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Melatonin alters oxidative balance during both normoxic and hypoxic gestations. Representative photographs ( A ) and protein levels of catalase (CAT; ( B )), glutathione peroxidase 1/2 <t>(GPX1/2;</t> ( C )), superoxide dismutase-1 (SOD1; ( D )), superoxide dismutase-2 (SOD2; ( E )) and hemeoxigenase-1 (HO-1; ( F )) in left ventricle from newborn guinea pigs exposed to gestational normoxia (N; red) or hypoxia (H; blue) and treated with vehicle or melatonin during gestation. In panel ( A ), the separated band corresponds to the loading control (LC), which was used to normalize all blots based on densitometry. Values are expressed as mean ± SEM. n = 5–6 independent samples (one per pup) per group. Data were analyzed using two-way ANOVA with FDR post hoc test. Significant differences ( q ≤ 0.05): * vs. normoxic-counterpart, # vs. vehicle-counterpart.
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Melatonin alters oxidative balance during both normoxic and hypoxic gestations. Representative photographs ( A ) and protein levels of catalase (CAT; ( B )), glutathione peroxidase 1/2 <t>(GPX1/2;</t> ( C )), superoxide dismutase-1 (SOD1; ( D )), superoxide dismutase-2 (SOD2; ( E )) and hemeoxigenase-1 (HO-1; ( F )) in left ventricle from newborn guinea pigs exposed to gestational normoxia (N; red) or hypoxia (H; blue) and treated with vehicle or melatonin during gestation. In panel ( A ), the separated band corresponds to the loading control (LC), which was used to normalize all blots based on densitometry. Values are expressed as mean ± SEM. n = 5–6 independent samples (one per pup) per group. Data were analyzed using two-way ANOVA with FDR post hoc test. Significant differences ( q ≤ 0.05): * vs. normoxic-counterpart, # vs. vehicle-counterpart.
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Melatonin alters oxidative balance during both normoxic and hypoxic gestations. Representative photographs ( A ) and protein levels of catalase (CAT; ( B )), glutathione peroxidase 1/2 <t>(GPX1/2;</t> ( C )), superoxide dismutase-1 (SOD1; ( D )), superoxide dismutase-2 (SOD2; ( E )) and hemeoxigenase-1 (HO-1; ( F )) in left ventricle from newborn guinea pigs exposed to gestational normoxia (N; red) or hypoxia (H; blue) and treated with vehicle or melatonin during gestation. In panel ( A ), the separated band corresponds to the loading control (LC), which was used to normalize all blots based on densitometry. Values are expressed as mean ± SEM. n = 5–6 independent samples (one per pup) per group. Data were analyzed using two-way ANOVA with FDR post hoc test. Significant differences ( q ≤ 0.05): * vs. normoxic-counterpart, # vs. vehicle-counterpart.
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Melatonin alters oxidative balance during both normoxic and hypoxic gestations. Representative photographs ( A ) and protein levels of catalase (CAT; ( B )), glutathione peroxidase 1/2 <t>(GPX1/2;</t> ( C )), superoxide dismutase-1 (SOD1; ( D )), superoxide dismutase-2 (SOD2; ( E )) and hemeoxigenase-1 (HO-1; ( F )) in left ventricle from newborn guinea pigs exposed to gestational normoxia (N; red) or hypoxia (H; blue) and treated with vehicle or melatonin during gestation. In panel ( A ), the separated band corresponds to the loading control (LC), which was used to normalize all blots based on densitometry. Values are expressed as mean ± SEM. n = 5–6 independent samples (one per pup) per group. Data were analyzed using two-way ANOVA with FDR post hoc test. Significant differences ( q ≤ 0.05): * vs. normoxic-counterpart, # vs. vehicle-counterpart.
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Melatonin alters oxidative balance during both normoxic and hypoxic gestations. Representative photographs ( A ) and protein levels of catalase (CAT; ( B )), glutathione peroxidase 1/2 <t>(GPX1/2;</t> ( C )), superoxide dismutase-1 (SOD1; ( D )), superoxide dismutase-2 (SOD2; ( E )) and hemeoxigenase-1 (HO-1; ( F )) in left ventricle from newborn guinea pigs exposed to gestational normoxia (N; red) or hypoxia (H; blue) and treated with vehicle or melatonin during gestation. In panel ( A ), the separated band corresponds to the loading control (LC), which was used to normalize all blots based on densitometry. Values are expressed as mean ± SEM. n = 5–6 independent samples (one per pup) per group. Data were analyzed using two-way ANOVA with FDR post hoc test. Significant differences ( q ≤ 0.05): * vs. normoxic-counterpart, # vs. vehicle-counterpart.
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Melatonin alters oxidative balance during both normoxic and hypoxic gestations. Representative photographs ( A ) and protein levels of catalase (CAT; ( B )), glutathione peroxidase 1/2 <t>(GPX1/2;</t> ( C )), superoxide dismutase-1 (SOD1; ( D )), superoxide dismutase-2 (SOD2; ( E )) and hemeoxigenase-1 (HO-1; ( F )) in left ventricle from newborn guinea pigs exposed to gestational normoxia (N; red) or hypoxia (H; blue) and treated with vehicle or melatonin during gestation. In panel ( A ), the separated band corresponds to the loading control (LC), which was used to normalize all blots based on densitometry. Values are expressed as mean ± SEM. n = 5–6 independent samples (one per pup) per group. Data were analyzed using two-way ANOVA with FDR post hoc test. Significant differences ( q ≤ 0.05): * vs. normoxic-counterpart, # vs. vehicle-counterpart.
Gpx 1 2 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gpx 1 2 antibody/product/Santa Cruz Biotechnology
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Melatonin alters oxidative balance during both normoxic and hypoxic gestations. Representative photographs ( A ) and protein levels of catalase (CAT; ( B )), glutathione peroxidase 1/2 (GPX1/2; ( C )), superoxide dismutase-1 (SOD1; ( D )), superoxide dismutase-2 (SOD2; ( E )) and hemeoxigenase-1 (HO-1; ( F )) in left ventricle from newborn guinea pigs exposed to gestational normoxia (N; red) or hypoxia (H; blue) and treated with vehicle or melatonin during gestation. In panel ( A ), the separated band corresponds to the loading control (LC), which was used to normalize all blots based on densitometry. Values are expressed as mean ± SEM. n = 5–6 independent samples (one per pup) per group. Data were analyzed using two-way ANOVA with FDR post hoc test. Significant differences ( q ≤ 0.05): * vs. normoxic-counterpart, # vs. vehicle-counterpart.

Journal: Antioxidants

Article Title: Prenatal Melatonin Modulates Cardiovascular Function and Oxidative Stress in Guinea Pig Neonates Under Normoxic and Hypoxic Gestation

doi: 10.3390/antiox15020162

Figure Lengend Snippet: Melatonin alters oxidative balance during both normoxic and hypoxic gestations. Representative photographs ( A ) and protein levels of catalase (CAT; ( B )), glutathione peroxidase 1/2 (GPX1/2; ( C )), superoxide dismutase-1 (SOD1; ( D )), superoxide dismutase-2 (SOD2; ( E )) and hemeoxigenase-1 (HO-1; ( F )) in left ventricle from newborn guinea pigs exposed to gestational normoxia (N; red) or hypoxia (H; blue) and treated with vehicle or melatonin during gestation. In panel ( A ), the separated band corresponds to the loading control (LC), which was used to normalize all blots based on densitometry. Values are expressed as mean ± SEM. n = 5–6 independent samples (one per pup) per group. Data were analyzed using two-way ANOVA with FDR post hoc test. Significant differences ( q ≤ 0.05): * vs. normoxic-counterpart, # vs. vehicle-counterpart.

Article Snippet: Glutathione Peroxidase 1/2 , GPX1/2 , 1:2000 , #sc-133160, Santa Cruz Biotechnology, Dallas, USA , 100729115; 100714682.

Techniques: Control