gotaq long range pcr master mix  (Promega)

 
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    Name:
    GoTaq Long PCR Master Mix
    Description:
    Hot start master mix for efficient amplification of long DNA amplicons
    Catalog Number:
    m4021
    Price:
    None
    Category:
    Nucleic Acid Extraction Analysis PCR Endpoint PCR
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    Structured Review

    Promega gotaq long range pcr master mix
    Hot start master mix for efficient amplification of long DNA amplicons
    https://www.bioz.com/result/gotaq long range pcr master mix/product/Promega
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gotaq long range pcr master mix - by Bioz Stars, 2021-03
    97/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptomics Analysis of Circular RNAs Differentially Expressed in Apoptotic HeLa Cells
    Article Snippet: The purified fragments were cloned into the pCR® II TA vector using the TA cloning kit (Thermo Fisher Scientific, United States) and sequenced at the BIOMER center (IZTECH, Turkey) to validate the backsplice junction of target circRNAs. .. qPCR Analyses cDNAs prepared from RNAse R+ and RNAseR- RNA samples were used as templates for qPCR with GoTaq q-PCR Master Mix (Promega, United States). .. Two step PCR amplification conditions were as follows: initial denaturation at 95 °C for 2 min, (40 cycles) denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min.

    Article Title: Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia
    Article Snippet: RNA Extraction, qRT-PCR and RNA-seq RNA was extracted from tissue and cells using the Maxwell 16 LEV Simply RNA purification kit (Promega) according to the instructions of the manufacturer. .. RNA quality was measured using the 2100 BioAnalyzer (Agilent). cDNA synthesis was carried out using GoScript (Promega) and qRT-PCR was performed using the GoTaq qRT-PCR master mix (Promega) with the ViiA7 Real Time PCR system (Applied Biosystem). ..

    Article Title: Differential isoform expression and alternative splicing in sex determination in mice
    Article Snippet: .. All qPCR reactions were carried out in duplicate in the Rotorgene 6000 Real Time Cycler™ (Corbett Research, Sydney, Australia) using a PCR mix (GoTaq® qPCR Master Mix, Promega Corporation, MA, USA) containing the specific primers selected for the genes (see Additional file ). ..

    Article Title: MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis
    Article Snippet: .. One microliter of cDNA was used for real-time PCR with GoTaq qRT-PCR Master Mix (Promega, USA). .. For each sample, the relative mRNA level was normalized to β-actin expression. lists the primers for qRT-PCR.

    Quantitative RT-PCR:

    Article Title: CGRP Signaling via CALCRL Increases Chemotherapy Resistance and Stem Cell Properties in Acute Myeloid Leukemia
    Article Snippet: Concerning human samples, qRT-PCR was performed using TaqMan Gene Expression Master Mix and TaqMan probes (CALCRL : Hs00907738_m1, RAMP1 : Hs00195288_m1, RAMP2 : Hs01594524_m1, RAMP3 : Hs00389131_m1, and hβ -2-microglobulin: Hs99999907_m1, Thermo Fisher). .. Regarding mouse samples, GoTaq qRT-PCR master mix (Promega, Fitchburg, WI, USA) was used together with primers Calcrl -fwd, 5′-GCAGCAGCTACCTAGCTTGAA-3′, Calcrl -rev, 5′-TTCACGCCTTCTTCCGACTC-3′, Ramp1 -fwd, 5′-GGGAAGACGCTATGGTGTGA-3′, Ramp1 -rev, 5′-TTCCGGATTGGGCCAGAAAC-3′, mβ -2-microglobulin-fwd, 5′-CCTTCAGCAAGGACTGGTCT-3′, and mβ -2-microglobulin-rev, 5′-TGTCTCGATCCCAGTAGACG-3′. .. All assays were performed in triplicate on a Step One Plus Real Time PCR system (Life Technologies), and expression of the genes of interest was normalized to the expression of β -2-microglobulin and to a calibrator sample using the ΔΔCT method [ ].

    Article Title: Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia
    Article Snippet: RNA Extraction, qRT-PCR and RNA-seq RNA was extracted from tissue and cells using the Maxwell 16 LEV Simply RNA purification kit (Promega) according to the instructions of the manufacturer. .. RNA quality was measured using the 2100 BioAnalyzer (Agilent). cDNA synthesis was carried out using GoScript (Promega) and qRT-PCR was performed using the GoTaq qRT-PCR master mix (Promega) with the ViiA7 Real Time PCR system (Applied Biosystem). ..

    Article Title: The genome-wide identification and transcriptional levels of DNA methyltransferases and demethylases in globe artichoke
    Article Snippet: .. Each 20 μL reaction was based on GoTaq® qRT-PCR Master Mix (Promega, Madison USA). .. The amplification protocol comprised an initial denaturation of 95°C/5 min, followed by 40 cycles of 95°C/5s and 60°C/45s.

    Article Title: MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis
    Article Snippet: .. One microliter of cDNA was used for real-time PCR with GoTaq qRT-PCR Master Mix (Promega, USA). .. For each sample, the relative mRNA level was normalized to β-actin expression. lists the primers for qRT-PCR.

    Article Title: Ovine Hair Follicle Stem Cells Derived from Single Vibrissae Reconstitute Haired Skin
    Article Snippet: RNA Purification, Reverse Transcription and qRT-PCR P3 and P10 ovine bulge-derived keratinocytes, as well as ovine ear-rim dermal fibroblasts, which were used as a negative control, were harvested, and total RNAs were extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. After the removal of genomic DNA with RQ1 RNase-Free DNase (Promega, Madison, WI, USA), the reverse transcription was performed with M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA, USA). qRT-PCR reactions were performed in 20-μL volumes using GoTaq qRT-PCR master mix (Promega, Madison, WI, USA) on a Roche LightCycler480 system (Roche Applied Science, Indianapolis, IN, USA). .. The qRT-PCR procedure was as follows: 94 °C for 2 min, 40 cycles of 95 °C for 15 s, 55 °C for 15 s and 68 °C for 25 s. The primers used are listed in .

    Polymerase Chain Reaction:

    Article Title: O6-Methylguanine induces altered proteins at the level of transcription in human cells
    Article Snippet: Following incubation with DpnI, linear DNA was digested using exonuclease III as described ( ). .. An aliquot of the digested DNA containing 0.05 ng (after DpnI digestion) or 0.008 ng (after DpnI and exonuclease III digestion) was used in a PCR reaction (GoTaq green master mix, Promega) containing primers (RdF: 5′-CAGCGGCCCTTCTCTCTTA-3′; RR: 5′-CGCTACAGGAACAGGTGGTG-3′) whose product spans 7 DpnI sites. .. Fluorescence microscopy For fluorescence microscopy, 2 × 105 cells were plated on collagen type I coated four-well culture slides (BD BioCoat, BD Biosciences) and transfected 24 h later with 1 µg plasmid as described above.

    Article Title: Differential isoform expression and alternative splicing in sex determination in mice
    Article Snippet: .. All qPCR reactions were carried out in duplicate in the Rotorgene 6000 Real Time Cycler™ (Corbett Research, Sydney, Australia) using a PCR mix (GoTaq® qPCR Master Mix, Promega Corporation, MA, USA) containing the specific primers selected for the genes (see Additional file ). ..

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  • 97
    Promega gotaq long pcr master mix
    Gotaq Long Pcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gotaq long pcr master mix/product/Promega
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gotaq long pcr master mix - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

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