 mM MgCl2 , 2 mM spermidine, 10 mM NaCl.

Polymerase Chain Reaction:

Article Title: Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase
Article Snippet: .. PhastSystem 20% gels, buffer strips, silver staining reagents and the PhastSystem were also from Amersham Pharmacia Biotech. λ DNA and restriction, modification and PCR enzymes were purchased from either Gibco BRL, Promega, New England Biolabs or Stratagene. .. S -adenosylmethionine (AdoMet) and S -adenosylhomocysteine (AdoHcy) were purchased from Sigma and purified ( ).

Western Blot:

Article Title: Muscleblind-like 1 interacts with RNA hairpins in splicing target and pathogenic RNAs
Article Snippet: .. After three washes (10 ml each) of buffer (WB) containing 25 mM Tris–Cl, pH 8.0, 300 mM NaCl, 5 mM β-mercaptoethanol, 0.1% Triton X-100, the glutathione-Sepharose beads were incubated with 4 ml WB containing 40 U of PreScission protease (Amersham) at 4°C overnight. .. Chemical and enzymatic analysis of RNA structures Transcription reactions were carried out in a 50 µl volume which contained 2 μg of each DNA template, 1 mM rNTPs, 3.3 mM guanosine, 60 U of ribonuclease inhibitor RNase Out (Invitrogen), 200 U of T7 RNA polymerase (Ambion, Austin, TX, USA), 10 mM DTT, 40 mM Tris-HCl (pH 7.9), 6 mM MgCl2 , 2 mM spermidine, 10 mM NaCl.

Silver Staining:

Article Title: Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase
Article Snippet: .. PhastSystem 20% gels, buffer strips, silver staining reagents and the PhastSystem were also from Amersham Pharmacia Biotech. λ DNA and restriction, modification and PCR enzymes were purchased from either Gibco BRL, Promega, New England Biolabs or Stratagene. .. S -adenosylmethionine (AdoMet) and S -adenosylhomocysteine (AdoHcy) were purchased from Sigma and purified ( ).

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    GE Healthcare h damgo
    H Damgo, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h damgo/product/GE Healthcare
    Average 89 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    h damgo - by Bioz Stars, 2020-09
    89/100 stars
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