guinea pig anti hcn1  (Alomone Labs)


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    Alomone Labs guinea pig anti hcn1
    Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with <t>HCN1</t> (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.
    Guinea Pig Anti Hcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti hcn1/product/Alomone Labs
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    guinea pig anti hcn1 - by Bioz Stars, 2022-08
    90/100 stars

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    1) Product Images from "miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels"

    Article Title: miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels

    Journal: Pain

    doi: 10.1097/j.pain.0000000000000971

    Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.
    Figure Legend Snippet: Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.

    Techniques Used: Expressing, In Vivo, Mouse Assay

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    Alomone Labs hcn1
    TRIP8b interaction with <t>HCN1</t> is independent of TRIP8b alternative splicing and is bipartite
    Hcn1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcn1/product/Alomone Labs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcn1 - by Bioz Stars, 2022-08
    90/100 stars
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    TRIP8b interaction with HCN1 is independent of TRIP8b alternative splicing and is bipartite

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Alternatively spliced isoforms of TRIP8b differentially control h channel trafficking and function

    doi: 10.1523/JNEUROSCI.0856-09.2009

    Figure Lengend Snippet: TRIP8b interaction with HCN1 is independent of TRIP8b alternative splicing and is bipartite

    Article Snippet: The following primary antibodies were used: mouse (ms) monoclonal antibodies to α-tubulin (DM1A, Upstate Biotechnology, Lake Placid, NY); rabbit (rab) polyclonal antibodies to HCN1 (Alomone Labs, Jerusalem); ms monoclonal to HA epitope (F-7, Santa Cruz Biotechnology, Santa Cruz, CA); Guinea pig (gp) polyclonal antibodies to HCN1 ( ); gp polyclonal antibodies to HCN2 ( ); rab and gp polyclonal antibodies to green fluorescent protein (see antibody generation and , available at as ); rab polyclonal antibodies to TRIP8b ( ); gp polyclonal antibodies to TRIP8b (see antibody generation and , available at as ); gp polyclonal antibodies to HCN4 ( ); gp polyclonal antibodies to TRIP8b exon 1a-5, exon 2, and exon 4 (see antibody generation and ); ms monoclonal to MAP-2 (Sigma, St. Louis, MO).

    Techniques:

    TRIP8b isoforms increase or decrease I h peak current in HCN1 cotransfected HEK293T cells depending on isoform identity

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Alternatively spliced isoforms of TRIP8b differentially control h channel trafficking and function

    doi: 10.1523/JNEUROSCI.0856-09.2009

    Figure Lengend Snippet: TRIP8b isoforms increase or decrease I h peak current in HCN1 cotransfected HEK293T cells depending on isoform identity

    Article Snippet: The following primary antibodies were used: mouse (ms) monoclonal antibodies to α-tubulin (DM1A, Upstate Biotechnology, Lake Placid, NY); rabbit (rab) polyclonal antibodies to HCN1 (Alomone Labs, Jerusalem); ms monoclonal to HA epitope (F-7, Santa Cruz Biotechnology, Santa Cruz, CA); Guinea pig (gp) polyclonal antibodies to HCN1 ( ); gp polyclonal antibodies to HCN2 ( ); rab and gp polyclonal antibodies to green fluorescent protein (see antibody generation and , available at as ); rab polyclonal antibodies to TRIP8b ( ); gp polyclonal antibodies to TRIP8b (see antibody generation and , available at as ); gp polyclonal antibodies to HCN4 ( ); gp polyclonal antibodies to TRIP8b exon 1a-5, exon 2, and exon 4 (see antibody generation and ); ms monoclonal to MAP-2 (Sigma, St. Louis, MO).

    Techniques:

    TRIP8b isoforms bidirectionally modify HCN1 protein surface expression as determined by flow cytometry

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Alternatively spliced isoforms of TRIP8b differentially control h channel trafficking and function

    doi: 10.1523/JNEUROSCI.0856-09.2009

    Figure Lengend Snippet: TRIP8b isoforms bidirectionally modify HCN1 protein surface expression as determined by flow cytometry

    Article Snippet: The following primary antibodies were used: mouse (ms) monoclonal antibodies to α-tubulin (DM1A, Upstate Biotechnology, Lake Placid, NY); rabbit (rab) polyclonal antibodies to HCN1 (Alomone Labs, Jerusalem); ms monoclonal to HA epitope (F-7, Santa Cruz Biotechnology, Santa Cruz, CA); Guinea pig (gp) polyclonal antibodies to HCN1 ( ); gp polyclonal antibodies to HCN2 ( ); rab and gp polyclonal antibodies to green fluorescent protein (see antibody generation and , available at as ); rab polyclonal antibodies to TRIP8b ( ); gp polyclonal antibodies to TRIP8b (see antibody generation and , available at as ); gp polyclonal antibodies to HCN4 ( ); gp polyclonal antibodies to TRIP8b exon 1a-5, exon 2, and exon 4 (see antibody generation and ); ms monoclonal to MAP-2 (Sigma, St. Louis, MO).

    Techniques: Expressing, Flow Cytometry

    TRIP8b isoforms A4, B2, and B3 alter amount of surface HCN1 protein

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Alternatively spliced isoforms of TRIP8b differentially control h channel trafficking and function

    doi: 10.1523/JNEUROSCI.0856-09.2009

    Figure Lengend Snippet: TRIP8b isoforms A4, B2, and B3 alter amount of surface HCN1 protein

    Article Snippet: The following primary antibodies were used: mouse (ms) monoclonal antibodies to α-tubulin (DM1A, Upstate Biotechnology, Lake Placid, NY); rabbit (rab) polyclonal antibodies to HCN1 (Alomone Labs, Jerusalem); ms monoclonal to HA epitope (F-7, Santa Cruz Biotechnology, Santa Cruz, CA); Guinea pig (gp) polyclonal antibodies to HCN1 ( ); gp polyclonal antibodies to HCN2 ( ); rab and gp polyclonal antibodies to green fluorescent protein (see antibody generation and , available at as ); rab polyclonal antibodies to TRIP8b ( ); gp polyclonal antibodies to TRIP8b (see antibody generation and , available at as ); gp polyclonal antibodies to HCN4 ( ); gp polyclonal antibodies to TRIP8b exon 1a-5, exon 2, and exon 4 (see antibody generation and ); ms monoclonal to MAP-2 (Sigma, St. Louis, MO).

    Techniques:

    Isoforms of TRIP8b alter the trafficking of HCN1 in cultured hippocampal neurons

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Alternatively spliced isoforms of TRIP8b differentially control h channel trafficking and function

    doi: 10.1523/JNEUROSCI.0856-09.2009

    Figure Lengend Snippet: Isoforms of TRIP8b alter the trafficking of HCN1 in cultured hippocampal neurons

    Article Snippet: The following primary antibodies were used: mouse (ms) monoclonal antibodies to α-tubulin (DM1A, Upstate Biotechnology, Lake Placid, NY); rabbit (rab) polyclonal antibodies to HCN1 (Alomone Labs, Jerusalem); ms monoclonal to HA epitope (F-7, Santa Cruz Biotechnology, Santa Cruz, CA); Guinea pig (gp) polyclonal antibodies to HCN1 ( ); gp polyclonal antibodies to HCN2 ( ); rab and gp polyclonal antibodies to green fluorescent protein (see antibody generation and , available at as ); rab polyclonal antibodies to TRIP8b ( ); gp polyclonal antibodies to TRIP8b (see antibody generation and , available at as ); gp polyclonal antibodies to HCN4 ( ); gp polyclonal antibodies to TRIP8b exon 1a-5, exon 2, and exon 4 (see antibody generation and ); ms monoclonal to MAP-2 (Sigma, St. Louis, MO).

    Techniques: Cell Culture

    Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.

    Journal: Pain

    Article Title: miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels

    doi: 10.1097/j.pain.0000000000000971

    Figure Lengend Snippet: Analyses of expression of the Cav2.3 protein in neuronal and nonneuronal cells or in cell membrane of the cells in dorsal root ganglia in vivo. Representative images to demonstrate expression of Cav2.3 in mouse DRG and colabeling with PGP9.5-positive neuronal cells (A), GFAP-positive satellite cells (B) and with HCN1 (hyperpolarization-activated cyclic nucleotide-gated) channel in the cell membrane (C). Observed colocalization is highlighted with white arrows. Quantification of coexpression of each neuronal subtype with the Cav2.3 expressing neurons is shown in panel D. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.

    Article Snippet: Primary antibodies used for IF are Guinea pig anti-PGP9.5 (1:100 dilution, 14104, Neuromics, Edina, MN), Guinea pig anti-HCN1 (1:100, Alomone Labs, AGP203) rabbit anti-Cav2.3 antibody (1:80, Alomone Labs, ACC-006), Biotinylated-Isolectin B4 (1:100; B-1205, Vector, Burlingame, CA), Guinea pig Substance P (1:150; Neuromics GP14103), Anti-GFAP (1:500; NeuroMab clone N206A/8, UC Davis, Davis, CA) and Chicken anti-NF200 (1:500; Neuromics CH23015).

    Techniques: Expressing, In Vivo, Mouse Assay