golden gate assembly kit  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    New England Biolabs golden gate assembly kit
    <t>Golden</t> <t>Gate</t> <t>Assembly</t> of genome-editing plasmids and software to design ODN sequences. (A) Process to construct a genome-editing plasmid by Golden Gate Assembly. This panel shows an example for SpCas9 + pGAL1-sgRNA system. HH, hammerhead ribozyme-encoding sequence. (B) Screenshot of the software to automatically design the ODN sequences for Golden Gate Assembly. Upon inputting target sequences with their names to the box at the top followed by the selection of a backbone vector at the middle part, ODN sequences are displayed in the box at the bottom.
    Golden Gate Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/golden gate assembly kit/product/New England Biolabs
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    golden gate assembly kit - by Bioz Stars, 2022-11
    95/100 stars

    Images

    1) Product Images from "Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae"

    Article Title: Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae

    Journal: bioRxiv

    doi: 10.1101/2021.05.07.443192

    Golden Gate Assembly of genome-editing plasmids and software to design ODN sequences. (A) Process to construct a genome-editing plasmid by Golden Gate Assembly. This panel shows an example for SpCas9 + pGAL1-sgRNA system. HH, hammerhead ribozyme-encoding sequence. (B) Screenshot of the software to automatically design the ODN sequences for Golden Gate Assembly. Upon inputting target sequences with their names to the box at the top followed by the selection of a backbone vector at the middle part, ODN sequences are displayed in the box at the bottom.
    Figure Legend Snippet: Golden Gate Assembly of genome-editing plasmids and software to design ODN sequences. (A) Process to construct a genome-editing plasmid by Golden Gate Assembly. This panel shows an example for SpCas9 + pGAL1-sgRNA system. HH, hammerhead ribozyme-encoding sequence. (B) Screenshot of the software to automatically design the ODN sequences for Golden Gate Assembly. Upon inputting target sequences with their names to the box at the top followed by the selection of a backbone vector at the middle part, ODN sequences are displayed in the box at the bottom.

    Techniques Used: Software, Construct, Plasmid Preparation, Sequencing, Selection

    2) Product Images from "Rapid and robust assembly and decoding of molecular tags with DNA-based nanopore signatures"

    Article Title: Rapid and robust assembly and decoding of molecular tags with DNA-based nanopore signatures

    Journal: Nature Communications

    doi: 10.1038/s41467-020-19151-8

    Molbit design scheme. a Molecular bit (molbit) structure. The molbit sequence is attached to a spacer sequence via Golden Gate assembly to achieve a minimum length for sequencing and provide an additional encoding channel. Since the sequencing adapter is attached to both ends, the strand can be sensed from either direction. b The letters “UW” depicted visually in nanopore raw data (as opposed to encoded in the sequence contents). From top to bottom, the shown sequence was simulated using Scrappie and sequenced on the ONT MinION, demonstrating the viability of using simulations for designing intentional, arbitrary raw signal shapes. c Evolutionary model workflow. Each round of evolution begins with a set of sequences, their simulated squiggles, and pairwise Dynamic Time Warping (DTW) distances. The sequence order is randomized, and sequences are mutated one at a time, verifying DTW improvement (minimum and mean) after each attempt. d Dynamic time warping (DTW) scores before (left) and after (right) 31 iterations of the evolutionary model. After initialization, the minimum DTW similarity was 2.9 (mean 4.2 ± 0.4), and after evolution the minimum was 4.2 (mean 5.8 ± 0.8). Source data are provided as a Source Data file.
    Figure Legend Snippet: Molbit design scheme. a Molecular bit (molbit) structure. The molbit sequence is attached to a spacer sequence via Golden Gate assembly to achieve a minimum length for sequencing and provide an additional encoding channel. Since the sequencing adapter is attached to both ends, the strand can be sensed from either direction. b The letters “UW” depicted visually in nanopore raw data (as opposed to encoded in the sequence contents). From top to bottom, the shown sequence was simulated using Scrappie and sequenced on the ONT MinION, demonstrating the viability of using simulations for designing intentional, arbitrary raw signal shapes. c Evolutionary model workflow. Each round of evolution begins with a set of sequences, their simulated squiggles, and pairwise Dynamic Time Warping (DTW) distances. The sequence order is randomized, and sequences are mutated one at a time, verifying DTW improvement (minimum and mean) after each attempt. d Dynamic time warping (DTW) scores before (left) and after (right) 31 iterations of the evolutionary model. After initialization, the minimum DTW similarity was 2.9 (mean 4.2 ± 0.4), and after evolution the minimum was 4.2 (mean 5.8 ± 0.8). Source data are provided as a Source Data file.

    Techniques Used: Sequencing

    3) Product Images from "Porcupine: Rapid and robust tagging of physical objects using nanopore-orthogonal DNA strands"

    Article Title: Porcupine: Rapid and robust tagging of physical objects using nanopore-orthogonal DNA strands

    Journal: bioRxiv

    doi: 10.1101/2020.03.06.981514

    Molbit design scheme. (a) Molecular bit (molbit) structure. The molbit sequence is attached to a spacer sequence via Golden Gate assembly to achieve a minimum length for sequencing and provide an additional encoding channel. Since the sequencing adapter is attached to both ends, the strand can be sensed from either direction. (b) The letters “UW” depicted visually in nanopore raw data (as opposed to encoded in the sequence contents). From top to bottom, the shown sequence was simulated using Scrappie and sequenced on the ONT Min-ION, demonstrating the viability of using simulations for designing intentional, arbitrary raw signal shapes. (c) Evolutionary model workflow. Each round of evolution begins with a set of sequences, their simulated squiggles, and pairwise Dynamic Time Warping (DTW) distances. The sequence order is randomized, and sequences are mutated one at a time, verifying DTW improvement (minimum and mean) after each attempt. (d) Dynamic time warping (DTW) scores before (left) and after (right) 31 iterations of the evolutionary model. After initialization, the minimum DTW similarity was 2.9 (mean 4.2 +/- 0.4), and after evolution the minimum was 4.2 (mean 5.8 +/- 0.8).
    Figure Legend Snippet: Molbit design scheme. (a) Molecular bit (molbit) structure. The molbit sequence is attached to a spacer sequence via Golden Gate assembly to achieve a minimum length for sequencing and provide an additional encoding channel. Since the sequencing adapter is attached to both ends, the strand can be sensed from either direction. (b) The letters “UW” depicted visually in nanopore raw data (as opposed to encoded in the sequence contents). From top to bottom, the shown sequence was simulated using Scrappie and sequenced on the ONT Min-ION, demonstrating the viability of using simulations for designing intentional, arbitrary raw signal shapes. (c) Evolutionary model workflow. Each round of evolution begins with a set of sequences, their simulated squiggles, and pairwise Dynamic Time Warping (DTW) distances. The sequence order is randomized, and sequences are mutated one at a time, verifying DTW improvement (minimum and mean) after each attempt. (d) Dynamic time warping (DTW) scores before (left) and after (right) 31 iterations of the evolutionary model. After initialization, the minimum DTW similarity was 2.9 (mean 4.2 +/- 0.4), and after evolution the minimum was 4.2 (mean 5.8 +/- 0.8).

    Techniques Used: Sequencing

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    New England Biolabs monarch pcr and dna cleanup kit
    Monarch Pcr And Dna Cleanup Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monarch pcr and dna cleanup kit/product/New England Biolabs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monarch pcr and dna cleanup kit - by Bioz Stars, 2022-11
    88/100 stars
      Buy from Supplier

    95
    New England Biolabs neb golden gate assembly mix
    Neb Golden Gate Assembly Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb golden gate assembly mix/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neb golden gate assembly mix - by Bioz Stars, 2022-11
    95/100 stars
      Buy from Supplier

    94
    New England Biolabs neb golden gate assembly kit
    Neb Golden Gate Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb golden gate assembly kit/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    neb golden gate assembly kit - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

    Image Search Results