gotaq long range pcr master mix (Promega)
97
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Name:
GoTaq Long PCR Master Mix
Description:
Hot start master mix for efficient amplification of long DNA amplicons
Catalog Number:
m4021
Price:
None
Category:
Nucleic Acid Extraction Analysis PCR Endpoint PCR
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Structured Review
Promega
gotaq long range pcr master mix
Hot start master mix for efficient amplification of long DNA amplicons
https://www.bioz.com/result/gotaq long range pcr master mix/product/Promega
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Hot start master mix for efficient amplification of long DNA amplicons
https://www.bioz.com/result/gotaq long range pcr master mix/product/Promega
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gotaq long range pcr master mix - by Bioz Stars,
2021-03
97/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Transcriptomics Analysis of Circular RNAs Differentially Expressed in Apoptotic HeLa Cells Article Snippet: The purified fragments were cloned into the pCR® II TA vector using the TA cloning kit (Thermo Fisher Scientific, United States) and sequenced at the BIOMER center (IZTECH, Turkey) to validate the backsplice junction of target circRNAs. .. qPCR Analyses cDNAs prepared from RNAse R+ and RNAseR- RNA samples were used as templates for qPCR with Article Title: Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia Article Snippet: RNA Extraction, qRT-PCR and RNA-seq RNA was extracted from tissue and cells using the Maxwell 16 LEV Simply RNA purification kit (Promega) according to the instructions of the manufacturer. .. RNA quality was measured using the 2100 BioAnalyzer (Agilent). cDNA synthesis was carried out using GoScript (Promega) and qRT-PCR was performed using the Article Title: Differential isoform expression and alternative splicing in sex determination in mice Article Snippet: .. All qPCR reactions were carried out in duplicate in the Rotorgene 6000 Real Time Cycler™ (Corbett Research, Sydney, Australia) using a Article Title: MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis Article Snippet: .. One microliter of cDNA was used for real-time PCR with Quantitative RT-PCR:Article Title: CGRP Signaling via CALCRL Increases Chemotherapy Resistance and Stem Cell Properties in Acute Myeloid Leukemia Article Snippet: Concerning human samples, qRT-PCR was performed using TaqMan Gene Expression Master Mix and TaqMan probes (CALCRL : Hs00907738_m1, RAMP1 : Hs00195288_m1, RAMP2 : Hs01594524_m1, RAMP3 : Hs00389131_m1, and hβ -2-microglobulin: Hs99999907_m1, Thermo Fisher). .. Regarding mouse samples, Article Title: Cooperative Enhancer Activation by TLX1 and STAT5 Drives Development of NUP214-ABL1/TLX1-Positive T Cell Acute Lymphoblastic Leukemia Article Snippet: RNA Extraction, qRT-PCR and RNA-seq RNA was extracted from tissue and cells using the Maxwell 16 LEV Simply RNA purification kit (Promega) according to the instructions of the manufacturer. .. RNA quality was measured using the 2100 BioAnalyzer (Agilent). cDNA synthesis was carried out using GoScript (Promega) and qRT-PCR was performed using the Article Title: The genome-wide identification and transcriptional levels of DNA methyltransferases and demethylases in globe artichoke Article Snippet: .. Each 20 μL reaction was based on Article Title: MicroRNA-27a Inhibits Cell Migration and Invasion of Fibroblast-Like Synoviocytes by Targeting Follistatin-Like Protein 1 in Rheumatoid Arthritis Article Snippet: .. One microliter of cDNA was used for real-time PCR with Article Title: Ovine Hair Follicle Stem Cells Derived from Single Vibrissae Reconstitute Haired Skin Article Snippet: RNA Purification, Reverse Transcription and qRT-PCR P3 and P10 ovine bulge-derived keratinocytes, as well as ovine ear-rim dermal fibroblasts, which were used as a negative control, were harvested, and total RNAs were extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. .. After the removal of genomic DNA with RQ1 RNase-Free DNase (Promega, Madison, WI, USA), the reverse transcription was performed with M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA, USA). qRT-PCR reactions were performed in 20-μL volumes using Polymerase Chain Reaction:Article Title: O6-Methylguanine induces altered proteins at the level of transcription in human cells Article Snippet: Following incubation with DpnI, linear DNA was digested using exonuclease III as described ( ). .. An aliquot of the digested DNA containing 0.05 ng (after DpnI digestion) or 0.008 ng (after DpnI and exonuclease III digestion) was used in a Article Title: Differential isoform expression and alternative splicing in sex determination in mice Article Snippet: .. All qPCR reactions were carried out in duplicate in the Rotorgene 6000 Real Time Cycler™ (Corbett Research, Sydney, Australia) using a |