gps m mutagenesis kit  (New England Biolabs)


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    Structured Review

    New England Biolabs gps m mutagenesis kit
    Gps M Mutagenesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gps m mutagenesis kit/product/New England Biolabs
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gps m mutagenesis kit - by Bioz Stars, 2020-01
    78/100 stars

    Related Products / Commonly Used Together

    vector pgem-t
    pcr
    in vitro transposition

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    Amplification:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    In Vitro:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    Mutagenesis:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    Polymerase Chain Reaction:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    Modification:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    Transformation Assay:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. Transposon-mutagenized plasmids were transformed into DH5α and transformants were selected on LB + Amp + Kan plates.

    Plasmid Preparation:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

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  • 78
    New England Biolabs gps m mutagenesis kit
    Gps M Mutagenesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gps m mutagenesis kit/product/New England Biolabs
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gps m mutagenesis kit - by Bioz Stars, 2020-01
    78/100 stars
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