in vitro transposition  (New England Biolabs)


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    Structured Review

    New England Biolabs in vitro transposition
    In Vitro Transposition, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro transposition/product/New England Biolabs
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    in vitro transposition - by Bioz Stars, 2020-08
    88/100 stars

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    Amplification:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    In Vitro:

    Article Title: Methylation of Inorganic and Organic Selenium by the Bacterial Thiopurine Methyltransferase
    Article Snippet: .. In vitro transposition was performed with the GPS-M mutagenesis system following the manufacturer's recommendations (New England Biolabs, Beverly, Mass.). .. Using this system, 190 mutagenized pAV257 cosmids subcloned into E. coli DH10B (Gibco BRL, Cergy Pontoise, France) were obtained and were then screened on 4-ppm potassium tellurite to identify tpm -inactivated Tels cosmid clones.

    Article Title: mRNA Differential Display in a Microbial Enrichment Culture: Simultaneous Identification of Three Cyclohexanone Monooxygenases from Three Species
    Article Snippet: .. Plasmids carrying the correct sequence were disrupted by in vitro transposition using the GPS-1 genome priming system kit (New England Biolabs, Inc.). .. Plasmids carrying randomly inserted transposons were sequenced from each end of the transposon to obtain the sequence of kilobase-long DNA fragments.

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    Article Title: Genetic Evidence that the uvsE Gene Product of Deinococcus radiodurans R1 Is a UV Damage Endonuclease
    Article Snippet: .. In vitro transposition was performed using the protocol developed by New England Biolabs for the GPS-M mutagenesis system. .. Twenty nanograms of purified, circular pGTC101 was combined with the TnsABC* transposase supplied with the system ( ) and target DNA (p dr1819 ).

    Mutagenesis:

    Article Title: Methylation of Inorganic and Organic Selenium by the Bacterial Thiopurine Methyltransferase
    Article Snippet: .. In vitro transposition was performed with the GPS-M mutagenesis system following the manufacturer's recommendations (New England Biolabs, Beverly, Mass.). .. Using this system, 190 mutagenized pAV257 cosmids subcloned into E. coli DH10B (Gibco BRL, Cergy Pontoise, France) were obtained and were then screened on 4-ppm potassium tellurite to identify tpm -inactivated Tels cosmid clones.

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    Article Title: Genetic Evidence that the uvsE Gene Product of Deinococcus radiodurans R1 Is a UV Damage Endonuclease
    Article Snippet: .. In vitro transposition was performed using the protocol developed by New England Biolabs for the GPS-M mutagenesis system. .. Twenty nanograms of purified, circular pGTC101 was combined with the TnsABC* transposase supplied with the system ( ) and target DNA (p dr1819 ).

    Sequencing:

    Article Title: mRNA Differential Display in a Microbial Enrichment Culture: Simultaneous Identification of Three Cyclohexanone Monooxygenases from Three Species
    Article Snippet: .. Plasmids carrying the correct sequence were disrupted by in vitro transposition using the GPS-1 genome priming system kit (New England Biolabs, Inc.). .. Plasmids carrying randomly inserted transposons were sequenced from each end of the transposon to obtain the sequence of kilobase-long DNA fragments.

    Polymerase Chain Reaction:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

    Plasmid Preparation:

    Article Title: Functional analysis of Candida albicans GPI-anchored proteins: Roles in cell wall integrity and caspofungin sensitivity
    Article Snippet: .. The open reading frame (ORF) of all genes to be disrupted was amplified by PCR and ligated into the vector pGEM-T (Promega, Madison, WI, USA) to generate the target plasmid for subsequent in vitro transposition using the GPS-M mutagenesis kit (New England Biolabs, Beverly, MA, USA, see ). .. The donor plasmid, pAED98 contains Tn7 -UAU1 , a modified Tn7 transposon carrying the UAU1 cassette ( ).

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  • 85
    New England Biolabs gps m mutagenesis kit
    Gps M Mutagenesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gps m mutagenesis kit/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gps m mutagenesis kit - by Bioz Stars, 2020-08
    85/100 stars
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