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Promega gtp rnasin
Gtp Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gtp rnasin/product/Promega
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
gtp rnasin - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Hybridization Histochemistry of Neural Transcripts
Article Snippet: .. Linearized plasmid or PCR product containing cDNA to be transcribed Labeled nucleotide (select one): [α−35 S]UTP ( > 1000 Ci/mmol; Perkin-Elmer) 1 mM UTP/4 mM digoxigenin-11-UTP mix (ingredients from Roche; store mix indefinitely at –20°C) 5× transcription buffer (see recipe) 100 mM and 5 M dithiothreitol (DTT) 10 mM individual solutions of ATP, CTP, and GTP RNasin (Promega) 10 to 20 U/µl RNA polymerase (SP6, T3, or T7) 1 U/µl RNase-free DNase I ( APPENDIX 2A ) TE buffer, pH 7.6 ( APPENDIX 2A ) 4 M NaCl 25 µg/µl yeast tRNA 70% and 100% (v/v) ethanol 10% (w/v) sodium dodecyl sulfate (SDS) TE buffer/5% (w/v) SDS NOTE: Use DEPC-treated water ( APPENDIX 2A ) for all reagents. .. For radiolabeled probes : Combine ~250 ng linearized plasmid (or 10 to 100 ng PCR product) with [35 S]UTP at a final concentration of 20 to 30 µM in a microcentrifuge tube.

Concentration Assay:

Article Title: Hybridization Histochemistry of Neural Transcripts
Article Snippet: Linearized plasmid or PCR product containing cDNA to be transcribed Labeled nucleotide (select one): [α−35 S]UTP ( > 1000 Ci/mmol; Perkin-Elmer) 1 mM UTP/4 mM digoxigenin-11-UTP mix (ingredients from Roche; store mix indefinitely at –20°C) 5× transcription buffer (see recipe) 100 mM and 5 M dithiothreitol (DTT) 10 mM individual solutions of ATP, CTP, and GTP RNasin (Promega) 10 to 20 U/µl RNA polymerase (SP6, T3, or T7) 1 U/µl RNase-free DNase I ( APPENDIX 2A ) TE buffer, pH 7.6 ( APPENDIX 2A ) 4 M NaCl 25 µg/µl yeast tRNA 70% and 100% (v/v) ethanol 10% (w/v) sodium dodecyl sulfate (SDS) TE buffer/5% (w/v) SDS NOTE: Use DEPC-treated water ( APPENDIX 2A ) for all reagents. .. For radiolabeled probes : Combine ~250 ng linearized plasmid (or 10 to 100 ng PCR product) with [35 S]UTP at a final concentration of 20 to 30 µM in a microcentrifuge tube.

Northern Blot:

Article Title: Hybridization Histochemistry of Neural Transcripts
Article Snippet: Linearized plasmid or PCR product containing cDNA to be transcribed Labeled nucleotide (select one): [α−35 S]UTP ( > 1000 Ci/mmol; Perkin-Elmer) 1 mM UTP/4 mM digoxigenin-11-UTP mix (ingredients from Roche; store mix indefinitely at –20°C) 5× transcription buffer (see recipe) 100 mM and 5 M dithiothreitol (DTT) 10 mM individual solutions of ATP, CTP, and GTP RNasin (Promega) 10 to 20 U/µl RNA polymerase (SP6, T3, or T7) 1 U/µl RNase-free DNase I ( APPENDIX 2A ) TE buffer, pH 7.6 ( APPENDIX 2A ) 4 M NaCl 25 µg/µl yeast tRNA 70% and 100% (v/v) ethanol 10% (w/v) sodium dodecyl sulfate (SDS) TE buffer/5% (w/v) SDS NOTE: Use DEPC-treated water ( APPENDIX 2A ) for all reagents. .. When making probes for northern analysis, [32 P]dATP can be substituted for [35 S]dATP.

Plasmid Preparation:

Article Title: Hybridization Histochemistry of Neural Transcripts
Article Snippet: .. Linearized plasmid or PCR product containing cDNA to be transcribed Labeled nucleotide (select one): [α−35 S]UTP ( > 1000 Ci/mmol; Perkin-Elmer) 1 mM UTP/4 mM digoxigenin-11-UTP mix (ingredients from Roche; store mix indefinitely at –20°C) 5× transcription buffer (see recipe) 100 mM and 5 M dithiothreitol (DTT) 10 mM individual solutions of ATP, CTP, and GTP RNasin (Promega) 10 to 20 U/µl RNA polymerase (SP6, T3, or T7) 1 U/µl RNase-free DNase I ( APPENDIX 2A ) TE buffer, pH 7.6 ( APPENDIX 2A ) 4 M NaCl 25 µg/µl yeast tRNA 70% and 100% (v/v) ethanol 10% (w/v) sodium dodecyl sulfate (SDS) TE buffer/5% (w/v) SDS NOTE: Use DEPC-treated water ( APPENDIX 2A ) for all reagents. .. For radiolabeled probes : Combine ~250 ng linearized plasmid (or 10 to 100 ng PCR product) with [35 S]UTP at a final concentration of 20 to 30 µM in a microcentrifuge tube.

Article Title: Hybridization Histochemistry of Neural Transcripts
Article Snippet: .. Linearized plasmid containing cDNA to be transcribed [α-35 S]UTP ( > 1000 Ci/mmol; Perkin-Elmer) 5× transcription buffer (see recipe) 100 mM dithiothreitol (DTT) 10 mM ATP, CTP, and GTP RNasin (Promega) 10 to 20 U/μl RNA polymerase (SP6, T3, or T7) 1 mM UTP/4 mM digoxigenin-11-UTP mix (ingredients available from Roche; store mix indefinitely at −20°C) 1 U/μl RNase-free DNase I ( APPENDIX 2A ) TE buffer ( APPENDIX 2A ) 4 M NaCl 25 μg/μl yeast tRNA 70% and 100% ethanol 5 M DTT 10% (w/v) sodium dodecyl sulfate (SDS) TE buffer/5% (w/v) SDS NOTE: Use DEPC-treated water ( APPENDIX 2A ) for all reagents. .. 1a Combine ~250 ng of linearized plasmid (or 10 to 100 ng of PCR product) with [35 S]UTP at a final concentration of 20 to 30 μM in a microcentrifuge tube.

Labeling:

Article Title: Hybridization Histochemistry of Neural Transcripts
Article Snippet: .. Linearized plasmid or PCR product containing cDNA to be transcribed Labeled nucleotide (select one): [α−35 S]UTP ( > 1000 Ci/mmol; Perkin-Elmer) 1 mM UTP/4 mM digoxigenin-11-UTP mix (ingredients from Roche; store mix indefinitely at –20°C) 5× transcription buffer (see recipe) 100 mM and 5 M dithiothreitol (DTT) 10 mM individual solutions of ATP, CTP, and GTP RNasin (Promega) 10 to 20 U/µl RNA polymerase (SP6, T3, or T7) 1 U/µl RNase-free DNase I ( APPENDIX 2A ) TE buffer, pH 7.6 ( APPENDIX 2A ) 4 M NaCl 25 µg/µl yeast tRNA 70% and 100% (v/v) ethanol 10% (w/v) sodium dodecyl sulfate (SDS) TE buffer/5% (w/v) SDS NOTE: Use DEPC-treated water ( APPENDIX 2A ) for all reagents. .. For radiolabeled probes : Combine ~250 ng linearized plasmid (or 10 to 100 ng PCR product) with [35 S]UTP at a final concentration of 20 to 30 µM in a microcentrifuge tube.

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    Promega gtp rnasin
    Gtp Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gtp rnasin/product/Promega
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gtp rnasin - by Bioz Stars, 2020-04
    93/100 stars
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